HEADER SIGNALING PROTEIN 19-APR-24 8Z74 TITLE CRYO-EM STRUCTURE OF APJR COMPLEX WITH AGONISTIC ANTIBODY COMPND MOL_ID: 1; COMPND 2 MOLECULE: AGONISTIC ANTIBODY; COMPND 3 CHAIN: A, B; COMPND 4 ENGINEERED: YES; COMPND 5 MOL_ID: 2; COMPND 6 MOLECULE: SOLUBLE CYTOCHROME B562,APELIN RECEPTOR; COMPND 7 CHAIN: C, D; COMPND 8 SYNONYM: CYTOCHROME B-562,ANGIOTENSIN RECEPTOR-LIKE 1,G-PROTEIN COMPND 9 COUPLED RECEPTOR APJ,G-PROTEIN COUPLED RECEPTOR HG11; COMPND 10 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_COMMON: HUMAN; SOURCE 4 ORGANISM_TAXID: 9606; SOURCE 5 EXPRESSION_SYSTEM: TRICHOPLUSIA NI; SOURCE 6 EXPRESSION_SYSTEM_TAXID: 7111; SOURCE 7 MOL_ID: 2; SOURCE 8 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 9 ORGANISM_COMMON: HUMAN; SOURCE 10 ORGANISM_TAXID: 9606; SOURCE 11 GENE: CYBC, APLNR, AGTRL1, APJ; SOURCE 12 EXPRESSION_SYSTEM: TRICHOPLUSIA NI; SOURCE 13 EXPRESSION_SYSTEM_TAXID: 7111 KEYWDS GPCR, APJ, AGONISTIC ANTIBODY, SIGNALING PROTEIN EXPDTA ELECTRON MICROSCOPY AUTHOR Y.YUE,L.E.LIU,L.J.WU,F.XU REVDAT 1 22-JAN-25 8Z74 0 JRNL AUTH Y.YUE,L.LIU,L.WU,C.XU,M.NA,S.LIU,Y.LIU,F.LI,J.LIU,S.SHI, JRNL AUTH 2 H.LEI,M.ZHAO,T.YANG,W.JI,A.WANG,M.A.HANSON,R.C.STEVENS, JRNL AUTH 3 J.LIU,F.XU JRNL TITL STRUCTURAL INSIGHTS INTO THE REGULATION OF MONOMERIC AND JRNL TITL 2 DIMERIC APELIN RECEPTOR. JRNL REF NAT COMMUN V. 16 310 2025 JRNL REFN ESSN 2041-1723 JRNL PMID 39747115 JRNL DOI 10.1038/S41467-024-55555-6 REMARK 2 REMARK 2 RESOLUTION. 3.01 ANGSTROMS. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 SOFTWARE PACKAGES : NULL REMARK 3 RECONSTRUCTION SCHEMA : NULL REMARK 3 REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT REMARK 3 PDB ENTRY : NULL REMARK 3 REFINEMENT SPACE : NULL REMARK 3 REFINEMENT PROTOCOL : NULL REMARK 3 REFINEMENT TARGET : NULL REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL REMARK 3 REMARK 3 FITTING PROCEDURE : NULL REMARK 3 REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 3.010 REMARK 3 NUMBER OF PARTICLES : 488065 REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING ONLY REMARK 3 REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL REMARK 3 REMARK 3 OTHER DETAILS: NULL REMARK 4 REMARK 4 8Z74 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 22-APR-24. REMARK 100 THE DEPOSITION ID IS D_1300047148. REMARK 245 REMARK 245 EXPERIMENTAL DETAILS REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE REMARK 245 SPECIMEN TYPE : NULL REMARK 245 REMARK 245 ELECTRON MICROSCOPE SAMPLE REMARK 245 SAMPLE TYPE : PARTICLE REMARK 245 PARTICLE TYPE : POINT REMARK 245 NAME OF SAMPLE : APJR COMPLEX WITH ANTIBODY REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : NULL REMARK 245 SAMPLE SUPPORT DETAILS : NULL REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL REMARK 245 SAMPLE BUFFER : NULL REMARK 245 PH : 7.40 REMARK 245 SAMPLE DETAILS : NULL REMARK 245 REMARK 245 DATA ACQUISITION REMARK 245 DATE OF EXPERIMENT : NULL REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : NULL REMARK 245 TEMPERATURE (KELVIN) : NULL REMARK 245 MICROSCOPE MODEL : FEI TITAN KRIOS REMARK 245 DETECTOR TYPE : GATAN K3 BIOQUANTUM (6K X REMARK 245 4K) REMARK 245 MINIMUM DEFOCUS (NM) : 700.00 REMARK 245 MAXIMUM DEFOCUS (NM) : 2200.00 REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 NOMINAL CS : NULL REMARK 245 IMAGING MODE : BRIGHT FIELD REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 6000.00 REMARK 245 ILLUMINATION MODE : FLOOD BEAM REMARK 245 NOMINAL MAGNIFICATION : NULL REMARK 245 CALIBRATED MAGNIFICATION : NULL REMARK 245 SOURCE : FIELD EMISSION GUN REMARK 245 ACCELERATION VOLTAGE (KV) : 300 REMARK 245 IMAGING DETAILS : NULL REMARK 247 REMARK 247 ELECTRON MICROSCOPY REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION REMARK 247 OF THE STRUCTURE FACTORS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TETRAMERIC REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: TETRAMERIC REMARK 350 SOFTWARE USED: PISA REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 REMARK 465 M RES C SSSEQI REMARK 465 MET C -146 REMARK 465 LYS C -145 REMARK 465 THR C -144 REMARK 465 ILE C -143 REMARK 465 ILE C -142 REMARK 465 ALA C -141 REMARK 465 LEU C -140 REMARK 465 SER C -139 REMARK 465 TYR C -138 REMARK 465 ILE C -137 REMARK 465 PHE C -136 REMARK 465 CYS C -135 REMARK 465 LEU C -134 REMARK 465 VAL C -133 REMARK 465 PHE C -132 REMARK 465 ALA C -131 REMARK 465 ASP C -130 REMARK 465 TYR C -129 REMARK 465 LYS C -128 REMARK 465 ASP C -127 REMARK 465 ASP C -126 REMARK 465 ASP C -125 REMARK 465 ASP C -124 REMARK 465 LYS C -123 REMARK 465 HIS C -122 REMARK 465 HIS C -121 REMARK 465 HIS C -120 REMARK 465 HIS C -119 REMARK 465 HIS C -118 REMARK 465 HIS C -117 REMARK 465 HIS C -116 REMARK 465 HIS C -115 REMARK 465 HIS C -114 REMARK 465 HIS C -113 REMARK 465 LEU C -112 REMARK 465 GLU C -111 REMARK 465 VAL C -110 REMARK 465 LEU C -109 REMARK 465 PHE C -108 REMARK 465 GLN C -107 REMARK 465 GLY C -106 REMARK 465 PRO C -105 REMARK 465 ALA C -104 REMARK 465 ASP C -103 REMARK 465 LEU C -102 REMARK 465 GLU C -101 REMARK 465 ASP C -100 REMARK 465 ASN C -99 REMARK 465 TRP C -98 REMARK 465 GLU C -97 REMARK 465 THR C -96 REMARK 465 LEU C -95 REMARK 465 ASN C -94 REMARK 465 ASP C -93 REMARK 465 ASN C -92 REMARK 465 LEU C -91 REMARK 465 LYS C -90 REMARK 465 VAL C -89 REMARK 465 ILE C -88 REMARK 465 GLU C -87 REMARK 465 LYS C -86 REMARK 465 ALA C -85 REMARK 465 ASP C -84 REMARK 465 ASN C -83 REMARK 465 ALA C -82 REMARK 465 ALA C -81 REMARK 465 GLN C -80 REMARK 465 VAL C -79 REMARK 465 LYS C -78 REMARK 465 ASP C -77 REMARK 465 ALA C -76 REMARK 465 LEU C -75 REMARK 465 THR C -74 REMARK 465 LYS C -73 REMARK 465 MET C -72 REMARK 465 ARG C -71 REMARK 465 ALA C -70 REMARK 465 ALA C -69 REMARK 465 ALA C -68 REMARK 465 LEU C -67 REMARK 465 ASP C -66 REMARK 465 ALA C -65 REMARK 465 GLN C -64 REMARK 465 LYS C -63 REMARK 465 ALA C -62 REMARK 465 THR C -61 REMARK 465 PRO C -60 REMARK 465 PRO C -59 REMARK 465 LYS C -58 REMARK 465 LEU C -57 REMARK 465 GLU C -56 REMARK 465 ASP C -55 REMARK 465 LYS C -54 REMARK 465 SER C -53 REMARK 465 PRO C -52 REMARK 465 ASP C -51 REMARK 465 SER C -50 REMARK 465 PRO C -49 REMARK 465 GLU C -48 REMARK 465 MET C -47 REMARK 465 LYS C -46 REMARK 465 ASP C -45 REMARK 465 PHE C -44 REMARK 465 ARG C -43 REMARK 465 HIS C -42 REMARK 465 GLY C -41 REMARK 465 PHE C -40 REMARK 465 ASP C -39 REMARK 465 ILE C -38 REMARK 465 LEU C -37 REMARK 465 VAL C -36 REMARK 465 GLY C -35 REMARK 465 GLN C -34 REMARK 465 ILE C -33 REMARK 465 ASP C -32 REMARK 465 ASP C -31 REMARK 465 ALA C -30 REMARK 465 LEU C -29 REMARK 465 LYS C -28 REMARK 465 LEU C -27 REMARK 465 ALA C -26 REMARK 465 ASN C -25 REMARK 465 GLU C -24 REMARK 465 GLY C -23 REMARK 465 LYS C -22 REMARK 465 VAL C -21 REMARK 465 LYS C -20 REMARK 465 GLU C -19 REMARK 465 ALA C -18 REMARK 465 GLN C -17 REMARK 465 ALA C -16 REMARK 465 ALA C -15 REMARK 465 ALA C -14 REMARK 465 GLU C -13 REMARK 465 GLN C -12 REMARK 465 LEU C -11 REMARK 465 LYS C -10 REMARK 465 THR C -9 REMARK 465 THR C -8 REMARK 465 ARG C -7 REMARK 465 ASN C -6 REMARK 465 ALA C -5 REMARK 465 TYR C -4 REMARK 465 ILE C -3 REMARK 465 GLN C -2 REMARK 465 LYS C -1 REMARK 465 TYR C 0 REMARK 465 LEU C 1 REMARK 465 GLU C 2 REMARK 465 GLU C 3 REMARK 465 GLY C 4 REMARK 465 GLY C 5 REMARK 465 ASP C 6 REMARK 465 PHE C 7 REMARK 465 ASP C 8 REMARK 465 ASN C 9 REMARK 465 TYR C 10 REMARK 465 TYR C 11 REMARK 465 GLY C 12 REMARK 465 ALA C 13 REMARK 465 ASP C 14 REMARK 465 ASN C 15 REMARK 465 GLN C 16 REMARK 465 SER C 17 REMARK 465 GLU C 18 REMARK 465 CYS C 19 REMARK 465 GLU C 20 REMARK 465 TYR C 21 REMARK 465 THR C 22 REMARK 465 ASP C 23 REMARK 465 TRP C 24 REMARK 465 CYS C 320 REMARK 465 THR C 321 REMARK 465 SER C 322 REMARK 465 MET C 323 REMARK 465 LEU C 324 REMARK 465 CYS C 325 REMARK 465 CYS C 326 REMARK 465 GLY C 327 REMARK 465 GLN C 328 REMARK 465 SER C 329 REMARK 465 ARG C 330 REMARK 465 MET D -146 REMARK 465 LYS D -145 REMARK 465 THR D -144 REMARK 465 ILE D -143 REMARK 465 ILE D -142 REMARK 465 ALA D -141 REMARK 465 LEU D -140 REMARK 465 SER D -139 REMARK 465 TYR D -138 REMARK 465 ILE D -137 REMARK 465 PHE D -136 REMARK 465 CYS D -135 REMARK 465 LEU D -134 REMARK 465 VAL D -133 REMARK 465 PHE D -132 REMARK 465 ALA D -131 REMARK 465 ASP D -130 REMARK 465 TYR D -129 REMARK 465 LYS D -128 REMARK 465 ASP D -127 REMARK 465 ASP D -126 REMARK 465 ASP D -125 REMARK 465 ASP D -124 REMARK 465 LYS D -123 REMARK 465 HIS D -122 REMARK 465 HIS D -121 REMARK 465 HIS D -120 REMARK 465 HIS D -119 REMARK 465 HIS D -118 REMARK 465 HIS D -117 REMARK 465 HIS D -116 REMARK 465 HIS D -115 REMARK 465 HIS D -114 REMARK 465 HIS D -113 REMARK 465 LEU D -112 REMARK 465 GLU D -111 REMARK 465 VAL D -110 REMARK 465 LEU D -109 REMARK 465 PHE D -108 REMARK 465 GLN D -107 REMARK 465 GLY D -106 REMARK 465 PRO D -105 REMARK 465 ALA D -104 REMARK 465 ASP D -103 REMARK 465 LEU D -102 REMARK 465 GLU D -101 REMARK 465 ASP D -100 REMARK 465 ASN D -99 REMARK 465 TRP D -98 REMARK 465 GLU D -97 REMARK 465 THR D -96 REMARK 465 LEU D -95 REMARK 465 ASN D -94 REMARK 465 ASP D -93 REMARK 465 ASN D -92 REMARK 465 LEU D -91 REMARK 465 LYS D -90 REMARK 465 VAL D -89 REMARK 465 ILE D -88 REMARK 465 GLU D -87 REMARK 465 LYS D -86 REMARK 465 ALA D -85 REMARK 465 ASP D -84 REMARK 465 ASN D -83 REMARK 465 ALA D -82 REMARK 465 ALA D -81 REMARK 465 GLN D -80 REMARK 465 VAL D -79 REMARK 465 LYS D -78 REMARK 465 ASP D -77 REMARK 465 ALA D -76 REMARK 465 LEU D -75 REMARK 465 THR D -74 REMARK 465 LYS D -73 REMARK 465 MET D -72 REMARK 465 ARG D -71 REMARK 465 ALA D -70 REMARK 465 ALA D -69 REMARK 465 ALA D -68 REMARK 465 LEU D -67 REMARK 465 ASP D -66 REMARK 465 ALA D -65 REMARK 465 GLN D -64 REMARK 465 LYS D -63 REMARK 465 ALA D -62 REMARK 465 THR D -61 REMARK 465 PRO D -60 REMARK 465 PRO D -59 REMARK 465 LYS D -58 REMARK 465 LEU D -57 REMARK 465 GLU D -56 REMARK 465 ASP D -55 REMARK 465 LYS D -54 REMARK 465 SER D -53 REMARK 465 PRO D -52 REMARK 465 ASP D -51 REMARK 465 SER D -50 REMARK 465 PRO D -49 REMARK 465 GLU D -48 REMARK 465 MET D -47 REMARK 465 LYS D -46 REMARK 465 ASP D -45 REMARK 465 PHE D -44 REMARK 465 ARG D -43 REMARK 465 HIS D -42 REMARK 465 GLY D -41 REMARK 465 PHE D -40 REMARK 465 ASP D -39 REMARK 465 ILE D -38 REMARK 465 LEU D -37 REMARK 465 VAL D -36 REMARK 465 GLY D -35 REMARK 465 GLN D -34 REMARK 465 ILE D -33 REMARK 465 ASP D -32 REMARK 465 ASP D -31 REMARK 465 ALA D -30 REMARK 465 LEU D -29 REMARK 465 LYS D -28 REMARK 465 LEU D -27 REMARK 465 ALA D -26 REMARK 465 ASN D -25 REMARK 465 GLU D -24 REMARK 465 GLY D -23 REMARK 465 LYS D -22 REMARK 465 VAL D -21 REMARK 465 LYS D -20 REMARK 465 GLU D -19 REMARK 465 ALA D -18 REMARK 465 GLN D -17 REMARK 465 ALA D -16 REMARK 465 ALA D -15 REMARK 465 ALA D -14 REMARK 465 GLU D -13 REMARK 465 GLN D -12 REMARK 465 LEU D -11 REMARK 465 LYS D -10 REMARK 465 THR D -9 REMARK 465 THR D -8 REMARK 465 ARG D -7 REMARK 465 ASN D -6 REMARK 465 ALA D -5 REMARK 465 TYR D -4 REMARK 465 ILE D -3 REMARK 465 GLN D -2 REMARK 465 LYS D -1 REMARK 465 TYR D 0 REMARK 465 LEU D 1 REMARK 465 GLU D 2 REMARK 465 GLU D 3 REMARK 465 GLY D 4 REMARK 465 GLY D 5 REMARK 465 ASP D 6 REMARK 465 PHE D 7 REMARK 465 ASP D 8 REMARK 465 ASN D 9 REMARK 465 TYR D 10 REMARK 465 TYR D 11 REMARK 465 GLY D 12 REMARK 465 ALA D 13 REMARK 465 ASP D 14 REMARK 465 ASN D 15 REMARK 465 GLN D 16 REMARK 465 SER D 17 REMARK 465 GLU D 18 REMARK 465 CYS D 19 REMARK 465 GLU D 20 REMARK 465 TYR D 21 REMARK 465 THR D 22 REMARK 465 ASP D 23 REMARK 465 TRP D 24 REMARK 465 LYS D 25 REMARK 465 SER D 26 REMARK 465 SER D 27 REMARK 465 CYS D 320 REMARK 465 THR D 321 REMARK 465 SER D 322 REMARK 465 MET D 323 REMARK 465 LEU D 324 REMARK 465 CYS D 325 REMARK 465 CYS D 326 REMARK 465 GLY D 327 REMARK 465 GLN D 328 REMARK 465 SER D 329 REMARK 465 ARG D 330 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: COVALENT BOND ANGLES REMARK 500 REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1) REMARK 500 REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999 REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996 REMARK 500 REMARK 500 M RES CSSEQI ATM1 ATM2 ATM3 REMARK 500 LEU D 218 CA - CB - CG ANGL. DEV. = 19.5 DEGREES REMARK 500 REMARK 500 REMARK: NULL REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 ALA A 49 116.19 -163.53 REMARK 500 VAL B 48 -54.90 -124.02 REMARK 500 MET B 114 31.10 -98.86 REMARK 500 SER B 117 58.26 -96.50 REMARK 500 ARG C 91 30.01 -91.98 REMARK 500 TYR C 93 60.20 60.51 REMARK 500 PRO C 96 50.58 -90.11 REMARK 500 PHE C 210 -10.17 -140.89 REMARK 500 GLU C 235 -168.28 71.93 REMARK 500 PRO D 96 49.27 -90.14 REMARK 500 ILE D 131 -60.59 -99.98 REMARK 500 ARG D 236 58.49 35.82 REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: EMD-39810 RELATED DB: EMDB REMARK 900 CRYO-EM STRUCTURE OF APJR COMPLEX WITH AGONISTIC ANTIBODY DBREF 8Z74 A 1 130 PDB 8Z74 8Z74 1 130 DBREF 8Z74 B 1 130 PDB 8Z74 8Z74 1 130 DBREF 8Z74 C -104 0 UNP P0ABE7 C562_ECOLX 23 127 DBREF 8Z74 C 2 330 UNP P35414 APJ_HUMAN 2 330 DBREF 8Z74 D -104 0 UNP P0ABE7 C562_ECOLX 23 127 DBREF 8Z74 D 2 330 UNP P35414 APJ_HUMAN 2 330 SEQADV 8Z74 MET C -146 UNP P0ABE7 INITIATING METHIONINE SEQADV 8Z74 LYS C -145 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 THR C -144 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ILE C -143 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ILE C -142 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ALA C -141 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LEU C -140 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 SER C -139 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 TYR C -138 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ILE C -137 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 PHE C -136 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 CYS C -135 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LEU C -134 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 VAL C -133 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 PHE C -132 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ALA C -131 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP C -130 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 TYR C -129 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LYS C -128 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP C -127 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP C -126 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP C -125 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP C -124 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LYS C -123 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -122 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -121 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -120 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -119 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -118 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -117 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -116 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -115 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -114 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS C -113 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LEU C -112 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 GLU C -111 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 VAL C -110 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LEU C -109 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 PHE C -108 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 GLN C -107 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 GLY C -106 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 PRO C -105 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 TRP C -98 UNP P0ABE7 MET 29 CONFLICT SEQADV 8Z74 ILE C -3 UNP P0ABE7 HIS 124 CONFLICT SEQADV 8Z74 LEU C 1 UNP P0ABE7 LINKER SEQADV 8Z74 MET D -146 UNP P0ABE7 INITIATING METHIONINE SEQADV 8Z74 LYS D -145 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 THR D -144 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ILE D -143 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ILE D -142 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ALA D -141 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LEU D -140 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 SER D -139 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 TYR D -138 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ILE D -137 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 PHE D -136 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 CYS D -135 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LEU D -134 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 VAL D -133 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 PHE D -132 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ALA D -131 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP D -130 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 TYR D -129 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LYS D -128 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP D -127 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP D -126 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP D -125 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 ASP D -124 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LYS D -123 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -122 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -121 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -120 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -119 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -118 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -117 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -116 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -115 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -114 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 HIS D -113 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LEU D -112 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 GLU D -111 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 VAL D -110 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 LEU D -109 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 PHE D -108 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 GLN D -107 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 GLY D -106 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 PRO D -105 UNP P0ABE7 EXPRESSION TAG SEQADV 8Z74 TRP D -98 UNP P0ABE7 MET 29 CONFLICT SEQADV 8Z74 ILE D -3 UNP P0ABE7 HIS 124 CONFLICT SEQADV 8Z74 LEU D 1 UNP P0ABE7 LINKER SEQRES 1 A 130 GLN VAL GLN LEU VAL GLU SER GLY GLY GLY SER VAL GLN SEQRES 2 A 130 SER GLY GLY SER LEU THR LEU SER CYS ALA ALA SER GLY SEQRES 3 A 130 SER THR TYR SER SER HIS CYS MET GLY TRP PHE ARG GLN SEQRES 4 A 130 ALA PRO GLY LYS GLU ARG GLU GLY VAL ALA LEU MET THR SEQRES 5 A 130 ARG SER ARG GLY THR SER TYR ALA ASP SER VAL LYS GLY SEQRES 6 A 130 ARG PHE THR ILE SER GLN ASP ASN THR LYS ASN ILE LEU SEQRES 7 A 130 TYR LEU GLN MET ASN SER LEU LYS PRO GLU ASP THR ALA SEQRES 8 A 130 MET TYR TYR CYS ALA ALA VAL PRO ARG ALA GLY ILE GLU SEQRES 9 A 130 TYR SER GLY ALA TYR CYS LYS TRP ASN MET LYS ASP SER SEQRES 10 A 130 GLY SER TRP GLY GLN GLY THR GLN VAL THR VAL SER SER SEQRES 1 B 130 GLN VAL GLN LEU VAL GLU SER GLY GLY GLY SER VAL GLN SEQRES 2 B 130 SER GLY GLY SER LEU THR LEU SER CYS ALA ALA SER GLY SEQRES 3 B 130 SER THR TYR SER SER HIS CYS MET GLY TRP PHE ARG GLN SEQRES 4 B 130 ALA PRO GLY LYS GLU ARG GLU GLY VAL ALA LEU MET THR SEQRES 5 B 130 ARG SER ARG GLY THR SER TYR ALA ASP SER VAL LYS GLY SEQRES 6 B 130 ARG PHE THR ILE SER GLN ASP ASN THR LYS ASN ILE LEU SEQRES 7 B 130 TYR LEU GLN MET ASN SER LEU LYS PRO GLU ASP THR ALA SEQRES 8 B 130 MET TYR TYR CYS ALA ALA VAL PRO ARG ALA GLY ILE GLU SEQRES 9 B 130 TYR SER GLY ALA TYR CYS LYS TRP ASN MET LYS ASP SER SEQRES 10 B 130 GLY SER TRP GLY GLN GLY THR GLN VAL THR VAL SER SER SEQRES 1 C 477 MET LYS THR ILE ILE ALA LEU SER TYR ILE PHE CYS LEU SEQRES 2 C 477 VAL PHE ALA ASP TYR LYS ASP ASP ASP ASP LYS HIS HIS SEQRES 3 C 477 HIS HIS HIS HIS HIS HIS HIS HIS LEU GLU VAL LEU PHE SEQRES 4 C 477 GLN GLY PRO ALA ASP LEU GLU ASP ASN TRP GLU THR LEU SEQRES 5 C 477 ASN ASP ASN LEU LYS VAL ILE GLU LYS ALA ASP ASN ALA SEQRES 6 C 477 ALA GLN VAL LYS ASP ALA LEU THR LYS MET ARG ALA ALA SEQRES 7 C 477 ALA LEU ASP ALA GLN LYS ALA THR PRO PRO LYS LEU GLU SEQRES 8 C 477 ASP LYS SER PRO ASP SER PRO GLU MET LYS ASP PHE ARG SEQRES 9 C 477 HIS GLY PHE ASP ILE LEU VAL GLY GLN ILE ASP ASP ALA SEQRES 10 C 477 LEU LYS LEU ALA ASN GLU GLY LYS VAL LYS GLU ALA GLN SEQRES 11 C 477 ALA ALA ALA GLU GLN LEU LYS THR THR ARG ASN ALA TYR SEQRES 12 C 477 ILE GLN LYS TYR LEU GLU GLU GLY GLY ASP PHE ASP ASN SEQRES 13 C 477 TYR TYR GLY ALA ASP ASN GLN SER GLU CYS GLU TYR THR SEQRES 14 C 477 ASP TRP LYS SER SER GLY ALA LEU ILE PRO ALA ILE TYR SEQRES 15 C 477 MET LEU VAL PHE LEU LEU GLY THR THR GLY ASN GLY LEU SEQRES 16 C 477 VAL LEU TRP THR VAL PHE ARG SER SER ARG GLU LYS ARG SEQRES 17 C 477 ARG SER ALA ASP ILE PHE ILE ALA SER LEU ALA VAL ALA SEQRES 18 C 477 ASP LEU THR PHE VAL VAL THR LEU PRO LEU TRP ALA THR SEQRES 19 C 477 TYR THR TYR ARG ASP TYR ASP TRP PRO PHE GLY THR PHE SEQRES 20 C 477 PHE CYS LYS LEU SER SER TYR LEU ILE PHE VAL ASN MET SEQRES 21 C 477 TYR ALA SER VAL PHE CYS LEU THR GLY LEU SER PHE ASP SEQRES 22 C 477 ARG TYR LEU ALA ILE VAL ARG PRO VAL ALA ASN ALA ARG SEQRES 23 C 477 LEU ARG LEU ARG VAL SER GLY ALA VAL ALA THR ALA VAL SEQRES 24 C 477 LEU TRP VAL LEU ALA ALA LEU LEU ALA MET PRO VAL MET SEQRES 25 C 477 VAL LEU ARG THR THR GLY ASP LEU GLU ASN THR THR LYS SEQRES 26 C 477 VAL GLN CYS TYR MET ASP TYR SER MET VAL ALA THR VAL SEQRES 27 C 477 SER SER GLU TRP ALA TRP GLU VAL GLY LEU GLY VAL SER SEQRES 28 C 477 SER THR THR VAL GLY PHE VAL VAL PRO PHE THR ILE MET SEQRES 29 C 477 LEU THR CYS TYR PHE PHE ILE ALA GLN THR ILE ALA GLY SEQRES 30 C 477 HIS PHE ARG LYS GLU ARG ILE GLU GLY LEU ARG LYS ARG SEQRES 31 C 477 ARG ARG LEU LEU SER ILE ILE VAL VAL LEU VAL VAL THR SEQRES 32 C 477 PHE ALA LEU CYS TRP MET PRO TYR HIS LEU VAL LYS THR SEQRES 33 C 477 LEU TYR MET LEU GLY SER LEU LEU HIS TRP PRO CYS ASP SEQRES 34 C 477 PHE ASP LEU PHE LEU MET ASN ILE PHE PRO TYR CYS THR SEQRES 35 C 477 CYS ILE SER TYR VAL ASN SER CYS LEU ASN PRO PHE LEU SEQRES 36 C 477 TYR ALA PHE PHE ASP PRO ARG PHE ARG GLN ALA CYS THR SEQRES 37 C 477 SER MET LEU CYS CYS GLY GLN SER ARG SEQRES 1 D 477 MET LYS THR ILE ILE ALA LEU SER TYR ILE PHE CYS LEU SEQRES 2 D 477 VAL PHE ALA ASP TYR LYS ASP ASP ASP ASP LYS HIS HIS SEQRES 3 D 477 HIS HIS HIS HIS HIS HIS HIS HIS LEU GLU VAL LEU PHE SEQRES 4 D 477 GLN GLY PRO ALA ASP LEU GLU ASP ASN TRP GLU THR LEU SEQRES 5 D 477 ASN ASP ASN LEU LYS VAL ILE GLU LYS ALA ASP ASN ALA SEQRES 6 D 477 ALA GLN VAL LYS ASP ALA LEU THR LYS MET ARG ALA ALA SEQRES 7 D 477 ALA LEU ASP ALA GLN LYS ALA THR PRO PRO LYS LEU GLU SEQRES 8 D 477 ASP LYS SER PRO ASP SER PRO GLU MET LYS ASP PHE ARG SEQRES 9 D 477 HIS GLY PHE ASP ILE LEU VAL GLY GLN ILE ASP ASP ALA SEQRES 10 D 477 LEU LYS LEU ALA ASN GLU GLY LYS VAL LYS GLU ALA GLN SEQRES 11 D 477 ALA ALA ALA GLU GLN LEU LYS THR THR ARG ASN ALA TYR SEQRES 12 D 477 ILE GLN LYS TYR LEU GLU GLU GLY GLY ASP PHE ASP ASN SEQRES 13 D 477 TYR TYR GLY ALA ASP ASN GLN SER GLU CYS GLU TYR THR SEQRES 14 D 477 ASP TRP LYS SER SER GLY ALA LEU ILE PRO ALA ILE TYR SEQRES 15 D 477 MET LEU VAL PHE LEU LEU GLY THR THR GLY ASN GLY LEU SEQRES 16 D 477 VAL LEU TRP THR VAL PHE ARG SER SER ARG GLU LYS ARG SEQRES 17 D 477 ARG SER ALA ASP ILE PHE ILE ALA SER LEU ALA VAL ALA SEQRES 18 D 477 ASP LEU THR PHE VAL VAL THR LEU PRO LEU TRP ALA THR SEQRES 19 D 477 TYR THR TYR ARG ASP TYR ASP TRP PRO PHE GLY THR PHE SEQRES 20 D 477 PHE CYS LYS LEU SER SER TYR LEU ILE PHE VAL ASN MET SEQRES 21 D 477 TYR ALA SER VAL PHE CYS LEU THR GLY LEU SER PHE ASP SEQRES 22 D 477 ARG TYR LEU ALA ILE VAL ARG PRO VAL ALA ASN ALA ARG SEQRES 23 D 477 LEU ARG LEU ARG VAL SER GLY ALA VAL ALA THR ALA VAL SEQRES 24 D 477 LEU TRP VAL LEU ALA ALA LEU LEU ALA MET PRO VAL MET SEQRES 25 D 477 VAL LEU ARG THR THR GLY ASP LEU GLU ASN THR THR LYS SEQRES 26 D 477 VAL GLN CYS TYR MET ASP TYR SER MET VAL ALA THR VAL SEQRES 27 D 477 SER SER GLU TRP ALA TRP GLU VAL GLY LEU GLY VAL SER SEQRES 28 D 477 SER THR THR VAL GLY PHE VAL VAL PRO PHE THR ILE MET SEQRES 29 D 477 LEU THR CYS TYR PHE PHE ILE ALA GLN THR ILE ALA GLY SEQRES 30 D 477 HIS PHE ARG LYS GLU ARG ILE GLU GLY LEU ARG LYS ARG SEQRES 31 D 477 ARG ARG LEU LEU SER ILE ILE VAL VAL LEU VAL VAL THR SEQRES 32 D 477 PHE ALA LEU CYS TRP MET PRO TYR HIS LEU VAL LYS THR SEQRES 33 D 477 LEU TYR MET LEU GLY SER LEU LEU HIS TRP PRO CYS ASP SEQRES 34 D 477 PHE ASP LEU PHE LEU MET ASN ILE PHE PRO TYR CYS THR SEQRES 35 D 477 CYS ILE SER TYR VAL ASN SER CYS LEU ASN PRO PHE LEU SEQRES 36 D 477 TYR ALA PHE PHE ASP PRO ARG PHE ARG GLN ALA CYS THR SEQRES 37 D 477 SER MET LEU CYS CYS GLY GLN SER ARG HET CLR D 401 28 HET CLR D 402 28 HETNAM CLR CHOLESTEROL FORMUL 5 CLR 2(C27 H46 O) HELIX 1 AA1 ASP A 61 LYS A 64 5 4 HELIX 2 AA2 LYS A 86 THR A 90 5 5 HELIX 3 AA3 LYS A 111 LYS A 115 5 5 HELIX 4 AA4 LYS B 86 THR B 90 5 5 HELIX 5 AA5 LYS B 111 LYS B 115 5 5 HELIX 6 AA6 ALA C 29 SER C 56 1 28 HELIX 7 AA7 ARG C 62 ARG C 91 1 30 HELIX 8 AA8 GLY C 98 ARG C 133 1 36 HELIX 9 AA9 ARG C 133 VAL C 144 1 12 HELIX 10 AB1 SER C 145 LEU C 167 1 23 HELIX 11 AB2 ASP C 172 THR C 176 5 5 HELIX 12 AB3 THR C 190 VAL C 211 1 22 HELIX 13 AB4 VAL C 211 ARG C 233 1 23 HELIX 14 AB5 ARG C 236 TRP C 261 1 26 HELIX 15 AB6 TRP C 261 HIS C 278 1 18 HELIX 16 AB7 PRO C 280 ASP C 313 1 34 HELIX 17 AB8 ASP C 313 ALA C 319 1 7 HELIX 18 AB9 ALA D 29 SER D 56 1 28 HELIX 19 AC1 ALA D 64 VAL D 80 1 17 HELIX 20 AC2 THR D 81 ARG D 91 1 11 HELIX 21 AC3 GLY D 98 ARG D 133 1 36 HELIX 22 AC4 ARG D 133 VAL D 144 1 12 HELIX 23 AC5 SER D 145 LEU D 167 1 23 HELIX 24 AC6 ASP D 172 THR D 176 5 5 HELIX 25 AC7 THR D 190 PHE D 210 1 21 HELIX 26 AC8 PHE D 210 ARG D 233 1 24 HELIX 27 AC9 ILE D 237 LEU D 246 1 10 HELIX 28 AD1 LEU D 246 HIS D 278 1 33 HELIX 29 AD2 PRO D 280 ASP D 313 1 34 HELIX 30 AD3 ASP D 313 ALA D 319 1 7 SHEET 1 AA1 2 SER A 11 GLN A 13 0 SHEET 2 AA1 2 THR A 127 SER A 129 1 O SER A 129 N VAL A 12 SHEET 1 AA2 3 LEU A 18 ALA A 23 0 SHEET 2 AA2 3 ILE A 77 MET A 82 -1 O LEU A 80 N LEU A 20 SHEET 3 AA2 3 ILE A 69 ASP A 72 -1 N SER A 70 O TYR A 79 SHEET 1 AA3 5 SER A 58 TYR A 59 0 SHEET 2 AA3 5 GLU A 46 LEU A 50 -1 N LEU A 50 O SER A 58 SHEET 3 AA3 5 HIS A 32 ARG A 38 -1 N TRP A 36 O ALA A 49 SHEET 4 AA3 5 MET A 92 PRO A 99 -1 O TYR A 94 N PHE A 37 SHEET 5 AA3 5 THR A 124 GLN A 125 -1 O THR A 124 N TYR A 93 SHEET 1 AA4 4 GLU B 6 SER B 7 0 SHEET 2 AA4 4 GLY B 16 ALA B 23 -1 O SER B 21 N SER B 7 SHEET 3 AA4 4 ILE B 77 LEU B 85 -1 O LEU B 78 N CYS B 22 SHEET 4 AA4 4 PHE B 67 ASP B 72 -1 N THR B 68 O GLN B 81 SHEET 1 AA5 3 ARG B 45 ALA B 49 0 SHEET 2 AA5 3 HIS B 32 GLN B 39 -1 N TRP B 36 O ALA B 49 SHEET 3 AA5 3 MET B 92 PRO B 99 -1 O TYR B 94 N PHE B 37 SHEET 1 AA6 2 ARG C 168 GLY C 171 0 SHEET 2 AA6 2 GLN C 180 MET C 183 -1 O TYR C 182 N THR C 169 SHEET 1 AA7 2 ARG D 168 GLY D 171 0 SHEET 2 AA7 2 GLN D 180 MET D 183 -1 O TYR D 182 N THR D 169 SSBOND 1 CYS A 22 CYS A 95 1555 1555 2.03 SSBOND 2 CYS A 33 CYS A 110 1555 1555 2.02 SSBOND 3 CYS B 22 CYS B 95 1555 1555 2.03 SSBOND 4 CYS B 33 CYS B 110 1555 1555 2.03 SSBOND 5 CYS C 102 CYS C 181 1555 1555 2.03 SSBOND 6 CYS D 102 CYS D 181 1555 1555 2.03 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000