Structure summary for 4FAB

Detailed entry information.

Key:

Heavy Chains
Light Chains
Bound Antigen Chains
Chothia CDRs
Other

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Three-Dimensional Structure Of A Fluorescein-Fab Complex Crystallized In 2-Methyl-2,4-Pentanediol

ItemInfo
PDB code: 4fab
Organism: MUS MUSCULUS
Experimental method: X-RAY DIFFRACTION
In complex: True
Fvs present: 1
Binding affinity (KD): 8.33e-09 M
ΔG: -11.02 kcal/mol
Affinity method: Other
Source: Paper Link
Light chain type: Kappa
PDB has constant region: True

This PDB has 1 antibody variable fragment(s)

Fab Details:
ItemInfo
Heavy Chain H
Light Chain L
Heavy V / J / C subgroups IGHV6 / IGHJ4 / IGHG2A
Light V / J / C subgroups IGKV1 / IGKJ1 / IGKC
Bound-state Bound
Species MUS MUSCULUS
ScFv? False
Constant domains present? True
Antigen Details:
ItemInfo
On chain H
Type Hapten
Name FLUORESCIN

Sequence:
Chain typeChain idFasta sequenceChothia annotated sequence ( framework / CDRs )
VH H Download
H1 H2 H3 H4 H5 H6 H7 H8 H9 H10
EVKLDETGGG
H11 H12 H13 H14 H15 H16 H17 H18 H19 H20
LVQPGRPMKL
H21 H22 H23 H24 H25 H26 H27 H28 H29 H30
SCVASGFTFS
H31 H32 H33 H34 H35 H36 H37 H38 H39 H40
DYWMNWVRQS
H41 H42 H43 H44 H45 H46 H47 H48 H49 H50
PEKGLEWVAQ
H51 H52 H52AH52BH52CH53 H54 H55 H56 H57
IRNKPYNYET
H58 H59 H60 H61 H62 H63 H64 H65 H66 H67
YYSDSVKGRF
H68 H69 H70 H71 H72 H73 H74 H75 H76 H77
TISRDDSKSS
H78 H79 H80 H81 H82 H82AH82BH82CH83 H84
VYLQMNNLRV
H85 H86 H87 H88 H89 H90 H91 H92 H93 H94
EDMGIYYCTG
H95 H96 H97 H98 H99 H101 H102 H103 H104 H105
SYYGMDYWGQ
H106 H107 H108 H109 H110 H111 H112 H113
GTSVTVSS
VL L Download
L1 L2 L3 L4 L5 L6 L7 L8 L9 L10
DVVMTQTPLS
L11 L12 L13 L14 L15 L16 L17 L18 L19 L20
LPVSLGDQAS
L21 L22 L23 L24 L25 L26 L27 L28 L29 L30
ISCRSSQSLV
L30AL30BL30CL30DL30EL31 L32 L33 L34 L35
HSQGNTYLRW
L36 L37 L38 L39 L40 L41 L42 L43 L44 L45
YLQKPGQSPK
L46 L47 L48 L49 L50 L51 L52 L53 L54 L55
VLIYKVSNRF
L56 L57 L58 L59 L60 L61 L62 L63 L64 L65
SGVPDRFSGS
L66 L67 L68 L69 L70 L71 L72 L73 L74 L75
GSGTDFTLKI
L76 L77 L78 L79 L80 L81 L82 L83 L84 L85
SRVEAEDLGV
L86 L87 L88 L89 L90 L91 L92 L93 L94 L95
YFCSQSTHVP
L96 L97 L98 L99 L100 L101 L102 L103 L104 L105
WTFGGGTKLE
L106 L107 L108 L109
IKRA
Antigen typeOn chain idStructure
Hapten H

Antibody i-Patch annotation:
Fab heavy/lightchainLink to the Antibody i-Patch annotation (What is this?)
H/LGet AB i-Patch annotation
Chothia CDR information:
CDRSequence
CDRL1 RSSQSLVHSQGNTYLR
CDRL2 KVSNRFS
CDRL3 SQSTHVPWT
CDRH1 GFTFSDY
CDRH2 RNKPYNYE
CDRH3 SYYGMDY
VH-VL orientation:
(see plot)
AngleValue
HL -55.2°
HC1 70.1°
HC2 115.5°
LC1 119.9°
LC2 84.8°
dc 16.2 Å

Downloads and links.

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Full structureDownload
Chothia structureDownload
Summary fileDownload
Entry in the PDBSee the file on the PDB
Entry in IMGTSee the IMGT entry
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