HEADER MEMBRANE PROTEIN 14-JUN-23 8PET TITLE CRYO-EM STRUCTURE OF THE FULL-LENGTH HUMAN ALPHA1BETA3 GABA(A) TITLE 2 RECEPTOR (BABBA ARRANGEMENT) IN COMPLEX WITH NANOBODY NB25, EXPRESSED TITLE 3 WITH A 5-FOLD EXCESS OF BETA3 OVER ALPHA1 COMPND MOL_ID: 1; COMPND 2 MOLECULE: GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT ALPHA-1; COMPND 3 CHAIN: A, C; COMPND 4 SYNONYM: GABA(A) RECEPTOR SUBUNIT ALPHA-1; COMPND 5 ENGINEERED: YES; COMPND 6 MOL_ID: 2; COMPND 7 MOLECULE: GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT BETA-3; COMPND 8 CHAIN: B, D, E; COMPND 9 SYNONYM: GABA(A) RECEPTOR SUBUNIT BETA-3,GABAAR SUBUNIT BETA-3; COMPND 10 ENGINEERED: YES; COMPND 11 MOL_ID: 3; COMPND 12 MOLECULE: NANOBODY NB25; COMPND 13 CHAIN: F; COMPND 14 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_COMMON: HUMAN; SOURCE 4 ORGANISM_TAXID: 9606; SOURCE 5 GENE: GABRA1; SOURCE 6 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 8 EXPRESSION_SYSTEM_CELL_LINE: EXPI293; SOURCE 9 MOL_ID: 2; SOURCE 10 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 11 ORGANISM_COMMON: HUMAN; SOURCE 12 ORGANISM_TAXID: 9606; SOURCE 13 GENE: GABRB3; SOURCE 14 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 15 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 16 EXPRESSION_SYSTEM_CELL_LINE: EXPI293; SOURCE 17 MOL_ID: 3; SOURCE 18 ORGANISM_SCIENTIFIC: LAMA GLAMA; SOURCE 19 ORGANISM_TAXID: 9844; SOURCE 20 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 21 EXPRESSION_SYSTEM_TAXID: 562 KEYWDS NEUROTRANSMITTER RECEPTOR, PENTAMERIC LIGAND-GATED ION CHANNEL, TYPE- KEYWDS 2 A GABA RECEPTOR, GABA-A RECEPTOR, MEMBRANE PROTEIN EXPDTA ELECTRON MICROSCOPY AUTHOR A.SENTE,T.MALINAUSKAS,K.NAYDENOVA,S.W.HARDWICK,D.Y.CHIRGADZE, AUTHOR 2 A.R.ARICESCU REVDAT 1 02-JUL-25 8PET 0 JRNL AUTH A.SENTE,T.MALINAUSKAS,K.NAYDENOVA,S.W.HARDWICK, JRNL AUTH 2 D.Y.CHIRGADZE,A.R.ARICESCU JRNL TITL SUBUNIT ABUNDANCE SHAPES GABAA RECEPTOR STOICHIOMETRY JRNL REF TO BE PUBLISHED JRNL REFN REMARK 2 REMARK 2 RESOLUTION. 2.60 ANGSTROMS. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 SOFTWARE PACKAGES : WARP, EPU, CTFFIND, COOT, PHENIX, REMARK 3 CRYOSPARC, CRYOSPARC, CRYOSPARC, REMARK 3 CRYOSPARC REMARK 3 RECONSTRUCTION SCHEMA : NULL REMARK 3 REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT REMARK 3 PDB ENTRY : NULL REMARK 3 REFINEMENT SPACE : NULL REMARK 3 REFINEMENT PROTOCOL : AB INITIO MODEL REMARK 3 REFINEMENT TARGET : NULL REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL REMARK 3 REMARK 3 FITTING PROCEDURE : NULL REMARK 3 REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 2.600 REMARK 3 NUMBER OF PARTICLES : 213401 REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING AND AMPLITUDE REMARK 3 CORRECTION REMARK 3 REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL REMARK 3 REMARK 3 OTHER DETAILS: NULL REMARK 4 REMARK 4 8PET COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 19-JUN-23. REMARK 100 THE DEPOSITION ID IS D_1292131219. REMARK 245 REMARK 245 EXPERIMENTAL DETAILS REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE REMARK 245 SPECIMEN TYPE : NULL REMARK 245 REMARK 245 ELECTRON MICROSCOPE SAMPLE REMARK 245 SAMPLE TYPE : PARTICLE REMARK 245 PARTICLE TYPE : POINT REMARK 245 NAME OF SAMPLE : CRYO-EM STRUCTURE OF THE FULL REMARK 245 -LENGTH HUMAN ALPHA1BETA3 REMARK 245 GABA(A) RECEPTOR (BABBA REMARK 245 ARRANGEMENT) IN COMPLEX WITH REMARK 245 NANOBODY NB25, EXPRESSED WITH A REMARK 245 5-FOLD EXCESS OF BETA3 OVER REMARK 245 ALPHA1 REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : NULL REMARK 245 SAMPLE SUPPORT DETAILS : NULL REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL REMARK 245 SAMPLE BUFFER : NULL REMARK 245 PH : 7.60 REMARK 245 SAMPLE DETAILS : NULL REMARK 245 REMARK 245 DATA ACQUISITION REMARK 245 DATE OF EXPERIMENT : NULL REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : 10020 REMARK 245 TEMPERATURE (KELVIN) : NULL REMARK 245 MICROSCOPE MODEL : FEI TITAN KRIOS REMARK 245 DETECTOR TYPE : GATAN K3 (6K X 4K) REMARK 245 MINIMUM DEFOCUS (NM) : 700.00 REMARK 245 MAXIMUM DEFOCUS (NM) : 2100.00 REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 NOMINAL CS : 2.70 REMARK 245 IMAGING MODE : BRIGHT FIELD REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 5063.00 REMARK 245 ILLUMINATION MODE : FLOOD BEAM REMARK 245 NOMINAL MAGNIFICATION : 130000 REMARK 245 CALIBRATED MAGNIFICATION : NULL REMARK 245 SOURCE : FIELD EMISSION GUN REMARK 245 ACCELERATION VOLTAGE (KV) : 300 REMARK 245 IMAGING DETAILS : NULL REMARK 247 REMARK 247 ELECTRON MICROSCOPY REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION REMARK 247 OF THE STRUCTURE FACTORS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: HEXAMERIC REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: HEXAMERIC REMARK 350 SOFTWARE USED: PISA REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, D, C, E, F, a, b, G, c, REMARK 350 AND CHAINS: H, d, e, I REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 REMARK 465 M RES C SSSEQI REMARK 465 MET A -54 REMARK 465 GLY A -53 REMARK 465 ILE A -52 REMARK 465 LEU A -51 REMARK 465 PRO A -50 REMARK 465 SER A -49 REMARK 465 PRO A -48 REMARK 465 GLY A -47 REMARK 465 MET A -46 REMARK 465 PRO A -45 REMARK 465 ALA A -44 REMARK 465 LEU A -43 REMARK 465 LEU A -42 REMARK 465 SER A -41 REMARK 465 LEU A -40 REMARK 465 VAL A -39 REMARK 465 SER A -38 REMARK 465 LEU A -37 REMARK 465 LEU A -36 REMARK 465 SER A -35 REMARK 465 VAL A -34 REMARK 465 LEU A -33 REMARK 465 LEU A -32 REMARK 465 MET A -31 REMARK 465 GLY A -30 REMARK 465 CYS A -29 REMARK 465 VAL A -28 REMARK 465 ALA A -27 REMARK 465 GLU A -26 REMARK 465 THR A -25 REMARK 465 GLY A -24 REMARK 465 TRP A -23 REMARK 465 SER A -22 REMARK 465 HIS A -21 REMARK 465 PRO A -20 REMARK 465 GLN A -19 REMARK 465 PHE A -18 REMARK 465 GLU A -17 REMARK 465 LYS A -16 REMARK 465 GLY A -15 REMARK 465 GLY A -14 REMARK 465 GLY A -13 REMARK 465 SER A -12 REMARK 465 GLY A -11 REMARK 465 GLY A -10 REMARK 465 GLY A -9 REMARK 465 SER A -8 REMARK 465 GLY A -7 REMARK 465 GLY A -6 REMARK 465 SER A -5 REMARK 465 ALA A -4 REMARK 465 TRP A -3 REMARK 465 SER A -2 REMARK 465 HIS A -1 REMARK 465 PRO A 0 REMARK 465 GLN A 1 REMARK 465 PHE A 2 REMARK 465 GLU A 3 REMARK 465 LYS A 4 REMARK 465 GLY A 5 REMARK 465 GLY A 6 REMARK 465 SER A 7 REMARK 465 THR A 8 REMARK 465 GLY A 9 REMARK 465 ASP A 10 REMARK 465 ASN A 11 REMARK 465 VAL A 322 REMARK 465 VAL A 323 REMARK 465 PRO A 324 REMARK 465 GLU A 325 REMARK 465 LYS A 326 REMARK 465 PRO A 327 REMARK 465 LYS A 328 REMARK 465 LYS A 329 REMARK 465 VAL A 330 REMARK 465 LYS A 331 REMARK 465 ASP A 332 REMARK 465 PRO A 333 REMARK 465 LEU A 334 REMARK 465 ILE A 335 REMARK 465 LYS A 336 REMARK 465 LYS A 337 REMARK 465 ASN A 338 REMARK 465 ASN A 339 REMARK 465 THR A 340 REMARK 465 TYR A 341 REMARK 465 ALA A 342 REMARK 465 PRO A 343 REMARK 465 THR A 344 REMARK 465 ALA A 345 REMARK 465 THR A 346 REMARK 465 SER A 347 REMARK 465 TYR A 348 REMARK 465 THR A 349 REMARK 465 PRO A 350 REMARK 465 ASN A 351 REMARK 465 LEU A 352 REMARK 465 ALA A 353 REMARK 465 ARG A 354 REMARK 465 GLY A 355 REMARK 465 ASP A 356 REMARK 465 PRO A 357 REMARK 465 GLY A 358 REMARK 465 LEU A 359 REMARK 465 ALA A 360 REMARK 465 THR A 361 REMARK 465 ILE A 362 REMARK 465 ALA A 363 REMARK 465 LYS A 364 REMARK 465 SER A 365 REMARK 465 ALA A 366 REMARK 465 THR A 367 REMARK 465 ILE A 368 REMARK 465 GLU A 369 REMARK 465 PRO A 370 REMARK 465 LYS A 371 REMARK 465 GLU A 372 REMARK 465 VAL A 373 REMARK 465 LYS A 374 REMARK 465 PRO A 375 REMARK 465 GLU A 376 REMARK 465 THR A 377 REMARK 465 LYS A 378 REMARK 465 PRO A 379 REMARK 465 PRO A 380 REMARK 465 GLU A 381 REMARK 465 PRO A 382 REMARK 465 LYS A 383 REMARK 465 ASN A 417 REMARK 465 ARG A 418 REMARK 465 GLU A 419 REMARK 465 PRO A 420 REMARK 465 GLN A 421 REMARK 465 LEU A 422 REMARK 465 LYS A 423 REMARK 465 ALA A 424 REMARK 465 PRO A 425 REMARK 465 THR A 426 REMARK 465 PRO A 427 REMARK 465 HIS A 428 REMARK 465 GLN A 429 REMARK 465 MET B -39 REMARK 465 GLY B -38 REMARK 465 ILE B -37 REMARK 465 LEU B -36 REMARK 465 PRO B -35 REMARK 465 SER B -34 REMARK 465 PRO B -33 REMARK 465 GLY B -32 REMARK 465 MET B -31 REMARK 465 PRO B -30 REMARK 465 ALA B -29 REMARK 465 LEU B -28 REMARK 465 LEU B -27 REMARK 465 SER B -26 REMARK 465 LEU B -25 REMARK 465 VAL B -24 REMARK 465 SER B -23 REMARK 465 LEU B -22 REMARK 465 LEU B -21 REMARK 465 SER B -20 REMARK 465 VAL B -19 REMARK 465 LEU B -18 REMARK 465 LEU B -17 REMARK 465 MET B -16 REMARK 465 GLY B -15 REMARK 465 CYS B -14 REMARK 465 VAL B -13 REMARK 465 ALA B -12 REMARK 465 GLU B -11 REMARK 465 TYR B -10 REMARK 465 PRO B -9 REMARK 465 TYR B -8 REMARK 465 ASP B -7 REMARK 465 VAL B -6 REMARK 465 PRO B -5 REMARK 465 ASP B -4 REMARK 465 TYR B -3 REMARK 465 ALA B -2 REMARK 465 THR B -1 REMARK 465 GLY B 0 REMARK 465 GLN B 1 REMARK 465 SER B 2 REMARK 465 VAL B 3 REMARK 465 ASN B 4 REMARK 465 ASP B 5 REMARK 465 PRO B 6 REMARK 465 GLY B 7 REMARK 465 ASN B 8 REMARK 465 GLN B 335 REMARK 465 ARG B 336 REMARK 465 GLN B 337 REMARK 465 LYS B 338 REMARK 465 LYS B 339 REMARK 465 LEU B 340 REMARK 465 ALA B 341 REMARK 465 GLU B 342 REMARK 465 LYS B 343 REMARK 465 THR B 344 REMARK 465 ALA B 345 REMARK 465 LYS B 346 REMARK 465 ALA B 347 REMARK 465 LYS B 348 REMARK 465 ASN B 349 REMARK 465 ASP B 350 REMARK 465 ARG B 351 REMARK 465 SER B 352 REMARK 465 LYS B 353 REMARK 465 SER B 354 REMARK 465 GLU B 355 REMARK 465 SER B 356 REMARK 465 ASN B 357 REMARK 465 ARG B 358 REMARK 465 VAL B 359 REMARK 465 ASP B 360 REMARK 465 ALA B 361 REMARK 465 HIS B 362 REMARK 465 GLY B 363 REMARK 465 ASN B 364 REMARK 465 ILE B 365 REMARK 465 LEU B 366 REMARK 465 LEU B 367 REMARK 465 THR B 368 REMARK 465 SER B 369 REMARK 465 LEU B 370 REMARK 465 GLU B 371 REMARK 465 VAL B 372 REMARK 465 HIS B 373 REMARK 465 ASN B 374 REMARK 465 GLU B 375 REMARK 465 MET B 376 REMARK 465 ASN B 377 REMARK 465 GLU B 378 REMARK 465 VAL B 379 REMARK 465 SER B 380 REMARK 465 GLY B 381 REMARK 465 GLY B 382 REMARK 465 ILE B 383 REMARK 465 GLY B 384 REMARK 465 ASP B 385 REMARK 465 THR B 386 REMARK 465 ARG B 387 REMARK 465 ASN B 388 REMARK 465 SER B 389 REMARK 465 ALA B 390 REMARK 465 ILE B 391 REMARK 465 SER B 392 REMARK 465 PHE B 393 REMARK 465 ASP B 394 REMARK 465 ASN B 395 REMARK 465 SER B 396 REMARK 465 GLY B 397 REMARK 465 ILE B 398 REMARK 465 GLN B 399 REMARK 465 TYR B 400 REMARK 465 ARG B 401 REMARK 465 LYS B 402 REMARK 465 GLN B 403 REMARK 465 SER B 404 REMARK 465 MET B 405 REMARK 465 PRO B 406 REMARK 465 ARG B 407 REMARK 465 GLU B 408 REMARK 465 GLY B 409 REMARK 465 HIS B 410 REMARK 465 GLY B 411 REMARK 465 ARG B 412 REMARK 465 PHE B 413 REMARK 465 LEU B 414 REMARK 465 GLY B 415 REMARK 465 ASP B 416 REMARK 465 ARG B 417 REMARK 465 SER B 418 REMARK 465 LEU B 419 REMARK 465 PRO B 420 REMARK 465 HIS B 421 REMARK 465 LYS B 422 REMARK 465 LYS B 423 REMARK 465 GLY B 424 REMARK 465 THR B 425 REMARK 465 THR B 426 REMARK 465 HIS B 427 REMARK 465 LEU B 428 REMARK 465 ARG B 429 REMARK 465 ARG B 430 REMARK 465 ARG B 431 REMARK 465 SER B 432 REMARK 465 SER B 433 REMARK 465 GLN B 434 REMARK 465 LEU B 435 REMARK 465 LYS B 436 REMARK 465 ILE B 437 REMARK 465 LYS B 438 REMARK 465 ILE B 439 REMARK 465 PRO B 440 REMARK 465 ASP B 441 REMARK 465 LEU B 442 REMARK 465 THR B 443 REMARK 465 ASN B 473 REMARK 465 MET D -39 REMARK 465 GLY D -38 REMARK 465 ILE D -37 REMARK 465 LEU D -36 REMARK 465 PRO D -35 REMARK 465 SER D -34 REMARK 465 PRO D -33 REMARK 465 GLY D -32 REMARK 465 MET D -31 REMARK 465 PRO D -30 REMARK 465 ALA D -29 REMARK 465 LEU D -28 REMARK 465 LEU D -27 REMARK 465 SER D -26 REMARK 465 LEU D -25 REMARK 465 VAL D -24 REMARK 465 SER D -23 REMARK 465 LEU D -22 REMARK 465 LEU D -21 REMARK 465 SER D -20 REMARK 465 VAL D -19 REMARK 465 LEU D -18 REMARK 465 LEU D -17 REMARK 465 MET D -16 REMARK 465 GLY D -15 REMARK 465 CYS D -14 REMARK 465 VAL D -13 REMARK 465 ALA D -12 REMARK 465 GLU D -11 REMARK 465 TYR D -10 REMARK 465 PRO D -9 REMARK 465 TYR D -8 REMARK 465 ASP D -7 REMARK 465 VAL D -6 REMARK 465 PRO D -5 REMARK 465 ASP D -4 REMARK 465 TYR D -3 REMARK 465 ALA D -2 REMARK 465 THR D -1 REMARK 465 GLY D 0 REMARK 465 GLN D 1 REMARK 465 SER D 2 REMARK 465 VAL D 3 REMARK 465 ASN D 4 REMARK 465 ASP D 5 REMARK 465 PRO D 6 REMARK 465 GLY D 7 REMARK 465 ASN D 8 REMARK 465 GLY D 333 REMARK 465 PRO D 334 REMARK 465 GLN D 335 REMARK 465 ARG D 336 REMARK 465 GLN D 337 REMARK 465 LYS D 338 REMARK 465 LYS D 339 REMARK 465 LEU D 340 REMARK 465 ALA D 341 REMARK 465 GLU D 342 REMARK 465 LYS D 343 REMARK 465 THR D 344 REMARK 465 ALA D 345 REMARK 465 LYS D 346 REMARK 465 ALA D 347 REMARK 465 LYS D 348 REMARK 465 ASN D 349 REMARK 465 ASP D 350 REMARK 465 ARG D 351 REMARK 465 SER D 352 REMARK 465 LYS D 353 REMARK 465 SER D 354 REMARK 465 GLU D 355 REMARK 465 SER D 356 REMARK 465 ASN D 357 REMARK 465 ARG D 358 REMARK 465 VAL D 359 REMARK 465 ASP D 360 REMARK 465 ALA D 361 REMARK 465 HIS D 362 REMARK 465 GLY D 363 REMARK 465 ASN D 364 REMARK 465 ILE D 365 REMARK 465 LEU D 366 REMARK 465 LEU D 367 REMARK 465 THR D 368 REMARK 465 SER D 369 REMARK 465 LEU D 370 REMARK 465 GLU D 371 REMARK 465 VAL D 372 REMARK 465 HIS D 373 REMARK 465 ASN D 374 REMARK 465 GLU D 375 REMARK 465 MET D 376 REMARK 465 ASN D 377 REMARK 465 GLU D 378 REMARK 465 VAL D 379 REMARK 465 SER D 380 REMARK 465 GLY D 381 REMARK 465 GLY D 382 REMARK 465 ILE D 383 REMARK 465 GLY D 384 REMARK 465 ASP D 385 REMARK 465 THR D 386 REMARK 465 ARG D 387 REMARK 465 ASN D 388 REMARK 465 SER D 389 REMARK 465 ALA D 390 REMARK 465 ILE D 391 REMARK 465 SER D 392 REMARK 465 PHE D 393 REMARK 465 ASP D 394 REMARK 465 ASN D 395 REMARK 465 SER D 396 REMARK 465 GLY D 397 REMARK 465 ILE D 398 REMARK 465 GLN D 399 REMARK 465 TYR D 400 REMARK 465 ARG D 401 REMARK 465 LYS D 402 REMARK 465 GLN D 403 REMARK 465 SER D 404 REMARK 465 MET D 405 REMARK 465 PRO D 406 REMARK 465 ARG D 407 REMARK 465 GLU D 408 REMARK 465 GLY D 409 REMARK 465 HIS D 410 REMARK 465 GLY D 411 REMARK 465 ARG D 412 REMARK 465 PHE D 413 REMARK 465 LEU D 414 REMARK 465 GLY D 415 REMARK 465 ASP D 416 REMARK 465 ARG D 417 REMARK 465 SER D 418 REMARK 465 LEU D 419 REMARK 465 PRO D 420 REMARK 465 HIS D 421 REMARK 465 LYS D 422 REMARK 465 LYS D 423 REMARK 465 GLY D 424 REMARK 465 THR D 425 REMARK 465 THR D 426 REMARK 465 HIS D 427 REMARK 465 LEU D 428 REMARK 465 ARG D 429 REMARK 465 ARG D 430 REMARK 465 ARG D 431 REMARK 465 SER D 432 REMARK 465 SER D 433 REMARK 465 GLN D 434 REMARK 465 LEU D 435 REMARK 465 LYS D 436 REMARK 465 ILE D 437 REMARK 465 LYS D 438 REMARK 465 ILE D 439 REMARK 465 PRO D 440 REMARK 465 ASP D 441 REMARK 465 LEU D 442 REMARK 465 THR D 443 REMARK 465 ASN D 473 REMARK 465 MET C -54 REMARK 465 GLY C -53 REMARK 465 ILE C -52 REMARK 465 LEU C -51 REMARK 465 PRO C -50 REMARK 465 SER C -49 REMARK 465 PRO C -48 REMARK 465 GLY C -47 REMARK 465 MET C -46 REMARK 465 PRO C -45 REMARK 465 ALA C -44 REMARK 465 LEU C -43 REMARK 465 LEU C -42 REMARK 465 SER C -41 REMARK 465 LEU C -40 REMARK 465 VAL C -39 REMARK 465 SER C -38 REMARK 465 LEU C -37 REMARK 465 LEU C -36 REMARK 465 SER C -35 REMARK 465 VAL C -34 REMARK 465 LEU C -33 REMARK 465 LEU C -32 REMARK 465 MET C -31 REMARK 465 GLY C -30 REMARK 465 CYS C -29 REMARK 465 VAL C -28 REMARK 465 ALA C -27 REMARK 465 GLU C -26 REMARK 465 THR C -25 REMARK 465 GLY C -24 REMARK 465 TRP C -23 REMARK 465 SER C -22 REMARK 465 HIS C -21 REMARK 465 PRO C -20 REMARK 465 GLN C -19 REMARK 465 PHE C -18 REMARK 465 GLU C -17 REMARK 465 LYS C -16 REMARK 465 GLY C -15 REMARK 465 GLY C -14 REMARK 465 GLY C -13 REMARK 465 SER C -12 REMARK 465 GLY C -11 REMARK 465 GLY C -10 REMARK 465 GLY C -9 REMARK 465 SER C -8 REMARK 465 GLY C -7 REMARK 465 GLY C -6 REMARK 465 SER C -5 REMARK 465 ALA C -4 REMARK 465 TRP C -3 REMARK 465 SER C -2 REMARK 465 HIS C -1 REMARK 465 PRO C 0 REMARK 465 GLN C 1 REMARK 465 PHE C 2 REMARK 465 GLU C 3 REMARK 465 LYS C 4 REMARK 465 GLY C 5 REMARK 465 GLY C 6 REMARK 465 SER C 7 REMARK 465 THR C 8 REMARK 465 GLY C 9 REMARK 465 ASP C 10 REMARK 465 ASN C 11 REMARK 465 VAL C 322 REMARK 465 VAL C 323 REMARK 465 PRO C 324 REMARK 465 GLU C 325 REMARK 465 LYS C 326 REMARK 465 PRO C 327 REMARK 465 LYS C 328 REMARK 465 LYS C 329 REMARK 465 VAL C 330 REMARK 465 LYS C 331 REMARK 465 ASP C 332 REMARK 465 PRO C 333 REMARK 465 LEU C 334 REMARK 465 ILE C 335 REMARK 465 LYS C 336 REMARK 465 LYS C 337 REMARK 465 ASN C 338 REMARK 465 ASN C 339 REMARK 465 THR C 340 REMARK 465 TYR C 341 REMARK 465 ALA C 342 REMARK 465 PRO C 343 REMARK 465 THR C 344 REMARK 465 ALA C 345 REMARK 465 THR C 346 REMARK 465 SER C 347 REMARK 465 TYR C 348 REMARK 465 THR C 349 REMARK 465 PRO C 350 REMARK 465 ASN C 351 REMARK 465 LEU C 352 REMARK 465 ALA C 353 REMARK 465 ARG C 354 REMARK 465 GLY C 355 REMARK 465 ASP C 356 REMARK 465 PRO C 357 REMARK 465 GLY C 358 REMARK 465 LEU C 359 REMARK 465 ALA C 360 REMARK 465 THR C 361 REMARK 465 ILE C 362 REMARK 465 ALA C 363 REMARK 465 LYS C 364 REMARK 465 SER C 365 REMARK 465 ALA C 366 REMARK 465 THR C 367 REMARK 465 ILE C 368 REMARK 465 GLU C 369 REMARK 465 PRO C 370 REMARK 465 LYS C 371 REMARK 465 GLU C 372 REMARK 465 VAL C 373 REMARK 465 LYS C 374 REMARK 465 PRO C 375 REMARK 465 GLU C 376 REMARK 465 THR C 377 REMARK 465 LYS C 378 REMARK 465 PRO C 379 REMARK 465 PRO C 380 REMARK 465 GLU C 381 REMARK 465 PRO C 382 REMARK 465 LYS C 383 REMARK 465 ASN C 417 REMARK 465 ARG C 418 REMARK 465 GLU C 419 REMARK 465 PRO C 420 REMARK 465 GLN C 421 REMARK 465 LEU C 422 REMARK 465 LYS C 423 REMARK 465 ALA C 424 REMARK 465 PRO C 425 REMARK 465 THR C 426 REMARK 465 PRO C 427 REMARK 465 HIS C 428 REMARK 465 GLN C 429 REMARK 465 MET E -39 REMARK 465 GLY E -38 REMARK 465 ILE E -37 REMARK 465 LEU E -36 REMARK 465 PRO E -35 REMARK 465 SER E -34 REMARK 465 PRO E -33 REMARK 465 GLY E -32 REMARK 465 MET E -31 REMARK 465 PRO E -30 REMARK 465 ALA E -29 REMARK 465 LEU E -28 REMARK 465 LEU E -27 REMARK 465 SER E -26 REMARK 465 LEU E -25 REMARK 465 VAL E -24 REMARK 465 SER E -23 REMARK 465 LEU E -22 REMARK 465 LEU E -21 REMARK 465 SER E -20 REMARK 465 VAL E -19 REMARK 465 LEU E -18 REMARK 465 LEU E -17 REMARK 465 MET E -16 REMARK 465 GLY E -15 REMARK 465 CYS E -14 REMARK 465 VAL E -13 REMARK 465 ALA E -12 REMARK 465 GLU E -11 REMARK 465 TYR E -10 REMARK 465 PRO E -9 REMARK 465 TYR E -8 REMARK 465 ASP E -7 REMARK 465 VAL E -6 REMARK 465 PRO E -5 REMARK 465 ASP E -4 REMARK 465 TYR E -3 REMARK 465 ALA E -2 REMARK 465 THR E -1 REMARK 465 GLY E 0 REMARK 465 GLN E 1 REMARK 465 SER E 2 REMARK 465 VAL E 3 REMARK 465 ASN E 4 REMARK 465 ASP E 5 REMARK 465 PRO E 6 REMARK 465 GLY E 7 REMARK 465 ASN E 8 REMARK 465 GLY E 333 REMARK 465 PRO E 334 REMARK 465 GLN E 335 REMARK 465 ARG E 336 REMARK 465 GLN E 337 REMARK 465 LYS E 338 REMARK 465 LYS E 339 REMARK 465 LEU E 340 REMARK 465 ALA E 341 REMARK 465 GLU E 342 REMARK 465 LYS E 343 REMARK 465 THR E 344 REMARK 465 ALA E 345 REMARK 465 LYS E 346 REMARK 465 ALA E 347 REMARK 465 LYS E 348 REMARK 465 ASN E 349 REMARK 465 ASP E 350 REMARK 465 ARG E 351 REMARK 465 SER E 352 REMARK 465 LYS E 353 REMARK 465 SER E 354 REMARK 465 GLU E 355 REMARK 465 SER E 356 REMARK 465 ASN E 357 REMARK 465 ARG E 358 REMARK 465 VAL E 359 REMARK 465 ASP E 360 REMARK 465 ALA E 361 REMARK 465 HIS E 362 REMARK 465 GLY E 363 REMARK 465 ASN E 364 REMARK 465 ILE E 365 REMARK 465 LEU E 366 REMARK 465 LEU E 367 REMARK 465 THR E 368 REMARK 465 SER E 369 REMARK 465 LEU E 370 REMARK 465 GLU E 371 REMARK 465 VAL E 372 REMARK 465 HIS E 373 REMARK 465 ASN E 374 REMARK 465 GLU E 375 REMARK 465 MET E 376 REMARK 465 ASN E 377 REMARK 465 GLU E 378 REMARK 465 VAL E 379 REMARK 465 SER E 380 REMARK 465 GLY E 381 REMARK 465 GLY E 382 REMARK 465 ILE E 383 REMARK 465 GLY E 384 REMARK 465 ASP E 385 REMARK 465 THR E 386 REMARK 465 ARG E 387 REMARK 465 ASN E 388 REMARK 465 SER E 389 REMARK 465 ALA E 390 REMARK 465 ILE E 391 REMARK 465 SER E 392 REMARK 465 PHE E 393 REMARK 465 ASP E 394 REMARK 465 ASN E 395 REMARK 465 SER E 396 REMARK 465 GLY E 397 REMARK 465 ILE E 398 REMARK 465 GLN E 399 REMARK 465 TYR E 400 REMARK 465 ARG E 401 REMARK 465 LYS E 402 REMARK 465 GLN E 403 REMARK 465 SER E 404 REMARK 465 MET E 405 REMARK 465 PRO E 406 REMARK 465 ARG E 407 REMARK 465 GLU E 408 REMARK 465 GLY E 409 REMARK 465 HIS E 410 REMARK 465 GLY E 411 REMARK 465 ARG E 412 REMARK 465 PHE E 413 REMARK 465 LEU E 414 REMARK 465 GLY E 415 REMARK 465 ASP E 416 REMARK 465 ARG E 417 REMARK 465 SER E 418 REMARK 465 LEU E 419 REMARK 465 PRO E 420 REMARK 465 HIS E 421 REMARK 465 LYS E 422 REMARK 465 LYS E 423 REMARK 465 GLY E 424 REMARK 465 THR E 425 REMARK 465 THR E 426 REMARK 465 HIS E 427 REMARK 465 LEU E 428 REMARK 465 ARG E 429 REMARK 465 ARG E 430 REMARK 465 ARG E 431 REMARK 465 SER E 432 REMARK 465 SER E 433 REMARK 465 GLN E 434 REMARK 465 LEU E 435 REMARK 465 LYS E 436 REMARK 465 ILE E 437 REMARK 465 LYS E 438 REMARK 465 ILE E 439 REMARK 465 PRO E 440 REMARK 465 ASP E 441 REMARK 465 LEU E 442 REMARK 465 THR E 443 REMARK 465 ASN E 473 REMARK 465 SER F 125 REMARK 465 HIS F 126 REMARK 465 HIS F 127 REMARK 465 HIS F 128 REMARK 465 HIS F 129 REMARK 465 HIS F 130 REMARK 465 HIS F 131 REMARK 465 GLU F 132 REMARK 465 PRO F 133 REMARK 465 GLU F 134 REMARK 465 ALA F 135 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 SER A 49 117.32 -161.72 REMARK 500 ASP A 98 59.13 -91.62 REMARK 500 LEU A 143 31.32 -98.68 REMARK 500 MET A 148 41.49 -107.73 REMARK 500 ARG A 173 -166.20 -127.48 REMARK 500 ASP A 184 31.42 -98.42 REMARK 500 ARG A 187 41.11 -109.24 REMARK 500 PRO B 78 46.33 -81.32 REMARK 500 LEU B 145 32.18 -97.43 REMARK 500 LEU B 268 43.73 -89.29 REMARK 500 PRO B 273 47.78 -79.80 REMARK 500 LYS B 274 65.66 36.79 REMARK 500 PRO D 78 46.56 -83.69 REMARK 500 ASP D 95 53.40 -90.17 REMARK 500 LEU D 145 30.50 -92.43 REMARK 500 PRO D 273 48.67 -81.35 REMARK 500 PHE D 306 -61.37 -92.87 REMARK 500 ASP C 98 58.90 -90.20 REMARK 500 LYS C 106 114.68 -161.24 REMARK 500 ARG C 173 -167.55 -126.99 REMARK 500 ARG C 187 42.07 -109.57 REMARK 500 PRO E 78 48.09 -77.65 REMARK 500 PHE E 306 -62.08 -94.51 REMARK 500 REMARK 500 REMARK: NULL REMARK 620 REMARK 620 METAL COORDINATION REMARK 620 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 620 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE): REMARK 620 REMARK 620 COORDINATION ANGLES FOR: M RES CSSEQI METAL REMARK 620 ZN B 505 ZN REMARK 620 N RES CSSEQI ATOM REMARK 620 1 HIS B 267 NE2 REMARK 620 2 HIS D 267 NE2 85.9 REMARK 620 3 HIS E 267 ND1 142.0 75.4 REMARK 620 N 1 2 REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: EMD-17636 RELATED DB: EMDB REMARK 900 CRYO-EM STRUCTURE OF HUMAN FULL-LENGTH ALPHA1BETA3 GABA(A)R (BABBA REMARK 900 ARRANGEMENT) IN COMPLEX WITH NANOBODY NB25, TRANSFECTED WITH A 5- REMARK 900 FOLD EXCESS OF BETA3 DBREF 8PET A 10 429 UNP P14867 GBRA1_HUMAN 37 456 DBREF 8PET B 1 473 UNP P28472 GBRB3_HUMAN 26 473 DBREF 8PET D 1 473 UNP P28472 GBRB3_HUMAN 26 473 DBREF 8PET C 10 429 UNP P14867 GBRA1_HUMAN 37 456 DBREF 8PET E 1 473 UNP P28472 GBRB3_HUMAN 26 473 DBREF 8PET F 1 135 PDB 8PET 8PET 1 135 SEQADV 8PET MET A -54 UNP P14867 INITIATING METHIONINE SEQADV 8PET GLY A -53 UNP P14867 EXPRESSION TAG SEQADV 8PET ILE A -52 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU A -51 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO A -50 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -49 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO A -48 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -47 UNP P14867 EXPRESSION TAG SEQADV 8PET MET A -46 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO A -45 UNP P14867 EXPRESSION TAG SEQADV 8PET ALA A -44 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU A -43 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU A -42 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -41 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU A -40 UNP P14867 EXPRESSION TAG SEQADV 8PET VAL A -39 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -38 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU A -37 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU A -36 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -35 UNP P14867 EXPRESSION TAG SEQADV 8PET VAL A -34 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU A -33 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU A -32 UNP P14867 EXPRESSION TAG SEQADV 8PET MET A -31 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -30 UNP P14867 EXPRESSION TAG SEQADV 8PET CYS A -29 UNP P14867 EXPRESSION TAG SEQADV 8PET VAL A -28 UNP P14867 EXPRESSION TAG SEQADV 8PET ALA A -27 UNP P14867 EXPRESSION TAG SEQADV 8PET GLU A -26 UNP P14867 EXPRESSION TAG SEQADV 8PET THR A -25 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -24 UNP P14867 EXPRESSION TAG SEQADV 8PET TRP A -23 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -22 UNP P14867 EXPRESSION TAG SEQADV 8PET HIS A -21 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO A -20 UNP P14867 EXPRESSION TAG SEQADV 8PET GLN A -19 UNP P14867 EXPRESSION TAG SEQADV 8PET PHE A -18 UNP P14867 EXPRESSION TAG SEQADV 8PET GLU A -17 UNP P14867 EXPRESSION TAG SEQADV 8PET LYS A -16 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -15 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -14 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -13 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -12 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -11 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -10 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -9 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -8 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -7 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A -6 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -5 UNP P14867 EXPRESSION TAG SEQADV 8PET ALA A -4 UNP P14867 EXPRESSION TAG SEQADV 8PET TRP A -3 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A -2 UNP P14867 EXPRESSION TAG SEQADV 8PET HIS A -1 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO A 0 UNP P14867 EXPRESSION TAG SEQADV 8PET GLN A 1 UNP P14867 EXPRESSION TAG SEQADV 8PET PHE A 2 UNP P14867 EXPRESSION TAG SEQADV 8PET GLU A 3 UNP P14867 EXPRESSION TAG SEQADV 8PET LYS A 4 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A 5 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A 6 UNP P14867 EXPRESSION TAG SEQADV 8PET SER A 7 UNP P14867 EXPRESSION TAG SEQADV 8PET THR A 8 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY A 9 UNP P14867 EXPRESSION TAG SEQADV 8PET MET B -39 UNP P28472 INITIATING METHIONINE SEQADV 8PET GLY B -38 UNP P28472 EXPRESSION TAG SEQADV 8PET ILE B -37 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU B -36 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO B -35 UNP P28472 EXPRESSION TAG SEQADV 8PET SER B -34 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO B -33 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY B -32 UNP P28472 EXPRESSION TAG SEQADV 8PET MET B -31 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO B -30 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA B -29 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU B -28 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU B -27 UNP P28472 EXPRESSION TAG SEQADV 8PET SER B -26 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU B -25 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL B -24 UNP P28472 EXPRESSION TAG SEQADV 8PET SER B -23 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU B -22 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU B -21 UNP P28472 EXPRESSION TAG SEQADV 8PET SER B -20 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL B -19 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU B -18 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU B -17 UNP P28472 EXPRESSION TAG SEQADV 8PET MET B -16 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY B -15 UNP P28472 EXPRESSION TAG SEQADV 8PET CYS B -14 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL B -13 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA B -12 UNP P28472 EXPRESSION TAG SEQADV 8PET GLU B -11 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR B -10 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO B -9 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR B -8 UNP P28472 EXPRESSION TAG SEQADV 8PET ASP B -7 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL B -6 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO B -5 UNP P28472 EXPRESSION TAG SEQADV 8PET ASP B -4 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR B -3 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA B -2 UNP P28472 EXPRESSION TAG SEQADV 8PET THR B -1 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY B 0 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY B 424 UNP P28472 INSERTION SEQADV 8PET THR B 425 UNP P28472 INSERTION SEQADV 8PET MET D -39 UNP P28472 INITIATING METHIONINE SEQADV 8PET GLY D -38 UNP P28472 EXPRESSION TAG SEQADV 8PET ILE D -37 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU D -36 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO D -35 UNP P28472 EXPRESSION TAG SEQADV 8PET SER D -34 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO D -33 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY D -32 UNP P28472 EXPRESSION TAG SEQADV 8PET MET D -31 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO D -30 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA D -29 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU D -28 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU D -27 UNP P28472 EXPRESSION TAG SEQADV 8PET SER D -26 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU D -25 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL D -24 UNP P28472 EXPRESSION TAG SEQADV 8PET SER D -23 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU D -22 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU D -21 UNP P28472 EXPRESSION TAG SEQADV 8PET SER D -20 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL D -19 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU D -18 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU D -17 UNP P28472 EXPRESSION TAG SEQADV 8PET MET D -16 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY D -15 UNP P28472 EXPRESSION TAG SEQADV 8PET CYS D -14 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL D -13 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA D -12 UNP P28472 EXPRESSION TAG SEQADV 8PET GLU D -11 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR D -10 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO D -9 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR D -8 UNP P28472 EXPRESSION TAG SEQADV 8PET ASP D -7 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL D -6 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO D -5 UNP P28472 EXPRESSION TAG SEQADV 8PET ASP D -4 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR D -3 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA D -2 UNP P28472 EXPRESSION TAG SEQADV 8PET THR D -1 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY D 0 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY D 424 UNP P28472 INSERTION SEQADV 8PET THR D 425 UNP P28472 INSERTION SEQADV 8PET MET C -54 UNP P14867 INITIATING METHIONINE SEQADV 8PET GLY C -53 UNP P14867 EXPRESSION TAG SEQADV 8PET ILE C -52 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU C -51 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO C -50 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -49 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO C -48 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -47 UNP P14867 EXPRESSION TAG SEQADV 8PET MET C -46 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO C -45 UNP P14867 EXPRESSION TAG SEQADV 8PET ALA C -44 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU C -43 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU C -42 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -41 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU C -40 UNP P14867 EXPRESSION TAG SEQADV 8PET VAL C -39 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -38 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU C -37 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU C -36 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -35 UNP P14867 EXPRESSION TAG SEQADV 8PET VAL C -34 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU C -33 UNP P14867 EXPRESSION TAG SEQADV 8PET LEU C -32 UNP P14867 EXPRESSION TAG SEQADV 8PET MET C -31 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -30 UNP P14867 EXPRESSION TAG SEQADV 8PET CYS C -29 UNP P14867 EXPRESSION TAG SEQADV 8PET VAL C -28 UNP P14867 EXPRESSION TAG SEQADV 8PET ALA C -27 UNP P14867 EXPRESSION TAG SEQADV 8PET GLU C -26 UNP P14867 EXPRESSION TAG SEQADV 8PET THR C -25 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -24 UNP P14867 EXPRESSION TAG SEQADV 8PET TRP C -23 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -22 UNP P14867 EXPRESSION TAG SEQADV 8PET HIS C -21 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO C -20 UNP P14867 EXPRESSION TAG SEQADV 8PET GLN C -19 UNP P14867 EXPRESSION TAG SEQADV 8PET PHE C -18 UNP P14867 EXPRESSION TAG SEQADV 8PET GLU C -17 UNP P14867 EXPRESSION TAG SEQADV 8PET LYS C -16 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -15 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -14 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -13 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -12 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -11 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -10 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -9 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -8 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -7 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C -6 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -5 UNP P14867 EXPRESSION TAG SEQADV 8PET ALA C -4 UNP P14867 EXPRESSION TAG SEQADV 8PET TRP C -3 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C -2 UNP P14867 EXPRESSION TAG SEQADV 8PET HIS C -1 UNP P14867 EXPRESSION TAG SEQADV 8PET PRO C 0 UNP P14867 EXPRESSION TAG SEQADV 8PET GLN C 1 UNP P14867 EXPRESSION TAG SEQADV 8PET PHE C 2 UNP P14867 EXPRESSION TAG SEQADV 8PET GLU C 3 UNP P14867 EXPRESSION TAG SEQADV 8PET LYS C 4 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C 5 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C 6 UNP P14867 EXPRESSION TAG SEQADV 8PET SER C 7 UNP P14867 EXPRESSION TAG SEQADV 8PET THR C 8 UNP P14867 EXPRESSION TAG SEQADV 8PET GLY C 9 UNP P14867 EXPRESSION TAG SEQADV 8PET MET E -39 UNP P28472 INITIATING METHIONINE SEQADV 8PET GLY E -38 UNP P28472 EXPRESSION TAG SEQADV 8PET ILE E -37 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU E -36 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO E -35 UNP P28472 EXPRESSION TAG SEQADV 8PET SER E -34 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO E -33 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY E -32 UNP P28472 EXPRESSION TAG SEQADV 8PET MET E -31 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO E -30 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA E -29 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU E -28 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU E -27 UNP P28472 EXPRESSION TAG SEQADV 8PET SER E -26 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU E -25 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL E -24 UNP P28472 EXPRESSION TAG SEQADV 8PET SER E -23 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU E -22 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU E -21 UNP P28472 EXPRESSION TAG SEQADV 8PET SER E -20 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL E -19 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU E -18 UNP P28472 EXPRESSION TAG SEQADV 8PET LEU E -17 UNP P28472 EXPRESSION TAG SEQADV 8PET MET E -16 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY E -15 UNP P28472 EXPRESSION TAG SEQADV 8PET CYS E -14 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL E -13 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA E -12 UNP P28472 EXPRESSION TAG SEQADV 8PET GLU E -11 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR E -10 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO E -9 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR E -8 UNP P28472 EXPRESSION TAG SEQADV 8PET ASP E -7 UNP P28472 EXPRESSION TAG SEQADV 8PET VAL E -6 UNP P28472 EXPRESSION TAG SEQADV 8PET PRO E -5 UNP P28472 EXPRESSION TAG SEQADV 8PET ASP E -4 UNP P28472 EXPRESSION TAG SEQADV 8PET TYR E -3 UNP P28472 EXPRESSION TAG SEQADV 8PET ALA E -2 UNP P28472 EXPRESSION TAG SEQADV 8PET THR E -1 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY E 0 UNP P28472 EXPRESSION TAG SEQADV 8PET GLY E 424 UNP P28472 INSERTION SEQADV 8PET THR E 425 UNP P28472 INSERTION SEQRES 1 A 484 MET GLY ILE LEU PRO SER PRO GLY MET PRO ALA LEU LEU SEQRES 2 A 484 SER LEU VAL SER LEU LEU SER VAL LEU LEU MET GLY CYS SEQRES 3 A 484 VAL ALA GLU THR GLY TRP SER HIS PRO GLN PHE GLU LYS SEQRES 4 A 484 GLY GLY GLY SER GLY GLY GLY SER GLY GLY SER ALA TRP SEQRES 5 A 484 SER HIS PRO GLN PHE GLU LYS GLY GLY SER THR GLY ASP SEQRES 6 A 484 ASN THR THR VAL PHE THR ARG ILE LEU ASP ARG LEU LEU SEQRES 7 A 484 ASP GLY TYR ASP ASN ARG LEU ARG PRO GLY LEU GLY GLU SEQRES 8 A 484 ARG VAL THR GLU VAL LYS THR ASP ILE PHE VAL THR SER SEQRES 9 A 484 PHE GLY PRO VAL SER ASP HIS ASP MET GLU TYR THR ILE SEQRES 10 A 484 ASP VAL PHE PHE ARG GLN SER TRP LYS ASP GLU ARG LEU SEQRES 11 A 484 LYS PHE LYS GLY PRO MET THR VAL LEU ARG LEU ASN ASN SEQRES 12 A 484 LEU MET ALA SER LYS ILE TRP THR PRO ASP THR PHE PHE SEQRES 13 A 484 HIS ASN GLY LYS LYS SER VAL ALA HIS ASN MET THR MET SEQRES 14 A 484 PRO ASN LYS LEU LEU ARG ILE THR GLU ASP GLY THR LEU SEQRES 15 A 484 LEU TYR THR MET ARG LEU THR VAL ARG ALA GLU CYS PRO SEQRES 16 A 484 MET HIS LEU GLU ASP PHE PRO MET ASP ALA HIS ALA CYS SEQRES 17 A 484 PRO LEU LYS PHE GLY SER TYR ALA TYR THR ARG ALA GLU SEQRES 18 A 484 VAL VAL TYR GLU TRP THR ARG GLU PRO ALA ARG SER VAL SEQRES 19 A 484 VAL VAL ALA GLU ASP GLY SER ARG LEU ASN GLN TYR ASP SEQRES 20 A 484 LEU LEU GLY GLN THR VAL ASP SER GLY ILE VAL GLN SER SEQRES 21 A 484 SER THR GLY GLU TYR VAL VAL MET THR THR HIS PHE HIS SEQRES 22 A 484 LEU LYS ARG LYS ILE GLY TYR PHE VAL ILE GLN THR TYR SEQRES 23 A 484 LEU PRO CYS ILE MET THR VAL ILE LEU SER GLN VAL SER SEQRES 24 A 484 PHE TRP LEU ASN ARG GLU SER VAL PRO ALA ARG THR VAL SEQRES 25 A 484 PHE GLY VAL THR THR VAL LEU THR MET THR THR LEU SER SEQRES 26 A 484 ILE SER ALA ARG ASN SER LEU PRO LYS VAL ALA TYR ALA SEQRES 27 A 484 THR ALA MET ASP TRP PHE ILE ALA VAL CYS TYR ALA PHE SEQRES 28 A 484 VAL PHE SER ALA LEU ILE GLU PHE ALA THR VAL ASN TYR SEQRES 29 A 484 PHE THR LYS ARG GLY TYR ALA TRP ASP GLY LYS SER VAL SEQRES 30 A 484 VAL PRO GLU LYS PRO LYS LYS VAL LYS ASP PRO LEU ILE SEQRES 31 A 484 LYS LYS ASN ASN THR TYR ALA PRO THR ALA THR SER TYR SEQRES 32 A 484 THR PRO ASN LEU ALA ARG GLY ASP PRO GLY LEU ALA THR SEQRES 33 A 484 ILE ALA LYS SER ALA THR ILE GLU PRO LYS GLU VAL LYS SEQRES 34 A 484 PRO GLU THR LYS PRO PRO GLU PRO LYS LYS THR PHE ASN SEQRES 35 A 484 SER VAL SER LYS ILE ASP ARG LEU SER ARG ILE ALA PHE SEQRES 36 A 484 PRO LEU LEU PHE GLY ILE PHE ASN LEU VAL TYR TRP ALA SEQRES 37 A 484 THR TYR LEU ASN ARG GLU PRO GLN LEU LYS ALA PRO THR SEQRES 38 A 484 PRO HIS GLN SEQRES 1 B 490 MET GLY ILE LEU PRO SER PRO GLY MET PRO ALA LEU LEU SEQRES 2 B 490 SER LEU VAL SER LEU LEU SER VAL LEU LEU MET GLY CYS SEQRES 3 B 490 VAL ALA GLU TYR PRO TYR ASP VAL PRO ASP TYR ALA THR SEQRES 4 B 490 GLY GLN SER VAL ASN ASP PRO GLY ASN MET SER PHE VAL SEQRES 5 B 490 LYS GLU THR VAL ASP LYS LEU LEU LYS GLY TYR ASP ILE SEQRES 6 B 490 ARG LEU ARG PRO ASP PHE GLY GLY PRO PRO VAL CYS VAL SEQRES 7 B 490 GLY MET ASN ILE ASP ILE ALA SER ILE ASP MET VAL SER SEQRES 8 B 490 GLU VAL ASN MET ASP TYR THR LEU THR MET TYR PHE GLN SEQRES 9 B 490 GLN TYR TRP ARG ASP LYS ARG LEU ALA TYR SER GLY ILE SEQRES 10 B 490 PRO LEU ASN LEU THR LEU ASP ASN ARG VAL ALA ASP GLN SEQRES 11 B 490 LEU TRP VAL PRO ASP THR TYR PHE LEU ASN ASP LYS LYS SEQRES 12 B 490 SER PHE VAL HIS GLY VAL THR VAL LYS ASN ARG MET ILE SEQRES 13 B 490 ARG LEU HIS PRO ASP GLY THR VAL LEU TYR GLY LEU ARG SEQRES 14 B 490 ILE THR THR THR ALA ALA CYS MET MET ASP LEU ARG ARG SEQRES 15 B 490 TYR PRO LEU ASP GLU GLN ASN CYS THR LEU GLU ILE GLU SEQRES 16 B 490 SER TYR GLY TYR THR THR ASP ASP ILE GLU PHE TYR TRP SEQRES 17 B 490 ARG GLY GLY ASP LYS ALA VAL THR GLY VAL GLU ARG ILE SEQRES 18 B 490 GLU LEU PRO GLN PHE SER ILE VAL GLU HIS ARG LEU VAL SEQRES 19 B 490 SER ARG ASN VAL VAL PHE ALA THR GLY ALA TYR PRO ARG SEQRES 20 B 490 LEU SER LEU SER PHE ARG LEU LYS ARG ASN ILE GLY TYR SEQRES 21 B 490 PHE ILE LEU GLN THR TYR MET PRO SER ILE LEU ILE THR SEQRES 22 B 490 ILE LEU SER TRP VAL SER PHE TRP ILE ASN TYR ASP ALA SEQRES 23 B 490 SER ALA ALA ARG VAL ALA LEU GLY ILE THR THR VAL LEU SEQRES 24 B 490 THR MET THR THR ILE ASN THR HIS LEU ARG GLU THR LEU SEQRES 25 B 490 PRO LYS ILE PRO TYR VAL LYS ALA ILE ASP MET TYR LEU SEQRES 26 B 490 MET GLY CYS PHE VAL PHE VAL PHE LEU ALA LEU LEU GLU SEQRES 27 B 490 TYR ALA PHE VAL ASN TYR ILE PHE PHE GLY ARG GLY PRO SEQRES 28 B 490 GLN ARG GLN LYS LYS LEU ALA GLU LYS THR ALA LYS ALA SEQRES 29 B 490 LYS ASN ASP ARG SER LYS SER GLU SER ASN ARG VAL ASP SEQRES 30 B 490 ALA HIS GLY ASN ILE LEU LEU THR SER LEU GLU VAL HIS SEQRES 31 B 490 ASN GLU MET ASN GLU VAL SER GLY GLY ILE GLY ASP THR SEQRES 32 B 490 ARG ASN SER ALA ILE SER PHE ASP ASN SER GLY ILE GLN SEQRES 33 B 490 TYR ARG LYS GLN SER MET PRO ARG GLU GLY HIS GLY ARG SEQRES 34 B 490 PHE LEU GLY ASP ARG SER LEU PRO HIS LYS LYS GLY THR SEQRES 35 B 490 THR HIS LEU ARG ARG ARG SER SER GLN LEU LYS ILE LYS SEQRES 36 B 490 ILE PRO ASP LEU THR ASP VAL ASN ALA ILE ASP ARG TRP SEQRES 37 B 490 SER ARG ILE VAL PHE PRO PHE THR PHE SER LEU PHE ASN SEQRES 38 B 490 LEU VAL TYR TRP LEU TYR TYR VAL ASN SEQRES 1 D 490 MET GLY ILE LEU PRO SER PRO GLY MET PRO ALA LEU LEU SEQRES 2 D 490 SER LEU VAL SER LEU LEU SER VAL LEU LEU MET GLY CYS SEQRES 3 D 490 VAL ALA GLU TYR PRO TYR ASP VAL PRO ASP TYR ALA THR SEQRES 4 D 490 GLY GLN SER VAL ASN ASP PRO GLY ASN MET SER PHE VAL SEQRES 5 D 490 LYS GLU THR VAL ASP LYS LEU LEU LYS GLY TYR ASP ILE SEQRES 6 D 490 ARG LEU ARG PRO ASP PHE GLY GLY PRO PRO VAL CYS VAL SEQRES 7 D 490 GLY MET ASN ILE ASP ILE ALA SER ILE ASP MET VAL SER SEQRES 8 D 490 GLU VAL ASN MET ASP TYR THR LEU THR MET TYR PHE GLN SEQRES 9 D 490 GLN TYR TRP ARG ASP LYS ARG LEU ALA TYR SER GLY ILE SEQRES 10 D 490 PRO LEU ASN LEU THR LEU ASP ASN ARG VAL ALA ASP GLN SEQRES 11 D 490 LEU TRP VAL PRO ASP THR TYR PHE LEU ASN ASP LYS LYS SEQRES 12 D 490 SER PHE VAL HIS GLY VAL THR VAL LYS ASN ARG MET ILE SEQRES 13 D 490 ARG LEU HIS PRO ASP GLY THR VAL LEU TYR GLY LEU ARG SEQRES 14 D 490 ILE THR THR THR ALA ALA CYS MET MET ASP LEU ARG ARG SEQRES 15 D 490 TYR PRO LEU ASP GLU GLN ASN CYS THR LEU GLU ILE GLU SEQRES 16 D 490 SER TYR GLY TYR THR THR ASP ASP ILE GLU PHE TYR TRP SEQRES 17 D 490 ARG GLY GLY ASP LYS ALA VAL THR GLY VAL GLU ARG ILE SEQRES 18 D 490 GLU LEU PRO GLN PHE SER ILE VAL GLU HIS ARG LEU VAL SEQRES 19 D 490 SER ARG ASN VAL VAL PHE ALA THR GLY ALA TYR PRO ARG SEQRES 20 D 490 LEU SER LEU SER PHE ARG LEU LYS ARG ASN ILE GLY TYR SEQRES 21 D 490 PHE ILE LEU GLN THR TYR MET PRO SER ILE LEU ILE THR SEQRES 22 D 490 ILE LEU SER TRP VAL SER PHE TRP ILE ASN TYR ASP ALA SEQRES 23 D 490 SER ALA ALA ARG VAL ALA LEU GLY ILE THR THR VAL LEU SEQRES 24 D 490 THR MET THR THR ILE ASN THR HIS LEU ARG GLU THR LEU SEQRES 25 D 490 PRO LYS ILE PRO TYR VAL LYS ALA ILE ASP MET TYR LEU SEQRES 26 D 490 MET GLY CYS PHE VAL PHE VAL PHE LEU ALA LEU LEU GLU SEQRES 27 D 490 TYR ALA PHE VAL ASN TYR ILE PHE PHE GLY ARG GLY PRO SEQRES 28 D 490 GLN ARG GLN LYS LYS LEU ALA GLU LYS THR ALA LYS ALA SEQRES 29 D 490 LYS ASN ASP ARG SER LYS SER GLU SER ASN ARG VAL ASP SEQRES 30 D 490 ALA HIS GLY ASN ILE LEU LEU THR SER LEU GLU VAL HIS SEQRES 31 D 490 ASN GLU MET ASN GLU VAL SER GLY GLY ILE GLY ASP THR SEQRES 32 D 490 ARG ASN SER ALA ILE SER PHE ASP ASN SER GLY ILE GLN SEQRES 33 D 490 TYR ARG LYS GLN SER MET PRO ARG GLU GLY HIS GLY ARG SEQRES 34 D 490 PHE LEU GLY ASP ARG SER LEU PRO HIS LYS LYS GLY THR SEQRES 35 D 490 THR HIS LEU ARG ARG ARG SER SER GLN LEU LYS ILE LYS SEQRES 36 D 490 ILE PRO ASP LEU THR ASP VAL ASN ALA ILE ASP ARG TRP SEQRES 37 D 490 SER ARG ILE VAL PHE PRO PHE THR PHE SER LEU PHE ASN SEQRES 38 D 490 LEU VAL TYR TRP LEU TYR TYR VAL ASN SEQRES 1 C 484 MET GLY ILE LEU PRO SER PRO GLY MET PRO ALA LEU LEU SEQRES 2 C 484 SER LEU VAL SER LEU LEU SER VAL LEU LEU MET GLY CYS SEQRES 3 C 484 VAL ALA GLU THR GLY TRP SER HIS PRO GLN PHE GLU LYS SEQRES 4 C 484 GLY GLY GLY SER GLY GLY GLY SER GLY GLY SER ALA TRP SEQRES 5 C 484 SER HIS PRO GLN PHE GLU LYS GLY GLY SER THR GLY ASP SEQRES 6 C 484 ASN THR THR VAL PHE THR ARG ILE LEU ASP ARG LEU LEU SEQRES 7 C 484 ASP GLY TYR ASP ASN ARG LEU ARG PRO GLY LEU GLY GLU SEQRES 8 C 484 ARG VAL THR GLU VAL LYS THR ASP ILE PHE VAL THR SER SEQRES 9 C 484 PHE GLY PRO VAL SER ASP HIS ASP MET GLU TYR THR ILE SEQRES 10 C 484 ASP VAL PHE PHE ARG GLN SER TRP LYS ASP GLU ARG LEU SEQRES 11 C 484 LYS PHE LYS GLY PRO MET THR VAL LEU ARG LEU ASN ASN SEQRES 12 C 484 LEU MET ALA SER LYS ILE TRP THR PRO ASP THR PHE PHE SEQRES 13 C 484 HIS ASN GLY LYS LYS SER VAL ALA HIS ASN MET THR MET SEQRES 14 C 484 PRO ASN LYS LEU LEU ARG ILE THR GLU ASP GLY THR LEU SEQRES 15 C 484 LEU TYR THR MET ARG LEU THR VAL ARG ALA GLU CYS PRO SEQRES 16 C 484 MET HIS LEU GLU ASP PHE PRO MET ASP ALA HIS ALA CYS SEQRES 17 C 484 PRO LEU LYS PHE GLY SER TYR ALA TYR THR ARG ALA GLU SEQRES 18 C 484 VAL VAL TYR GLU TRP THR ARG GLU PRO ALA ARG SER VAL SEQRES 19 C 484 VAL VAL ALA GLU ASP GLY SER ARG LEU ASN GLN TYR ASP SEQRES 20 C 484 LEU LEU GLY GLN THR VAL ASP SER GLY ILE VAL GLN SER SEQRES 21 C 484 SER THR GLY GLU TYR VAL VAL MET THR THR HIS PHE HIS SEQRES 22 C 484 LEU LYS ARG LYS ILE GLY TYR PHE VAL ILE GLN THR TYR SEQRES 23 C 484 LEU PRO CYS ILE MET THR VAL ILE LEU SER GLN VAL SER SEQRES 24 C 484 PHE TRP LEU ASN ARG GLU SER VAL PRO ALA ARG THR VAL SEQRES 25 C 484 PHE GLY VAL THR THR VAL LEU THR MET THR THR LEU SER SEQRES 26 C 484 ILE SER ALA ARG ASN SER LEU PRO LYS VAL ALA TYR ALA SEQRES 27 C 484 THR ALA MET ASP TRP PHE ILE ALA VAL CYS TYR ALA PHE SEQRES 28 C 484 VAL PHE SER ALA LEU ILE GLU PHE ALA THR VAL ASN TYR SEQRES 29 C 484 PHE THR LYS ARG GLY TYR ALA TRP ASP GLY LYS SER VAL SEQRES 30 C 484 VAL PRO GLU LYS PRO LYS LYS VAL LYS ASP PRO LEU ILE SEQRES 31 C 484 LYS LYS ASN ASN THR TYR ALA PRO THR ALA THR SER TYR SEQRES 32 C 484 THR PRO ASN LEU ALA ARG GLY ASP PRO GLY LEU ALA THR SEQRES 33 C 484 ILE ALA LYS SER ALA THR ILE GLU PRO LYS GLU VAL LYS SEQRES 34 C 484 PRO GLU THR LYS PRO PRO GLU PRO LYS LYS THR PHE ASN SEQRES 35 C 484 SER VAL SER LYS ILE ASP ARG LEU SER ARG ILE ALA PHE SEQRES 36 C 484 PRO LEU LEU PHE GLY ILE PHE ASN LEU VAL TYR TRP ALA SEQRES 37 C 484 THR TYR LEU ASN ARG GLU PRO GLN LEU LYS ALA PRO THR SEQRES 38 C 484 PRO HIS GLN SEQRES 1 E 490 MET GLY ILE LEU PRO SER PRO GLY MET PRO ALA LEU LEU SEQRES 2 E 490 SER LEU VAL SER LEU LEU SER VAL LEU LEU MET GLY CYS SEQRES 3 E 490 VAL ALA GLU TYR PRO TYR ASP VAL PRO ASP TYR ALA THR SEQRES 4 E 490 GLY GLN SER VAL ASN ASP PRO GLY ASN MET SER PHE VAL SEQRES 5 E 490 LYS GLU THR VAL ASP LYS LEU LEU LYS GLY TYR ASP ILE SEQRES 6 E 490 ARG LEU ARG PRO ASP PHE GLY GLY PRO PRO VAL CYS VAL SEQRES 7 E 490 GLY MET ASN ILE ASP ILE ALA SER ILE ASP MET VAL SER SEQRES 8 E 490 GLU VAL ASN MET ASP TYR THR LEU THR MET TYR PHE GLN SEQRES 9 E 490 GLN TYR TRP ARG ASP LYS ARG LEU ALA TYR SER GLY ILE SEQRES 10 E 490 PRO LEU ASN LEU THR LEU ASP ASN ARG VAL ALA ASP GLN SEQRES 11 E 490 LEU TRP VAL PRO ASP THR TYR PHE LEU ASN ASP LYS LYS SEQRES 12 E 490 SER PHE VAL HIS GLY VAL THR VAL LYS ASN ARG MET ILE SEQRES 13 E 490 ARG LEU HIS PRO ASP GLY THR VAL LEU TYR GLY LEU ARG SEQRES 14 E 490 ILE THR THR THR ALA ALA CYS MET MET ASP LEU ARG ARG SEQRES 15 E 490 TYR PRO LEU ASP GLU GLN ASN CYS THR LEU GLU ILE GLU SEQRES 16 E 490 SER TYR GLY TYR THR THR ASP ASP ILE GLU PHE TYR TRP SEQRES 17 E 490 ARG GLY GLY ASP LYS ALA VAL THR GLY VAL GLU ARG ILE SEQRES 18 E 490 GLU LEU PRO GLN PHE SER ILE VAL GLU HIS ARG LEU VAL SEQRES 19 E 490 SER ARG ASN VAL VAL PHE ALA THR GLY ALA TYR PRO ARG SEQRES 20 E 490 LEU SER LEU SER PHE ARG LEU LYS ARG ASN ILE GLY TYR SEQRES 21 E 490 PHE ILE LEU GLN THR TYR MET PRO SER ILE LEU ILE THR SEQRES 22 E 490 ILE LEU SER TRP VAL SER PHE TRP ILE ASN TYR ASP ALA SEQRES 23 E 490 SER ALA ALA ARG VAL ALA LEU GLY ILE THR THR VAL LEU SEQRES 24 E 490 THR MET THR THR ILE ASN THR HIS LEU ARG GLU THR LEU SEQRES 25 E 490 PRO LYS ILE PRO TYR VAL LYS ALA ILE ASP MET TYR LEU SEQRES 26 E 490 MET GLY CYS PHE VAL PHE VAL PHE LEU ALA LEU LEU GLU SEQRES 27 E 490 TYR ALA PHE VAL ASN TYR ILE PHE PHE GLY ARG GLY PRO SEQRES 28 E 490 GLN ARG GLN LYS LYS LEU ALA GLU LYS THR ALA LYS ALA SEQRES 29 E 490 LYS ASN ASP ARG SER LYS SER GLU SER ASN ARG VAL ASP SEQRES 30 E 490 ALA HIS GLY ASN ILE LEU LEU THR SER LEU GLU VAL HIS SEQRES 31 E 490 ASN GLU MET ASN GLU VAL SER GLY GLY ILE GLY ASP THR SEQRES 32 E 490 ARG ASN SER ALA ILE SER PHE ASP ASN SER GLY ILE GLN SEQRES 33 E 490 TYR ARG LYS GLN SER MET PRO ARG GLU GLY HIS GLY ARG SEQRES 34 E 490 PHE LEU GLY ASP ARG SER LEU PRO HIS LYS LYS GLY THR SEQRES 35 E 490 THR HIS LEU ARG ARG ARG SER SER GLN LEU LYS ILE LYS SEQRES 36 E 490 ILE PRO ASP LEU THR ASP VAL ASN ALA ILE ASP ARG TRP SEQRES 37 E 490 SER ARG ILE VAL PHE PRO PHE THR PHE SER LEU PHE ASN SEQRES 38 E 490 LEU VAL TYR TRP LEU TYR TYR VAL ASN SEQRES 1 F 135 GLN VAL GLN LEU GLN GLU SER GLY GLY GLY LEU VAL GLN SEQRES 2 F 135 ALA GLY GLY SER LEU ARG LEU SER CYS ALA ALA SER GLY SEQRES 3 F 135 HIS THR PHE ASN TYR PRO ILE MET GLY TRP PHE ARG GLN SEQRES 4 F 135 ALA PRO GLY LYS GLU ARG GLU PHE VAL GLY ALA ILE SER SEQRES 5 F 135 TRP SER GLY GLY SER THR SER TYR ALA ASP SER VAL LYS SEQRES 6 F 135 ASP ARG PHE THR ILE SER ARG ASP ASN ALA LYS ASN THR SEQRES 7 F 135 VAL TYR LEU GLU MET ASN ASN LEU LYS PRO GLU ASP THR SEQRES 8 F 135 ALA VAL TYR TYR CYS ALA ALA LYS GLY ARG TYR SER GLY SEQRES 9 F 135 GLY LEU TYR TYR PRO THR ASN TYR ASP TYR TRP GLY GLN SEQRES 10 F 135 GLY THR GLN VAL THR VAL SER SER HIS HIS HIS HIS HIS SEQRES 11 F 135 HIS GLU PRO GLU ALA HET NAG a 1 26 HET NAG a 2 26 HET BMA a 3 19 HET MAN a 4 21 HET MAN a 5 21 HET NAG b 1 26 HET NAG b 2 26 HET BMA b 3 19 HET MAN b 4 21 HET MAN b 5 21 HET NAG G 1 26 HET NAG G 2 27 HET NAG c 1 26 HET NAG c 2 26 HET BMA c 3 19 HET MAN c 4 21 HET MAN c 5 21 HET NAG H 1 26 HET NAG H 2 27 HET NAG d 1 26 HET NAG d 2 26 HET BMA d 3 19 HET MAN d 4 21 HET MAN d 5 21 HET NAG e 1 26 HET NAG e 2 26 HET BMA e 3 19 HET MAN e 4 21 HET MAN e 5 21 HET NAG I 1 26 HET NAG I 2 27 HET R16 A 501 50 HET PIO A 502 91 HET D10 B 501 32 HET D10 B 502 32 HET D10 B 503 32 HET D10 B 504 32 HET ZN B 505 1 HET D10 D 501 32 HET D10 D 502 32 HET D10 D 503 32 HET D10 D 504 32 HET D10 D 505 32 HET R16 C 501 50 HET PIO C 502 91 HET D10 E 501 32 HET D10 E 502 32 HET D10 E 503 32 HETNAM NAG 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSE HETNAM BMA BETA-D-MANNOPYRANOSE HETNAM MAN ALPHA-D-MANNOPYRANOSE HETNAM R16 HEXADECANE HETNAM PIO [(2R)-2-OCTANOYLOXY-3-[OXIDANYL-[(1R,2R,3S,4R,5R,6S)-2, HETNAM 2 PIO 3,6-TRIS(OXIDANYL)-4,5-DIPHOSPHONOOXY-CYCLOHEXYL]OXY- HETNAM 3 PIO PHOSPHORYL]OXY-PROPYL] OCTANOATE HETNAM D10 DECANE HETNAM ZN ZINC ION HETSYN NAG N-ACETYL-BETA-D-GLUCOSAMINE; 2-ACETAMIDO-2-DEOXY-BETA- HETSYN 2 NAG D-GLUCOSE; 2-ACETAMIDO-2-DEOXY-D-GLUCOSE; 2-ACETAMIDO- HETSYN 3 NAG 2-DEOXY-GLUCOSE; N-ACETYL-D-GLUCOSAMINE HETSYN BMA BETA-D-MANNOSE; D-MANNOSE; MANNOSE HETSYN MAN ALPHA-D-MANNOSE; D-MANNOSE; MANNOSE HETSYN PIO DIOCTANOYL L-ALPHA-PHOSPHATIDYL-D-MYO-INOSITOL 4,5- HETSYN 2 PIO DIPHOSPHATE FORMUL 7 NAG 16(C8 H15 N O6) FORMUL 7 BMA 5(C6 H12 O6) FORMUL 7 MAN 10(C6 H12 O6) FORMUL 15 R16 2(C16 H34) FORMUL 16 PIO 2(C25 H49 O19 P3) FORMUL 17 D10 12(C10 H22) FORMUL 21 ZN ZN 2+ FORMUL 32 HOH *84(H2 O) HELIX 1 AA1 THR A 12 LEU A 23 1 12 HELIX 2 AA2 GLU A 73 LYS A 76 5 4 HELIX 3 AA3 ASN A 87 SER A 92 1 6 HELIX 4 AA4 GLU A 174 ARG A 177 5 4 HELIX 5 AA5 ILE A 223 THR A 230 1 8 HELIX 6 AA6 THR A 230 VAL A 243 1 14 HELIX 7 AA7 SER A 244 LEU A 247 5 4 HELIX 8 AA8 SER A 251 LEU A 277 1 27 HELIX 9 AA9 THR A 284 TYR A 309 1 26 HELIX 10 AB1 SER A 390 LEU A 416 1 27 HELIX 11 AB2 SER B 10 LYS B 21 1 12 HELIX 12 AB3 LYS B 70 ALA B 73 5 4 HELIX 13 AB4 ASP B 84 LEU B 91 5 8 HELIX 14 AB5 GLY B 170 LYS B 173 5 4 HELIX 15 AB6 GLY B 177 ILE B 181 5 5 HELIX 16 AB7 ILE B 218 THR B 225 1 8 HELIX 17 AB8 THR B 225 TRP B 237 1 13 HELIX 18 AB9 VAL B 238 ILE B 242 5 5 HELIX 19 AC1 ALA B 246 THR B 271 1 26 HELIX 20 AC2 LYS B 279 GLY B 308 1 30 HELIX 21 AC3 VAL B 445 VAL B 472 1 28 HELIX 22 AC4 SER D 10 LYS D 21 1 12 HELIX 23 AC5 LYS D 70 ALA D 73 5 4 HELIX 24 AC6 ASP D 84 LEU D 91 5 8 HELIX 25 AC7 GLY D 170 LYS D 173 5 4 HELIX 26 AC8 GLY D 177 ILE D 181 5 5 HELIX 27 AC9 ILE D 218 THR D 225 1 8 HELIX 28 AD1 THR D 225 TRP D 237 1 13 HELIX 29 AD2 VAL D 238 ILE D 242 5 5 HELIX 30 AD3 ALA D 246 LEU D 272 1 27 HELIX 31 AD4 LYS D 279 GLY D 308 1 30 HELIX 32 AD5 VAL D 445 VAL D 472 1 28 HELIX 33 AD6 THR C 13 LEU C 23 1 11 HELIX 34 AD7 GLU C 73 LYS C 76 5 4 HELIX 35 AD8 ASN C 87 SER C 92 1 6 HELIX 36 AD9 HIS C 142 PHE C 146 5 5 HELIX 37 AE1 GLU C 174 SER C 178 1 5 HELIX 38 AE2 ILE C 223 THR C 230 1 8 HELIX 39 AE3 THR C 230 SER C 244 1 15 HELIX 40 AE4 PHE C 245 LEU C 247 5 3 HELIX 41 AE5 SER C 251 LEU C 277 1 27 HELIX 42 AE6 THR C 284 PHE C 310 1 27 HELIX 43 AE7 SER C 390 LEU C 416 1 27 HELIX 44 AE8 SER E 10 LYS E 21 1 12 HELIX 45 AE9 LYS E 70 ALA E 73 5 4 HELIX 46 AF1 ASP E 84 LEU E 91 5 8 HELIX 47 AF2 GLY E 170 LYS E 173 5 4 HELIX 48 AF3 ILE E 218 THR E 225 1 8 HELIX 49 AF4 THR E 225 TRP E 237 1 13 HELIX 50 AF5 VAL E 238 ILE E 242 5 5 HELIX 51 AF6 ALA E 246 HIS E 267 1 22 HELIX 52 AF7 LEU E 268 LEU E 272 5 5 HELIX 53 AF8 LYS E 279 GLY E 308 1 30 HELIX 54 AF9 VAL E 445 VAL E 472 1 28 HELIX 55 AG1 LYS F 87 THR F 91 5 5 HELIX 56 AG2 TYR F 108 TYR F 112 5 5 SHEET 1 AA1 6 VAL A 83 ARG A 85 0 SHEET 2 AA1 6 LYS A 117 THR A 122 -1 O ILE A 121 N LEU A 84 SHEET 3 AA1 6 THR A 126 GLU A 138 -1 O LEU A 128 N ARG A 120 SHEET 4 AA1 6 GLU A 59 LYS A 71 -1 N GLN A 68 O TYR A 129 SHEET 5 AA1 6 THR A 39 SER A 54 -1 N SER A 54 O GLU A 59 SHEET 6 AA1 6 VAL A 167 TRP A 171 1 O GLU A 170 N THR A 43 SHEET 1 AA2 5 VAL A 108 ALA A 109 0 SHEET 2 AA2 5 THR A 126 GLU A 138 -1 O THR A 134 N VAL A 108 SHEET 3 AA2 5 GLU A 59 LYS A 71 -1 N GLN A 68 O TYR A 129 SHEET 4 AA2 5 THR A 39 SER A 54 -1 N SER A 54 O GLU A 59 SHEET 5 AA2 5 VAL A 179 VAL A 181 1 O VAL A 180 N VAL A 47 SHEET 1 AA3 4 THR A 99 PHE A 101 0 SHEET 2 AA3 4 ALA A 150 SER A 159 -1 O GLY A 158 N PHE A 100 SHEET 3 AA3 4 GLY A 208 ARG A 221 -1 O LEU A 219 N HIS A 151 SHEET 4 AA3 4 TYR A 191 SER A 205 -1 N VAL A 203 O TYR A 210 SHEET 1 AA4 6 LEU B 81 LEU B 83 0 SHEET 2 AA4 6 ARG B 114 LEU B 118 -1 O LEU B 118 N LEU B 81 SHEET 3 AA4 6 THR B 123 ALA B 135 -1 O LEU B 125 N ARG B 117 SHEET 4 AA4 6 ASP B 56 ARG B 68 -1 N TRP B 67 O VAL B 124 SHEET 5 AA4 6 VAL B 36 SER B 51 -1 N GLY B 39 O TYR B 66 SHEET 6 AA4 6 ILE B 164 TRP B 168 1 O GLU B 165 N VAL B 38 SHEET 1 AA5 5 ASP B 101 VAL B 106 0 SHEET 2 AA5 5 THR B 123 ALA B 135 -1 O THR B 133 N LYS B 102 SHEET 3 AA5 5 ASP B 56 ARG B 68 -1 N TRP B 67 O VAL B 124 SHEET 4 AA5 5 VAL B 36 SER B 51 -1 N GLY B 39 O TYR B 66 SHEET 5 AA5 5 VAL B 175 THR B 176 1 O THR B 176 N ILE B 44 SHEET 1 AA6 4 THR B 96 PHE B 98 0 SHEET 2 AA6 4 GLU B 147 SER B 156 -1 O GLU B 155 N TYR B 97 SHEET 3 AA6 4 ALA B 204 LYS B 215 -1 O LEU B 208 N ILE B 154 SHEET 4 AA6 4 ILE B 188 VAL B 199 -1 N VAL B 198 O TYR B 205 SHEET 1 AA7 6 THR D 82 LEU D 83 0 SHEET 2 AA7 6 ARG D 114 LEU D 118 -1 O ILE D 116 N LEU D 83 SHEET 3 AA7 6 THR D 123 ALA D 135 -1 O LEU D 125 N ARG D 117 SHEET 4 AA7 6 ASP D 56 ARG D 68 -1 N PHE D 63 O LEU D 128 SHEET 5 AA7 6 VAL D 36 SER D 51 -1 N GLY D 39 O TYR D 66 SHEET 6 AA7 6 ILE D 164 TRP D 168 1 O GLU D 165 N VAL D 38 SHEET 1 AA8 5 ASP D 101 VAL D 106 0 SHEET 2 AA8 5 THR D 123 ALA D 135 -1 O THR D 133 N LYS D 103 SHEET 3 AA8 5 ASP D 56 ARG D 68 -1 N PHE D 63 O LEU D 128 SHEET 4 AA8 5 VAL D 36 SER D 51 -1 N GLY D 39 O TYR D 66 SHEET 5 AA8 5 VAL D 175 THR D 176 1 O THR D 176 N ILE D 44 SHEET 1 AA9 4 THR D 96 PHE D 98 0 SHEET 2 AA9 4 GLU D 147 SER D 156 -1 O GLU D 155 N TYR D 97 SHEET 3 AA9 4 GLY D 203 ARG D 216 -1 O LEU D 214 N GLN D 148 SHEET 4 AA9 4 PHE D 186 PHE D 200 -1 N PHE D 200 O GLY D 203 SHEET 1 AB1 6 VAL C 83 LEU C 86 0 SHEET 2 AB1 6 LYS C 117 THR C 122 -1 O ILE C 121 N LEU C 84 SHEET 3 AB1 6 THR C 126 GLU C 138 -1 O LEU C 128 N ARG C 120 SHEET 4 AB1 6 GLU C 59 LYS C 71 -1 N TRP C 70 O LEU C 127 SHEET 5 AB1 6 THR C 39 SER C 54 -1 N LYS C 42 O SER C 69 SHEET 6 AB1 6 VAL C 167 TRP C 171 1 O VAL C 168 N THR C 39 SHEET 1 AB2 5 GLY C 104 ALA C 109 0 SHEET 2 AB2 5 THR C 126 GLU C 138 -1 O ARG C 136 N LYS C 105 SHEET 3 AB2 5 GLU C 59 LYS C 71 -1 N TRP C 70 O LEU C 127 SHEET 4 AB2 5 THR C 39 SER C 54 -1 N LYS C 42 O SER C 69 SHEET 5 AB2 5 VAL C 179 VAL C 181 1 O VAL C 180 N VAL C 47 SHEET 1 AB3 4 THR C 99 PHE C 101 0 SHEET 2 AB3 4 ALA C 150 SER C 159 -1 O GLY C 158 N PHE C 100 SHEET 3 AB3 4 GLU C 209 ARG C 221 -1 O LEU C 219 N HIS C 151 SHEET 4 AB3 4 TYR C 191 GLN C 204 -1 N GLY C 201 O VAL C 212 SHEET 1 AB4 6 THR E 82 LEU E 83 0 SHEET 2 AB4 6 ARG E 114 LEU E 118 -1 O ILE E 116 N LEU E 83 SHEET 3 AB4 6 THR E 123 ALA E 135 -1 O LEU E 125 N ARG E 117 SHEET 4 AB4 6 ASP E 56 ARG E 68 -1 N TRP E 67 O VAL E 124 SHEET 5 AB4 6 VAL E 36 SER E 51 -1 N ASP E 43 O TYR E 62 SHEET 6 AB4 6 ILE E 164 TRP E 168 1 O GLU E 165 N VAL E 38 SHEET 1 AB5 5 ASP E 101 VAL E 106 0 SHEET 2 AB5 5 THR E 123 ALA E 135 -1 O THR E 133 N LYS E 102 SHEET 3 AB5 5 ASP E 56 ARG E 68 -1 N TRP E 67 O VAL E 124 SHEET 4 AB5 5 VAL E 36 SER E 51 -1 N ASP E 43 O TYR E 62 SHEET 5 AB5 5 VAL E 175 THR E 176 1 O THR E 176 N ILE E 44 SHEET 1 AB6 4 THR E 96 PHE E 98 0 SHEET 2 AB6 4 GLU E 147 SER E 156 -1 O GLU E 155 N TYR E 97 SHEET 3 AB6 4 GLY E 203 ARG E 216 -1 O LEU E 208 N ILE E 154 SHEET 4 AB6 4 PHE E 186 PHE E 200 -1 N VAL E 198 O TYR E 205 SHEET 1 AB7 4 VAL F 2 SER F 7 0 SHEET 2 AB7 4 SER F 17 GLY F 26 -1 O SER F 21 N SER F 7 SHEET 3 AB7 4 THR F 78 ASN F 84 -1 O MET F 83 N LEU F 18 SHEET 4 AB7 4 PHE F 68 ASP F 73 -1 N SER F 71 O TYR F 80 SHEET 1 AB8 6 LEU F 11 GLN F 13 0 SHEET 2 AB8 6 THR F 119 SER F 124 1 O THR F 122 N VAL F 12 SHEET 3 AB8 6 ALA F 92 LYS F 99 -1 N TYR F 94 O THR F 119 SHEET 4 AB8 6 ILE F 33 GLN F 39 -1 N ILE F 33 O LYS F 99 SHEET 5 AB8 6 ARG F 45 ILE F 51 -1 O GLY F 49 N TRP F 36 SHEET 6 AB8 6 THR F 58 TYR F 60 -1 O SER F 59 N ALA F 50 SHEET 1 AB9 4 LEU F 11 GLN F 13 0 SHEET 2 AB9 4 THR F 119 SER F 124 1 O THR F 122 N VAL F 12 SHEET 3 AB9 4 ALA F 92 LYS F 99 -1 N TYR F 94 O THR F 119 SHEET 4 AB9 4 TYR F 114 TRP F 115 -1 O TYR F 114 N ALA F 98 SSBOND 1 CYS A 139 CYS A 153 1555 1555 2.04 SSBOND 2 CYS B 136 CYS B 150 1555 1555 2.04 SSBOND 3 CYS D 136 CYS D 150 1555 1555 2.04 SSBOND 4 CYS C 139 CYS C 153 1555 1555 2.04 SSBOND 5 CYS E 136 CYS E 150 1555 1555 2.03 SSBOND 6 CYS F 22 CYS F 96 1555 1555 2.03 LINK ND2 ASN A 111 C1 NAG a 1 1555 1555 1.44 LINK ND2 ASN B 80 C1 NAG G 1 1555 1555 1.44 LINK ND2 ASN B 149 C1 NAG b 1 1555 1555 1.44 LINK ND2 ASN D 80 C1 NAG H 1 1555 1555 1.44 LINK ND2 ASN D 149 C1 NAG c 1 1555 1555 1.44 LINK ND2 ASN C 111 C1 NAG d 1 1555 1555 1.44 LINK ND2 ASN E 80 C1 NAG I 1 1555 1555 1.44 LINK ND2 ASN E 149 C1 NAG e 1 1555 1555 1.44 LINK O4 NAG a 1 C1 NAG a 2 1555 1555 1.44 LINK O4 NAG a 2 C1 BMA a 3 1555 1555 1.45 LINK O3 BMA a 3 C1 MAN a 4 1555 1555 1.44 LINK O6 BMA a 3 C1 MAN a 5 1555 1555 1.44 LINK O4 NAG b 1 C1 NAG b 2 1555 1555 1.44 LINK O4 NAG b 2 C1 BMA b 3 1555 1555 1.44 LINK O3 BMA b 3 C1 MAN b 4 1555 1555 1.44 LINK O6 BMA b 3 C1 MAN b 5 1555 1555 1.44 LINK O4 NAG G 1 C1 NAG G 2 1555 1555 1.44 LINK O4 NAG c 1 C1 NAG c 2 1555 1555 1.43 LINK O4 NAG c 2 C1 BMA c 3 1555 1555 1.44 LINK O3 BMA c 3 C1 MAN c 4 1555 1555 1.44 LINK O6 BMA c 3 C1 MAN c 5 1555 1555 1.44 LINK O4 NAG H 1 C1 NAG H 2 1555 1555 1.44 LINK O4 NAG d 1 C1 NAG d 2 1555 1555 1.44 LINK O4 NAG d 2 C1 BMA d 3 1555 1555 1.44 LINK O3 BMA d 3 C1 MAN d 4 1555 1555 1.44 LINK O6 BMA d 3 C1 MAN d 5 1555 1555 1.44 LINK O4 NAG e 1 C1 NAG e 2 1555 1555 1.43 LINK O4 NAG e 2 C1 BMA e 3 1555 1555 1.43 LINK O3 BMA e 3 C1 MAN e 4 1555 1555 1.44 LINK O6 BMA e 3 C1 MAN e 5 1555 1555 1.45 LINK O4 NAG I 1 C1 NAG I 2 1555 1555 1.44 LINK NE2 HIS B 267 ZN ZN B 505 1555 1555 2.30 LINK ZN ZN B 505 NE2 HIS D 267 1555 1555 2.30 LINK ZN ZN B 505 ND1 HIS E 267 1555 1555 2.30 CISPEP 1 MET A 112 THR A 113 0 -7.46 CISPEP 2 PHE A 146 PRO A 147 0 3.70 CISPEP 3 VAL B 109 THR B 110 0 -0.73 CISPEP 4 TYR B 143 PRO B 144 0 4.31 CISPEP 5 VAL D 109 THR D 110 0 -1.47 CISPEP 6 TYR D 143 PRO D 144 0 5.68 CISPEP 7 MET C 112 THR C 113 0 -3.86 CISPEP 8 PHE C 146 PRO C 147 0 5.01 CISPEP 9 VAL E 109 THR E 110 0 -0.51 CISPEP 10 TYR E 143 PRO E 144 0 2.77 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000