HEADER MEMBRANE PROTEIN 23-MAY-24 9FFZ TITLE CRYO-EM STRUCTURE OF THE ALPHA1BETA3GAMMA2 GABA(A) RECEPTOR IN COMPLEX TITLE 2 WITH GABA AND NB38 IN THE SHORT-LIVED ASYMMETRIC DESENSITISED 1 STATE CAVEAT 9FFZ MAN A 5 HAS WRONG CHIRALITY AT ATOM C1 MAN A 6 HAS WRONG CAVEAT 2 9FFZ CHIRALITY AT ATOM C1 D3D A 502 HAS WRONG CHIRALITY AT ATOM CAVEAT 3 9FFZ C17 D3D A 502 HAS WRONG CHIRALITY AT ATOM C20 COMPND MOL_ID: 1; COMPND 2 MOLECULE: GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT ALPHA-1; COMPND 3 CHAIN: A, D; COMPND 4 SYNONYM: GABA(A) RECEPTOR SUBUNIT ALPHA-1,GABAAR SUBUNIT ALPHA-1; COMPND 5 ENGINEERED: YES; COMPND 6 MOL_ID: 2; COMPND 7 MOLECULE: GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT BETA-3; COMPND 8 CHAIN: B, E; COMPND 9 SYNONYM: GABA(A) RECEPTOR SUBUNIT BETA-3,GABAAR SUBUNIT BETA-3; COMPND 10 ENGINEERED: YES; COMPND 11 MOL_ID: 3; COMPND 12 MOLECULE: ISOFORM 1 OF GAMMA-AMINOBUTYRIC ACID RECEPTOR SUBUNIT COMPND 13 GAMMA-2; COMPND 14 CHAIN: C; COMPND 15 SYNONYM: GABA(A) RECEPTOR SUBUNIT GAMMA-2,GABAAR SUBUNIT GAMMA-2; COMPND 16 ENGINEERED: YES; COMPND 17 MOL_ID: 4; COMPND 18 MOLECULE: NANOBODY38; COMPND 19 CHAIN: F; COMPND 20 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_COMMON: HUMAN; SOURCE 4 ORGANISM_TAXID: 9606; SOURCE 5 GENE: GABRA1; SOURCE 6 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 7 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 8 EXPRESSION_SYSTEM_CELL_LINE: HEK293S GNTI-; SOURCE 9 EXPRESSION_SYSTEM_PLASMID: PHR-TETO2; SOURCE 10 MOL_ID: 2; SOURCE 11 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 12 ORGANISM_COMMON: HUMAN; SOURCE 13 ORGANISM_TAXID: 9606; SOURCE 14 GENE: GABRB3; SOURCE 15 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 16 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 17 EXPRESSION_SYSTEM_CELL_LINE: HEK293S GNTI-; SOURCE 18 EXPRESSION_SYSTEM_PLASMID: PHR-TETO2; SOURCE 19 MOL_ID: 3; SOURCE 20 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 21 ORGANISM_COMMON: HUMAN; SOURCE 22 ORGANISM_TAXID: 9606; SOURCE 23 GENE: GABRG2; SOURCE 24 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 25 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 26 EXPRESSION_SYSTEM_CELL_LINE: HEK293S GNTI-; SOURCE 27 EXPRESSION_SYSTEM_PLASMID: PHR-TETO2; SOURCE 28 MOL_ID: 4; SOURCE 29 ORGANISM_SCIENTIFIC: LAMA GLAMA; SOURCE 30 ORGANISM_TAXID: 9844; SOURCE 31 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 32 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 33 EXPRESSION_SYSTEM_STRAIN: WK6 SU- KEYWDS GABA, NEUROTRANSMISSION, GATING CYCLE, TIME-RESOLVED CRYO-EM, KEYWDS 2 MEMBRANE PROTEIN EXPDTA ELECTRON MICROSCOPY AUTHOR D.B.MIHAYLOV,T.MALINAUSKAS,A.R.ARICESCU REVDAT 1 04-JUN-25 9FFZ 0 JRNL AUTH D.B.MIHAYLOV,T.MALINAUSKAS,A.R.ARICESCU JRNL TITL CRYO-EM STRUCTURE OF THE ALPHA1BETA3GAMMA2 GABA(A) RECEPTOR JRNL TITL 2 IN COMPLEX WITH GABA AND NB38 IN THE SHORT-LIVED ASYMMETRIC JRNL TITL 3 DESENSITISED 1 STATE JRNL REF TO BE PUBLISHED JRNL REFN REMARK 2 REMARK 2 RESOLUTION. 3.30 ANGSTROMS. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 SOFTWARE PACKAGES : TOPAZ, EPU, CTFFIND, PHENIX, PHENIX, REMARK 3 CRYOSPARC, CRYOSPARC, CRYOSPARC, REMARK 3 CRYOSPARC REMARK 3 RECONSTRUCTION SCHEMA : NULL REMARK 3 REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT REMARK 3 PDB ENTRY : 6HUK REMARK 3 REFINEMENT SPACE : REAL REMARK 3 REFINEMENT PROTOCOL : OTHER REMARK 3 REFINEMENT TARGET : FSC AT 0.5 REMARK 3 OVERALL ANISOTROPIC B VALUE : 30.000 REMARK 3 REMARK 3 FITTING PROCEDURE : NULL REMARK 3 REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 3.300 REMARK 3 NUMBER OF PARTICLES : 53000 REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING AND AMPLITUDE REMARK 3 CORRECTION REMARK 3 REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL REMARK 3 REMARK 3 OTHER DETAILS: NULL REMARK 4 REMARK 4 9FFZ COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 07-JUN-24. REMARK 100 THE DEPOSITION ID IS D_1292138690. REMARK 245 REMARK 245 EXPERIMENTAL DETAILS REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE REMARK 245 SPECIMEN TYPE : NULL REMARK 245 REMARK 245 ELECTRON MICROSCOPE SAMPLE REMARK 245 SAMPLE TYPE : PARTICLE REMARK 245 PARTICLE TYPE : POINT REMARK 245 NAME OF SAMPLE : CRYO-EM STRUCTURE OF THE REMARK 245 ALPHA1BETA3GAMMA2 GABA(A) REMARK 245 RECEPTOR IN COMPLEX WITH GABA REMARK 245 AND NB38 IN THE SHORT-LIVED REMARK 245 ASYMMETRIC DESENSITISED 1 STATE REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : 1.00 REMARK 245 SAMPLE SUPPORT DETAILS : CURRENT: 30MA REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL REMARK 245 SAMPLE BUFFER : NULL REMARK 245 PH : 7.40 REMARK 245 SAMPLE DETAILS : NULL REMARK 245 REMARK 245 DATA ACQUISITION REMARK 245 DATE OF EXPERIMENT : NULL REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : 20929 REMARK 245 TEMPERATURE (KELVIN) : NULL REMARK 245 MICROSCOPE MODEL : TFS KRIOS REMARK 245 DETECTOR TYPE : TFS FALCON 4I (4K X 4K) REMARK 245 MINIMUM DEFOCUS (NM) : 1400.00 REMARK 245 MAXIMUM DEFOCUS (NM) : 2400.00 REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 NOMINAL CS : 2.70 REMARK 245 IMAGING MODE : BRIGHT FIELD REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 4000.00 REMARK 245 ILLUMINATION MODE : FLOOD BEAM REMARK 245 NOMINAL MAGNIFICATION : 96000 REMARK 245 CALIBRATED MAGNIFICATION : 96000 REMARK 245 SOURCE : FIELD EMISSION GUN REMARK 245 ACCELERATION VOLTAGE (KV) : 300 REMARK 245 IMAGING DETAILS : NULL REMARK 247 REMARK 247 ELECTRON MICROSCOPY REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION REMARK 247 OF THE STRUCTURE FACTORS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: HEXAMERIC REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: HEXAMERIC REMARK 350 SOFTWARE USED: PISA REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, F, a, b, G, c, REMARK 350 AND CHAINS: d, e, H REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 REMARK 465 M RES C SSSEQI REMARK 465 MET A -52 REMARK 465 ASP A -51 REMARK 465 GLU A -50 REMARK 465 LYS A -49 REMARK 465 THR A -48 REMARK 465 THR A -47 REMARK 465 GLY A -46 REMARK 465 TRP A -45 REMARK 465 ARG A -44 REMARK 465 GLY A -43 REMARK 465 GLY A -42 REMARK 465 HIS A -41 REMARK 465 VAL A -40 REMARK 465 VAL A -39 REMARK 465 GLU A -38 REMARK 465 GLY A -37 REMARK 465 LEU A -36 REMARK 465 ALA A -35 REMARK 465 GLY A -34 REMARK 465 GLU A -33 REMARK 465 LEU A -32 REMARK 465 GLU A -31 REMARK 465 GLN A -30 REMARK 465 LEU A -29 REMARK 465 ARG A -28 REMARK 465 ALA A -27 REMARK 465 ARG A -26 REMARK 465 LEU A -25 REMARK 465 GLU A -24 REMARK 465 HIS A -23 REMARK 465 HIS A -22 REMARK 465 PRO A -21 REMARK 465 GLN A -20 REMARK 465 GLY A -19 REMARK 465 GLN A -18 REMARK 465 ARG A -17 REMARK 465 GLU A -16 REMARK 465 PRO A -15 REMARK 465 ASP A -14 REMARK 465 TYR A -13 REMARK 465 ASP A -12 REMARK 465 ILE A -11 REMARK 465 PRO A -10 REMARK 465 THR A -9 REMARK 465 THR A -8 REMARK 465 GLU A -7 REMARK 465 ASN A -6 REMARK 465 LEU A -5 REMARK 465 TYR A -4 REMARK 465 PHE A -3 REMARK 465 GLN A -2 REMARK 465 GLY A -1 REMARK 465 THR A 0 REMARK 465 GLY A 1 REMARK 465 GLN A 2 REMARK 465 PRO A 3 REMARK 465 SER A 4 REMARK 465 GLN A 5 REMARK 465 ASP A 6 REMARK 465 GLU A 7 REMARK 465 LEU A 8 REMARK 465 LYS A 9 REMARK 465 GLU A 419 REMARK 465 PRO A 420 REMARK 465 GLN A 421 REMARK 465 LEU A 422 REMARK 465 LYS A 423 REMARK 465 ALA A 424 REMARK 465 PRO A 425 REMARK 465 THR A 426 REMARK 465 PRO A 427 REMARK 465 HIS A 428 REMARK 465 GLN A 429 REMARK 465 MET B -53 REMARK 465 ASP B -52 REMARK 465 GLU B -51 REMARK 465 LYS B -50 REMARK 465 THR B -49 REMARK 465 THR B -48 REMARK 465 GLY B -47 REMARK 465 TRP B -46 REMARK 465 ARG B -45 REMARK 465 GLY B -44 REMARK 465 GLY B -43 REMARK 465 HIS B -42 REMARK 465 VAL B -41 REMARK 465 VAL B -40 REMARK 465 GLU B -39 REMARK 465 GLY B -38 REMARK 465 LEU B -37 REMARK 465 ALA B -36 REMARK 465 GLY B -35 REMARK 465 GLU B -34 REMARK 465 LEU B -33 REMARK 465 GLU B -32 REMARK 465 GLN B -31 REMARK 465 LEU B -30 REMARK 465 ARG B -29 REMARK 465 ALA B -28 REMARK 465 ARG B -27 REMARK 465 LEU B -26 REMARK 465 GLU B -25 REMARK 465 HIS B -24 REMARK 465 HIS B -23 REMARK 465 PRO B -22 REMARK 465 GLN B -21 REMARK 465 GLY B -20 REMARK 465 GLN B -19 REMARK 465 ARG B -18 REMARK 465 GLU B -17 REMARK 465 PRO B -16 REMARK 465 ASP B -15 REMARK 465 TYR B -14 REMARK 465 ASP B -13 REMARK 465 ILE B -12 REMARK 465 PRO B -11 REMARK 465 THR B -10 REMARK 465 THR B -9 REMARK 465 GLU B -8 REMARK 465 ASN B -7 REMARK 465 LEU B -6 REMARK 465 TYR B -5 REMARK 465 PHE B -4 REMARK 465 GLN B -3 REMARK 465 GLY B -2 REMARK 465 THR B -1 REMARK 465 GLY B 0 REMARK 465 GLN B 1 REMARK 465 SER B 2 REMARK 465 VAL B 3 REMARK 465 ASN B 4 REMARK 465 ASP B 5 REMARK 465 PRO B 6 REMARK 465 GLY B 7 REMARK 465 ASN B 448 REMARK 465 THR C -1 REMARK 465 GLY C 0 REMARK 465 GLN C 1 REMARK 465 LYS C 2 REMARK 465 SER C 3 REMARK 465 ASP C 4 REMARK 465 ASP C 5 REMARK 465 ASP C 6 REMARK 465 TYR C 7 REMARK 465 GLU C 8 REMARK 465 ASP C 9 REMARK 465 TYR C 10 REMARK 465 THR C 11 REMARK 465 SER C 12 REMARK 465 ASN C 13 REMARK 465 LYS C 14 REMARK 465 THR C 15 REMARK 465 TRP C 16 REMARK 465 VAL C 17 REMARK 465 LEU C 18 REMARK 465 THR C 19 REMARK 465 PRO C 20 REMARK 465 LYS C 21 REMARK 465 VAL C 22 REMARK 465 PRO C 23 REMARK 465 GLU C 24 REMARK 465 THR C 430 REMARK 465 GLY C 431 REMARK 465 GLY C 432 REMARK 465 THR C 433 REMARK 465 THR C 434 REMARK 465 GLU C 435 REMARK 465 THR C 436 REMARK 465 SER C 437 REMARK 465 GLN C 438 REMARK 465 VAL C 439 REMARK 465 ALA C 440 REMARK 465 PRO C 441 REMARK 465 ALA C 442 REMARK 465 MET D -52 REMARK 465 ASP D -51 REMARK 465 GLU D -50 REMARK 465 LYS D -49 REMARK 465 THR D -48 REMARK 465 THR D -47 REMARK 465 GLY D -46 REMARK 465 TRP D -45 REMARK 465 ARG D -44 REMARK 465 GLY D -43 REMARK 465 GLY D -42 REMARK 465 HIS D -41 REMARK 465 VAL D -40 REMARK 465 VAL D -39 REMARK 465 GLU D -38 REMARK 465 GLY D -37 REMARK 465 LEU D -36 REMARK 465 ALA D -35 REMARK 465 GLY D -34 REMARK 465 GLU D -33 REMARK 465 LEU D -32 REMARK 465 GLU D -31 REMARK 465 GLN D -30 REMARK 465 LEU D -29 REMARK 465 ARG D -28 REMARK 465 ALA D -27 REMARK 465 ARG D -26 REMARK 465 LEU D -25 REMARK 465 GLU D -24 REMARK 465 HIS D -23 REMARK 465 HIS D -22 REMARK 465 PRO D -21 REMARK 465 GLN D -20 REMARK 465 GLY D -19 REMARK 465 GLN D -18 REMARK 465 ARG D -17 REMARK 465 GLU D -16 REMARK 465 PRO D -15 REMARK 465 ASP D -14 REMARK 465 TYR D -13 REMARK 465 ASP D -12 REMARK 465 ILE D -11 REMARK 465 PRO D -10 REMARK 465 THR D -9 REMARK 465 THR D -8 REMARK 465 GLU D -7 REMARK 465 ASN D -6 REMARK 465 LEU D -5 REMARK 465 TYR D -4 REMARK 465 PHE D -3 REMARK 465 GLN D -2 REMARK 465 GLY D -1 REMARK 465 THR D 0 REMARK 465 GLY D 1 REMARK 465 GLN D 2 REMARK 465 PRO D 3 REMARK 465 SER D 4 REMARK 465 GLN D 5 REMARK 465 ASP D 6 REMARK 465 GLU D 7 REMARK 465 LEU D 8 REMARK 465 LYS D 9 REMARK 465 GLU D 419 REMARK 465 PRO D 420 REMARK 465 GLN D 421 REMARK 465 LEU D 422 REMARK 465 LYS D 423 REMARK 465 ALA D 424 REMARK 465 PRO D 425 REMARK 465 THR D 426 REMARK 465 PRO D 427 REMARK 465 HIS D 428 REMARK 465 GLN D 429 REMARK 465 MET E -53 REMARK 465 ASP E -52 REMARK 465 GLU E -51 REMARK 465 LYS E -50 REMARK 465 THR E -49 REMARK 465 THR E -48 REMARK 465 GLY E -47 REMARK 465 TRP E -46 REMARK 465 ARG E -45 REMARK 465 GLY E -44 REMARK 465 GLY E -43 REMARK 465 HIS E -42 REMARK 465 VAL E -41 REMARK 465 VAL E -40 REMARK 465 GLU E -39 REMARK 465 GLY E -38 REMARK 465 LEU E -37 REMARK 465 ALA E -36 REMARK 465 GLY E -35 REMARK 465 GLU E -34 REMARK 465 LEU E -33 REMARK 465 GLU E -32 REMARK 465 GLN E -31 REMARK 465 LEU E -30 REMARK 465 ARG E -29 REMARK 465 ALA E -28 REMARK 465 ARG E -27 REMARK 465 LEU E -26 REMARK 465 GLU E -25 REMARK 465 HIS E -24 REMARK 465 HIS E -23 REMARK 465 PRO E -22 REMARK 465 GLN E -21 REMARK 465 GLY E -20 REMARK 465 GLN E -19 REMARK 465 ARG E -18 REMARK 465 GLU E -17 REMARK 465 PRO E -16 REMARK 465 ASP E -15 REMARK 465 TYR E -14 REMARK 465 ASP E -13 REMARK 465 ILE E -12 REMARK 465 PRO E -11 REMARK 465 THR E -10 REMARK 465 THR E -9 REMARK 465 GLU E -8 REMARK 465 ASN E -7 REMARK 465 LEU E -6 REMARK 465 TYR E -5 REMARK 465 PHE E -4 REMARK 465 GLN E -3 REMARK 465 GLY E -2 REMARK 465 THR E -1 REMARK 465 GLY E 0 REMARK 465 GLN E 1 REMARK 465 SER E 2 REMARK 465 VAL E 3 REMARK 465 ASN E 4 REMARK 465 ASP E 5 REMARK 465 PRO E 6 REMARK 465 GLY E 7 REMARK 465 ASN E 448 REMARK 465 HIS F 125 REMARK 465 HIS F 126 REMARK 465 HIS F 127 REMARK 465 HIS F 128 REMARK 465 HIS F 129 REMARK 465 HIS F 130 REMARK 465 GLU F 131 REMARK 465 PRO F 132 REMARK 465 GLU F 133 REMARK 465 ALA F 134 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 ASP A 98 52.67 -93.21 REMARK 500 LEU A 143 30.98 -98.03 REMARK 500 ARG A 173 -166.78 -124.39 REMARK 500 SER A 251 56.82 -91.31 REMARK 500 ASP B 95 52.45 -90.51 REMARK 500 ARG B 269 35.73 -99.21 REMARK 500 VAL C 48 -62.47 -121.07 REMARK 500 MET C 57 119.44 -160.05 REMARK 500 ASP C 110 58.62 -95.59 REMARK 500 PRO C 159 46.18 -97.25 REMARK 500 VAL C 204 -60.01 -120.86 REMARK 500 TYR C 427 30.43 -140.86 REMARK 500 SER D 49 116.62 -161.22 REMARK 500 ASP D 98 52.37 -92.76 REMARK 500 ARG D 173 -167.39 -124.14 REMARK 500 ASN D 248 -179.37 -179.27 REMARK 500 PRO E 78 47.30 -82.35 REMARK 500 ASP E 95 52.15 -91.22 REMARK 500 ALA E 246 52.80 -92.02 REMARK 500 PHE F 30 31.98 -97.84 REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: EMD-50379 RELATED DB: EMDB REMARK 900 CRYO-EM STRUCTURE OF THE ALPHA1BETA3GAMMA2 GABA(A) RECEPTOR IN REMARK 900 COMPLEX WITH GABA AND NB38 IN THE SHORT-LIVED ASYMMETRIC REMARK 900 DESENSITISED 1 STATE DBREF 9FFZ A 5 312 UNP P14867 GBRA1_HUMAN 32 339 DBREF 9FFZ A 391 429 UNP P14867 GBRA1_HUMAN 418 456 DBREF 9FFZ B 1 307 UNP P28472 GBRB3_HUMAN 26 332 DBREF 9FFZ B 422 448 UNP P28472 GBRB3_HUMAN 447 473 DBREF 9FFZ C 1 322 UNP P18507 GBRG2_HUMAN 40 361 DBREF 9FFZ C 400 428 UNP P18507 GBRG2_HUMAN 447 475 DBREF 9FFZ D 5 312 UNP P14867 GBRA1_HUMAN 32 339 DBREF 9FFZ D 391 429 UNP P14867 GBRA1_HUMAN 418 456 DBREF 9FFZ E 1 307 UNP P28472 GBRB3_HUMAN 26 332 DBREF 9FFZ E 422 448 UNP P28472 GBRB3_HUMAN 447 473 DBREF 9FFZ F 2 134 PDB 9FFZ 9FFZ 2 134 SEQADV 9FFZ MET A -52 UNP P14867 INITIATING METHIONINE SEQADV 9FFZ ASP A -51 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU A -50 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LYS A -49 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR A -48 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR A -47 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY A -46 UNP P14867 EXPRESSION TAG SEQADV 9FFZ TRP A -45 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ARG A -44 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY A -43 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY A -42 UNP P14867 EXPRESSION TAG SEQADV 9FFZ HIS A -41 UNP P14867 EXPRESSION TAG SEQADV 9FFZ VAL A -40 UNP P14867 EXPRESSION TAG SEQADV 9FFZ VAL A -39 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU A -38 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY A -37 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU A -36 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ALA A -35 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY A -34 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU A -33 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU A -32 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU A -31 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN A -30 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU A -29 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ARG A -28 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ALA A -27 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ARG A -26 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU A -25 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU A -24 UNP P14867 EXPRESSION TAG SEQADV 9FFZ HIS A -23 UNP P14867 EXPRESSION TAG SEQADV 9FFZ HIS A -22 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PRO A -21 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN A -20 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY A -19 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN A -18 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ARG A -17 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU A -16 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PRO A -15 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ASP A -14 UNP P14867 EXPRESSION TAG SEQADV 9FFZ TYR A -13 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ASP A -12 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ILE A -11 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PRO A -10 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR A -9 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR A -8 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU A -7 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ASN A -6 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU A -5 UNP P14867 EXPRESSION TAG SEQADV 9FFZ TYR A -4 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PHE A -3 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN A -2 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY A -1 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR A 0 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY A 1 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN A 2 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PRO A 3 UNP P14867 EXPRESSION TAG SEQADV 9FFZ SER A 4 UNP P14867 EXPRESSION TAG SEQADV 9FFZ SER A 313 UNP P14867 LINKER SEQADV 9FFZ GLN A 314 UNP P14867 LINKER SEQADV 9FFZ PRO A 315 UNP P14867 LINKER SEQADV 9FFZ ALA A 316 UNP P14867 LINKER SEQADV 9FFZ ARG A 317 UNP P14867 LINKER SEQADV 9FFZ ALA A 318 UNP P14867 LINKER SEQADV 9FFZ ALA A 319 UNP P14867 LINKER SEQADV 9FFZ MET B -53 UNP P28472 INITIATING METHIONINE SEQADV 9FFZ ASP B -52 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU B -51 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LYS B -50 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR B -49 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR B -48 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY B -47 UNP P28472 EXPRESSION TAG SEQADV 9FFZ TRP B -46 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ARG B -45 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY B -44 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY B -43 UNP P28472 EXPRESSION TAG SEQADV 9FFZ HIS B -42 UNP P28472 EXPRESSION TAG SEQADV 9FFZ VAL B -41 UNP P28472 EXPRESSION TAG SEQADV 9FFZ VAL B -40 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU B -39 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY B -38 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU B -37 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ALA B -36 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY B -35 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU B -34 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU B -33 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU B -32 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLN B -31 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU B -30 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ARG B -29 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ALA B -28 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ARG B -27 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU B -26 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU B -25 UNP P28472 EXPRESSION TAG SEQADV 9FFZ HIS B -24 UNP P28472 EXPRESSION TAG SEQADV 9FFZ HIS B -23 UNP P28472 EXPRESSION TAG SEQADV 9FFZ PRO B -22 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLN B -21 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY B -20 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLN B -19 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ARG B -18 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU B -17 UNP P28472 EXPRESSION TAG SEQADV 9FFZ PRO B -16 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ASP B -15 UNP P28472 EXPRESSION TAG SEQADV 9FFZ TYR B -14 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ASP B -13 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ILE B -12 UNP P28472 EXPRESSION TAG SEQADV 9FFZ PRO B -11 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR B -10 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR B -9 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU B -8 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ASN B -7 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU B -6 UNP P28472 EXPRESSION TAG SEQADV 9FFZ TYR B -5 UNP P28472 EXPRESSION TAG SEQADV 9FFZ PHE B -4 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLN B -3 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY B -2 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR B -1 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY B 0 UNP P28472 EXPRESSION TAG SEQADV 9FFZ SER B 308 UNP P28472 LINKER SEQADV 9FFZ GLN B 309 UNP P28472 LINKER SEQADV 9FFZ PRO B 310 UNP P28472 LINKER SEQADV 9FFZ ALA B 311 UNP P28472 LINKER SEQADV 9FFZ ARG B 312 UNP P28472 LINKER SEQADV 9FFZ ALA B 313 UNP P28472 LINKER SEQADV 9FFZ ALA B 314 UNP P28472 LINKER SEQADV 9FFZ THR C -1 UNP P18507 EXPRESSION TAG SEQADV 9FFZ GLY C 0 UNP P18507 EXPRESSION TAG SEQADV 9FFZ THR C 11 UNP P18507 ALA 50 CONFLICT SEQADV 9FFZ SER C 323 UNP P18507 LINKER SEQADV 9FFZ GLN C 324 UNP P18507 LINKER SEQADV 9FFZ PRO C 325 UNP P18507 LINKER SEQADV 9FFZ ALA C 326 UNP P18507 LINKER SEQADV 9FFZ ARG C 327 UNP P18507 LINKER SEQADV 9FFZ ALA C 328 UNP P18507 LINKER SEQADV 9FFZ GLY C 429 UNP P18507 EXPRESSION TAG SEQADV 9FFZ THR C 430 UNP P18507 EXPRESSION TAG SEQADV 9FFZ GLY C 431 UNP P18507 EXPRESSION TAG SEQADV 9FFZ GLY C 432 UNP P18507 EXPRESSION TAG SEQADV 9FFZ THR C 433 UNP P18507 EXPRESSION TAG SEQADV 9FFZ THR C 434 UNP P18507 EXPRESSION TAG SEQADV 9FFZ GLU C 435 UNP P18507 EXPRESSION TAG SEQADV 9FFZ THR C 436 UNP P18507 EXPRESSION TAG SEQADV 9FFZ SER C 437 UNP P18507 EXPRESSION TAG SEQADV 9FFZ GLN C 438 UNP P18507 EXPRESSION TAG SEQADV 9FFZ VAL C 439 UNP P18507 EXPRESSION TAG SEQADV 9FFZ ALA C 440 UNP P18507 EXPRESSION TAG SEQADV 9FFZ PRO C 441 UNP P18507 EXPRESSION TAG SEQADV 9FFZ ALA C 442 UNP P18507 EXPRESSION TAG SEQADV 9FFZ MET D -52 UNP P14867 INITIATING METHIONINE SEQADV 9FFZ ASP D -51 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU D -50 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LYS D -49 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR D -48 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR D -47 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY D -46 UNP P14867 EXPRESSION TAG SEQADV 9FFZ TRP D -45 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ARG D -44 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY D -43 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY D -42 UNP P14867 EXPRESSION TAG SEQADV 9FFZ HIS D -41 UNP P14867 EXPRESSION TAG SEQADV 9FFZ VAL D -40 UNP P14867 EXPRESSION TAG SEQADV 9FFZ VAL D -39 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU D -38 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY D -37 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU D -36 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ALA D -35 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY D -34 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU D -33 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU D -32 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU D -31 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN D -30 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU D -29 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ARG D -28 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ALA D -27 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ARG D -26 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU D -25 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU D -24 UNP P14867 EXPRESSION TAG SEQADV 9FFZ HIS D -23 UNP P14867 EXPRESSION TAG SEQADV 9FFZ HIS D -22 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PRO D -21 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN D -20 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY D -19 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN D -18 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ARG D -17 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU D -16 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PRO D -15 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ASP D -14 UNP P14867 EXPRESSION TAG SEQADV 9FFZ TYR D -13 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ASP D -12 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ILE D -11 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PRO D -10 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR D -9 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR D -8 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLU D -7 UNP P14867 EXPRESSION TAG SEQADV 9FFZ ASN D -6 UNP P14867 EXPRESSION TAG SEQADV 9FFZ LEU D -5 UNP P14867 EXPRESSION TAG SEQADV 9FFZ TYR D -4 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PHE D -3 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN D -2 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY D -1 UNP P14867 EXPRESSION TAG SEQADV 9FFZ THR D 0 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLY D 1 UNP P14867 EXPRESSION TAG SEQADV 9FFZ GLN D 2 UNP P14867 EXPRESSION TAG SEQADV 9FFZ PRO D 3 UNP P14867 EXPRESSION TAG SEQADV 9FFZ SER D 4 UNP P14867 EXPRESSION TAG SEQADV 9FFZ SER D 313 UNP P14867 LINKER SEQADV 9FFZ GLN D 314 UNP P14867 LINKER SEQADV 9FFZ PRO D 315 UNP P14867 LINKER SEQADV 9FFZ ALA D 316 UNP P14867 LINKER SEQADV 9FFZ ARG D 317 UNP P14867 LINKER SEQADV 9FFZ ALA D 318 UNP P14867 LINKER SEQADV 9FFZ ALA D 319 UNP P14867 LINKER SEQADV 9FFZ MET E -53 UNP P28472 INITIATING METHIONINE SEQADV 9FFZ ASP E -52 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU E -51 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LYS E -50 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR E -49 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR E -48 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY E -47 UNP P28472 EXPRESSION TAG SEQADV 9FFZ TRP E -46 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ARG E -45 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY E -44 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY E -43 UNP P28472 EXPRESSION TAG SEQADV 9FFZ HIS E -42 UNP P28472 EXPRESSION TAG SEQADV 9FFZ VAL E -41 UNP P28472 EXPRESSION TAG SEQADV 9FFZ VAL E -40 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU E -39 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY E -38 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU E -37 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ALA E -36 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY E -35 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU E -34 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU E -33 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU E -32 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLN E -31 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU E -30 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ARG E -29 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ALA E -28 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ARG E -27 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU E -26 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU E -25 UNP P28472 EXPRESSION TAG SEQADV 9FFZ HIS E -24 UNP P28472 EXPRESSION TAG SEQADV 9FFZ HIS E -23 UNP P28472 EXPRESSION TAG SEQADV 9FFZ PRO E -22 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLN E -21 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY E -20 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLN E -19 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ARG E -18 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU E -17 UNP P28472 EXPRESSION TAG SEQADV 9FFZ PRO E -16 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ASP E -15 UNP P28472 EXPRESSION TAG SEQADV 9FFZ TYR E -14 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ASP E -13 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ILE E -12 UNP P28472 EXPRESSION TAG SEQADV 9FFZ PRO E -11 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR E -10 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR E -9 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLU E -8 UNP P28472 EXPRESSION TAG SEQADV 9FFZ ASN E -7 UNP P28472 EXPRESSION TAG SEQADV 9FFZ LEU E -6 UNP P28472 EXPRESSION TAG SEQADV 9FFZ TYR E -5 UNP P28472 EXPRESSION TAG SEQADV 9FFZ PHE E -4 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLN E -3 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY E -2 UNP P28472 EXPRESSION TAG SEQADV 9FFZ THR E -1 UNP P28472 EXPRESSION TAG SEQADV 9FFZ GLY E 0 UNP P28472 EXPRESSION TAG SEQADV 9FFZ SER E 308 UNP P28472 LINKER SEQADV 9FFZ GLN E 309 UNP P28472 LINKER SEQADV 9FFZ PRO E 310 UNP P28472 LINKER SEQADV 9FFZ ALA E 311 UNP P28472 LINKER SEQADV 9FFZ ARG E 312 UNP P28472 LINKER SEQADV 9FFZ ALA E 313 UNP P28472 LINKER SEQADV 9FFZ ALA E 314 UNP P28472 LINKER SEQRES 1 A 411 MET ASP GLU LYS THR THR GLY TRP ARG GLY GLY HIS VAL SEQRES 2 A 411 VAL GLU GLY LEU ALA GLY GLU LEU GLU GLN LEU ARG ALA SEQRES 3 A 411 ARG LEU GLU HIS HIS PRO GLN GLY GLN ARG GLU PRO ASP SEQRES 4 A 411 TYR ASP ILE PRO THR THR GLU ASN LEU TYR PHE GLN GLY SEQRES 5 A 411 THR GLY GLN PRO SER GLN ASP GLU LEU LYS ASP ASN THR SEQRES 6 A 411 THR VAL PHE THR ARG ILE LEU ASP ARG LEU LEU ASP GLY SEQRES 7 A 411 TYR ASP ASN ARG LEU ARG PRO GLY LEU GLY GLU ARG VAL SEQRES 8 A 411 THR GLU VAL LYS THR ASP ILE PHE VAL THR SER PHE GLY SEQRES 9 A 411 PRO VAL SER ASP HIS ASP MET GLU TYR THR ILE ASP VAL SEQRES 10 A 411 PHE PHE ARG GLN SER TRP LYS ASP GLU ARG LEU LYS PHE SEQRES 11 A 411 LYS GLY PRO MET THR VAL LEU ARG LEU ASN ASN LEU MET SEQRES 12 A 411 ALA SER LYS ILE TRP THR PRO ASP THR PHE PHE HIS ASN SEQRES 13 A 411 GLY LYS LYS SER VAL ALA HIS ASN MET THR MET PRO ASN SEQRES 14 A 411 LYS LEU LEU ARG ILE THR GLU ASP GLY THR LEU LEU TYR SEQRES 15 A 411 THR MET ARG LEU THR VAL ARG ALA GLU CYS PRO MET HIS SEQRES 16 A 411 LEU GLU ASP PHE PRO MET ASP ALA HIS ALA CYS PRO LEU SEQRES 17 A 411 LYS PHE GLY SER TYR ALA TYR THR ARG ALA GLU VAL VAL SEQRES 18 A 411 TYR GLU TRP THR ARG GLU PRO ALA ARG SER VAL VAL VAL SEQRES 19 A 411 ALA GLU ASP GLY SER ARG LEU ASN GLN TYR ASP LEU LEU SEQRES 20 A 411 GLY GLN THR VAL ASP SER GLY ILE VAL GLN SER SER THR SEQRES 21 A 411 GLY GLU TYR VAL VAL MET THR THR HIS PHE HIS LEU LYS SEQRES 22 A 411 ARG LYS ILE GLY TYR PHE VAL ILE GLN THR TYR LEU PRO SEQRES 23 A 411 CYS ILE MET THR VAL ILE LEU SER GLN VAL SER PHE TRP SEQRES 24 A 411 LEU ASN ARG GLU SER VAL PRO ALA ARG THR VAL PHE GLY SEQRES 25 A 411 VAL THR THR VAL LEU THR MET THR THR LEU SER ILE SER SEQRES 26 A 411 ALA ARG ASN SER LEU PRO LYS VAL ALA TYR ALA THR ALA SEQRES 27 A 411 MET ASP TRP PHE ILE ALA VAL CYS TYR ALA PHE VAL PHE SEQRES 28 A 411 SER ALA LEU ILE GLU PHE ALA THR VAL ASN TYR PHE THR SEQRES 29 A 411 LYS SER GLN PRO ALA ARG ALA ALA LYS ILE ASP ARG LEU SEQRES 30 A 411 SER ARG ILE ALA PHE PRO LEU LEU PHE GLY ILE PHE ASN SEQRES 31 A 411 LEU VAL TYR TRP ALA THR TYR LEU ASN ARG GLU PRO GLN SEQRES 32 A 411 LEU LYS ALA PRO THR PRO HIS GLN SEQRES 1 B 395 MET ASP GLU LYS THR THR GLY TRP ARG GLY GLY HIS VAL SEQRES 2 B 395 VAL GLU GLY LEU ALA GLY GLU LEU GLU GLN LEU ARG ALA SEQRES 3 B 395 ARG LEU GLU HIS HIS PRO GLN GLY GLN ARG GLU PRO ASP SEQRES 4 B 395 TYR ASP ILE PRO THR THR GLU ASN LEU TYR PHE GLN GLY SEQRES 5 B 395 THR GLY GLN SER VAL ASN ASP PRO GLY ASN MET SER PHE SEQRES 6 B 395 VAL LYS GLU THR VAL ASP LYS LEU LEU LYS GLY TYR ASP SEQRES 7 B 395 ILE ARG LEU ARG PRO ASP PHE GLY GLY PRO PRO VAL CYS SEQRES 8 B 395 VAL GLY MET ASN ILE ASP ILE ALA SER ILE ASP MET VAL SEQRES 9 B 395 SER GLU VAL ASN MET ASP TYR THR LEU THR MET TYR PHE SEQRES 10 B 395 GLN GLN TYR TRP ARG ASP LYS ARG LEU ALA TYR SER GLY SEQRES 11 B 395 ILE PRO LEU ASN LEU THR LEU ASP ASN ARG VAL ALA ASP SEQRES 12 B 395 GLN LEU TRP VAL PRO ASP THR TYR PHE LEU ASN ASP LYS SEQRES 13 B 395 LYS SER PHE VAL HIS GLY VAL THR VAL LYS ASN ARG MET SEQRES 14 B 395 ILE ARG LEU HIS PRO ASP GLY THR VAL LEU TYR GLY LEU SEQRES 15 B 395 ARG ILE THR THR THR ALA ALA CYS MET MET ASP LEU ARG SEQRES 16 B 395 ARG TYR PRO LEU ASP GLU GLN ASN CYS THR LEU GLU ILE SEQRES 17 B 395 GLU SER TYR GLY TYR THR THR ASP ASP ILE GLU PHE TYR SEQRES 18 B 395 TRP ARG GLY GLY ASP LYS ALA VAL THR GLY VAL GLU ARG SEQRES 19 B 395 ILE GLU LEU PRO GLN PHE SER ILE VAL GLU HIS ARG LEU SEQRES 20 B 395 VAL SER ARG ASN VAL VAL PHE ALA THR GLY ALA TYR PRO SEQRES 21 B 395 ARG LEU SER LEU SER PHE ARG LEU LYS ARG ASN ILE GLY SEQRES 22 B 395 TYR PHE ILE LEU GLN THR TYR MET PRO SER ILE LEU ILE SEQRES 23 B 395 THR ILE LEU SER TRP VAL SER PHE TRP ILE ASN TYR ASP SEQRES 24 B 395 ALA SER ALA ALA ARG VAL ALA LEU GLY ILE THR THR VAL SEQRES 25 B 395 LEU THR MET THR THR ILE ASN THR HIS LEU ARG GLU THR SEQRES 26 B 395 LEU PRO LYS ILE PRO TYR VAL LYS ALA ILE ASP MET TYR SEQRES 27 B 395 LEU MET GLY CYS PHE VAL PHE VAL PHE LEU ALA LEU LEU SEQRES 28 B 395 GLU TYR ALA PHE VAL ASN TYR ILE PHE PHE SER GLN PRO SEQRES 29 B 395 ALA ARG ALA ALA ALA ILE ASP ARG TRP SER ARG ILE VAL SEQRES 30 B 395 PHE PRO PHE THR PHE SER LEU PHE ASN LEU VAL TYR TRP SEQRES 31 B 395 LEU TYR TYR VAL ASN SEQRES 1 C 373 THR GLY GLN LYS SER ASP ASP ASP TYR GLU ASP TYR THR SEQRES 2 C 373 SER ASN LYS THR TRP VAL LEU THR PRO LYS VAL PRO GLU SEQRES 3 C 373 GLY ASP VAL THR VAL ILE LEU ASN ASN LEU LEU GLU GLY SEQRES 4 C 373 TYR ASP ASN LYS LEU ARG PRO ASP ILE GLY VAL LYS PRO SEQRES 5 C 373 THR LEU ILE HIS THR ASP MET TYR VAL ASN SER ILE GLY SEQRES 6 C 373 PRO VAL ASN ALA ILE ASN MET GLU TYR THR ILE ASP ILE SEQRES 7 C 373 PHE PHE ALA GLN THR TRP TYR ASP ARG ARG LEU LYS PHE SEQRES 8 C 373 ASN SER THR ILE LYS VAL LEU ARG LEU ASN SER ASN MET SEQRES 9 C 373 VAL GLY LYS ILE TRP ILE PRO ASP THR PHE PHE ARG ASN SEQRES 10 C 373 SER LYS LYS ALA ASP ALA HIS TRP ILE THR THR PRO ASN SEQRES 11 C 373 ARG MET LEU ARG ILE TRP ASN ASP GLY ARG VAL LEU TYR SEQRES 12 C 373 THR LEU ARG LEU THR ILE ASP ALA GLU CYS GLN LEU GLN SEQRES 13 C 373 LEU HIS ASN PHE PRO MET ASP GLU HIS SER CYS PRO LEU SEQRES 14 C 373 GLU PHE SER SER TYR GLY TYR PRO ARG GLU GLU ILE VAL SEQRES 15 C 373 TYR GLN TRP LYS ARG SER SER VAL GLU VAL GLY ASP THR SEQRES 16 C 373 ARG SER TRP ARG LEU TYR GLN PHE SER PHE VAL GLY LEU SEQRES 17 C 373 ARG ASN THR THR GLU VAL VAL LYS THR THR SER GLY ASP SEQRES 18 C 373 TYR VAL VAL MET SER VAL TYR PHE ASP LEU SER ARG ARG SEQRES 19 C 373 MET GLY TYR PHE THR ILE GLN THR TYR ILE PRO CYS THR SEQRES 20 C 373 LEU ILE VAL VAL LEU SER TRP VAL SER PHE TRP ILE ASN SEQRES 21 C 373 LYS ASP ALA VAL PRO ALA ARG THR SER LEU GLY ILE THR SEQRES 22 C 373 THR VAL LEU THR MET THR THR LEU SER THR ILE ALA ARG SEQRES 23 C 373 LYS SER LEU PRO LYS VAL SER TYR VAL THR ALA MET ASP SEQRES 24 C 373 LEU PHE VAL SER VAL CYS PHE ILE PHE VAL PHE SER ALA SEQRES 25 C 373 LEU VAL GLU TYR GLY THR LEU HIS TYR PHE VAL SER SER SEQRES 26 C 373 GLN PRO ALA ARG ALA ALA LYS MET ASP SER TYR ALA ARG SEQRES 27 C 373 ILE PHE PHE PRO THR ALA PHE CYS LEU PHE ASN LEU VAL SEQRES 28 C 373 TYR TRP VAL SER TYR LEU TYR LEU GLY THR GLY GLY THR SEQRES 29 C 373 THR GLU THR SER GLN VAL ALA PRO ALA SEQRES 1 D 411 MET ASP GLU LYS THR THR GLY TRP ARG GLY GLY HIS VAL SEQRES 2 D 411 VAL GLU GLY LEU ALA GLY GLU LEU GLU GLN LEU ARG ALA SEQRES 3 D 411 ARG LEU GLU HIS HIS PRO GLN GLY GLN ARG GLU PRO ASP SEQRES 4 D 411 TYR ASP ILE PRO THR THR GLU ASN LEU TYR PHE GLN GLY SEQRES 5 D 411 THR GLY GLN PRO SER GLN ASP GLU LEU LYS ASP ASN THR SEQRES 6 D 411 THR VAL PHE THR ARG ILE LEU ASP ARG LEU LEU ASP GLY SEQRES 7 D 411 TYR ASP ASN ARG LEU ARG PRO GLY LEU GLY GLU ARG VAL SEQRES 8 D 411 THR GLU VAL LYS THR ASP ILE PHE VAL THR SER PHE GLY SEQRES 9 D 411 PRO VAL SER ASP HIS ASP MET GLU TYR THR ILE ASP VAL SEQRES 10 D 411 PHE PHE ARG GLN SER TRP LYS ASP GLU ARG LEU LYS PHE SEQRES 11 D 411 LYS GLY PRO MET THR VAL LEU ARG LEU ASN ASN LEU MET SEQRES 12 D 411 ALA SER LYS ILE TRP THR PRO ASP THR PHE PHE HIS ASN SEQRES 13 D 411 GLY LYS LYS SER VAL ALA HIS ASN MET THR MET PRO ASN SEQRES 14 D 411 LYS LEU LEU ARG ILE THR GLU ASP GLY THR LEU LEU TYR SEQRES 15 D 411 THR MET ARG LEU THR VAL ARG ALA GLU CYS PRO MET HIS SEQRES 16 D 411 LEU GLU ASP PHE PRO MET ASP ALA HIS ALA CYS PRO LEU SEQRES 17 D 411 LYS PHE GLY SER TYR ALA TYR THR ARG ALA GLU VAL VAL SEQRES 18 D 411 TYR GLU TRP THR ARG GLU PRO ALA ARG SER VAL VAL VAL SEQRES 19 D 411 ALA GLU ASP GLY SER ARG LEU ASN GLN TYR ASP LEU LEU SEQRES 20 D 411 GLY GLN THR VAL ASP SER GLY ILE VAL GLN SER SER THR SEQRES 21 D 411 GLY GLU TYR VAL VAL MET THR THR HIS PHE HIS LEU LYS SEQRES 22 D 411 ARG LYS ILE GLY TYR PHE VAL ILE GLN THR TYR LEU PRO SEQRES 23 D 411 CYS ILE MET THR VAL ILE LEU SER GLN VAL SER PHE TRP SEQRES 24 D 411 LEU ASN ARG GLU SER VAL PRO ALA ARG THR VAL PHE GLY SEQRES 25 D 411 VAL THR THR VAL LEU THR MET THR THR LEU SER ILE SER SEQRES 26 D 411 ALA ARG ASN SER LEU PRO LYS VAL ALA TYR ALA THR ALA SEQRES 27 D 411 MET ASP TRP PHE ILE ALA VAL CYS TYR ALA PHE VAL PHE SEQRES 28 D 411 SER ALA LEU ILE GLU PHE ALA THR VAL ASN TYR PHE THR SEQRES 29 D 411 LYS SER GLN PRO ALA ARG ALA ALA LYS ILE ASP ARG LEU SEQRES 30 D 411 SER ARG ILE ALA PHE PRO LEU LEU PHE GLY ILE PHE ASN SEQRES 31 D 411 LEU VAL TYR TRP ALA THR TYR LEU ASN ARG GLU PRO GLN SEQRES 32 D 411 LEU LYS ALA PRO THR PRO HIS GLN SEQRES 1 E 395 MET ASP GLU LYS THR THR GLY TRP ARG GLY GLY HIS VAL SEQRES 2 E 395 VAL GLU GLY LEU ALA GLY GLU LEU GLU GLN LEU ARG ALA SEQRES 3 E 395 ARG LEU GLU HIS HIS PRO GLN GLY GLN ARG GLU PRO ASP SEQRES 4 E 395 TYR ASP ILE PRO THR THR GLU ASN LEU TYR PHE GLN GLY SEQRES 5 E 395 THR GLY GLN SER VAL ASN ASP PRO GLY ASN MET SER PHE SEQRES 6 E 395 VAL LYS GLU THR VAL ASP LYS LEU LEU LYS GLY TYR ASP SEQRES 7 E 395 ILE ARG LEU ARG PRO ASP PHE GLY GLY PRO PRO VAL CYS SEQRES 8 E 395 VAL GLY MET ASN ILE ASP ILE ALA SER ILE ASP MET VAL SEQRES 9 E 395 SER GLU VAL ASN MET ASP TYR THR LEU THR MET TYR PHE SEQRES 10 E 395 GLN GLN TYR TRP ARG ASP LYS ARG LEU ALA TYR SER GLY SEQRES 11 E 395 ILE PRO LEU ASN LEU THR LEU ASP ASN ARG VAL ALA ASP SEQRES 12 E 395 GLN LEU TRP VAL PRO ASP THR TYR PHE LEU ASN ASP LYS SEQRES 13 E 395 LYS SER PHE VAL HIS GLY VAL THR VAL LYS ASN ARG MET SEQRES 14 E 395 ILE ARG LEU HIS PRO ASP GLY THR VAL LEU TYR GLY LEU SEQRES 15 E 395 ARG ILE THR THR THR ALA ALA CYS MET MET ASP LEU ARG SEQRES 16 E 395 ARG TYR PRO LEU ASP GLU GLN ASN CYS THR LEU GLU ILE SEQRES 17 E 395 GLU SER TYR GLY TYR THR THR ASP ASP ILE GLU PHE TYR SEQRES 18 E 395 TRP ARG GLY GLY ASP LYS ALA VAL THR GLY VAL GLU ARG SEQRES 19 E 395 ILE GLU LEU PRO GLN PHE SER ILE VAL GLU HIS ARG LEU SEQRES 20 E 395 VAL SER ARG ASN VAL VAL PHE ALA THR GLY ALA TYR PRO SEQRES 21 E 395 ARG LEU SER LEU SER PHE ARG LEU LYS ARG ASN ILE GLY SEQRES 22 E 395 TYR PHE ILE LEU GLN THR TYR MET PRO SER ILE LEU ILE SEQRES 23 E 395 THR ILE LEU SER TRP VAL SER PHE TRP ILE ASN TYR ASP SEQRES 24 E 395 ALA SER ALA ALA ARG VAL ALA LEU GLY ILE THR THR VAL SEQRES 25 E 395 LEU THR MET THR THR ILE ASN THR HIS LEU ARG GLU THR SEQRES 26 E 395 LEU PRO LYS ILE PRO TYR VAL LYS ALA ILE ASP MET TYR SEQRES 27 E 395 LEU MET GLY CYS PHE VAL PHE VAL PHE LEU ALA LEU LEU SEQRES 28 E 395 GLU TYR ALA PHE VAL ASN TYR ILE PHE PHE SER GLN PRO SEQRES 29 E 395 ALA ARG ALA ALA ALA ILE ASP ARG TRP SER ARG ILE VAL SEQRES 30 E 395 PHE PRO PHE THR PHE SER LEU PHE ASN LEU VAL TYR TRP SEQRES 31 E 395 LEU TYR TYR VAL ASN SEQRES 1 F 133 GLN VAL GLN LEU GLN GLU SER GLY GLY GLY LEU VAL GLN SEQRES 2 F 133 ALA GLY GLY SER LEU ARG VAL SER CYS ALA ALA SER GLY SEQRES 3 F 133 ARG THR PHE THR THR TYR ILE MET ALA TRP PHE ARG GLN SEQRES 4 F 133 ALA PRO GLY LYS GLU ARG GLU PHE LEU ALA ALA MET ASP SEQRES 5 F 133 GLN GLY ARG ILE GLN TYR TYR GLY ASP SER VAL ARG GLY SEQRES 6 F 133 ARG PHE THR ILE SER ARG ASP TYR ALA LYS ASN SER VAL SEQRES 7 F 133 ASP LEU GLN LEU ASP GLY LEU ARG PRO GLU ASP THR ALA SEQRES 8 F 133 VAL TYR TYR CYS ALA ALA GLY ALA GLY PHE TRP GLY LEU SEQRES 9 F 133 ARG THR ALA SER SER TYR HIS TYR TRP GLY GLN GLY THR SEQRES 10 F 133 GLN VAL THR VAL SER SER HIS HIS HIS HIS HIS HIS GLU SEQRES 11 F 133 PRO GLU ALA HET NAG a 1 14 HET NAG a 2 14 HET BMA a 3 11 HET MAN a 4 11 HET MAN a 5 11 HET MAN a 6 11 HET MAN a 7 11 HET MAN a 8 11 HET MAN a 9 11 HET MAN a 10 11 HET NAG b 1 14 HET NAG b 2 14 HET NAG G 1 14 HET NAG G 2 14 HET BMA G 3 11 HET MAN G 4 11 HET MAN G 5 11 HET MAN G 6 11 HET NAG c 1 14 HET NAG c 2 14 HET NAG d 1 14 HET NAG d 2 14 HET BMA d 3 11 HET MAN d 4 11 HET MAN d 5 11 HET NAG e 1 14 HET NAG e 2 14 HET NAG H 1 14 HET NAG H 2 14 HET BMA H 3 11 HET MAN H 4 11 HET MAN H 5 11 HET MAN H 6 11 HET ABU A 501 7 HET D3D A 502 51 HET CLR B 501 28 HET ABU E 501 7 HETNAM NAG 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSE HETNAM BMA BETA-D-MANNOPYRANOSE HETNAM MAN ALPHA-D-MANNOPYRANOSE HETNAM ABU GAMMA-AMINO-BUTANOIC ACID HETNAM D3D (19S,22R,25R)-22,25,26-TRIHYDROXY-16,22-DIOXO-17,21,23- HETNAM 2 D3D TRIOXA-22LAMBDA~5~-PHOSPHAHEXACOSAN-19-YL (9E)- HETNAM 3 D3D OCTADEC-9-ENOATE HETNAM CLR CHOLESTEROL HETSYN NAG N-ACETYL-BETA-D-GLUCOSAMINE; 2-ACETAMIDO-2-DEOXY-BETA- HETSYN 2 NAG D-GLUCOSE; 2-ACETAMIDO-2-DEOXY-D-GLUCOSE; 2-ACETAMIDO- HETSYN 3 NAG 2-DEOXY-GLUCOSE; N-ACETYL-D-GLUCOSAMINE HETSYN BMA BETA-D-MANNOSE; D-MANNOSE; MANNOSE HETSYN MAN ALPHA-D-MANNOSE; D-MANNOSE; MANNOSE HETSYN ABU GAMMA(AMINO)-BUTYRIC ACID FORMUL 7 NAG 14(C8 H15 N O6) FORMUL 7 BMA 4(C6 H12 O6) FORMUL 7 MAN 15(C6 H12 O6) FORMUL 14 ABU 2(C4 H9 N O2) FORMUL 15 D3D C40 H77 O10 P FORMUL 16 CLR C27 H46 O HELIX 1 AA1 ASP A 10 LEU A 23 1 14 HELIX 2 AA2 GLU A 73 LYS A 76 5 4 HELIX 3 AA3 ASN A 87 ILE A 94 5 8 HELIX 4 AA4 GLU A 174 ARG A 177 5 4 HELIX 5 AA5 ILE A 223 THR A 230 1 8 HELIX 6 AA6 THR A 230 GLN A 242 1 13 HELIX 7 AA7 VAL A 243 TRP A 246 5 4 HELIX 8 AA8 SER A 251 LEU A 277 1 27 HELIX 9 AA9 THR A 284 THR A 311 1 28 HELIX 10 AB1 GLN A 314 ASN A 417 1 33 HELIX 11 AB2 MET B 9 LYS B 21 1 13 HELIX 12 AB3 LYS B 70 ALA B 73 5 4 HELIX 13 AB4 ASP B 84 LEU B 91 5 8 HELIX 14 AB5 GLY B 170 LYS B 173 5 4 HELIX 15 AB6 ILE B 218 LEU B 223 1 6 HELIX 16 AB7 THR B 225 TRP B 237 1 13 HELIX 17 AB8 VAL B 238 ILE B 242 5 5 HELIX 18 AB9 ALA B 246 ARG B 269 1 24 HELIX 19 AC1 LYS B 279 PHE B 306 1 28 HELIX 20 AC2 GLN B 309 VAL B 447 1 32 HELIX 21 AC3 ASP C 26 LEU C 35 1 10 HELIX 22 AC4 ASP C 84 LYS C 88 5 5 HELIX 23 AC5 ASN C 99 VAL C 103 5 5 HELIX 24 AC6 GLN C 154 PHE C 158 5 5 HELIX 25 AC7 MET C 233 THR C 240 1 8 HELIX 26 AC8 THR C 240 TRP C 252 1 13 HELIX 27 AC9 TRP C 252 ILE C 257 1 6 HELIX 28 AD1 ALA C 261 LEU C 287 1 27 HELIX 29 AD2 THR C 294 GLN C 324 1 31 HELIX 30 AD3 GLN C 324 TYR C 427 1 33 HELIX 31 AD4 ASN D 11 LEU D 23 1 13 HELIX 32 AD5 GLU D 73 LYS D 76 5 4 HELIX 33 AD6 ASN D 87 ILE D 94 5 8 HELIX 34 AD7 GLU D 174 ARG D 177 5 4 HELIX 35 AD8 ILE D 223 THR D 230 1 8 HELIX 36 AD9 THR D 230 GLN D 242 1 13 HELIX 37 AE1 VAL D 243 LEU D 247 5 5 HELIX 38 AE2 SER D 251 ALA D 273 1 23 HELIX 39 AE3 THR D 284 LYS D 312 1 29 HELIX 40 AE4 GLN D 314 ARG D 418 1 34 HELIX 41 AE5 MET E 9 LYS E 21 1 13 HELIX 42 AE6 LYS E 70 ALA E 73 5 4 HELIX 43 AE7 ASP E 84 GLN E 90 5 7 HELIX 44 AE8 ASP E 139 TYR E 143 5 5 HELIX 45 AE9 GLY E 170 LYS E 173 5 4 HELIX 46 AF1 ILE E 218 THR E 225 1 8 HELIX 47 AF2 THR E 225 VAL E 238 1 14 HELIX 48 AF3 SER E 239 TRP E 241 5 3 HELIX 49 AF4 ASN E 243 ASP E 245 5 3 HELIX 50 AF5 ALA E 246 THR E 271 1 26 HELIX 51 AF6 LYS E 279 PHE E 306 1 28 HELIX 52 AF7 GLN E 309 VAL E 447 1 32 HELIX 53 AF8 ARG F 87 THR F 91 5 5 HELIX 54 AF9 THR F 107 TYR F 111 5 5 SHEET 1 AA1 6 VAL A 83 LEU A 86 0 SHEET 2 AA1 6 LYS A 117 THR A 122 -1 O LEU A 119 N LEU A 86 SHEET 3 AA1 6 THR A 126 GLU A 138 -1 O THR A 126 N THR A 122 SHEET 4 AA1 6 GLU A 59 LYS A 71 -1 N GLN A 68 O TYR A 129 SHEET 5 AA1 6 THR A 39 SER A 54 -1 N LYS A 42 O SER A 69 SHEET 6 AA1 6 VAL A 167 TRP A 171 1 O GLU A 170 N VAL A 41 SHEET 1 AA2 5 VAL A 108 ALA A 109 0 SHEET 2 AA2 5 THR A 126 GLU A 138 -1 O THR A 134 N VAL A 108 SHEET 3 AA2 5 GLU A 59 LYS A 71 -1 N GLN A 68 O TYR A 129 SHEET 4 AA2 5 THR A 39 SER A 54 -1 N LYS A 42 O SER A 69 SHEET 5 AA2 5 VAL A 179 VAL A 181 1 O VAL A 180 N VAL A 47 SHEET 1 AA3 4 THR A 99 PHE A 101 0 SHEET 2 AA3 4 PHE A 157 SER A 159 -1 O GLY A 158 N PHE A 100 SHEET 3 AA3 4 GLU A 209 LYS A 220 -1 O MET A 213 N PHE A 157 SHEET 4 AA3 4 ALA A 150 PRO A 154 -1 N HIS A 151 O LEU A 219 SHEET 1 AA4 4 THR A 99 PHE A 101 0 SHEET 2 AA4 4 PHE A 157 SER A 159 -1 O GLY A 158 N PHE A 100 SHEET 3 AA4 4 GLU A 209 LYS A 220 -1 O MET A 213 N PHE A 157 SHEET 4 AA4 4 LEU A 193 GLN A 204 -1 N GLY A 201 O VAL A 212 SHEET 1 AA5 6 THR B 82 LEU B 83 0 SHEET 2 AA5 6 ARG B 114 LEU B 118 -1 O ILE B 116 N LEU B 83 SHEET 3 AA5 6 THR B 123 LEU B 128 -1 O GLY B 127 N MET B 115 SHEET 4 AA5 6 ASP B 56 ARG B 68 -1 N PHE B 63 O LEU B 128 SHEET 5 AA5 6 VAL B 36 SER B 51 -1 N GLY B 39 O TYR B 66 SHEET 6 AA5 6 ILE B 164 TRP B 168 1 O GLU B 165 N VAL B 38 SHEET 1 AA6 5 ASP B 101 VAL B 106 0 SHEET 2 AA6 5 ILE B 130 ALA B 135 -1 O THR B 131 N PHE B 105 SHEET 3 AA6 5 ASP B 56 ARG B 68 -1 N LEU B 59 O THR B 132 SHEET 4 AA6 5 VAL B 36 SER B 51 -1 N GLY B 39 O TYR B 66 SHEET 5 AA6 5 VAL B 175 THR B 176 1 O THR B 176 N ILE B 44 SHEET 1 AA7 4 THR B 96 PHE B 98 0 SHEET 2 AA7 4 GLU B 147 SER B 156 -1 O GLU B 155 N TYR B 97 SHEET 3 AA7 4 GLY B 203 ARG B 216 -1 O LEU B 208 N ILE B 154 SHEET 4 AA7 4 PHE B 186 PHE B 200 -1 N PHE B 200 O GLY B 203 SHEET 1 AA8 4 VAL C 95 LEU C 98 0 SHEET 2 AA8 4 ARG C 129 TRP C 134 -1 O ILE C 133 N LEU C 96 SHEET 3 AA8 4 VAL C 139 GLU C 150 -1 O LEU C 140 N ARG C 132 SHEET 4 AA8 4 SER C 116 ALA C 121 -1 N LYS C 117 O ASP C 148 SHEET 1 AA9 6 VAL C 95 LEU C 98 0 SHEET 2 AA9 6 ARG C 129 TRP C 134 -1 O ILE C 133 N LEU C 96 SHEET 3 AA9 6 VAL C 139 GLU C 150 -1 O LEU C 140 N ARG C 132 SHEET 4 AA9 6 GLU C 71 TRP C 82 -1 N GLN C 80 O TYR C 141 SHEET 5 AA9 6 THR C 51 ASN C 66 -1 N GLY C 63 O THR C 73 SHEET 6 AA9 6 ILE C 179 VAL C 190 1 O VAL C 180 N THR C 51 SHEET 1 AB1 4 THR C 111 PHE C 113 0 SHEET 2 AB1 4 GLU C 162 SER C 171 -1 O SER C 170 N PHE C 112 SHEET 3 AB1 4 GLY C 218 ARG C 231 -1 O LEU C 229 N HIS C 163 SHEET 4 AB1 4 PHE C 201 THR C 215 -1 N GLU C 211 O VAL C 222 SHEET 1 AB2 6 VAL D 83 LEU D 86 0 SHEET 2 AB2 6 LYS D 117 THR D 122 -1 O ILE D 121 N LEU D 84 SHEET 3 AB2 6 THR D 126 GLU D 138 -1 O THR D 126 N THR D 122 SHEET 4 AB2 6 GLU D 59 LYS D 71 -1 N ILE D 62 O VAL D 135 SHEET 5 AB2 6 THR D 39 SER D 54 -1 N LYS D 42 O SER D 69 SHEET 6 AB2 6 VAL D 167 TRP D 171 1 O GLU D 170 N THR D 43 SHEET 1 AB3 5 VAL D 108 ALA D 109 0 SHEET 2 AB3 5 THR D 126 GLU D 138 -1 O THR D 134 N VAL D 108 SHEET 3 AB3 5 GLU D 59 LYS D 71 -1 N ILE D 62 O VAL D 135 SHEET 4 AB3 5 THR D 39 SER D 54 -1 N LYS D 42 O SER D 69 SHEET 5 AB3 5 VAL D 179 VAL D 181 1 O VAL D 180 N VAL D 47 SHEET 1 AB4 4 PHE D 100 PHE D 101 0 SHEET 2 AB4 4 LYS D 156 GLY D 158 -1 O GLY D 158 N PHE D 100 SHEET 3 AB4 4 GLU D 209 ARG D 221 -1 O MET D 213 N PHE D 157 SHEET 4 AB4 4 ALA D 150 CYS D 153 -1 N CYS D 153 O PHE D 217 SHEET 1 AB5 4 PHE D 100 PHE D 101 0 SHEET 2 AB5 4 LYS D 156 GLY D 158 -1 O GLY D 158 N PHE D 100 SHEET 3 AB5 4 GLU D 209 ARG D 221 -1 O MET D 213 N PHE D 157 SHEET 4 AB5 4 TYR D 191 GLN D 204 -1 N VAL D 203 O TYR D 210 SHEET 1 AB6 6 THR E 82 LEU E 83 0 SHEET 2 AB6 6 ARG E 114 LEU E 118 -1 O ILE E 116 N LEU E 83 SHEET 3 AB6 6 THR E 123 ALA E 135 -1 O GLY E 127 N MET E 115 SHEET 4 AB6 6 ASP E 56 ARG E 68 -1 N PHE E 63 O LEU E 128 SHEET 5 AB6 6 VAL E 36 SER E 51 -1 N ALA E 45 O THR E 60 SHEET 6 AB6 6 ILE E 164 TRP E 168 1 O GLU E 165 N VAL E 38 SHEET 1 AB7 5 ASP E 101 VAL E 106 0 SHEET 2 AB7 5 THR E 123 ALA E 135 -1 O THR E 131 N PHE E 105 SHEET 3 AB7 5 ASP E 56 ARG E 68 -1 N PHE E 63 O LEU E 128 SHEET 4 AB7 5 VAL E 36 SER E 51 -1 N ALA E 45 O THR E 60 SHEET 5 AB7 5 VAL E 175 THR E 176 1 O THR E 176 N ILE E 42 SHEET 1 AB8 4 THR E 96 TYR E 97 0 SHEET 2 AB8 4 GLU E 147 SER E 156 -1 O GLU E 155 N TYR E 97 SHEET 3 AB8 4 GLY E 203 LYS E 215 -1 O PHE E 212 N CYS E 150 SHEET 4 AB8 4 ILE E 188 PHE E 200 -1 N PHE E 200 O GLY E 203 SHEET 1 AB9 4 GLN F 4 GLY F 9 0 SHEET 2 AB9 4 LEU F 19 SER F 26 -1 O ALA F 24 N GLN F 6 SHEET 3 AB9 4 SER F 78 LEU F 83 -1 O VAL F 79 N CYS F 23 SHEET 4 AB9 4 THR F 69 ASP F 73 -1 N THR F 69 O GLN F 82 SHEET 1 AC1 6 GLY F 11 GLN F 14 0 SHEET 2 AC1 6 THR F 118 SER F 123 1 O SER F 123 N VAL F 13 SHEET 3 AC1 6 ALA F 92 GLY F 99 -1 N ALA F 92 O VAL F 120 SHEET 4 AC1 6 ILE F 34 GLN F 40 -1 N PHE F 38 O TYR F 95 SHEET 5 AC1 6 ARG F 46 MET F 52 -1 O GLU F 47 N ARG F 39 SHEET 6 AC1 6 GLN F 58 TYR F 60 -1 O TYR F 59 N ALA F 51 SHEET 1 AC2 4 GLY F 11 GLN F 14 0 SHEET 2 AC2 4 THR F 118 SER F 123 1 O SER F 123 N VAL F 13 SHEET 3 AC2 4 ALA F 92 GLY F 99 -1 N ALA F 92 O VAL F 120 SHEET 4 AC2 4 TYR F 113 TRP F 114 -1 O TYR F 113 N ALA F 98 SSBOND 1 CYS A 139 CYS A 153 1555 1555 2.03 SSBOND 2 CYS B 136 CYS B 150 1555 1555 2.03 SSBOND 3 CYS C 151 CYS C 165 1555 1555 2.03 SSBOND 4 CYS D 139 CYS D 153 1555 1555 2.03 SSBOND 5 CYS E 136 CYS E 150 1555 1555 2.03 SSBOND 6 CYS F 23 CYS F 96 1555 1555 2.03 LINK ND2 ASN A 111 C1 NAG a 1 1555 1555 1.44 LINK ND2 ASN B 80 C1 NAG b 1 1555 1555 1.44 LINK ND2 ASN B 149 C1 NAG G 1 1555 1555 1.44 LINK ND2 ASN C 208 C1 NAG c 1 1555 1555 1.45 LINK ND2 ASN D 111 C1 NAG d 1 1555 1555 1.44 LINK ND2 ASN E 80 C1 NAG e 1 1555 1555 1.44 LINK ND2 ASN E 149 C1 NAG H 1 1555 1555 1.44 LINK O4 NAG a 1 C1 NAG a 2 1555 1555 1.45 LINK O4 NAG a 2 C1 BMA a 3 1555 1555 1.44 LINK O3 BMA a 3 C1 MAN a 4 1555 1555 1.44 LINK O6 BMA a 3 C1 MAN a 7 1555 1555 1.45 LINK O2 MAN a 4 C1 MAN a 5 1555 1555 1.44 LINK O2 MAN a 5 C1 MAN a 6 1555 1555 1.44 LINK O6 MAN a 7 C1 MAN a 8 1555 1555 1.44 LINK O3 MAN a 7 C1 MAN a 10 1555 1555 1.45 LINK O2 MAN a 8 C1 MAN a 9 1555 1555 1.44 LINK O4 NAG b 1 C1 NAG b 2 1555 1555 1.44 LINK O4 NAG G 1 C1 NAG G 2 1555 1555 1.44 LINK O4 NAG G 2 C1 BMA G 3 1555 1555 1.44 LINK O6 BMA G 3 C1 MAN G 4 1555 1555 1.44 LINK O3 BMA G 3 C1 MAN G 6 1555 1555 1.44 LINK O3 MAN G 4 C1 MAN G 5 1555 1555 1.44 LINK O4 NAG c 1 C1 NAG c 2 1555 1555 1.44 LINK O4 NAG d 1 C1 NAG d 2 1555 1555 1.44 LINK O4 NAG d 2 C1 BMA d 3 1555 1555 1.45 LINK O3 BMA d 3 C1 MAN d 4 1555 1555 1.44 LINK O6 BMA d 3 C1 MAN d 5 1555 1555 1.44 LINK O4 NAG e 1 C1 NAG e 2 1555 1555 1.44 LINK O4 NAG H 1 C1 NAG H 2 1555 1555 1.44 LINK O4 NAG H 2 C1 BMA H 3 1555 1555 1.44 LINK O6 BMA H 3 C1 MAN H 4 1555 1555 1.44 LINK O3 BMA H 3 C1 MAN H 6 1555 1555 1.44 LINK O3 MAN H 4 C1 MAN H 5 1555 1555 1.45 CISPEP 1 MET A 112 THR A 113 0 -1.23 CISPEP 2 PHE A 146 PRO A 147 0 0.90 CISPEP 3 VAL B 109 THR B 110 0 0.75 CISPEP 4 TYR B 143 PRO B 144 0 -2.46 CISPEP 5 ILE C 124 THR C 125 0 -0.84 CISPEP 6 PHE C 158 PRO C 159 0 -2.45 CISPEP 7 MET D 112 THR D 113 0 -2.07 CISPEP 8 PHE D 146 PRO D 147 0 0.24 CISPEP 9 VAL E 109 THR E 110 0 -0.13 CISPEP 10 TYR E 143 PRO E 144 0 -0.20 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000