Structure summary for 3IU3

Detailed entry information.

Key:

Heavy Chains
Light Chains
Bound Antigen Chains
Chothia CDRs
Other

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Crystal Structure Of The Fab Fragment Of Therapeutic Antibody Basiliximab In Complex With Il-2Ra (Cd25) Ectodomain

ItemInfo
PDB code: 3iu3
Organism: SYNTHETIC CONSTRUCT
Experimental method: X-RAY DIFFRACTION
In complex: True
Fvs present: 3
Binding affinity (KD): 1.4e-10 M
ΔG: -13.44 kcal/mol
Affinity method: SPR
Temperature: 25 °C
Source: Paper Link
Light chain type: Kappa
PDB has constant region: True

This PDB has 3 antibody variable fragment(s)

Fab Details:
ItemInfo
Heavy Chain A
Light Chain B
Heavy V / J / C subgroups IGHV1 / IGHJ2 / IGHG1
Light V / J / C subgroups IGKV4 / IGKJ1 / IGKC
Bound-state Bound
Species SYNTHETIC CONSTRUCT
ScFv? False
Constant domains present? True
Antigen Details:
ItemInfo
Chain K
Type protein
Name interleukin-2 receptor alpha chain
Species HOMO SAPIENS

Sequence:
Chain typeChain idFasta sequenceChothia annotated sequence ( framework / CDRs )
VH A Download
H3 H4 H5 H6 H7 H8 H9 H10 H11 H12
QLQQSGTVLA
H13 H14 H15 H16 H17 H18 H19 H20 H21 H22
RPGASVKMSC
H23 H24 H25 H26 H27 H28 H29 H30 H31 H32
KASGYSFTRY
H33 H34 H35 H36 H37 H38 H39 H40 H41 H42
WMHWIKQRPG
H43 H44 H45 H46 H47 H48 H49 H50 H51 H52
QGLEWIGAIY
H52AH53 H54 H55 H56 H57 H58 H59 H60 H61
PGNSDTSYNQ
H62 H63 H64 H65 H66 H67 H68 H69 H70 H71
KFEGKAKLTA
H72 H73 H74 H75 H76 H77 H78 H79 H80 H81
VTSASTAYME
H82 H82AH82BH82CH83 H84 H85 H86 H87 H88
LSSLTHEDSA
H89 H90 H91 H92 H93 H94 H95 H96 H97 H98
VYYCSRDYGY
H99 H100 H101 H102 H103 H104 H105 H106 H107 H108
YFDFWGQGTT
H109 H110 H111 H112 H113
LTVSS
VL B Download
L1 L2 L3 L4 L5 L6 L7 L8 L9 L10
QIVSTQSPAI
L11 L12 L13 L14 L15 L16 L17 L18 L19 L20
MSASPGEKVT
L21 L22 L23 L24 L25 L26 L27 L28 L29 L30
MTCSASSSRS
L32 L33 L34 L35 L36 L37 L38 L39 L40 L41
YMQWYQQKPG
L42 L43 L44 L45 L46 L47 L48 L49 L50 L51
TSPKRWIYDT
L52 L53 L54 L55 L56 L57 L58 L59 L60 L61
SKLASGVPAR
L62 L63 L64 L65 L66 L67 L68 L69 L70 L71
FSGSGSGTSY
L72 L73 L74 L75 L76 L77 L78 L79 L80 L81
SLTISSMEAE
L82 L83 L84 L85 L86 L87 L88 L89 L90 L91
DAATYYCHQR
L92 L93 L96 L97 L98 L99 L100 L101 L102 L103
SSYTFGGGTK
L104 L105 L106 L107 L108 L109 L110
LEIKRTV
Antigen typeChain idFasta sequenceSequence (Clustal colouring)
protein K Download
E L C D D D P P E I P H A T F K A M A Y K E G T M L N C E C
K R G F R R I K S G S L Y M L C T G N S S H S S W D N Q C Q
C T S S A T R N T T K Q V T P Q P E E Q K E R K T T E M Q S
P M Q P V D Q A S L P G H C R E P P P W E N E A T E R I Y H
F V V G Q M V Y Y Q C V Q G Y R A L H R G P A E S V C K M T
H G K T R W T Q P Q L I C T G E M E T S Q F P G E E K P Q A
S P E G R P E S E T S C L V T T T D F Q I Q T E M A A T M E
T S I F T T E H H H H H H

Antibody i-Patch annotation:
Fab heavy/lightchainLink to the Antibody i-Patch annotation (What is this?)
A/BGet AB i-Patch annotation
Chothia CDR information:
CDRSequence
CDRL1 SASSSRSYMQ
CDRL2 DTSKLAS
CDRL3 HQRSSYT
CDRH1 GYSFTRY
CDRH2 YPGNSD
CDRH3 DYGYYFDF
VH-VL orientation:
(see plot)
AngleValue
HL -56.2°
HC1 74.3°
HC2 120.1°
LC1 120.9°
LC2 79.5°
dc 16.4 Å
Fab Details:
ItemInfo
Heavy Chain C
Light Chain D
Heavy V / J / C subgroups IGHV1 / IGHJ2 / IGHG1
Light V / J / C subgroups IGKV4 / IGKJ1 / IGKC
Bound-state Bound
Species SYNTHETIC CONSTRUCT
ScFv? False
Constant domains present? True
Antigen Details:
ItemInfo
Chain J
Type protein
Name interleukin-2 receptor alpha chain
Species HOMO SAPIENS

Sequence:
Chain typeChain idFasta sequenceChothia annotated sequence ( framework / CDRs )
VH C Download
H3 H4 H5 H6 H7 H8 H9 H10 H11 H12
QLQQSGTVLA
H13 H14 H15 H16 H17 H18 H19 H20 H21 H22
RPGASVKMSC
H23 H24 H25 H26 H27 H28 H29 H30 H31 H32
KASGYSFTRY
H33 H34 H35 H36 H37 H38 H39 H40 H41 H42
WMHWIKQRPG
H43 H44 H45 H46 H47 H48 H49 H50 H51 H52
QGLEWIGAIY
H52AH53 H54 H55 H56 H57 H58 H59 H60 H61
PGNSDTSYNQ
H62 H63 H64 H65 H66 H67 H68 H69 H70 H71
KFEGKAKLTA
H72 H73 H74 H75 H76 H77 H78 H79 H80 H81
VTSASTAYME
H82 H82AH82BH82CH83 H84 H85 H86 H87 H88
LSSLTHEDSA
H89 H90 H91 H92 H93 H94 H95 H96 H97 H98
VYYCSRDYGY
H99 H100 H101 H102 H103 H104 H105 H106 H107 H108
YFDFWGQGTT
H109 H110 H111 H112 H113
LTVSS
VL D Download
L1 L2 L3 L4 L5 L6 L7 L8 L9 L10
QIVSTQSPAI
L11 L12 L13 L14 L15 L16 L17 L18 L19 L20
MSASPGEKVT
L21 L22 L23 L24 L25 L26 L27 L28 L29 L30
MTCSASSSRS
L32 L33 L34 L35 L36 L37 L38 L39 L40 L41
YMQWYQQKPG
L42 L43 L44 L45 L46 L47 L48 L49 L50 L51
TSPKRWIYDT
L52 L53 L54 L55 L56 L57 L58 L59 L60 L61
SKLASGVPAR
L62 L63 L64 L65 L66 L67 L68 L69 L70 L71
FSGSGSGTSY
L72 L73 L74 L75 L76 L77 L78 L79 L80 L81
SLTISSMEAE
L82 L83 L84 L85 L86 L87 L88 L89 L90 L91
DAATYYCHQR
L92 L93 L96 L97 L98 L99 L100 L101 L102 L103
SSYTFGGGTK
L104 L105 L106 L107 L108 L109 L110
LEIKRTV
Antigen typeChain idFasta sequenceSequence (Clustal colouring)
protein J Download
E L C D D D P P E I P H A T F K A M A Y K E G T M L N C E C
K R G F R R I K S G S L Y M L C T G N S S H S S W D N Q C Q
C T S S A T R N T T K Q V T P Q P E E Q K E R K T T E M Q S
P M Q P V D Q A S L P G H C R E P P P W E N E A T E R I Y H
F V V G Q M V Y Y Q C V Q G Y R A L H R G P A E S V C K M T
H G K T R W T Q P Q L I C T G E M E T S Q F P G E E K P Q A
S P E G R P E S E T S C L V T T T D F Q I Q T E M A A T M E
T S I F T T E H H H H H H

Antibody i-Patch annotation:
Fab heavy/lightchainLink to the Antibody i-Patch annotation (What is this?)
C/DGet AB i-Patch annotation
Chothia CDR information:
CDRSequence
CDRL1 SASSSRSYMQ
CDRL2 DTSKLAS
CDRL3 HQRSSYT
CDRH1 GYSFTRY
CDRH2 YPGNSD
CDRH3 DYGYYFDF
VH-VL orientation:
(see plot)
AngleValue
HL -55.5°
HC1 74.2°
HC2 120.1°
LC1 121.4°
LC2 80.3°
dc 16.5 Å
Fab Details:
ItemInfo
Heavy Chain H
Light Chain L
Heavy V / J / C subgroups IGHV1 / IGHJ2 / IGHG1
Light V / J / C subgroups IGKV4 / IGKJ1 / IGKC
Bound-state Bound
Species SYNTHETIC CONSTRUCT
ScFv? False
Constant domains present? True
Antigen Details:
ItemInfo
Chain I
Type protein
Name interleukin-2 receptor alpha chain
Species HOMO SAPIENS

Sequence:
Chain typeChain idFasta sequenceChothia annotated sequence ( framework / CDRs )
VH H Download
H3 H4 H5 H6 H7 H8 H9 H10 H11 H12
QLQQSGTVLA
H13 H14 H15 H16 H17 H18 H19 H20 H21 H22
RPGASVKMSC
H23 H24 H25 H26 H27 H28 H29 H30 H31 H32
KASGYSFTRY
H33 H34 H35 H36 H37 H38 H39 H40 H41 H42
WMHWIKQRPG
H43 H44 H45 H46 H47 H48 H49 H50 H51 H52
QGLEWIGAIY
H52AH53 H54 H55 H56 H57 H58 H59 H60 H61
PGNSDTSYNQ
H62 H63 H64 H65 H66 H67 H68 H69 H70 H71
KFEGKAKLTA
H72 H73 H74 H75 H76 H77 H78 H79 H80 H81
VTSASTAYME
H82 H82AH82BH82CH83 H84 H85 H86 H87 H88
LSSLTHEDSA
H89 H90 H91 H92 H93 H94 H95 H96 H97 H98
VYYCSRDYGY
H99 H100 H101 H102 H103 H104 H105 H106 H107 H108
YFDFWGQGTT
H109 H110 H111 H112 H113
LTVSS
VL L Download
L1 L2 L3 L4 L5 L6 L7 L8 L9 L10
QIVSTQSPAI
L11 L12 L13 L14 L15 L16 L17 L18 L19 L20
MSASPGEKVT
L21 L22 L23 L24 L25 L26 L27 L28 L29 L30
MTCSASSSRS
L32 L33 L34 L35 L36 L37 L38 L39 L40 L41
YMQWYQQKPG
L42 L43 L44 L45 L46 L47 L48 L49 L50 L51
TSPKRWIYDT
L52 L53 L54 L55 L56 L57 L58 L59 L60 L61
SKLASGVPAR
L62 L63 L64 L65 L66 L67 L68 L69 L70 L71
FSGSGSGTSY
L72 L73 L74 L75 L76 L77 L78 L79 L80 L81
SLTISSMEAE
L82 L83 L84 L85 L86 L87 L88 L89 L90 L91
DAATYYCHQR
L92 L93 L96 L97 L98 L99 L100 L101 L102 L103
SSYTFGGGTK
L104 L105 L106 L107 L108 L109 L110
LEIKRTV
Antigen typeChain idFasta sequenceSequence (Clustal colouring)
protein I Download
E L C D D D P P E I P H A T F K A M A Y K E G T M L N C E C
K R G F R R I K S G S L Y M L C T G N S S H S S W D N Q C Q
C T S S A T R N T T K Q V T P Q P E E Q K E R K T T E M Q S
P M Q P V D Q A S L P G H C R E P P P W E N E A T E R I Y H
F V V G Q M V Y Y Q C V Q G Y R A L H R G P A E S V C K M T
H G K T R W T Q P Q L I C T G E M E T S Q F P G E E K P Q A
S P E G R P E S E T S C L V T T T D F Q I Q T E M A A T M E
T S I F T T E H H H H H H

Antibody i-Patch annotation:
Fab heavy/lightchainLink to the Antibody i-Patch annotation (What is this?)
H/LGet AB i-Patch annotation
Chothia CDR information:
CDRSequence
CDRL1 SASSSRSYMQ
CDRL2 DTSKLAS
CDRL3 HQRSSYT
CDRH1 GYSFTRY
CDRH2 YPGNSD
CDRH3 DYGYYFDF
VH-VL orientation:
(see plot)
AngleValue
HL -56.2°
HC1 74.2°
HC2 120.0°
LC1 121.0°
LC2 79.8°
dc 16.5 Å

Downloads and links.

ItemDownload link
Full structureDownload
Chothia structureDownload
Summary fileDownload
Entry in the PDBSee the file on the PDB
Entry in IMGTSee the IMGT entry
Flag misannotation