2JIX

Key:

Heavy Chains
Light Chains
Bound Antigen Chains
Chothia CDRs
Other

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Structure information

Crystal Structure Of Abt-007 Fab Fragment With The Soluble Domain Of Epo Receptor

Item	Info
PDB code:	2jix
Organism:	SYNTHETIC CONSTRUCT
Experimental method:	X-RAY DIFFRACTION
In complex:	True
Fvs present:	3
Binding affinity (KD):	3e-08 M
ΔG:	-10.26 kcal/mol
Affinity method:	SPR
Source:	Paper Link
Light chain type:	Kappa
PDB has constant region:	True

Paired chains information

This PDB has 3 antibody variable fragment(s)

D / G

Fab Details:

Item	Info
Heavy Chain	D
Light Chain	G
Heavy V / J / C subgroups	IGHV4 / IGHJ4 / IGHG1
Light V / J / C subgroups	IGKV1 / IGKJ1 / IGKC
Bound-state	Bound
Species	SYNTHETIC CONSTRUCT
ScFv?	False
Constant domains present?	True

Antigen Details:

Item	Info
Chain	E
Type	protein
Name	erythropoietin receptor
Species	HOMO SAPIENS

Sequence:

Chothia-numbered antibody sequences

Chain type

Chain id

Fasta sequence

Chothia annotated sequence ( framework / CDRs )

VH

D

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H1	H2	H3	H4	H5	H6	H7	H8	H9	H10
Q	V	Q	L	Q	E	S	G	P	G

H11	H12	H13	H14	H15	H16	H17	H18	H19	H20
L	V	K	P	S	E	T	L	S	L

H21	H22	H23	H24	H25	H26	H27	H28	H29	H30
T	C	T	V	S	G	A	S	I	S

H31	H32	H33	H34	H35	H36	H37	H38	H39	H40
S	Y	Y	W	S	W	I	R	Q	P

H41	H42	H43	H44	H45	H46	H47	H48	H49	H50
P	G	K	G	L	E	W	I	G	Y

H51	H52	H53	H54	H55	H56	H57	H58	H59	H60
I	G	G	E	G	S	T	N	Y	N

H61	H62	H63	H64	H65	H66	H67	H68	H69	H70
P	S	L	K	S	R	V	T	I	S

H71	H72	H73	H74	H75	H76	H77	H78	H79	H80
V	D	T	S	K	N	Q	F	S	L

H81	H82	H82A	H82B	H82C	H83	H84	H85	H86	H87
K	L	R	S	V	T	A	A	D	T

H88	H89	H90	H91	H92	H93	H94	H95	H96	H97
A	V	Y	Y	C	A	R	E	R	L

H98	H99	H100	H101	H102	H103	H104	H105	H106	H107
G	I	G	D	Y	W	G	Q	G	T

H108	H109	H110	H111	H112	H113
L	V	T	V	S	S

VL

G

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L1	L2	L3	L4	L5	L6	L7	L8	L9	L10
D	I	Q	L	T	Q	S	P	S	S

L11	L12	L13	L14	L15	L16	L17	L18	L19	L20
L	S	A	S	V	G	D	R	V	T

L21	L22	L23	L24	L25	L26	L27	L28	L29	L30
I	T	C	R	A	S	Q	G	I	R

L31	L32	L33	L34	L35	L36	L37	L38	L39	L40
N	D	L	G	W	Y	Q	Q	K	P

L41	L42	L43	L44	L45	L46	L47	L48	L49	L50
G	K	A	P	K	R	L	I	Y	A

L51	L52	L53	L54	L55	L56	L57	L58	L59	L60
A	S	S	L	Q	S	G	V	P	S

L61	L62	L63	L64	L65	L66	L67	L68	L69	L70
R	F	S	G	S	G	S	G	T	E

L71	L72	L73	L74	L75	L76	L77	L78	L79	L80
F	T	L	T	I	S	S	L	Q	P

L81	L82	L83	L84	L85	L86	L87	L88	L89	L90
E	D	F	A	T	Y	Y	C	L	Q

L91	L92	L93	L94	L95	L96	L97	L98	L99	L100
H	N	T	Y	P	P	T	F	G	Q

L101	L102	L103	L104	L105	L106	L107	L108	L109	L110
G	T	K	V	E	I	K	R	T	V

Antigen sequence

Antigen type	Chain id	Fasta sequence	Sequence (Clustal colouring)
protein	E	Download	A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D

Antibody i-Patch annotation:

Fab heavy/lightchain	Link to the Antibody i-Patch annotation (What is this?)
D/G	Get AB i-Patch annotation

Chothia CDR information:

CDR	Sequence
CDRL1	RASQGIRNDLG
CDRL2	AASSLQS
CDRL3	LQHNTYPPT
CDRH1	GASISSY
CDRH2	GGEGS
CDRH3	ERLGIGDY

VH-VL orientation:
(see plot)

Angle	Value
HL	-63.7°
HC1	70.5°
HC2	116.7°
LC1	119.6°
LC2	84.2°
dc	16.1 Å

F / A

Fab Details:

Item	Info
Heavy Chain	F
Light Chain	A
Heavy V / J / C subgroups	IGHV4 / IGHJ4 / IGHG1
Light V / J / C subgroups	IGKV1 / IGKJ1 / IGKC
Bound-state	Bound
Species	SYNTHETIC CONSTRUCT
ScFv?	False
Constant domains present?	True

Antigen Details:

Item	Info
Chain	C; B
Type	protein
Name	erythropoietin receptor
Species	HOMO SAPIENS

Sequence:

Chothia-numbered antibody sequences

Chain type

Chain id

Fasta sequence

Chothia annotated sequence ( framework / CDRs )

VH

F

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H1	H2	H3	H4	H5	H6	H7	H8	H9	H10
Q	V	Q	L	Q	E	S	G	P	G

H11	H12	H13	H14	H15	H16	H17	H18	H19	H20
L	V	K	P	S	E	T	L	S	L

H21	H22	H23	H24	H25	H26	H27	H28	H29	H30
T	C	T	V	S	G	A	S	I	S

H31	H32	H33	H34	H35	H36	H37	H38	H39	H40
S	Y	Y	W	S	W	I	R	Q	P

H41	H42	H43	H44	H45	H46	H47	H48	H49	H50
P	G	K	G	L	E	W	I	G	Y

H51	H52	H53	H54	H55	H56	H57	H58	H59	H60
I	G	G	E	G	S	T	N	Y	N

H61	H62	H63	H64	H65	H66	H67	H68	H69	H70
P	S	L	K	S	R	V	T	I	S

H71	H72	H73	H74	H75	H76	H77	H78	H79	H80
V	D	T	S	K	N	Q	F	S	L

H81	H82	H82A	H82B	H82C	H83	H84	H85	H86	H87
K	L	R	S	V	T	A	A	D	T

H88	H89	H90	H91	H92	H93	H94	H95	H96	H97
A	V	Y	Y	C	A	R	E	R	L

H98	H99	H100	H101	H102	H103	H104	H105	H106	H107
G	I	G	D	Y	W	G	Q	G	T

H108	H109	H110	H111	H112	H113
L	V	T	V	S	S

VL

A

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L1	L2	L3	L4	L5	L6	L7	L8	L9	L10
D	I	Q	L	T	Q	S	P	S	S

L11	L12	L13	L14	L15	L16	L17	L18	L19	L20
L	S	A	S	V	G	D	R	V	T

L21	L22	L23	L24	L25	L26	L27	L28	L29	L30
I	T	C	R	A	S	Q	G	I	R

L31	L32	L33	L34	L35	L36	L37	L38	L39	L40
N	D	L	G	W	Y	Q	Q	K	P

L41	L42	L43	L44	L45	L46	L47	L48	L49	L50
G	K	A	P	K	R	L	I	Y	A

L51	L52	L53	L54	L55	L56	L57	L58	L59	L60
A	S	S	L	Q	S	G	V	P	S

L61	L62	L63	L64	L65	L66	L67	L68	L69	L70
R	F	S	G	S	G	S	G	T	E

L71	L72	L73	L74	L75	L76	L77	L78	L79	L80
F	T	L	T	I	S	S	L	Q	P

L81	L82	L83	L84	L85	L86	L87	L88	L89	L90
E	D	F	A	T	Y	Y	C	L	Q

L91	L92	L93	L94	L95	L96	L97	L98	L99	L100
H	N	T	Y	P	P	T	F	G	Q

L101	L102	L103	L104	L105	L106	L107	L108	L109	L110
G	T	K	V	E	I	K	R	T	V

Antigen sequence

Antigen type	Chain id	Fasta sequence	Sequence (Clustal colouring)
protein	C	Download	A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D
protein	B	Download	A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D

Antibody i-Patch annotation:

Fab heavy/lightchain	Link to the Antibody i-Patch annotation (What is this?)
F/A	Get AB i-Patch annotation

Chothia CDR information:

CDR	Sequence
CDRL1	RASQGIRNDLG
CDRL2	AASSLQS
CDRL3	LQHNTYPPT
CDRH1	GASISSY
CDRH2	GGEGS
CDRH3	ERLGIGDY

VH-VL orientation:
(see plot)

Angle	Value
HL	-62.2°
HC1	72.2°
HC2	117.1°
LC1	119.9°
LC2	83.8°
dc	16.0 Å

H / L

Fab Details:

Item	Info
Heavy Chain	H
Light Chain	L
Heavy V / J / C subgroups	IGHV4 / IGHJ4 / IGHG1
Light V / J / C subgroups	IGKV1 / IGKJ1 / IGKC
Bound-state	Bound
Species	SYNTHETIC CONSTRUCT
ScFv?	False
Constant domains present?	True

Antigen Details:

Item	Info
Chain	E
Type	protein
Name	erythropoietin receptor
Species	HOMO SAPIENS

Sequence:

Chothia-numbered antibody sequences

Chain type

Chain id

Fasta sequence

Chothia annotated sequence ( framework / CDRs )

VH

H

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H1	H2	H3	H4	H5	H6	H7	H8	H9	H10
Q	V	Q	L	Q	E	S	G	P	G

H11	H12	H13	H14	H15	H16	H17	H18	H19	H20
L	V	K	P	S	E	T	L	S	L

H21	H22	H23	H24	H25	H26	H27	H28	H29	H30
T	C	T	V	S	G	A	S	I	S

H31	H32	H33	H34	H35	H36	H37	H38	H39	H40
S	Y	Y	W	S	W	I	R	Q	P

H41	H42	H43	H44	H45	H46	H47	H48	H49	H50
P	G	K	G	L	E	W	I	G	Y

H51	H52	H53	H54	H55	H56	H57	H58	H59	H60
I	G	G	E	G	S	T	N	Y	N

H61	H62	H63	H64	H65	H66	H67	H68	H69	H70
P	S	L	K	S	R	V	T	I	S

H71	H72	H73	H74	H75	H76	H77	H78	H79	H80
V	D	T	S	K	N	Q	F	S	L

H81	H82	H82A	H82B	H82C	H83	H84	H85	H86	H87
K	L	R	S	V	T	A	A	D	T

H88	H89	H90	H91	H92	H93	H94	H95	H96	H97
A	V	Y	Y	C	A	R	E	R	L

H98	H99	H100	H101	H102	H103	H104	H105	H106	H107
G	I	G	D	Y	W	G	Q	G	T

H108	H109	H110	H111	H112	H113
L	V	T	V	S	S

VL

L

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L1	L2	L3	L4	L5	L6	L7	L8	L9	L10
D	I	Q	L	T	Q	S	P	S	S

L11	L12	L13	L14	L15	L16	L17	L18	L19	L20
L	S	A	S	V	G	D	R	V	T

L21	L22	L23	L24	L25	L26	L27	L28	L29	L30
I	T	C	R	A	S	Q	G	I	R

L31	L32	L33	L34	L35	L36	L37	L38	L39	L40
N	D	L	G	W	Y	Q	Q	K	P

L41	L42	L43	L44	L45	L46	L47	L48	L49	L50
G	K	A	P	K	R	L	I	Y	A

L51	L52	L53	L54	L55	L56	L57	L58	L59	L60
A	S	S	L	Q	S	G	V	P	S

L61	L62	L63	L64	L65	L66	L67	L68	L69	L70
R	F	S	G	S	G	S	G	T	E

L71	L72	L73	L74	L75	L76	L77	L78	L79	L80
F	T	L	T	I	S	S	L	Q	P

L81	L82	L83	L84	L85	L86	L87	L88	L89	L90
E	D	F	A	T	Y	Y	C	L	Q

L91	L92	L93	L94	L95	L96	L97	L98	L99	L100
H	N	T	Y	P	P	T	F	G	Q

L101	L102	L103	L104	L105	L106	L107	L108	L109	L110
G	T	K	V	E	I	K	R	T	V

Antigen sequence

Antigen type	Chain id	Fasta sequence	Sequence (Clustal colouring)
protein	E	Download	A P P P N L P D P K F E S K A A L L A A R G P E E L L C F T E R L E D L V C F W E E A A S A G V G P G N Y S F S Y Q L E D E P W K L C R L H Q A P T A R G A V R F W C S L P T A D T S S F V P L E L R V T A A S G A P R Y H R V I H I N E V V L L D A P V G L V A R L A D E S G H V V L R W L P P P E T P M T S H I R Y E V D V S A G N G A G S V Q R V E I L E G R T E C V L S N L R G R T R Y T F A V R A R M A E P S F G G F W S A W S E P V S L L T P S D L D

Antibody i-Patch annotation:

Fab heavy/lightchain	Link to the Antibody i-Patch annotation (What is this?)
H/L	Get AB i-Patch annotation

Chothia CDR information:

CDR	Sequence
CDRL1	RASQGIRNDLG
CDRL2	AASSLQS
CDRL3	LQHNTYPPT
CDRH1	GASISSY
CDRH2	GGEGS
CDRH3	ERLGIGDY

VH-VL orientation:
(see plot)

Angle	Value
HL	-60.0°
HC1	72.0°
HC2	117.5°
LC1	120.9°
LC2	85.1°
dc	16.0 Å

Downloads

Downloads and links.

Item	Download link
Full structure	Download
Chothia structure	Download
Summary file	Download
Entry in the PDB	See the file on the PDB
Entry in IMGT	See the IMGT entry

Structure summary for 2JIX

Details of structure 2JIX

Structure visualisation for 2JIX

Structure information

Paired chains information

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