Allosteric regulation is a common mechanism in the protein world but little is known about the underlying mechanisms on a residue level. This project links the analysis of allostery with the analysis of co-evolution of protein residues. Co-evolution occurs when the interaction between two (or more) residues is crucial for a protein’s stability or functionality, e.g. allosteric signal transmission. My research aims to develop a pipeline for automated allostery analysis based solely on sequence information. Currently, there are not many tools available to analyse allostery, especially no high-throughput ones, so developing and improving such basic tools could be a first step of in the process of understanding allosteric signal transmission and potentially improving combination therapies where two drugs target a protein at different sites.