T-cell receptor (TCR) cross-reactivity poses a challenge in developing safe and effective immunotherapies, as TCRs can inadvertently recognise multiple peptides presented by the Peptide-human leukocyte antigen (pHLA) proteins, leading to off-target effects. I work on studying how the structural diversity of these peptides influences TCR binding and cross-reactivity by developing and applying computational tools that identify common conformational patterns. This work aims to inform the engineering of safer, more specific TCR-based therapies that minimise off-target effects.