REMARK 1 REMARK 1 REFERENCE 1 REMARK 1 AUTH A.BONNER,P.B.FURTADO,A.ALMOGREN,M.A.KERR, REMARK 1 AUTH 2 S.J.PERKINS REMARK 1 TITL IMPLICATIONS OF THE NEAR-PLANAR SOLUTION STRUCTURE REMARK 1 TITL 2 OF HUMAN MYELOMA DIMERIC IGA1 FOR MUCOSAL IMMUNITY REMARK 1 TITL 3 AND IGA NEPHROPATHY REMARK 1 REF J.IMMUNOL. V. 180 1008 2008 REMARK 1 REFN ISSN 0022-1767 REMARK 1 PMID 18178841 REMARK 2 REMARK 2 RESOLUTION. NOT APPLICABLE. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : FRODO, INSIGHT REMARK 3 AUTHORS : REMARK 3 REMARK 3 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT. REMARK 3 PROTEIN ATOMS : 7514 REMARK 3 NUCLEIC ACID ATOMS : 0 REMARK 3 HETEROGEN ATOMS : 0 REMARK 3 SOLVENT ATOMS : 0 REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: NULL REMARK 4 REMARK 4 2RCJ COMPLIES WITH FORMAT V. 3.15, 01-DEC-08 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 20-DEC-07. REMARK 100 THE RCSB ID CODE IS RCSB044696. REMARK 265 REMARK 265 EXPERIMENTAL DETAILS REMARK 265 REMARK 265 EXPERIMENT TYPE : SMALL ANGLE X-RAY SCATTERING REMARK 265 DATA ACQUISITION REMARK 265 RADIATION/NEUTRON SOURCE : SRS DARESBURY REMARK 265 SYNCHROTRON (Y/N) : Y REMARK 265 BEAMLINE TYPE : 7.3 REMARK 265 BEAMLINE INSTRUMENT : NULL REMARK 265 DETECTOR TYPE : LINEAR DETECTOR REMARK 265 DETECTOR MANUFACTURER DETAILS : NULL REMARK 265 TEMPERATURE (KELVIN) : 293 REMARK 265 PH : 7.0 REMARK 265 NUMBER OF TIME FRAMES USED : 1 REMARK 265 PROTEIN CONCENTRATION RANGE (MG/ML) : 2-30 REMARK 265 SAMPLE BUFFER : 200 MM NACL 12 MM REMARK 265 NAHPO4 0.05% NA REMARK 265 AZIDE REMARK 265 DATA REDUCTION SOFTWARE : SWANAL REMARK 265 GUINIER MEAN RADIUS OF GYRATION (NM) : 12.17 REMARK 265 SIGMA MEAN RADIUS OF GYRATION : 0.34 REMARK 265 R(XS-1) MEAN CROSS SECTIONAL RADII (NM) : 6.06 REMARK 265 R(XS-1) SIGMA MEAN CROSS SECTIONAL RADII : 0.19 REMARK 265 R(XS-2) MEAN CROSS SECTIONAL RADII (NM) : 1.79 REMARK 265 R(XS-2) SIGMA MEAN CROSS SECTIONAL RADII : 0.12 REMARK 265 P(R) PROTEIN LENGTH (NM) : NULL REMARK 265 REMARK 265 DATA ANALYSIS AND MODEL FITTING: REMARK 265 METHOD USED TO DETERMINE THE STRUCTURE: NULL REMARK 265 SOFTWARE USED : FRODO, INSIGHT, SCTPL2 REMARK 265 SOFTWARE AUTHORS : NULL REMARK 265 STARTING MODEL : NULL REMARK 265 REMARK 265 CONFORMERS, NUMBER CALCULATED : NULL REMARK 265 CONFORMERS, NUMBER SUBMITTED : 1 REMARK 265 CONFORMERS, SELECTION CRITERIA : NULL REMARK 265 REMARK 265 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : NULL REMARK 265 REMARK 265 OTHER DETAILS: THE DEPOSITED STRUCTURE CONTAINS ONLY CA ATOMS. REMARK 265 THE IGM COORDINATES ARE PROVIDED FROM AN EARLY STUDY IN WHICH REMARK 265 A MANUAL FIT METHOD BASED ON KNOWN CRYSTAL STRUCTURES FOR IGG REMARK 265 WAS USED TO DETERMINE STRUCTURES FOR INTACT IGM AND ITS FAB, REMARK 265 FAB'2, FC5 AND IGM-S MONOMER SUBUNITS. THESE CRYSTAL REMARK 265 STRUCTURES ORIGINATED FROM HUMAN FAB KOL (PDB CODE 2FB4) AND REMARK 265 HUMAN FC (PDB CODE 1FC1). THIS PROCEDURE IS APPROXIMATE AND REMARK 265 THE IGM MODEL IS NOT CONSIDERED TO BE OPTIMAL OR REFINED REMARK 265 AGAINST THE X-RAY SCATTERING DATA. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 300 REMARK: THIS STRUCTURE IS A PENTAMER STRUCTURE WITH 4 CHAINS IN REMARK 300 EACH SUBUNIT - PLUS AN ADDITIONAL J CHAIN TO MAKE IT 21 REMARK 300 POLYPEPTIDE CHAINS. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: 21-MERIC REMARK 350 SOFTWARE USED: PISA REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, F, G, H, I, REMARK 350 AND CHAINS: J, K, L, M, N, O, P, Q, R, REMARK 350 AND CHAINS: S, T, U REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT REMARK 500 REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT. REMARK 500 REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE REMARK 500 CA SER P 229 CA LEU P 315 1.40 REMARK 500 CA SER O 231 CA SER O 316 1.60 REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: 1IGA RELATED DB: PDB REMARK 900 HUMAN IMMUNOGLOBULIN A REMARK 900 RELATED ID: 2ESG RELATED DB: PDB REMARK 900 HUMAN MYELOMA IGA1-HUMAN SERUM ALBUMIN COMPLEX REMARK 900 RELATED ID: 2QTJ RELATED DB: PDB REMARK 900 SOLUTION STRUCTURE OF MYELOMA DIMERIC IGA1