HEADER MEMBRANE PROTEIN 02-JAN-24 8XOS TITLE CRYO-EM STRUCTURE OF THE TETHERED AGONIST-BOUND HUMAN PAR1-GI COMPLEX COMPND MOL_ID: 1; COMPND 2 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I) SUBUNIT ALPHA-1; COMPND 3 CHAIN: A; COMPND 4 SYNONYM: ADENYLATE CYCLASE-INHIBITING G ALPHA PROTEIN; COMPND 5 ENGINEERED: YES; COMPND 6 MUTATION: YES; COMPND 7 MOL_ID: 2; COMPND 8 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(T) SUBUNIT COMPND 9 BETA-1; COMPND 10 CHAIN: B; COMPND 11 SYNONYM: TRANSDUCIN BETA CHAIN 1; COMPND 12 ENGINEERED: YES; COMPND 13 MOL_ID: 3; COMPND 14 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(O) SUBUNIT COMPND 15 GAMMA-2; COMPND 16 CHAIN: C; COMPND 17 SYNONYM: G GAMMA-I; COMPND 18 ENGINEERED: YES; COMPND 19 MOL_ID: 4; COMPND 20 MOLECULE: SCFV16; COMPND 21 CHAIN: E; COMPND 22 ENGINEERED: YES; COMPND 23 MOL_ID: 5; COMPND 24 MOLECULE: PROTEINASE-ACTIVATED RECEPTOR 1 LGBIT; COMPND 25 CHAIN: R; COMPND 26 SYNONYM: PAR-1,COAGULATION FACTOR II RECEPTOR,THROMBIN RECEPTOR; COMPND 27 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_COMMON: HUMAN; SOURCE 4 ORGANISM_TAXID: 9606; SOURCE 5 GENE: GNAI1; SOURCE 6 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 7108; SOURCE 8 MOL_ID: 2; SOURCE 9 ORGANISM_SCIENTIFIC: RATTUS; SOURCE 10 ORGANISM_TAXID: 10114; SOURCE 11 GENE: GNB1; SOURCE 12 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA; SOURCE 13 EXPRESSION_SYSTEM_TAXID: 7108; SOURCE 14 MOL_ID: 3; SOURCE 15 ORGANISM_SCIENTIFIC: BOS TAURUS; SOURCE 16 ORGANISM_COMMON: DOMESTIC CATTLE; SOURCE 17 ORGANISM_TAXID: 9913; SOURCE 18 GENE: GNG2; SOURCE 19 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA; SOURCE 20 EXPRESSION_SYSTEM_TAXID: 7108; SOURCE 21 MOL_ID: 4; SOURCE 22 ORGANISM_SCIENTIFIC: MUS MUSCULUS; SOURCE 23 ORGANISM_TAXID: 10090; SOURCE 24 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA; SOURCE 25 EXPRESSION_SYSTEM_TAXID: 7108; SOURCE 26 MOL_ID: 5; SOURCE 27 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 28 ORGANISM_COMMON: HUMAN; SOURCE 29 ORGANISM_TAXID: 9606; SOURCE 30 GENE: F2R, CF2R, PAR1, TR; SOURCE 31 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA; SOURCE 32 EXPRESSION_SYSTEM_TAXID: 7108 KEYWDS GPCR, MEMBRANE PROTEIN, PROTEASE-ACTIVATED RECEPTOR EXPDTA ELECTRON MICROSCOPY AUTHOR J.GUO,Y.ZHANG REVDAT 1 18-SEP-24 8XOS 0 JRNL AUTH J.GUO,Y.L.ZHOU,Y.YANG,S.GUO,E.YOU,X.XIE,Y.JIANG,C.MAO, JRNL AUTH 2 H.E.XU,Y.ZHANG JRNL TITL STRUCTURAL BASIS OF TETHERED AGONISM AND G PROTEIN COUPLING JRNL TITL 2 OF PROTEASE-ACTIVATED RECEPTORS. JRNL REF CELL RES. 2024 JRNL REFN ISSN 1001-0602 JRNL PMID 38997424 JRNL DOI 10.1038/S41422-024-00997-2 REMARK 2 REMARK 2 RESOLUTION. 3.20 ANGSTROMS. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 SOFTWARE PACKAGES : NULL REMARK 3 RECONSTRUCTION SCHEMA : NULL REMARK 3 REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT REMARK 3 PDB ENTRY : NULL REMARK 3 REFINEMENT SPACE : NULL REMARK 3 REFINEMENT PROTOCOL : NULL REMARK 3 REFINEMENT TARGET : NULL REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL REMARK 3 REMARK 3 FITTING PROCEDURE : NULL REMARK 3 REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 3.200 REMARK 3 NUMBER OF PARTICLES : 162724 REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING AND AMPLITUDE REMARK 3 CORRECTION REMARK 3 REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL REMARK 3 REMARK 3 OTHER DETAILS: NULL REMARK 4 REMARK 4 8XOS COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 10-JAN-24. REMARK 100 THE DEPOSITION ID IS D_1300043681. REMARK 245 REMARK 245 EXPERIMENTAL DETAILS REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE REMARK 245 SPECIMEN TYPE : NULL REMARK 245 REMARK 245 ELECTRON MICROSCOPE SAMPLE REMARK 245 SAMPLE TYPE : PARTICLE REMARK 245 PARTICLE TYPE : POINT REMARK 245 NAME OF SAMPLE : TETHERED AGONIST-BOUND HUMAN REMARK 245 PAR1-GI COMPLEX REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : NULL REMARK 245 SAMPLE SUPPORT DETAILS : NULL REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL REMARK 245 SAMPLE BUFFER : NULL REMARK 245 PH : 7.50 REMARK 245 SAMPLE DETAILS : NULL REMARK 245 REMARK 245 DATA ACQUISITION REMARK 245 DATE OF EXPERIMENT : NULL REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : NULL REMARK 245 TEMPERATURE (KELVIN) : NULL REMARK 245 MICROSCOPE MODEL : FEI TITAN KRIOS REMARK 245 DETECTOR TYPE : GATAN K3 (6K X 4K) REMARK 245 MINIMUM DEFOCUS (NM) : 500.00 REMARK 245 MAXIMUM DEFOCUS (NM) : 2000.00 REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 NOMINAL CS : NULL REMARK 245 IMAGING MODE : BRIGHT FIELD REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 7000.00 REMARK 245 ILLUMINATION MODE : FLOOD BEAM REMARK 245 NOMINAL MAGNIFICATION : NULL REMARK 245 CALIBRATED MAGNIFICATION : NULL REMARK 245 SOURCE : FIELD EMISSION GUN REMARK 245 ACCELERATION VOLTAGE (KV) : 300 REMARK 245 IMAGING DETAILS : NULL REMARK 247 REMARK 247 ELECTRON MICROSCOPY REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION REMARK 247 OF THE STRUCTURE FACTORS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: PENTAMERIC REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: PENTAMERIC REMARK 350 SOFTWARE USED: PISA REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, E, R REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 REMARK 465 M RES C SSSEQI REMARK 465 GLY A 2 REMARK 465 CYS A 3 REMARK 465 THR A 4 REMARK 465 ILE A 56 REMARK 465 HIS A 57 REMARK 465 GLU A 58 REMARK 465 ALA A 59 REMARK 465 GLY A 60 REMARK 465 TYR A 61 REMARK 465 SER A 62 REMARK 465 GLU A 63 REMARK 465 GLU A 64 REMARK 465 GLU A 65 REMARK 465 CYS A 66 REMARK 465 LYS A 67 REMARK 465 GLN A 68 REMARK 465 TYR A 69 REMARK 465 LYS A 70 REMARK 465 ALA A 71 REMARK 465 VAL A 72 REMARK 465 VAL A 73 REMARK 465 TYR A 74 REMARK 465 SER A 75 REMARK 465 ASN A 76 REMARK 465 THR A 77 REMARK 465 ILE A 78 REMARK 465 GLN A 79 REMARK 465 SER A 80 REMARK 465 ILE A 81 REMARK 465 ILE A 82 REMARK 465 ALA A 83 REMARK 465 ILE A 84 REMARK 465 ILE A 85 REMARK 465 ARG A 86 REMARK 465 ALA A 87 REMARK 465 MET A 88 REMARK 465 GLY A 89 REMARK 465 ARG A 90 REMARK 465 LEU A 91 REMARK 465 LYS A 92 REMARK 465 ILE A 93 REMARK 465 ASP A 94 REMARK 465 PHE A 95 REMARK 465 GLY A 96 REMARK 465 ASP A 97 REMARK 465 SER A 98 REMARK 465 ALA A 99 REMARK 465 ARG A 100 REMARK 465 ALA A 101 REMARK 465 ASP A 102 REMARK 465 ASP A 103 REMARK 465 ALA A 104 REMARK 465 ARG A 105 REMARK 465 GLN A 106 REMARK 465 LEU A 107 REMARK 465 PHE A 108 REMARK 465 VAL A 109 REMARK 465 LEU A 110 REMARK 465 ALA A 111 REMARK 465 GLY A 112 REMARK 465 ALA A 113 REMARK 465 ALA A 114 REMARK 465 GLU A 115 REMARK 465 GLU A 116 REMARK 465 GLY A 117 REMARK 465 PHE A 118 REMARK 465 MET A 119 REMARK 465 THR A 120 REMARK 465 ALA A 121 REMARK 465 GLU A 122 REMARK 465 LEU A 123 REMARK 465 ALA A 124 REMARK 465 GLY A 125 REMARK 465 VAL A 126 REMARK 465 ILE A 127 REMARK 465 LYS A 128 REMARK 465 ARG A 129 REMARK 465 LEU A 130 REMARK 465 TRP A 131 REMARK 465 LYS A 132 REMARK 465 ASP A 133 REMARK 465 SER A 134 REMARK 465 GLY A 135 REMARK 465 VAL A 136 REMARK 465 GLN A 137 REMARK 465 ALA A 138 REMARK 465 CYS A 139 REMARK 465 PHE A 140 REMARK 465 ASN A 141 REMARK 465 ARG A 142 REMARK 465 SER A 143 REMARK 465 ARG A 144 REMARK 465 GLU A 145 REMARK 465 TYR A 146 REMARK 465 GLN A 147 REMARK 465 LEU A 148 REMARK 465 ASN A 149 REMARK 465 ASP A 150 REMARK 465 SER A 151 REMARK 465 ALA A 152 REMARK 465 ALA A 153 REMARK 465 TYR A 154 REMARK 465 TYR A 155 REMARK 465 LEU A 156 REMARK 465 ASN A 157 REMARK 465 ASP A 158 REMARK 465 LEU A 159 REMARK 465 ASP A 160 REMARK 465 ARG A 161 REMARK 465 ILE A 162 REMARK 465 ALA A 163 REMARK 465 GLN A 164 REMARK 465 PRO A 165 REMARK 465 ASN A 166 REMARK 465 TYR A 167 REMARK 465 ILE A 168 REMARK 465 PRO A 169 REMARK 465 THR A 170 REMARK 465 GLN A 171 REMARK 465 GLN A 172 REMARK 465 ASP A 173 REMARK 465 VAL A 174 REMARK 465 LEU A 175 REMARK 465 ARG A 176 REMARK 465 THR A 177 REMARK 465 ARG A 178 REMARK 465 VAL A 179 REMARK 465 LYS A 180 REMARK 465 THR A 181 REMARK 465 LEU A 234 REMARK 465 ALA A 235 REMARK 465 GLU A 236 REMARK 465 ASP A 237 REMARK 465 GLU A 238 REMARK 465 GLU A 239 REMARK 465 MET A 240 REMARK 465 MET B -30 REMARK 465 VAL B -29 REMARK 465 SER B -28 REMARK 465 GLY B -27 REMARK 465 TRP B -26 REMARK 465 ARG B -25 REMARK 465 LEU B -24 REMARK 465 PHE B -23 REMARK 465 LYS B -22 REMARK 465 LYS B -21 REMARK 465 ILE B -20 REMARK 465 SER B -19 REMARK 465 GLY B -18 REMARK 465 SER B -17 REMARK 465 SER B -16 REMARK 465 GLY B -15 REMARK 465 GLY B -14 REMARK 465 GLY B -13 REMARK 465 GLY B -12 REMARK 465 SER B -11 REMARK 465 GLY B -10 REMARK 465 GLY B -9 REMARK 465 GLY B -8 REMARK 465 GLY B -7 REMARK 465 SER B -6 REMARK 465 SER B -5 REMARK 465 GLY B -4 REMARK 465 GLY B -3 REMARK 465 SER B -2 REMARK 465 LEU B -1 REMARK 465 LEU B 0 REMARK 465 GLN B 1 REMARK 465 SER B 2 REMARK 465 GLU B 3 REMARK 465 LEU B 4 REMARK 465 HIS B 341 REMARK 465 HIS B 342 REMARK 465 HIS B 343 REMARK 465 HIS B 344 REMARK 465 HIS B 345 REMARK 465 HIS B 346 REMARK 465 HIS B 347 REMARK 465 HIS B 348 REMARK 465 ALA C 2 REMARK 465 SER C 3 REMARK 465 ASN C 4 REMARK 465 ASN C 5 REMARK 465 THR C 6 REMARK 465 ALA C 7 REMARK 465 SER C 8 REMARK 465 GLU C 63 REMARK 465 LYS C 64 REMARK 465 LYS C 65 REMARK 465 PHE C 66 REMARK 465 PHE C 67 REMARK 465 CYS C 68 REMARK 465 LEU E -35 REMARK 465 LEU E -34 REMARK 465 VAL E -33 REMARK 465 ASN E -32 REMARK 465 GLN E -31 REMARK 465 SER E -30 REMARK 465 HIS E -29 REMARK 465 GLN E -28 REMARK 465 GLY E -27 REMARK 465 PHE E -26 REMARK 465 ASN E -25 REMARK 465 LYS E -24 REMARK 465 GLU E -23 REMARK 465 HIS E -22 REMARK 465 THR E -21 REMARK 465 SER E -20 REMARK 465 LYS E -19 REMARK 465 MET E -18 REMARK 465 VAL E -17 REMARK 465 SER E -16 REMARK 465 ALA E -15 REMARK 465 ILE E -14 REMARK 465 VAL E -13 REMARK 465 LEU E -12 REMARK 465 TYR E -11 REMARK 465 VAL E -10 REMARK 465 LEU E -9 REMARK 465 LEU E -8 REMARK 465 ALA E -7 REMARK 465 ALA E -6 REMARK 465 ALA E -5 REMARK 465 ALA E -4 REMARK 465 HIS E -3 REMARK 465 SER E -2 REMARK 465 ALA E -1 REMARK 465 PHE E 0 REMARK 465 ALA E 1 REMARK 465 GLY E 121A REMARK 465 GLY E 121B REMARK 465 GLY E 121C REMARK 465 GLY E 121D REMARK 465 SER E 121E REMARK 465 GLY E 121F REMARK 465 GLY E 121G REMARK 465 GLY E 121H REMARK 465 GLY E 121I REMARK 465 SER E 121J REMARK 465 GLY E 121K REMARK 465 GLY E 121L REMARK 465 GLY E 121M REMARK 465 GLY E 121N REMARK 465 SER E 121O REMARK 465 VAL E 248 REMARK 465 ASP E 249 REMARK 465 GLU E 250 REMARK 465 ASN E 251 REMARK 465 LEU E 252 REMARK 465 TYR E 253 REMARK 465 PHE E 254 REMARK 465 GLN E 255 REMARK 465 GLY E 256 REMARK 465 ALA E 257 REMARK 465 SER E 258 REMARK 465 HIS E 259 REMARK 465 HIS E 260 REMARK 465 HIS E 261 REMARK 465 HIS E 262 REMARK 465 HIS E 263 REMARK 465 HIS E 264 REMARK 465 HIS E 265 REMARK 465 HIS E 266 REMARK 465 TRP E 267 REMARK 465 PHE E 268 REMARK 465 LEU E 269 REMARK 465 GLN E 270 REMARK 465 ARG E 271 REMARK 465 PRO E 272 REMARK 465 GLY E 273 REMARK 465 GLN E 274 REMARK 465 SER E 275 REMARK 465 PRO E 276 REMARK 465 GLN E 277 REMARK 465 LEU E 278 REMARK 465 LEU E 279 REMARK 465 ILE E 280 REMARK 465 TYR E 281 REMARK 465 ARG E 282 REMARK 465 MET E 283 REMARK 465 SER E 284 REMARK 465 ASN E 285 REMARK 465 LEU E 286 REMARK 465 ALA E 287 REMARK 465 SER E 288 REMARK 465 GLY E 289 REMARK 465 VAL E 290 REMARK 465 PRO E 291 REMARK 465 ASP E 292 REMARK 465 ARG E 293 REMARK 465 PHE E 294 REMARK 465 SER E 295 REMARK 465 GLY E 296 REMARK 465 SER E 297 REMARK 465 GLY E 298 REMARK 465 SER E 299 REMARK 465 GLY E 300 REMARK 465 THR E 301 REMARK 465 ALA E 302 REMARK 465 PHE E 303 REMARK 465 THR E 304 REMARK 465 LEU E 305 REMARK 465 THR E 306 REMARK 465 ILE E 307 REMARK 465 SER E 308 REMARK 465 ARG E 309 REMARK 465 LEU E 310 REMARK 465 GLU E 311 REMARK 465 ALA E 312 REMARK 465 GLU E 313 REMARK 465 ASP E 314 REMARK 465 VAL E 315 REMARK 465 GLY E 316 REMARK 465 VAL E 317 REMARK 465 TYR E 318 REMARK 465 TYR E 319 REMARK 465 CYS E 320 REMARK 465 MET E 321 REMARK 465 GLN E 322 REMARK 465 HIS E 323 REMARK 465 LEU E 324 REMARK 465 GLU E 325 REMARK 465 TYR E 326 REMARK 465 PRO E 327 REMARK 465 LEU E 328 REMARK 465 THR E 329 REMARK 465 PHE E 330 REMARK 465 GLY E 331 REMARK 465 ALA E 332 REMARK 465 GLY E 333 REMARK 465 THR E 334 REMARK 465 LYS E 335 REMARK 465 LEU E 336 REMARK 465 GLU E 337 REMARK 465 LEU E 338 REMARK 465 PRO R 48 REMARK 465 ASN R 49 REMARK 465 ASP R 50 REMARK 465 LYS R 51 REMARK 465 TYR R 52 REMARK 465 GLU R 53 REMARK 465 PRO R 54 REMARK 465 PHE R 55 REMARK 465 TRP R 56 REMARK 465 GLU R 57 REMARK 465 ASP R 58 REMARK 465 GLU R 59 REMARK 465 GLU R 60 REMARK 465 LYS R 61 REMARK 465 ASN R 62 REMARK 465 GLU R 63 REMARK 465 SER R 64 REMARK 465 GLY R 65 REMARK 465 LEU R 66 REMARK 465 THR R 67 REMARK 465 GLU R 68 REMARK 465 TYR R 69 REMARK 465 ARG R 70 REMARK 465 LEU R 71 REMARK 465 VAL R 72 REMARK 465 SER R 73 REMARK 465 ILE R 74 REMARK 465 ASN R 75 REMARK 465 LYS R 76 REMARK 465 SER R 77 REMARK 465 SER R 78 REMARK 465 PRO R 79 REMARK 465 LEU R 80 REMARK 465 GLN R 81 REMARK 465 LYS R 82 REMARK 465 GLN R 83 REMARK 465 LEU R 84 REMARK 465 SER R 300 REMARK 465 ALA R 301 REMARK 465 VAL R 302 REMARK 465 ALA R 303 REMARK 465 ARG R 380 REMARK 465 TYR R 381 REMARK 465 VAL R 382 REMARK 465 TYR R 383 REMARK 465 SER R 384 REMARK 465 ILE R 385 REMARK 465 LEU R 386 REMARK 465 CYS R 387 REMARK 465 CYS R 388 REMARK 465 LYS R 389 REMARK 465 GLU R 390 REMARK 465 SER R 391 REMARK 465 SER R 392 REMARK 465 ASP R 393 REMARK 465 PRO R 394 REMARK 465 SER R 395 REMARK 465 SER R 396 REMARK 465 TYR R 397 REMARK 465 GLY R 398 REMARK 465 SER R 399 REMARK 465 SER R 400 REMARK 465 GLY R 401 REMARK 465 GLY R 402 REMARK 465 GLY R 403 REMARK 465 GLY R 404 REMARK 465 SER R 405 REMARK 465 GLY R 406 REMARK 465 GLY R 407 REMARK 465 GLY R 408 REMARK 465 GLY R 409 REMARK 465 SER R 410 REMARK 465 SER R 411 REMARK 465 GLY R 412 REMARK 465 VAL R 413 REMARK 465 PHE R 414 REMARK 465 THR R 415 REMARK 465 LEU R 416 REMARK 465 GLU R 417 REMARK 465 ASP R 418 REMARK 465 PHE R 419 REMARK 465 VAL R 420 REMARK 465 GLY R 421 REMARK 465 ASP R 422 REMARK 465 TRP R 423 REMARK 465 GLU R 424 REMARK 465 GLN R 425 REMARK 465 THR R 426 REMARK 465 ALA R 427 REMARK 465 ALA R 428 REMARK 465 TYR R 429 REMARK 465 ASN R 430 REMARK 465 LEU R 431 REMARK 465 ASP R 432 REMARK 465 GLN R 433 REMARK 465 VAL R 434 REMARK 465 LEU R 435 REMARK 465 GLU R 436 REMARK 465 GLN R 437 REMARK 465 GLY R 438 REMARK 465 GLY R 439 REMARK 465 VAL R 440 REMARK 465 SER R 441 REMARK 465 SER R 442 REMARK 465 LEU R 443 REMARK 465 LEU R 444 REMARK 465 GLN R 445 REMARK 465 ASN R 446 REMARK 465 LEU R 447 REMARK 465 ALA R 448 REMARK 465 VAL R 449 REMARK 465 SER R 450 REMARK 465 VAL R 451 REMARK 465 THR R 452 REMARK 465 PRO R 453 REMARK 465 ILE R 454 REMARK 465 GLN R 455 REMARK 465 ARG R 456 REMARK 465 ILE R 457 REMARK 465 VAL R 458 REMARK 465 ARG R 459 REMARK 465 SER R 460 REMARK 465 GLY R 461 REMARK 465 GLU R 462 REMARK 465 ASN R 463 REMARK 465 ALA R 464 REMARK 465 LEU R 465 REMARK 465 LYS R 466 REMARK 465 ILE R 467 REMARK 465 ASP R 468 REMARK 465 ILE R 469 REMARK 465 HIS R 470 REMARK 465 VAL R 471 REMARK 465 ILE R 472 REMARK 465 ILE R 473 REMARK 465 PRO R 474 REMARK 465 TYR R 475 REMARK 465 GLU R 476 REMARK 465 GLY R 477 REMARK 465 LEU R 478 REMARK 465 SER R 479 REMARK 465 ALA R 480 REMARK 465 ASP R 481 REMARK 465 GLN R 482 REMARK 465 MET R 483 REMARK 465 ALA R 484 REMARK 465 GLN R 485 REMARK 465 ILE R 486 REMARK 465 GLU R 487 REMARK 465 GLU R 488 REMARK 465 VAL R 489 REMARK 465 PHE R 490 REMARK 465 LYS R 491 REMARK 465 VAL R 492 REMARK 465 VAL R 493 REMARK 465 TYR R 494 REMARK 465 PRO R 495 REMARK 465 VAL R 496 REMARK 465 ASP R 497 REMARK 465 ASP R 498 REMARK 465 HIS R 499 REMARK 465 HIS R 500 REMARK 465 PHE R 501 REMARK 465 LYS R 502 REMARK 465 VAL R 503 REMARK 465 ILE R 504 REMARK 465 LEU R 505 REMARK 465 PRO R 506 REMARK 465 TYR R 507 REMARK 465 GLY R 508 REMARK 465 THR R 509 REMARK 465 LEU R 510 REMARK 465 VAL R 511 REMARK 465 ILE R 512 REMARK 465 ASP R 513 REMARK 465 GLY R 514 REMARK 465 VAL R 515 REMARK 465 THR R 516 REMARK 465 PRO R 517 REMARK 465 ASN R 518 REMARK 465 MET R 519 REMARK 465 LEU R 520 REMARK 465 ASN R 521 REMARK 465 TYR R 522 REMARK 465 PHE R 523 REMARK 465 GLY R 524 REMARK 465 ARG R 525 REMARK 465 PRO R 526 REMARK 465 TYR R 527 REMARK 465 GLU R 528 REMARK 465 GLY R 529 REMARK 465 ILE R 530 REMARK 465 ALA R 531 REMARK 465 VAL R 532 REMARK 465 PHE R 533 REMARK 465 ASP R 534 REMARK 465 GLY R 535 REMARK 465 LYS R 536 REMARK 465 LYS R 537 REMARK 465 ILE R 538 REMARK 465 THR R 539 REMARK 465 VAL R 540 REMARK 465 THR R 541 REMARK 465 GLY R 542 REMARK 465 THR R 543 REMARK 465 LEU R 544 REMARK 465 TRP R 545 REMARK 465 ASN R 546 REMARK 465 GLY R 547 REMARK 465 ASN R 548 REMARK 465 LYS R 549 REMARK 465 ILE R 550 REMARK 465 ILE R 551 REMARK 465 ASP R 552 REMARK 465 GLU R 553 REMARK 465 ARG R 554 REMARK 465 LEU R 555 REMARK 465 ILE R 556 REMARK 465 THR R 557 REMARK 465 PRO R 558 REMARK 465 ASP R 559 REMARK 465 GLY R 560 REMARK 465 SER R 561 REMARK 465 MET R 562 REMARK 465 LEU R 563 REMARK 465 PHE R 564 REMARK 465 ARG R 565 REMARK 465 VAL R 566 REMARK 465 THR R 567 REMARK 465 ILE R 568 REMARK 465 ASN R 569 REMARK 465 SER R 570 REMARK 465 GLY R 571 REMARK 465 GLY R 572 REMARK 465 SER R 573 REMARK 470 REMARK 470 MISSING ATOM REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER; REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; REMARK 470 I=INSERTION CODE): REMARK 470 M RES CSSEQI ATOMS REMARK 470 ARG B 251 CG CD NE CZ NH1 NH2 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 GLU A 207 103.80 -59.07 REMARK 500 ASN A 294 37.16 -90.32 REMARK 500 TRP B 99 45.50 -82.15 REMARK 500 THR B 196 11.68 55.89 REMARK 500 THR B 223 10.26 -141.50 REMARK 500 SER B 227 -60.98 -122.40 REMARK 500 ASP B 258 57.30 37.78 REMARK 500 ASP B 291 32.62 -82.13 REMARK 500 PHE C 61 64.07 -110.77 REMARK 500 VAL E 48 -54.60 -120.93 REMARK 500 PHE E 108 19.19 57.37 REMARK 500 ARG E 191 65.60 39.07 REMARK 500 MET E 192 -38.66 71.33 REMARK 500 ASP E 201 3.47 -66.46 REMARK 500 THR E 210 -25.43 -140.60 REMARK 500 LEU E 233 -71.65 -79.75 REMARK 500 LEU R 129 -62.45 -103.15 REMARK 500 TYR R 206 59.55 -119.26 REMARK 500 GLN R 242 45.38 -91.40 REMARK 500 CYS R 254 -101.89 -91.20 REMARK 500 TYR R 266 -59.23 -141.75 REMARK 500 PHE R 279 -58.51 -127.55 REMARK 500 PHE R 326 -32.88 -136.13 REMARK 500 PHE R 339 30.91 -93.21 REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: EMD-38539 RELATED DB: EMDB REMARK 900 CRYO-EM STRUCTURE OF LIGAND-BOUND FORM OF THE RECEPTOR IN COMPLEX REMARK 900 WITH THE TRANSDUCER DBREF 8XOS A 2 354 UNP P63096 GNAI1_HUMAN 2 354 DBREF 8XOS B 2 340 UNP P54311 GBB1_RAT 2 340 DBREF 8XOS C 2 68 UNP P63212 GBG2_BOVIN 2 68 DBREF 8XOS E -35 338 PDB 8XOS 8XOS -35 338 DBREF 8XOS R 42 397 UNP P25116 PAR1_HUMAN 42 397 SEQADV 8XOS ALA A 203 UNP P63096 GLY 203 ENGINEERED MUTATION SEQADV 8XOS SER A 326 UNP P63096 ALA 326 ENGINEERED MUTATION SEQADV 8XOS MET B -30 UNP P54311 INITIATING METHIONINE SEQADV 8XOS VAL B -29 UNP P54311 EXPRESSION TAG SEQADV 8XOS SER B -28 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -27 UNP P54311 EXPRESSION TAG SEQADV 8XOS TRP B -26 UNP P54311 EXPRESSION TAG SEQADV 8XOS ARG B -25 UNP P54311 EXPRESSION TAG SEQADV 8XOS LEU B -24 UNP P54311 EXPRESSION TAG SEQADV 8XOS PHE B -23 UNP P54311 EXPRESSION TAG SEQADV 8XOS LYS B -22 UNP P54311 EXPRESSION TAG SEQADV 8XOS LYS B -21 UNP P54311 EXPRESSION TAG SEQADV 8XOS ILE B -20 UNP P54311 EXPRESSION TAG SEQADV 8XOS SER B -19 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -18 UNP P54311 EXPRESSION TAG SEQADV 8XOS SER B -17 UNP P54311 EXPRESSION TAG SEQADV 8XOS SER B -16 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -15 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -14 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -13 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -12 UNP P54311 EXPRESSION TAG SEQADV 8XOS SER B -11 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -10 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -9 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -8 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -7 UNP P54311 EXPRESSION TAG SEQADV 8XOS SER B -6 UNP P54311 EXPRESSION TAG SEQADV 8XOS SER B -5 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -4 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY B -3 UNP P54311 EXPRESSION TAG SEQADV 8XOS SER B -2 UNP P54311 EXPRESSION TAG SEQADV 8XOS LEU B -1 UNP P54311 EXPRESSION TAG SEQADV 8XOS LEU B 0 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLN B 1 UNP P54311 EXPRESSION TAG SEQADV 8XOS HIS B 341 UNP P54311 EXPRESSION TAG SEQADV 8XOS HIS B 342 UNP P54311 EXPRESSION TAG SEQADV 8XOS HIS B 343 UNP P54311 EXPRESSION TAG SEQADV 8XOS HIS B 344 UNP P54311 EXPRESSION TAG SEQADV 8XOS HIS B 345 UNP P54311 EXPRESSION TAG SEQADV 8XOS HIS B 346 UNP P54311 EXPRESSION TAG SEQADV 8XOS HIS B 347 UNP P54311 EXPRESSION TAG SEQADV 8XOS HIS B 348 UNP P54311 EXPRESSION TAG SEQADV 8XOS GLY R 398 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 399 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 400 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 401 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 402 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 403 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 404 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 405 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 406 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 407 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 408 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 409 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 410 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 411 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 412 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 413 UNP P25116 EXPRESSION TAG SEQADV 8XOS PHE R 414 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 415 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 416 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 417 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 418 UNP P25116 EXPRESSION TAG SEQADV 8XOS PHE R 419 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 420 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 421 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 422 UNP P25116 EXPRESSION TAG SEQADV 8XOS TRP R 423 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 424 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLN R 425 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 426 UNP P25116 EXPRESSION TAG SEQADV 8XOS ALA R 427 UNP P25116 EXPRESSION TAG SEQADV 8XOS ALA R 428 UNP P25116 EXPRESSION TAG SEQADV 8XOS TYR R 429 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASN R 430 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 431 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 432 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLN R 433 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 434 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 435 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 436 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLN R 437 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 438 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 439 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 440 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 441 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 442 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 443 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 444 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLN R 445 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASN R 446 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 447 UNP P25116 EXPRESSION TAG SEQADV 8XOS ALA R 448 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 449 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 450 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 451 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 452 UNP P25116 EXPRESSION TAG SEQADV 8XOS PRO R 453 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 454 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLN R 455 UNP P25116 EXPRESSION TAG SEQADV 8XOS ARG R 456 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 457 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 458 UNP P25116 EXPRESSION TAG SEQADV 8XOS ARG R 459 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 460 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 461 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 462 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASN R 463 UNP P25116 EXPRESSION TAG SEQADV 8XOS ALA R 464 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 465 UNP P25116 EXPRESSION TAG SEQADV 8XOS LYS R 466 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 467 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 468 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 469 UNP P25116 EXPRESSION TAG SEQADV 8XOS HIS R 470 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 471 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 472 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 473 UNP P25116 EXPRESSION TAG SEQADV 8XOS PRO R 474 UNP P25116 EXPRESSION TAG SEQADV 8XOS TYR R 475 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 476 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 477 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 478 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 479 UNP P25116 EXPRESSION TAG SEQADV 8XOS ALA R 480 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 481 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLN R 482 UNP P25116 EXPRESSION TAG SEQADV 8XOS MET R 483 UNP P25116 EXPRESSION TAG SEQADV 8XOS ALA R 484 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLN R 485 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 486 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 487 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 488 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 489 UNP P25116 EXPRESSION TAG SEQADV 8XOS PHE R 490 UNP P25116 EXPRESSION TAG SEQADV 8XOS LYS R 491 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 492 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 493 UNP P25116 EXPRESSION TAG SEQADV 8XOS TYR R 494 UNP P25116 EXPRESSION TAG SEQADV 8XOS PRO R 495 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 496 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 497 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 498 UNP P25116 EXPRESSION TAG SEQADV 8XOS HIS R 499 UNP P25116 EXPRESSION TAG SEQADV 8XOS HIS R 500 UNP P25116 EXPRESSION TAG SEQADV 8XOS PHE R 501 UNP P25116 EXPRESSION TAG SEQADV 8XOS LYS R 502 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 503 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 504 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 505 UNP P25116 EXPRESSION TAG SEQADV 8XOS PRO R 506 UNP P25116 EXPRESSION TAG SEQADV 8XOS TYR R 507 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 508 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 509 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 510 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 511 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 512 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 513 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 514 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 515 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 516 UNP P25116 EXPRESSION TAG SEQADV 8XOS PRO R 517 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASN R 518 UNP P25116 EXPRESSION TAG SEQADV 8XOS MET R 519 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 520 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASN R 521 UNP P25116 EXPRESSION TAG SEQADV 8XOS TYR R 522 UNP P25116 EXPRESSION TAG SEQADV 8XOS PHE R 523 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 524 UNP P25116 EXPRESSION TAG SEQADV 8XOS ARG R 525 UNP P25116 EXPRESSION TAG SEQADV 8XOS PRO R 526 UNP P25116 EXPRESSION TAG SEQADV 8XOS TYR R 527 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 528 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 529 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 530 UNP P25116 EXPRESSION TAG SEQADV 8XOS ALA R 531 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 532 UNP P25116 EXPRESSION TAG SEQADV 8XOS PHE R 533 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 534 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 535 UNP P25116 EXPRESSION TAG SEQADV 8XOS LYS R 536 UNP P25116 EXPRESSION TAG SEQADV 8XOS LYS R 537 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 538 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 539 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 540 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 541 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 542 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 543 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 544 UNP P25116 EXPRESSION TAG SEQADV 8XOS TRP R 545 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASN R 546 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 547 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASN R 548 UNP P25116 EXPRESSION TAG SEQADV 8XOS LYS R 549 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 550 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 551 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 552 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLU R 553 UNP P25116 EXPRESSION TAG SEQADV 8XOS ARG R 554 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 555 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 556 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 557 UNP P25116 EXPRESSION TAG SEQADV 8XOS PRO R 558 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASP R 559 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 560 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 561 UNP P25116 EXPRESSION TAG SEQADV 8XOS MET R 562 UNP P25116 EXPRESSION TAG SEQADV 8XOS LEU R 563 UNP P25116 EXPRESSION TAG SEQADV 8XOS PHE R 564 UNP P25116 EXPRESSION TAG SEQADV 8XOS ARG R 565 UNP P25116 EXPRESSION TAG SEQADV 8XOS VAL R 566 UNP P25116 EXPRESSION TAG SEQADV 8XOS THR R 567 UNP P25116 EXPRESSION TAG SEQADV 8XOS ILE R 568 UNP P25116 EXPRESSION TAG SEQADV 8XOS ASN R 569 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 570 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 571 UNP P25116 EXPRESSION TAG SEQADV 8XOS GLY R 572 UNP P25116 EXPRESSION TAG SEQADV 8XOS SER R 573 UNP P25116 EXPRESSION TAG SEQRES 1 A 353 GLY CYS THR LEU SER ALA GLU ASP LYS ALA ALA VAL GLU SEQRES 2 A 353 ARG SER LYS MET ILE ASP ARG ASN LEU ARG GLU ASP GLY SEQRES 3 A 353 GLU LYS ALA ALA ARG GLU VAL LYS LEU LEU LEU LEU GLY SEQRES 4 A 353 ALA GLY GLU SER GLY LYS SER THR ILE VAL LYS GLN MET SEQRES 5 A 353 LYS ILE ILE HIS GLU ALA GLY TYR SER GLU GLU GLU CYS SEQRES 6 A 353 LYS GLN TYR LYS ALA VAL VAL TYR SER ASN THR ILE GLN SEQRES 7 A 353 SER ILE ILE ALA ILE ILE ARG ALA MET GLY ARG LEU LYS SEQRES 8 A 353 ILE ASP PHE GLY ASP SER ALA ARG ALA ASP ASP ALA ARG SEQRES 9 A 353 GLN LEU PHE VAL LEU ALA GLY ALA ALA GLU GLU GLY PHE SEQRES 10 A 353 MET THR ALA GLU LEU ALA GLY VAL ILE LYS ARG LEU TRP SEQRES 11 A 353 LYS ASP SER GLY VAL GLN ALA CYS PHE ASN ARG SER ARG SEQRES 12 A 353 GLU TYR GLN LEU ASN ASP SER ALA ALA TYR TYR LEU ASN SEQRES 13 A 353 ASP LEU ASP ARG ILE ALA GLN PRO ASN TYR ILE PRO THR SEQRES 14 A 353 GLN GLN ASP VAL LEU ARG THR ARG VAL LYS THR THR GLY SEQRES 15 A 353 ILE VAL GLU THR HIS PHE THR PHE LYS ASP LEU HIS PHE SEQRES 16 A 353 LYS MET PHE ASP VAL GLY ALA GLN ARG SER GLU ARG LYS SEQRES 17 A 353 LYS TRP ILE HIS CYS PHE GLU GLY VAL THR ALA ILE ILE SEQRES 18 A 353 PHE CYS VAL ALA LEU SER ASP TYR ASP LEU VAL LEU ALA SEQRES 19 A 353 GLU ASP GLU GLU MET ASN ARG MET HIS GLU SER MET LYS SEQRES 20 A 353 LEU PHE ASP SER ILE CYS ASN ASN LYS TRP PHE THR ASP SEQRES 21 A 353 THR SER ILE ILE LEU PHE LEU ASN LYS LYS ASP LEU PHE SEQRES 22 A 353 GLU GLU LYS ILE LYS LYS SER PRO LEU THR ILE CYS TYR SEQRES 23 A 353 PRO GLU TYR ALA GLY SER ASN THR TYR GLU GLU ALA ALA SEQRES 24 A 353 ALA TYR ILE GLN CYS GLN PHE GLU ASP LEU ASN LYS ARG SEQRES 25 A 353 LYS ASP THR LYS GLU ILE TYR THR HIS PHE THR CYS SER SEQRES 26 A 353 THR ASP THR LYS ASN VAL GLN PHE VAL PHE ASP ALA VAL SEQRES 27 A 353 THR ASP VAL ILE ILE LYS ASN ASN LEU LYS ASP CYS GLY SEQRES 28 A 353 LEU PHE SEQRES 1 B 379 MET VAL SER GLY TRP ARG LEU PHE LYS LYS ILE SER GLY SEQRES 2 B 379 SER SER GLY GLY GLY GLY SER GLY GLY GLY GLY SER SER SEQRES 3 B 379 GLY GLY SER LEU LEU GLN SER GLU LEU ASP GLN LEU ARG SEQRES 4 B 379 GLN GLU ALA GLU GLN LEU LYS ASN GLN ILE ARG ASP ALA SEQRES 5 B 379 ARG LYS ALA CYS ALA ASP ALA THR LEU SER GLN ILE THR SEQRES 6 B 379 ASN ASN ILE ASP PRO VAL GLY ARG ILE GLN MET ARG THR SEQRES 7 B 379 ARG ARG THR LEU ARG GLY HIS LEU ALA LYS ILE TYR ALA SEQRES 8 B 379 MET HIS TRP GLY THR ASP SER ARG LEU LEU VAL SER ALA SEQRES 9 B 379 SER GLN ASP GLY LYS LEU ILE ILE TRP ASP SER TYR THR SEQRES 10 B 379 THR ASN LYS VAL HIS ALA ILE PRO LEU ARG SER SER TRP SEQRES 11 B 379 VAL MET THR CYS ALA TYR ALA PRO SER GLY ASN TYR VAL SEQRES 12 B 379 ALA CYS GLY GLY LEU ASP ASN ILE CYS SER ILE TYR ASN SEQRES 13 B 379 LEU LYS THR ARG GLU GLY ASN VAL ARG VAL SER ARG GLU SEQRES 14 B 379 LEU ALA GLY HIS THR GLY TYR LEU SER CYS CYS ARG PHE SEQRES 15 B 379 LEU ASP ASP ASN GLN ILE VAL THR SER SER GLY ASP THR SEQRES 16 B 379 THR CYS ALA LEU TRP ASP ILE GLU THR GLY GLN GLN THR SEQRES 17 B 379 THR THR PHE THR GLY HIS THR GLY ASP VAL MET SER LEU SEQRES 18 B 379 SER LEU ALA PRO ASP THR ARG LEU PHE VAL SER GLY ALA SEQRES 19 B 379 CYS ASP ALA SER ALA LYS LEU TRP ASP VAL ARG GLU GLY SEQRES 20 B 379 MET CYS ARG GLN THR PHE THR GLY HIS GLU SER ASP ILE SEQRES 21 B 379 ASN ALA ILE CYS PHE PHE PRO ASN GLY ASN ALA PHE ALA SEQRES 22 B 379 THR GLY SER ASP ASP ALA THR CYS ARG LEU PHE ASP LEU SEQRES 23 B 379 ARG ALA ASP GLN GLU LEU MET THR TYR SER HIS ASP ASN SEQRES 24 B 379 ILE ILE CYS GLY ILE THR SER VAL SER PHE SER LYS SER SEQRES 25 B 379 GLY ARG LEU LEU LEU ALA GLY TYR ASP ASP PHE ASN CYS SEQRES 26 B 379 ASN VAL TRP ASP ALA LEU LYS ALA ASP ARG ALA GLY VAL SEQRES 27 B 379 LEU ALA GLY HIS ASP ASN ARG VAL SER CYS LEU GLY VAL SEQRES 28 B 379 THR ASP ASP GLY MET ALA VAL ALA THR GLY SER TRP ASP SEQRES 29 B 379 SER PHE LEU LYS ILE TRP ASN HIS HIS HIS HIS HIS HIS SEQRES 30 B 379 HIS HIS SEQRES 1 C 67 ALA SER ASN ASN THR ALA SER ILE ALA GLN ALA ARG LYS SEQRES 2 C 67 LEU VAL GLU GLN LEU LYS MET GLU ALA ASN ILE ASP ARG SEQRES 3 C 67 ILE LYS VAL SER LYS ALA ALA ALA ASP LEU MET ALA TYR SEQRES 4 C 67 CYS GLU ALA HIS ALA LYS GLU ASP PRO LEU LEU THR PRO SEQRES 5 C 67 VAL PRO ALA SER GLU ASN PRO PHE ARG GLU LYS LYS PHE SEQRES 6 C 67 PHE CYS SEQRES 1 E 375 LEU LEU VAL ASN GLN SER HIS GLN GLY PHE ASN LYS GLU SEQRES 2 E 375 HIS THR SER LYS MET VAL SER ALA ILE VAL LEU TYR VAL SEQRES 3 E 375 LEU LEU ALA ALA ALA ALA HIS SER ALA PHE ALA VAL GLN SEQRES 4 E 375 LEU VAL GLU SER GLY GLY GLY LEU VAL GLN PRO GLY GLY SEQRES 5 E 375 SER ARG LYS LEU SER CYS SER ALA SER GLY PHE ALA PHE SEQRES 6 E 375 SER SER PHE GLY MET HIS TRP VAL ARG GLN ALA PRO GLU SEQRES 7 E 375 LYS GLY LEU GLU TRP VAL ALA TYR ILE SER SER GLY SER SEQRES 8 E 375 GLY THR ILE TYR TYR ALA ASP THR VAL LYS GLY ARG PHE SEQRES 9 E 375 THR ILE SER ARG ASP ASP PRO LYS ASN THR LEU PHE LEU SEQRES 10 E 375 GLN MET THR SER LEU ARG SER GLU ASP THR ALA MET TYR SEQRES 11 E 375 TYR CYS VAL ARG SER ILE TYR TYR TYR GLY SER SER PRO SEQRES 12 E 375 PHE ASP PHE TRP GLY GLN GLY THR THR LEU THR VAL SER SEQRES 13 E 375 ALA GLY GLY GLY GLY SER GLY GLY GLY GLY SER GLY GLY SEQRES 14 E 375 GLY GLY SER ALA ASP ILE VAL MET THR GLN ALA THR SER SEQRES 15 E 375 SER VAL PRO VAL THR PRO GLY GLU SER VAL SER ILE SER SEQRES 16 E 375 CYS ARG SER SER LYS SER LEU LEU HIS SER ASN GLY ASN SEQRES 17 E 375 THR TYR LEU TYR TRP PHE LEU GLN ARG PRO GLY GLN SER SEQRES 18 E 375 PRO GLN LEU LEU ILE TYR ARG MET SER ASN LEU ALA SER SEQRES 19 E 375 GLY VAL PRO ASP ARG PHE SER GLY SER GLY SER GLY THR SEQRES 20 E 375 ALA PHE THR LEU THR ILE SER ARG LEU GLU ALA GLU ASP SEQRES 21 E 375 VAL GLY VAL TYR TYR CYS MET GLN HIS LEU GLU TYR PRO SEQRES 22 E 375 LEU THR PHE GLY ALA GLY THR LYS LEU GLU LEU VAL ASP SEQRES 23 E 375 GLU ASN LEU TYR PHE GLN GLY ALA SER HIS HIS HIS HIS SEQRES 24 E 375 HIS HIS HIS HIS TRP PHE LEU GLN ARG PRO GLY GLN SER SEQRES 25 E 375 PRO GLN LEU LEU ILE TYR ARG MET SER ASN LEU ALA SER SEQRES 26 E 375 GLY VAL PRO ASP ARG PHE SER GLY SER GLY SER GLY THR SEQRES 27 E 375 ALA PHE THR LEU THR ILE SER ARG LEU GLU ALA GLU ASP SEQRES 28 E 375 VAL GLY VAL TYR TYR CYS MET GLN HIS LEU GLU TYR PRO SEQRES 29 E 375 LEU THR PHE GLY ALA GLY THR LYS LEU GLU LEU SEQRES 1 R 532 SER PHE LEU LEU ARG ASN PRO ASN ASP LYS TYR GLU PRO SEQRES 2 R 532 PHE TRP GLU ASP GLU GLU LYS ASN GLU SER GLY LEU THR SEQRES 3 R 532 GLU TYR ARG LEU VAL SER ILE ASN LYS SER SER PRO LEU SEQRES 4 R 532 GLN LYS GLN LEU PRO ALA PHE ILE SER GLU ASP ALA SER SEQRES 5 R 532 GLY TYR LEU THR SER SER TRP LEU THR LEU PHE VAL PRO SEQRES 6 R 532 SER VAL TYR THR GLY VAL PHE VAL VAL SER LEU PRO LEU SEQRES 7 R 532 ASN ILE MET ALA ILE VAL VAL PHE ILE LEU LYS MET LYS SEQRES 8 R 532 VAL LYS LYS PRO ALA VAL VAL TYR MET LEU HIS LEU ALA SEQRES 9 R 532 THR ALA ASP VAL LEU PHE VAL SER VAL LEU PRO PHE LYS SEQRES 10 R 532 ILE SER TYR TYR PHE SER GLY SER ASP TRP GLN PHE GLY SEQRES 11 R 532 SER GLU LEU CYS ARG PHE VAL THR ALA ALA PHE TYR CYS SEQRES 12 R 532 ASN MET TYR ALA SER ILE LEU LEU MET THR VAL ILE SER SEQRES 13 R 532 ILE ASP ARG PHE LEU ALA VAL VAL TYR PRO MET GLN SER SEQRES 14 R 532 LEU SER TRP ARG THR LEU GLY ARG ALA SER PHE THR CYS SEQRES 15 R 532 LEU ALA ILE TRP ALA LEU ALA ILE ALA GLY VAL VAL PRO SEQRES 16 R 532 LEU LEU LEU LYS GLU GLN THR ILE GLN VAL PRO GLY LEU SEQRES 17 R 532 ASN ILE THR THR CYS HIS ASP VAL LEU ASN GLU THR LEU SEQRES 18 R 532 LEU GLU GLY TYR TYR ALA TYR TYR PHE SER ALA PHE SER SEQRES 19 R 532 ALA VAL PHE PHE PHE VAL PRO LEU ILE ILE SER THR VAL SEQRES 20 R 532 CYS TYR VAL SER ILE ILE ARG CYS LEU SER SER SER ALA SEQRES 21 R 532 VAL ALA ASN ARG SER LYS LYS SER ARG ALA LEU PHE LEU SEQRES 22 R 532 SER ALA ALA VAL PHE CYS ILE PHE ILE ILE CYS PHE GLY SEQRES 23 R 532 PRO THR ASN VAL LEU LEU ILE ALA HIS TYR SER PHE LEU SEQRES 24 R 532 SER HIS THR SER THR THR GLU ALA ALA TYR PHE ALA TYR SEQRES 25 R 532 LEU LEU CYS VAL CYS VAL SER SER ILE SER CYS CYS ILE SEQRES 26 R 532 ASP PRO LEU ILE TYR TYR TYR ALA SER SER GLU CYS GLN SEQRES 27 R 532 ARG TYR VAL TYR SER ILE LEU CYS CYS LYS GLU SER SER SEQRES 28 R 532 ASP PRO SER SER TYR GLY SER SER GLY GLY GLY GLY SER SEQRES 29 R 532 GLY GLY GLY GLY SER SER GLY VAL PHE THR LEU GLU ASP SEQRES 30 R 532 PHE VAL GLY ASP TRP GLU GLN THR ALA ALA TYR ASN LEU SEQRES 31 R 532 ASP GLN VAL LEU GLU GLN GLY GLY VAL SER SER LEU LEU SEQRES 32 R 532 GLN ASN LEU ALA VAL SER VAL THR PRO ILE GLN ARG ILE SEQRES 33 R 532 VAL ARG SER GLY GLU ASN ALA LEU LYS ILE ASP ILE HIS SEQRES 34 R 532 VAL ILE ILE PRO TYR GLU GLY LEU SER ALA ASP GLN MET SEQRES 35 R 532 ALA GLN ILE GLU GLU VAL PHE LYS VAL VAL TYR PRO VAL SEQRES 36 R 532 ASP ASP HIS HIS PHE LYS VAL ILE LEU PRO TYR GLY THR SEQRES 37 R 532 LEU VAL ILE ASP GLY VAL THR PRO ASN MET LEU ASN TYR SEQRES 38 R 532 PHE GLY ARG PRO TYR GLU GLY ILE ALA VAL PHE ASP GLY SEQRES 39 R 532 LYS LYS ILE THR VAL THR GLY THR LEU TRP ASN GLY ASN SEQRES 40 R 532 LYS ILE ILE ASP GLU ARG LEU ILE THR PRO ASP GLY SER SEQRES 41 R 532 MET LEU PHE ARG VAL THR ILE ASN SER GLY GLY SER HET CLR R 601 28 HET CLR R 602 28 HETNAM CLR CHOLESTEROL FORMUL 6 CLR 2(C27 H46 O) HELIX 1 AA1 SER A 6 ARG A 32 1 27 HELIX 2 AA2 THR A 48 GLN A 52 5 5 HELIX 3 AA3 GLU A 207 VAL A 218 5 12 HELIX 4 AA4 SER A 228 TYR A 230 5 3 HELIX 5 AA5 ARG A 242 ASN A 255 1 14 HELIX 6 AA6 ASN A 256 THR A 260 5 5 HELIX 7 AA7 LYS A 270 SER A 281 1 12 HELIX 8 AA8 PRO A 282 CYS A 286 5 5 HELIX 9 AA9 THR A 295 ASP A 309 1 15 HELIX 10 AB1 THR A 329 CYS A 351 1 23 HELIX 11 AB2 LEU B 7 ALA B 26 1 20 HELIX 12 AB3 THR B 29 ASN B 35 1 7 HELIX 13 AB4 ALA C 10 ASN C 24 1 15 HELIX 14 AB5 LYS C 29 HIS C 44 1 16 HELIX 15 AB6 ALA C 45 ASP C 48 5 4 HELIX 16 AB7 ASP E 74 LYS E 76 5 3 HELIX 17 AB8 ARG E 87 THR E 91 5 5 HELIX 18 AB9 GLU E 220 VAL E 224 5 5 HELIX 19 AC1 SER R 89 SER R 98 1 10 HELIX 20 AC2 LEU R 103 PHE R 127 1 25 HELIX 21 AC3 LYS R 135 VAL R 154 1 20 HELIX 22 AC4 VAL R 154 SER R 164 1 11 HELIX 23 AC5 GLY R 171 TYR R 206 1 36 HELIX 24 AC6 TYR R 206 ARG R 214 1 9 HELIX 25 AC7 THR R 215 VAL R 234 1 20 HELIX 26 AC8 VAL R 234 LYS R 240 1 7 HELIX 27 AC9 GLU R 260 TYR R 266 1 7 HELIX 28 AD1 TYR R 266 PHE R 279 1 14 HELIX 29 AD2 PHE R 279 SER R 298 1 20 HELIX 30 AD3 LYS R 308 CYS R 325 1 18 HELIX 31 AD4 PHE R 326 TYR R 337 1 12 HELIX 32 AD5 HIS R 342 SER R 361 1 20 HELIX 33 AD6 ILE R 362 TYR R 372 1 11 SHEET 1 AA1 6 VAL A 185 PHE A 191 0 SHEET 2 AA1 6 LEU A 194 ASP A 200 -1 O PHE A 196 N PHE A 189 SHEET 3 AA1 6 GLU A 33 GLY A 40 1 N LEU A 36 O LYS A 197 SHEET 4 AA1 6 ALA A 220 ALA A 226 1 O ILE A 222 N LEU A 37 SHEET 5 AA1 6 SER A 263 ASN A 269 1 O ASN A 269 N VAL A 225 SHEET 6 AA1 6 ILE A 319 PHE A 323 1 O TYR A 320 N ILE A 264 SHEET 1 AA2 4 THR B 47 LEU B 51 0 SHEET 2 AA2 4 LEU B 336 TRP B 339 -1 O LEU B 336 N LEU B 51 SHEET 3 AA2 4 VAL B 327 GLY B 330 -1 N VAL B 327 O TRP B 339 SHEET 4 AA2 4 CYS B 317 VAL B 320 -1 N CYS B 317 O GLY B 330 SHEET 1 AA3 4 ILE B 58 TRP B 63 0 SHEET 2 AA3 4 LEU B 69 SER B 74 -1 O ALA B 73 N ALA B 60 SHEET 3 AA3 4 LYS B 78 ASP B 83 -1 O TRP B 82 N LEU B 70 SHEET 4 AA3 4 LYS B 89 PRO B 94 -1 O VAL B 90 N ILE B 81 SHEET 1 AA4 4 VAL B 100 TYR B 105 0 SHEET 2 AA4 4 TYR B 111 GLY B 116 -1 O GLY B 115 N MET B 101 SHEET 3 AA4 4 CYS B 121 ASN B 125 -1 O TYR B 124 N VAL B 112 SHEET 4 AA4 4 ARG B 134 LEU B 139 -1 O LEU B 139 N CYS B 121 SHEET 1 AA5 4 LEU B 146 ASP B 153 0 SHEET 2 AA5 4 GLN B 156 SER B 161 -1 O GLN B 156 N LEU B 152 SHEET 3 AA5 4 CYS B 166 ASP B 170 -1 O TRP B 169 N ILE B 157 SHEET 4 AA5 4 GLN B 175 PHE B 180 -1 O PHE B 180 N CYS B 166 SHEET 1 AA6 4 VAL B 187 LEU B 192 0 SHEET 2 AA6 4 LEU B 198 ALA B 203 -1 O VAL B 200 N SER B 191 SHEET 3 AA6 4 ALA B 208 ASP B 212 -1 O TRP B 211 N PHE B 199 SHEET 4 AA6 4 MET B 217 PHE B 222 -1 O ARG B 219 N LEU B 210 SHEET 1 AA7 4 ILE B 229 PHE B 234 0 SHEET 2 AA7 4 ALA B 240 SER B 245 -1 O GLY B 244 N ALA B 231 SHEET 3 AA7 4 CYS B 250 ASP B 254 -1 O PHE B 253 N PHE B 241 SHEET 4 AA7 4 GLN B 259 TYR B 264 -1 O LEU B 261 N LEU B 252 SHEET 1 AA8 4 ILE B 273 PHE B 278 0 SHEET 2 AA8 4 LEU B 284 TYR B 289 -1 O LEU B 286 N SER B 277 SHEET 3 AA8 4 CYS B 294 ASP B 298 -1 O TRP B 297 N LEU B 285 SHEET 4 AA8 4 ARG B 304 LEU B 308 -1 O LEU B 308 N CYS B 294 SHEET 1 AA9 4 GLN E 3 SER E 7 0 SHEET 2 AA9 4 SER E 17 SER E 25 -1 O SER E 21 N SER E 7 SHEET 3 AA9 4 THR E 78 THR E 84 -1 O LEU E 79 N CYS E 22 SHEET 4 AA9 4 PHE E 68 ASP E 73 -1 N SER E 71 O PHE E 80 SHEET 1 AB1 6 GLY E 10 VAL E 12 0 SHEET 2 AB1 6 THR E 115 VAL E 119 1 O THR E 118 N GLY E 10 SHEET 3 AB1 6 ALA E 92 SER E 99 -1 N TYR E 94 O THR E 115 SHEET 4 AB1 6 GLY E 33 GLN E 39 -1 N VAL E 37 O TYR E 95 SHEET 5 AB1 6 LEU E 45 ILE E 51 -1 O GLU E 46 N ARG E 38 SHEET 6 AB1 6 ILE E 58 TYR E 60 -1 O TYR E 59 N TYR E 50 SHEET 1 AB2 4 GLY E 10 VAL E 12 0 SHEET 2 AB2 4 THR E 115 VAL E 119 1 O THR E 118 N GLY E 10 SHEET 3 AB2 4 ALA E 92 SER E 99 -1 N TYR E 94 O THR E 115 SHEET 4 AB2 4 PHE E 110 TRP E 111 -1 O PHE E 110 N ARG E 98 SHEET 1 AB3 4 MET E 140 GLN E 142 0 SHEET 2 AB3 4 VAL E 155 SER E 161 -1 O ARG E 160 N THR E 141 SHEET 3 AB3 4 ALA E 211 ILE E 216 -1 O LEU E 214 N ILE E 157 SHEET 4 AB3 4 PHE E 203 GLY E 207 -1 N SER E 204 O THR E 215 SHEET 1 AB4 6 SER E 146 PRO E 148 0 SHEET 2 AB4 6 THR E 243 GLU E 246 1 O GLU E 246 N VAL E 147 SHEET 3 AB4 6 VAL E 226 GLN E 231 -1 N TYR E 227 O THR E 243 SHEET 4 AB4 6 LEU E 174 GLN E 179 -1 N TYR E 175 O MET E 230 SHEET 5 AB4 6 GLN E 186 TYR E 190 -1 O LEU E 188 N TRP E 176 SHEET 6 AB4 6 ASN E 194 LEU E 195 -1 O ASN E 194 N TYR E 190 SHEET 1 AB5 3 PHE R 87 ILE R 88 0 SHEET 2 AB5 3 PHE R 43 ARG R 46 -1 N ARG R 46 O PHE R 87 SHEET 3 AB5 3 VAL R 257 ASN R 259 1 O LEU R 258 N LEU R 45 SSBOND 1 CYS R 175 CYS R 254 1555 1555 2.03 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000