HEADER ANTIVIRAL PROTEIN 05-JUN-24 9FM1 TITLE CRYO-EM STRUCTURE OF INFLUENZA B/WASHINGTON/02/2019 VIRUS TITLE 2 HEMAGGLUTININ IN COMPLEX WITH SINGLE-DOMAIN ANTIBODY HVHH-69. COMPND MOL_ID: 1; COMPND 2 MOLECULE: HEMAGGLUTININ; COMPND 3 CHAIN: A, B, C; COMPND 4 ENGINEERED: YES; COMPND 5 MOL_ID: 2; COMPND 6 MOLECULE: SINGLE-DOMAIN ANTIBODY HVHH-69; COMPND 7 CHAIN: D, E, F; COMPND 8 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: INFLUENZA B VIRUS; SOURCE 3 ORGANISM_TAXID: 11520; SOURCE 4 GENE: HA; SOURCE 5 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 6 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 7 EXPRESSION_SYSTEM_CELL_LINE: EXPI293F; SOURCE 8 MOL_ID: 2; SOURCE 9 ORGANISM_SCIENTIFIC: LAMA GLAMA; SOURCE 10 ORGANISM_TAXID: 9844; SOURCE 11 EXPRESSION_SYSTEM: KOMAGATAELLA PHAFFII; SOURCE 12 EXPRESSION_SYSTEM_TAXID: 460519 KEYWDS HEMAGGLUTININ, SINGLE-DOMAIN ANTIBODY, COMPLEX, ANTIVIRAL PROTEIN EXPDTA ELECTRON MICROSCOPY AUTHOR J.FELIX,A.MATTHYS,S.N.SAVVIDES,X.SAELENS REVDAT 1 09-JUL-25 9FM1 0 JRNL AUTH A.MATTHYS,J.FELIX,J.P.P.CATANI,K.ROOSE,W.NERINCKX, JRNL AUTH 2 B.VAN BUYTEN,D.FIJALKOWSKA,N.CALLEWAERT,S.N.SAVVIDES, JRNL AUTH 3 X.SAELENS JRNL TITL SINGLE-DOMAIN ANTIBODIES DIRECTED AGAINST HEMAGGLUTININ AND JRNL TITL 2 NEURAMINIDASE PROTECT AGAINST INFLUENZA B VIRUSES JRNL REF NAT COMMUN V. 16 5831 2025 JRNL REFN ESSN 2041-1723 JRNL DOI 10.1038/S41467-025-60232-3 REMARK 2 REMARK 2 RESOLUTION. 2.93 ANGSTROMS. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 SOFTWARE PACKAGES : CRYOSPARC, SERIALEM, CRYOSPARC, PHENIX, REMARK 3 CRYOSPARC, CRYOSPARC, CRYOSPARC, REMARK 3 CRYOSPARC REMARK 3 RECONSTRUCTION SCHEMA : NULL REMARK 3 REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT REMARK 3 PDB ENTRY : NULL REMARK 3 REFINEMENT SPACE : NULL REMARK 3 REFINEMENT PROTOCOL : NULL REMARK 3 REFINEMENT TARGET : NULL REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL REMARK 3 REMARK 3 FITTING PROCEDURE : NULL REMARK 3 REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 2.930 REMARK 3 NUMBER OF PARTICLES : 157829 REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING AND AMPLITUDE REMARK 3 CORRECTION REMARK 3 REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL REMARK 3 REMARK 3 OTHER DETAILS: NULL REMARK 4 REMARK 4 9FM1 COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBE ON 05-JUN-24. REMARK 100 THE DEPOSITION ID IS D_1292138939. REMARK 245 REMARK 245 EXPERIMENTAL DETAILS REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE REMARK 245 SPECIMEN TYPE : NULL REMARK 245 REMARK 245 ELECTRON MICROSCOPE SAMPLE REMARK 245 SAMPLE TYPE : PARTICLE REMARK 245 PARTICLE TYPE : POINT REMARK 245 NAME OF SAMPLE : COMPLEX BETWEEN INFLUENZA REMARK 245 B/WASHINGTON/02/2019 VIRUS REMARK 245 HEMAGGLUTININ AND SINGLE-DOMAIN REMARK 245 ANTIBODY HVHH-69 REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : 0.40 REMARK 245 SAMPLE SUPPORT DETAILS : NULL REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL REMARK 245 SAMPLE BUFFER : NULL REMARK 245 PH : 7.40 REMARK 245 SAMPLE DETAILS : NULL REMARK 245 REMARK 245 DATA ACQUISITION REMARK 245 DATE OF EXPERIMENT : NULL REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : 4352 REMARK 245 TEMPERATURE (KELVIN) : NULL REMARK 245 MICROSCOPE MODEL : JEOL CRYO ARM 300 REMARK 245 DETECTOR TYPE : GATAN K3 (6K X 4K) REMARK 245 MINIMUM DEFOCUS (NM) : 500.00 REMARK 245 MAXIMUM DEFOCUS (NM) : 2500.00 REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 NOMINAL CS : NULL REMARK 245 IMAGING MODE : BRIGHT FIELD REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 6000.00 REMARK 245 ILLUMINATION MODE : FLOOD BEAM REMARK 245 NOMINAL MAGNIFICATION : NULL REMARK 245 CALIBRATED MAGNIFICATION : NULL REMARK 245 SOURCE : FIELD EMISSION GUN REMARK 245 ACCELERATION VOLTAGE (KV) : 300 REMARK 245 IMAGING DETAILS : NULL REMARK 247 REMARK 247 ELECTRON MICROSCOPY REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION REMARK 247 OF THE STRUCTURE FACTORS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: HEXAMERIC REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: HEXAMERIC REMARK 350 SOFTWARE USED: PISA REMARK 350 TOTAL BURIED SURFACE AREA: 14690 ANGSTROM**2 REMARK 350 SURFACE AREA OF THE COMPLEX: 61650 ANGSTROM**2 REMARK 350 CHANGE IN SOLVENT FREE ENERGY: -37.0 KCAL/MOL REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D, E, F REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 REMARK 465 M RES C SSSEQI REMARK 465 MET A -23 REMARK 465 PRO A -22 REMARK 465 MET A -21 REMARK 465 GLY A -20 REMARK 465 SER A -19 REMARK 465 LEU A -18 REMARK 465 GLN A -17 REMARK 465 PRO A -16 REMARK 465 LEU A -15 REMARK 465 ALA A -14 REMARK 465 THR A -13 REMARK 465 LEU A -12 REMARK 465 TYR A -11 REMARK 465 LEU A -10 REMARK 465 LEU A -9 REMARK 465 GLY A -8 REMARK 465 MET A -7 REMARK 465 LEU A -6 REMARK 465 VAL A -5 REMARK 465 ALA A -4 REMARK 465 SER A -3 REMARK 465 VAL A -2 REMARK 465 LEU A -1 REMARK 465 GLY A 0 REMARK 465 ASP A 1 REMARK 465 ARG A 2 REMARK 465 ILE A 3 REMARK 465 CYS A 4 REMARK 465 THR A 5 REMARK 465 GLY A 6 REMARK 465 ILE A 7 REMARK 465 THR A 8 REMARK 465 THR A 332 REMARK 465 LYS A 333 REMARK 465 TYR A 334 REMARK 465 ARG A 335 REMARK 465 PRO A 336 REMARK 465 PRO A 337 REMARK 465 ALA A 338 REMARK 465 LYS A 339 REMARK 465 LEU A 340 REMARK 465 LEU A 341 REMARK 465 LYS A 342 REMARK 465 GLU A 343 REMARK 465 ARG A 344 REMARK 465 GLY A 345 REMARK 465 PHE A 346 REMARK 465 PHE A 347 REMARK 465 GLY A 348 REMARK 465 ALA A 349 REMARK 465 ILE A 350 REMARK 465 ALA A 351 REMARK 465 GLY A 352 REMARK 465 PHE A 353 REMARK 465 LEU A 354 REMARK 465 GLU A 355 REMARK 465 GLY A 356 REMARK 465 GLY A 357 REMARK 465 TRP A 358 REMARK 465 GLU A 359 REMARK 465 GLY A 360 REMARK 465 MET A 361 REMARK 465 ILE A 362 REMARK 465 ALA A 363 REMARK 465 GLY A 364 REMARK 465 TRP A 365 REMARK 465 HIS A 366 REMARK 465 GLY A 367 REMARK 465 TYR A 368 REMARK 465 THR A 369 REMARK 465 SER A 370 REMARK 465 HIS A 371 REMARK 465 GLY A 372 REMARK 465 ALA A 373 REMARK 465 HIS A 374 REMARK 465 GLY A 375 REMARK 465 VAL A 376 REMARK 465 ALA A 377 REMARK 465 VAL A 378 REMARK 465 ALA A 379 REMARK 465 ALA A 380 REMARK 465 ASP A 381 REMARK 465 LEU A 382 REMARK 465 LYS A 383 REMARK 465 SER A 384 REMARK 465 THR A 385 REMARK 465 GLN A 386 REMARK 465 GLU A 387 REMARK 465 ALA A 388 REMARK 465 ILE A 389 REMARK 465 SER A 454 REMARK 465 GLU A 455 REMARK 465 ASP A 456 REMARK 465 GLU A 457 REMARK 465 HIS A 458 REMARK 465 LEU A 459 REMARK 465 LEU A 460 REMARK 465 ALA A 461 REMARK 465 LEU A 462 REMARK 465 GLU A 463 REMARK 465 ARG A 464 REMARK 465 LYS A 465 REMARK 465 LEU A 466 REMARK 465 LYS A 467 REMARK 465 LYS A 468 REMARK 465 MET A 469 REMARK 465 LEU A 470 REMARK 465 GLY A 471 REMARK 465 PRO A 472 REMARK 465 SER A 473 REMARK 465 ALA A 474 REMARK 465 VAL A 475 REMARK 465 GLU A 476 REMARK 465 ILE A 477 REMARK 465 GLY A 478 REMARK 465 ASN A 479 REMARK 465 GLY A 480 REMARK 465 CYS A 481 REMARK 465 PHE A 482 REMARK 465 GLU A 483 REMARK 465 THR A 484 REMARK 465 LYS A 485 REMARK 465 HIS A 486 REMARK 465 LYS A 487 REMARK 465 CYS A 488 REMARK 465 ASN A 489 REMARK 465 GLN A 490 REMARK 465 THR A 491 REMARK 465 CYS A 492 REMARK 465 LEU A 493 REMARK 465 ASP A 494 REMARK 465 ARG A 495 REMARK 465 ILE A 496 REMARK 465 ALA A 497 REMARK 465 ALA A 498 REMARK 465 GLY A 499 REMARK 465 THR A 500 REMARK 465 PHE A 501 REMARK 465 ASP A 502 REMARK 465 ALA A 503 REMARK 465 GLY A 504 REMARK 465 GLU A 505 REMARK 465 PHE A 506 REMARK 465 SER A 507 REMARK 465 LEU A 508 REMARK 465 PRO A 509 REMARK 465 THR A 510 REMARK 465 PHE A 511 REMARK 465 ASP A 512 REMARK 465 SER A 513 REMARK 465 LEU A 514 REMARK 465 ASN A 515 REMARK 465 ILE A 516 REMARK 465 THR A 517 REMARK 465 ALA A 518 REMARK 465 ALA A 519 REMARK 465 SER A 520 REMARK 465 LEU A 521 REMARK 465 ASN A 522 REMARK 465 ASP A 523 REMARK 465 ASP A 524 REMARK 465 GLY A 525 REMARK 465 LEU A 526 REMARK 465 ASP A 527 REMARK 465 ASN A 528 REMARK 465 HIS A 529 REMARK 465 THR A 530 REMARK 465 ILE A 531 REMARK 465 SER A 532 REMARK 465 GLY A 533 REMARK 465 LYS A 534 REMARK 465 ARG A 535 REMARK 465 MET A 536 REMARK 465 LYS A 537 REMARK 465 GLN A 538 REMARK 465 ILE A 539 REMARK 465 GLU A 540 REMARK 465 ASP A 541 REMARK 465 LYS A 542 REMARK 465 ILE A 543 REMARK 465 GLU A 544 REMARK 465 GLU A 545 REMARK 465 ILE A 546 REMARK 465 GLU A 547 REMARK 465 SER A 548 REMARK 465 LYS A 549 REMARK 465 GLN A 550 REMARK 465 LYS A 551 REMARK 465 LYS A 552 REMARK 465 ILE A 553 REMARK 465 GLU A 554 REMARK 465 ASN A 555 REMARK 465 GLU A 556 REMARK 465 ILE A 557 REMARK 465 ALA A 558 REMARK 465 ARG A 559 REMARK 465 ILE A 560 REMARK 465 LYS A 561 REMARK 465 LYS A 562 REMARK 465 LEU A 563 REMARK 465 ILE A 564 REMARK 465 GLY A 565 REMARK 465 GLU A 566 REMARK 465 ARG A 567 REMARK 465 SER A 568 REMARK 465 ALA A 569 REMARK 465 TRP A 570 REMARK 465 SER A 571 REMARK 465 HIS A 572 REMARK 465 PRO A 573 REMARK 465 GLN A 574 REMARK 465 PHE A 575 REMARK 465 GLU A 576 REMARK 465 LYS A 577 REMARK 465 MET B -23 REMARK 465 PRO B -22 REMARK 465 MET B -21 REMARK 465 GLY B -20 REMARK 465 SER B -19 REMARK 465 LEU B -18 REMARK 465 GLN B -17 REMARK 465 PRO B -16 REMARK 465 LEU B -15 REMARK 465 ALA B -14 REMARK 465 THR B -13 REMARK 465 LEU B -12 REMARK 465 TYR B -11 REMARK 465 LEU B -10 REMARK 465 LEU B -9 REMARK 465 GLY B -8 REMARK 465 MET B -7 REMARK 465 LEU B -6 REMARK 465 VAL B -5 REMARK 465 ALA B -4 REMARK 465 SER B -3 REMARK 465 VAL B -2 REMARK 465 LEU B -1 REMARK 465 GLY B 0 REMARK 465 ASP B 1 REMARK 465 ARG B 2 REMARK 465 ILE B 3 REMARK 465 CYS B 4 REMARK 465 THR B 5 REMARK 465 GLY B 6 REMARK 465 ILE B 7 REMARK 465 THR B 8 REMARK 465 THR B 332 REMARK 465 LYS B 333 REMARK 465 TYR B 334 REMARK 465 ARG B 335 REMARK 465 PRO B 336 REMARK 465 PRO B 337 REMARK 465 ALA B 338 REMARK 465 LYS B 339 REMARK 465 LEU B 340 REMARK 465 LEU B 341 REMARK 465 LYS B 342 REMARK 465 GLU B 343 REMARK 465 ARG B 344 REMARK 465 GLY B 345 REMARK 465 PHE B 346 REMARK 465 PHE B 347 REMARK 465 GLY B 348 REMARK 465 ALA B 349 REMARK 465 ILE B 350 REMARK 465 ALA B 351 REMARK 465 GLY B 352 REMARK 465 PHE B 353 REMARK 465 LEU B 354 REMARK 465 GLU B 355 REMARK 465 GLY B 356 REMARK 465 GLY B 357 REMARK 465 TRP B 358 REMARK 465 GLU B 359 REMARK 465 GLY B 360 REMARK 465 MET B 361 REMARK 465 ILE B 362 REMARK 465 ALA B 363 REMARK 465 GLY B 364 REMARK 465 TRP B 365 REMARK 465 HIS B 366 REMARK 465 GLY B 367 REMARK 465 TYR B 368 REMARK 465 THR B 369 REMARK 465 SER B 370 REMARK 465 HIS B 371 REMARK 465 GLY B 372 REMARK 465 ALA B 373 REMARK 465 HIS B 374 REMARK 465 GLY B 375 REMARK 465 VAL B 376 REMARK 465 ALA B 377 REMARK 465 VAL B 378 REMARK 465 ALA B 379 REMARK 465 ALA B 380 REMARK 465 ASP B 381 REMARK 465 LEU B 382 REMARK 465 LYS B 383 REMARK 465 SER B 384 REMARK 465 THR B 385 REMARK 465 GLN B 386 REMARK 465 GLU B 387 REMARK 465 ALA B 388 REMARK 465 ILE B 389 REMARK 465 SER B 454 REMARK 465 GLU B 455 REMARK 465 ASP B 456 REMARK 465 GLU B 457 REMARK 465 HIS B 458 REMARK 465 LEU B 459 REMARK 465 LEU B 460 REMARK 465 ALA B 461 REMARK 465 LEU B 462 REMARK 465 GLU B 463 REMARK 465 ARG B 464 REMARK 465 LYS B 465 REMARK 465 LEU B 466 REMARK 465 LYS B 467 REMARK 465 LYS B 468 REMARK 465 MET B 469 REMARK 465 LEU B 470 REMARK 465 GLY B 471 REMARK 465 PRO B 472 REMARK 465 SER B 473 REMARK 465 ALA B 474 REMARK 465 VAL B 475 REMARK 465 GLU B 476 REMARK 465 ILE B 477 REMARK 465 GLY B 478 REMARK 465 ASN B 479 REMARK 465 GLY B 480 REMARK 465 CYS B 481 REMARK 465 PHE B 482 REMARK 465 GLU B 483 REMARK 465 THR B 484 REMARK 465 LYS B 485 REMARK 465 HIS B 486 REMARK 465 LYS B 487 REMARK 465 CYS B 488 REMARK 465 ASN B 489 REMARK 465 GLN B 490 REMARK 465 THR B 491 REMARK 465 CYS B 492 REMARK 465 LEU B 493 REMARK 465 ASP B 494 REMARK 465 ARG B 495 REMARK 465 ILE B 496 REMARK 465 ALA B 497 REMARK 465 ALA B 498 REMARK 465 GLY B 499 REMARK 465 THR B 500 REMARK 465 PHE B 501 REMARK 465 ASP B 502 REMARK 465 ALA B 503 REMARK 465 GLY B 504 REMARK 465 GLU B 505 REMARK 465 PHE B 506 REMARK 465 SER B 507 REMARK 465 LEU B 508 REMARK 465 PRO B 509 REMARK 465 THR B 510 REMARK 465 PHE B 511 REMARK 465 ASP B 512 REMARK 465 SER B 513 REMARK 465 LEU B 514 REMARK 465 ASN B 515 REMARK 465 ILE B 516 REMARK 465 THR B 517 REMARK 465 ALA B 518 REMARK 465 ALA B 519 REMARK 465 SER B 520 REMARK 465 LEU B 521 REMARK 465 ASN B 522 REMARK 465 ASP B 523 REMARK 465 ASP B 524 REMARK 465 GLY B 525 REMARK 465 LEU B 526 REMARK 465 ASP B 527 REMARK 465 ASN B 528 REMARK 465 HIS B 529 REMARK 465 THR B 530 REMARK 465 ILE B 531 REMARK 465 SER B 532 REMARK 465 GLY B 533 REMARK 465 LYS B 534 REMARK 465 ARG B 535 REMARK 465 MET B 536 REMARK 465 LYS B 537 REMARK 465 GLN B 538 REMARK 465 ILE B 539 REMARK 465 GLU B 540 REMARK 465 ASP B 541 REMARK 465 LYS B 542 REMARK 465 ILE B 543 REMARK 465 GLU B 544 REMARK 465 GLU B 545 REMARK 465 ILE B 546 REMARK 465 GLU B 547 REMARK 465 SER B 548 REMARK 465 LYS B 549 REMARK 465 GLN B 550 REMARK 465 LYS B 551 REMARK 465 LYS B 552 REMARK 465 ILE B 553 REMARK 465 GLU B 554 REMARK 465 ASN B 555 REMARK 465 GLU B 556 REMARK 465 ILE B 557 REMARK 465 ALA B 558 REMARK 465 ARG B 559 REMARK 465 ILE B 560 REMARK 465 LYS B 561 REMARK 465 LYS B 562 REMARK 465 LEU B 563 REMARK 465 ILE B 564 REMARK 465 GLY B 565 REMARK 465 GLU B 566 REMARK 465 ARG B 567 REMARK 465 SER B 568 REMARK 465 ALA B 569 REMARK 465 TRP B 570 REMARK 465 SER B 571 REMARK 465 HIS B 572 REMARK 465 PRO B 573 REMARK 465 GLN B 574 REMARK 465 PHE B 575 REMARK 465 GLU B 576 REMARK 465 LYS B 577 REMARK 465 MET C -23 REMARK 465 PRO C -22 REMARK 465 MET C -21 REMARK 465 GLY C -20 REMARK 465 SER C -19 REMARK 465 LEU C -18 REMARK 465 GLN C -17 REMARK 465 PRO C -16 REMARK 465 LEU C -15 REMARK 465 ALA C -14 REMARK 465 THR C -13 REMARK 465 LEU C -12 REMARK 465 TYR C -11 REMARK 465 LEU C -10 REMARK 465 LEU C -9 REMARK 465 GLY C -8 REMARK 465 MET C -7 REMARK 465 LEU C -6 REMARK 465 VAL C -5 REMARK 465 ALA C -4 REMARK 465 SER C -3 REMARK 465 VAL C -2 REMARK 465 LEU C -1 REMARK 465 GLY C 0 REMARK 465 ASP C 1 REMARK 465 ARG C 2 REMARK 465 ILE C 3 REMARK 465 CYS C 4 REMARK 465 THR C 5 REMARK 465 GLY C 6 REMARK 465 ILE C 7 REMARK 465 THR C 8 REMARK 465 THR C 332 REMARK 465 LYS C 333 REMARK 465 TYR C 334 REMARK 465 ARG C 335 REMARK 465 PRO C 336 REMARK 465 PRO C 337 REMARK 465 ALA C 338 REMARK 465 LYS C 339 REMARK 465 LEU C 340 REMARK 465 LEU C 341 REMARK 465 LYS C 342 REMARK 465 GLU C 343 REMARK 465 ARG C 344 REMARK 465 GLY C 345 REMARK 465 PHE C 346 REMARK 465 PHE C 347 REMARK 465 GLY C 348 REMARK 465 ALA C 349 REMARK 465 ILE C 350 REMARK 465 ALA C 351 REMARK 465 GLY C 352 REMARK 465 PHE C 353 REMARK 465 LEU C 354 REMARK 465 GLU C 355 REMARK 465 GLY C 356 REMARK 465 GLY C 357 REMARK 465 TRP C 358 REMARK 465 GLU C 359 REMARK 465 GLY C 360 REMARK 465 MET C 361 REMARK 465 ILE C 362 REMARK 465 ALA C 363 REMARK 465 GLY C 364 REMARK 465 TRP C 365 REMARK 465 HIS C 366 REMARK 465 GLY C 367 REMARK 465 TYR C 368 REMARK 465 THR C 369 REMARK 465 SER C 370 REMARK 465 HIS C 371 REMARK 465 GLY C 372 REMARK 465 ALA C 373 REMARK 465 HIS C 374 REMARK 465 GLY C 375 REMARK 465 VAL C 376 REMARK 465 ALA C 377 REMARK 465 VAL C 378 REMARK 465 ALA C 379 REMARK 465 ALA C 380 REMARK 465 ASP C 381 REMARK 465 LEU C 382 REMARK 465 LYS C 383 REMARK 465 SER C 384 REMARK 465 THR C 385 REMARK 465 GLN C 386 REMARK 465 GLU C 387 REMARK 465 ALA C 388 REMARK 465 ILE C 389 REMARK 465 SER C 454 REMARK 465 GLU C 455 REMARK 465 ASP C 456 REMARK 465 GLU C 457 REMARK 465 HIS C 458 REMARK 465 LEU C 459 REMARK 465 LEU C 460 REMARK 465 ALA C 461 REMARK 465 LEU C 462 REMARK 465 GLU C 463 REMARK 465 ARG C 464 REMARK 465 LYS C 465 REMARK 465 LEU C 466 REMARK 465 LYS C 467 REMARK 465 LYS C 468 REMARK 465 MET C 469 REMARK 465 LEU C 470 REMARK 465 GLY C 471 REMARK 465 PRO C 472 REMARK 465 SER C 473 REMARK 465 ALA C 474 REMARK 465 VAL C 475 REMARK 465 GLU C 476 REMARK 465 ILE C 477 REMARK 465 GLY C 478 REMARK 465 ASN C 479 REMARK 465 GLY C 480 REMARK 465 CYS C 481 REMARK 465 PHE C 482 REMARK 465 GLU C 483 REMARK 465 THR C 484 REMARK 465 LYS C 485 REMARK 465 HIS C 486 REMARK 465 LYS C 487 REMARK 465 CYS C 488 REMARK 465 ASN C 489 REMARK 465 GLN C 490 REMARK 465 THR C 491 REMARK 465 CYS C 492 REMARK 465 LEU C 493 REMARK 465 ASP C 494 REMARK 465 ARG C 495 REMARK 465 ILE C 496 REMARK 465 ALA C 497 REMARK 465 ALA C 498 REMARK 465 GLY C 499 REMARK 465 THR C 500 REMARK 465 PHE C 501 REMARK 465 ASP C 502 REMARK 465 ALA C 503 REMARK 465 GLY C 504 REMARK 465 GLU C 505 REMARK 465 PHE C 506 REMARK 465 SER C 507 REMARK 465 LEU C 508 REMARK 465 PRO C 509 REMARK 465 THR C 510 REMARK 465 PHE C 511 REMARK 465 ASP C 512 REMARK 465 SER C 513 REMARK 465 LEU C 514 REMARK 465 ASN C 515 REMARK 465 ILE C 516 REMARK 465 THR C 517 REMARK 465 ALA C 518 REMARK 465 ALA C 519 REMARK 465 SER C 520 REMARK 465 LEU C 521 REMARK 465 ASN C 522 REMARK 465 ASP C 523 REMARK 465 ASP C 524 REMARK 465 GLY C 525 REMARK 465 LEU C 526 REMARK 465 ASP C 527 REMARK 465 ASN C 528 REMARK 465 HIS C 529 REMARK 465 THR C 530 REMARK 465 ILE C 531 REMARK 465 SER C 532 REMARK 465 GLY C 533 REMARK 465 LYS C 534 REMARK 465 ARG C 535 REMARK 465 MET C 536 REMARK 465 LYS C 537 REMARK 465 GLN C 538 REMARK 465 ILE C 539 REMARK 465 GLU C 540 REMARK 465 ASP C 541 REMARK 465 LYS C 542 REMARK 465 ILE C 543 REMARK 465 GLU C 544 REMARK 465 GLU C 545 REMARK 465 ILE C 546 REMARK 465 GLU C 547 REMARK 465 SER C 548 REMARK 465 LYS C 549 REMARK 465 GLN C 550 REMARK 465 LYS C 551 REMARK 465 LYS C 552 REMARK 465 ILE C 553 REMARK 465 GLU C 554 REMARK 465 ASN C 555 REMARK 465 GLU C 556 REMARK 465 ILE C 557 REMARK 465 ALA C 558 REMARK 465 ARG C 559 REMARK 465 ILE C 560 REMARK 465 LYS C 561 REMARK 465 LYS C 562 REMARK 465 LEU C 563 REMARK 465 ILE C 564 REMARK 465 GLY C 565 REMARK 465 GLU C 566 REMARK 465 ARG C 567 REMARK 465 SER C 568 REMARK 465 ALA C 569 REMARK 465 TRP C 570 REMARK 465 SER C 571 REMARK 465 HIS C 572 REMARK 465 PRO C 573 REMARK 465 GLN C 574 REMARK 465 PHE C 575 REMARK 465 GLU C 576 REMARK 465 LYS C 577 REMARK 470 REMARK 470 MISSING ATOM REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS (M=MODEL NUMBER; REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER; REMARK 470 I=INSERTION CODE): REMARK 470 M RES CSSEQI ATOMS REMARK 470 ARG A 88 CG CD NE CZ NH1 NH2 REMARK 470 GLU A 128 CG CD OE1 OE2 REMARK 470 ASP A 129 CG OD1 OD2 REMARK 470 LYS A 162 CG CD CE NZ REMARK 470 GLU A 180 CG CD OE1 OE2 REMARK 470 LYS A 200 CG CD CE NZ REMARK 470 LYS A 206 CG CD CE NZ REMARK 470 LYS A 254 CG CD CE NZ REMARK 470 ARG A 276 CG CD NE CZ NH1 NH2 REMARK 470 LYS A 296 CG CD CE NZ REMARK 470 LYS A 391 CG CD CE NZ REMARK 470 LYS A 394 CG CD CE NZ REMARK 470 ARG B 88 CG CD NE CZ NH1 NH2 REMARK 470 GLU B 128 CG CD OE1 OE2 REMARK 470 ASP B 129 CG OD1 OD2 REMARK 470 LYS B 162 CG CD CE NZ REMARK 470 GLU B 180 CG CD OE1 OE2 REMARK 470 LYS B 200 CG CD CE NZ REMARK 470 LYS B 206 CG CD CE NZ REMARK 470 LYS B 254 CG CD CE NZ REMARK 470 ARG B 276 CG CD NE CZ NH1 NH2 REMARK 470 LYS B 296 CG CD CE NZ REMARK 470 LYS B 391 CG CD CE NZ REMARK 470 LYS B 394 CG CD CE NZ REMARK 470 ARG C 88 CG CD NE CZ NH1 NH2 REMARK 470 GLU C 128 CG CD OE1 OE2 REMARK 470 ASP C 129 CG OD1 OD2 REMARK 470 LYS C 162 CG CD CE NZ REMARK 470 GLU C 180 CG CD OE1 OE2 REMARK 470 LYS C 200 CG CD CE NZ REMARK 470 LYS C 206 CG CD CE NZ REMARK 470 LYS C 254 CG CD CE NZ REMARK 470 ARG C 276 CG CD NE CZ NH1 NH2 REMARK 470 LYS C 296 CG CD CE NZ REMARK 470 LYS C 391 CG CD CE NZ REMARK 470 LYS C 394 CG CD CE NZ REMARK 470 ARG D 53 CG CD NE CZ NH1 NH2 REMARK 470 LYS D 75 CG CD CE NZ REMARK 470 LYS D 100F CG CD CE NZ REMARK 470 ARG E 53 CG CD NE CZ NH1 NH2 REMARK 470 LYS E 75 CG CD CE NZ REMARK 470 LYS E 100F CG CD CE NZ REMARK 470 ARG F 53 CG CD NE CZ NH1 NH2 REMARK 470 LYS F 75 CG CD CE NZ REMARK 470 LYS F 100F CG CD CE NZ REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT REMARK 500 REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT. REMARK 500 REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE REMARK 500 OH TYR A 176 O GLU A 182 2.19 REMARK 500 REMARK 500 REMARK: NULL REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 THR A 27 57.58 -92.40 REMARK 500 CYS A 94 -158.25 -151.82 REMARK 500 LYS A 251 -167.89 -79.89 REMARK 500 PRO A 325 96.27 -62.10 REMARK 500 SER A 411 -2.95 67.14 REMARK 500 CYS B 94 -158.75 -150.50 REMARK 500 LYS B 251 -167.02 -79.60 REMARK 500 PRO B 325 95.42 -61.71 REMARK 500 SER B 411 -0.52 66.04 REMARK 500 THR C 27 57.41 -93.81 REMARK 500 CYS C 94 -158.65 -151.97 REMARK 500 SER C 411 -0.79 65.54 REMARK 500 ASP D 29 3.14 -69.03 REMARK 500 ARG E 66 -50.11 -124.94 REMARK 500 SER E 99 -168.97 -118.16 REMARK 500 ASP F 29 1.24 -68.38 REMARK 500 SER F 99 -166.55 -125.48 REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: EMD-50546 RELATED DB: EMDB REMARK 900 CRYO-EM STRUCTURE OF INFLUENZA B/WASHINGTON/02/2019 VIRUS REMARK 900 HEMAGGLUTININ IN COMPLEX WITH SINGLE-DOMAIN ANTIBODY HVHH-69. DBREF1 9FM1 A 1 531 UNP A0A6B9RSJ6_9INFB DBREF2 9FM1 A A0A6B9RSJ6 16 546 DBREF1 9FM1 B 1 531 UNP A0A6B9RSJ6_9INFB DBREF2 9FM1 B A0A6B9RSJ6 16 546 DBREF1 9FM1 C 1 531 UNP A0A6B9RSJ6_9INFB DBREF2 9FM1 C A0A6B9RSJ6 16 546 DBREF 9FM1 D 1 113 PDB 9FM1 9FM1 1 113 DBREF 9FM1 E 1 113 PDB 9FM1 9FM1 1 113 DBREF 9FM1 F 1 113 PDB 9FM1 9FM1 1 113 SEQADV 9FM1 MET A -23 UNP A0A6B9RSJ INITIATING METHIONINE SEQADV 9FM1 PRO A -22 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET A -21 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY A -20 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER A -19 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU A -18 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN A -17 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PRO A -16 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU A -15 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA A -14 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 THR A -13 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU A -12 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 TYR A -11 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU A -10 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU A -9 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY A -8 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET A -7 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU A -6 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 VAL A -5 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA A -4 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER A -3 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 VAL A -2 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU A -1 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY A 0 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER A 532 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY A 533 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 534 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG A 535 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET A 536 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 537 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN A 538 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE A 539 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU A 540 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ASP A 541 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 542 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE A 543 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU A 544 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU A 545 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE A 546 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU A 547 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER A 548 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 549 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN A 550 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 551 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 552 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE A 553 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU A 554 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ASN A 555 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU A 556 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE A 557 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA A 558 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG A 559 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE A 560 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 561 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 562 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU A 563 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE A 564 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY A 565 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU A 566 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG A 567 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER A 568 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA A 569 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 TRP A 570 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER A 571 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 HIS A 572 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PRO A 573 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN A 574 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PHE A 575 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU A 576 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS A 577 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET B -23 UNP A0A6B9RSJ INITIATING METHIONINE SEQADV 9FM1 PRO B -22 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET B -21 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY B -20 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER B -19 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU B -18 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN B -17 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PRO B -16 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU B -15 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA B -14 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 THR B -13 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU B -12 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 TYR B -11 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU B -10 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU B -9 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY B -8 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET B -7 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU B -6 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 VAL B -5 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA B -4 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER B -3 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 VAL B -2 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU B -1 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY B 0 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER B 532 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY B 533 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 534 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG B 535 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET B 536 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 537 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN B 538 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE B 539 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU B 540 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ASP B 541 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 542 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE B 543 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU B 544 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU B 545 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE B 546 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU B 547 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER B 548 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 549 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN B 550 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 551 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 552 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE B 553 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU B 554 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ASN B 555 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU B 556 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE B 557 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA B 558 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG B 559 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE B 560 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 561 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 562 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU B 563 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE B 564 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY B 565 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU B 566 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG B 567 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER B 568 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA B 569 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 TRP B 570 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER B 571 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 HIS B 572 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PRO B 573 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN B 574 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PHE B 575 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU B 576 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS B 577 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET C -23 UNP A0A6B9RSJ INITIATING METHIONINE SEQADV 9FM1 PRO C -22 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET C -21 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY C -20 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER C -19 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU C -18 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN C -17 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PRO C -16 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU C -15 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA C -14 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 THR C -13 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU C -12 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 TYR C -11 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU C -10 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU C -9 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY C -8 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET C -7 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU C -6 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 VAL C -5 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA C -4 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER C -3 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 VAL C -2 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU C -1 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY C 0 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER C 532 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY C 533 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 534 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG C 535 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 MET C 536 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 537 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN C 538 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE C 539 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU C 540 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ASP C 541 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 542 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE C 543 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU C 544 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU C 545 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE C 546 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU C 547 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER C 548 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 549 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN C 550 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 551 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 552 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE C 553 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU C 554 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ASN C 555 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU C 556 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE C 557 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA C 558 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG C 559 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE C 560 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 561 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 562 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LEU C 563 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ILE C 564 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLY C 565 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU C 566 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ARG C 567 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER C 568 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 ALA C 569 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 TRP C 570 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 SER C 571 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 HIS C 572 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PRO C 573 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLN C 574 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 PHE C 575 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 GLU C 576 UNP A0A6B9RSJ EXPRESSION TAG SEQADV 9FM1 LYS C 577 UNP A0A6B9RSJ EXPRESSION TAG SEQRES 1 A 601 MET PRO MET GLY SER LEU GLN PRO LEU ALA THR LEU TYR SEQRES 2 A 601 LEU LEU GLY MET LEU VAL ALA SER VAL LEU GLY ASP ARG SEQRES 3 A 601 ILE CYS THR GLY ILE THR SER SER ASN SER PRO HIS VAL SEQRES 4 A 601 VAL LYS THR ALA THR GLN GLY GLU VAL ASN VAL THR GLY SEQRES 5 A 601 VAL ILE PRO LEU THR THR THR PRO THR LYS SER HIS PHE SEQRES 6 A 601 ALA ASN LEU LYS GLY THR GLU THR ARG GLY LYS LEU CYS SEQRES 7 A 601 PRO LYS CYS LEU ASN CYS THR ASP LEU ASP VAL ALA LEU SEQRES 8 A 601 GLY ARG PRO LYS CYS THR GLY LYS ILE PRO SER ALA ARG SEQRES 9 A 601 VAL SER ILE LEU HIS GLU VAL ARG PRO VAL THR SER GLY SEQRES 10 A 601 CYS PHE PRO ILE MET HIS ASP ARG THR LYS ILE ARG GLN SEQRES 11 A 601 LEU PRO ASN LEU LEU ARG GLY TYR GLU HIS VAL ARG LEU SEQRES 12 A 601 SER THR HIS ASN VAL ILE ASN ALA GLU ASP ALA PRO GLY SEQRES 13 A 601 ARG PRO TYR GLU ILE GLY THR SER GLY SER CYS PRO ASN SEQRES 14 A 601 ILE THR ASN GLY ASN GLY PHE PHE ALA THR MET ALA TRP SEQRES 15 A 601 ALA VAL PRO LYS ASN LYS THR ALA THR ASN PRO LEU THR SEQRES 16 A 601 ILE GLU VAL PRO TYR ILE CYS THR GLU GLY GLU ASP GLN SEQRES 17 A 601 ILE THR VAL TRP GLY PHE HIS SER ASP ASN GLU THR GLN SEQRES 18 A 601 MET ALA LYS LEU TYR GLY ASP SER LYS PRO GLN LYS PHE SEQRES 19 A 601 THR SER SER ALA ASN GLY VAL THR THR HIS TYR VAL SER SEQRES 20 A 601 GLN ILE GLY GLY PHE PRO ASN GLN THR GLU ASP GLY GLY SEQRES 21 A 601 LEU PRO GLN SER GLY ARG ILE VAL VAL ASP TYR MET VAL SEQRES 22 A 601 GLN LYS SER GLY LYS THR GLY THR ILE THR TYR GLN ARG SEQRES 23 A 601 GLY ILE LEU LEU PRO GLN LYS VAL TRP CYS ALA SER GLY SEQRES 24 A 601 ARG SER LYS VAL ILE LYS GLY SER LEU PRO LEU ILE GLY SEQRES 25 A 601 GLU ALA ASP CYS LEU HIS GLU LYS TYR GLY GLY LEU ASN SEQRES 26 A 601 LYS SER LYS PRO TYR TYR THR GLY GLU HIS ALA LYS ALA SEQRES 27 A 601 ILE GLY ASN CYS PRO ILE TRP VAL LYS THR PRO LEU LYS SEQRES 28 A 601 LEU ALA ASN GLY THR LYS TYR ARG PRO PRO ALA LYS LEU SEQRES 29 A 601 LEU LYS GLU ARG GLY PHE PHE GLY ALA ILE ALA GLY PHE SEQRES 30 A 601 LEU GLU GLY GLY TRP GLU GLY MET ILE ALA GLY TRP HIS SEQRES 31 A 601 GLY TYR THR SER HIS GLY ALA HIS GLY VAL ALA VAL ALA SEQRES 32 A 601 ALA ASP LEU LYS SER THR GLN GLU ALA ILE ASN LYS ILE SEQRES 33 A 601 THR LYS ASN LEU ASN SER LEU SER GLU LEU GLU VAL LYS SEQRES 34 A 601 ASN LEU GLN ARG LEU SER GLY ALA MET ASP GLU LEU HIS SEQRES 35 A 601 ASN GLU ILE LEU GLU LEU ASP GLU LYS VAL ASP ASP LEU SEQRES 36 A 601 ARG ALA ASP THR ILE SER SER GLN ILE GLU LEU ALA VAL SEQRES 37 A 601 LEU LEU SER ASN GLU GLY ILE ILE ASN SER GLU ASP GLU SEQRES 38 A 601 HIS LEU LEU ALA LEU GLU ARG LYS LEU LYS LYS MET LEU SEQRES 39 A 601 GLY PRO SER ALA VAL GLU ILE GLY ASN GLY CYS PHE GLU SEQRES 40 A 601 THR LYS HIS LYS CYS ASN GLN THR CYS LEU ASP ARG ILE SEQRES 41 A 601 ALA ALA GLY THR PHE ASP ALA GLY GLU PHE SER LEU PRO SEQRES 42 A 601 THR PHE ASP SER LEU ASN ILE THR ALA ALA SER LEU ASN SEQRES 43 A 601 ASP ASP GLY LEU ASP ASN HIS THR ILE SER GLY LYS ARG SEQRES 44 A 601 MET LYS GLN ILE GLU ASP LYS ILE GLU GLU ILE GLU SER SEQRES 45 A 601 LYS GLN LYS LYS ILE GLU ASN GLU ILE ALA ARG ILE LYS SEQRES 46 A 601 LYS LEU ILE GLY GLU ARG SER ALA TRP SER HIS PRO GLN SEQRES 47 A 601 PHE GLU LYS SEQRES 1 B 601 MET PRO MET GLY SER LEU GLN PRO LEU ALA THR LEU TYR SEQRES 2 B 601 LEU LEU GLY MET LEU VAL ALA SER VAL LEU GLY ASP ARG SEQRES 3 B 601 ILE CYS THR GLY ILE THR SER SER ASN SER PRO HIS VAL SEQRES 4 B 601 VAL LYS THR ALA THR GLN GLY GLU VAL ASN VAL THR GLY SEQRES 5 B 601 VAL ILE PRO LEU THR THR THR PRO THR LYS SER HIS PHE SEQRES 6 B 601 ALA ASN LEU LYS GLY THR GLU THR ARG GLY LYS LEU CYS SEQRES 7 B 601 PRO LYS CYS LEU ASN CYS THR ASP LEU ASP VAL ALA LEU SEQRES 8 B 601 GLY ARG PRO LYS CYS THR GLY LYS ILE PRO SER ALA ARG SEQRES 9 B 601 VAL SER ILE LEU HIS GLU VAL ARG PRO VAL THR SER GLY SEQRES 10 B 601 CYS PHE PRO ILE MET HIS ASP ARG THR LYS ILE ARG GLN SEQRES 11 B 601 LEU PRO ASN LEU LEU ARG GLY TYR GLU HIS VAL ARG LEU SEQRES 12 B 601 SER THR HIS ASN VAL ILE ASN ALA GLU ASP ALA PRO GLY SEQRES 13 B 601 ARG PRO TYR GLU ILE GLY THR SER GLY SER CYS PRO ASN SEQRES 14 B 601 ILE THR ASN GLY ASN GLY PHE PHE ALA THR MET ALA TRP SEQRES 15 B 601 ALA VAL PRO LYS ASN LYS THR ALA THR ASN PRO LEU THR SEQRES 16 B 601 ILE GLU VAL PRO TYR ILE CYS THR GLU GLY GLU ASP GLN SEQRES 17 B 601 ILE THR VAL TRP GLY PHE HIS SER ASP ASN GLU THR GLN SEQRES 18 B 601 MET ALA LYS LEU TYR GLY ASP SER LYS PRO GLN LYS PHE SEQRES 19 B 601 THR SER SER ALA ASN GLY VAL THR THR HIS TYR VAL SER SEQRES 20 B 601 GLN ILE GLY GLY PHE PRO ASN GLN THR GLU ASP GLY GLY SEQRES 21 B 601 LEU PRO GLN SER GLY ARG ILE VAL VAL ASP TYR MET VAL SEQRES 22 B 601 GLN LYS SER GLY LYS THR GLY THR ILE THR TYR GLN ARG SEQRES 23 B 601 GLY ILE LEU LEU PRO GLN LYS VAL TRP CYS ALA SER GLY SEQRES 24 B 601 ARG SER LYS VAL ILE LYS GLY SER LEU PRO LEU ILE GLY SEQRES 25 B 601 GLU ALA ASP CYS LEU HIS GLU LYS TYR GLY GLY LEU ASN SEQRES 26 B 601 LYS SER LYS PRO TYR TYR THR GLY GLU HIS ALA LYS ALA SEQRES 27 B 601 ILE GLY ASN CYS PRO ILE TRP VAL LYS THR PRO LEU LYS SEQRES 28 B 601 LEU ALA ASN GLY THR LYS TYR ARG PRO PRO ALA LYS LEU SEQRES 29 B 601 LEU LYS GLU ARG GLY PHE PHE GLY ALA ILE ALA GLY PHE SEQRES 30 B 601 LEU GLU GLY GLY TRP GLU GLY MET ILE ALA GLY TRP HIS SEQRES 31 B 601 GLY TYR THR SER HIS GLY ALA HIS GLY VAL ALA VAL ALA SEQRES 32 B 601 ALA ASP LEU LYS SER THR GLN GLU ALA ILE ASN LYS ILE SEQRES 33 B 601 THR LYS ASN LEU ASN SER LEU SER GLU LEU GLU VAL LYS SEQRES 34 B 601 ASN LEU GLN ARG LEU SER GLY ALA MET ASP GLU LEU HIS SEQRES 35 B 601 ASN GLU ILE LEU GLU LEU ASP GLU LYS VAL ASP ASP LEU SEQRES 36 B 601 ARG ALA ASP THR ILE SER SER GLN ILE GLU LEU ALA VAL SEQRES 37 B 601 LEU LEU SER ASN GLU GLY ILE ILE ASN SER GLU ASP GLU SEQRES 38 B 601 HIS LEU LEU ALA LEU GLU ARG LYS LEU LYS LYS MET LEU SEQRES 39 B 601 GLY PRO SER ALA VAL GLU ILE GLY ASN GLY CYS PHE GLU SEQRES 40 B 601 THR LYS HIS LYS CYS ASN GLN THR CYS LEU ASP ARG ILE SEQRES 41 B 601 ALA ALA GLY THR PHE ASP ALA GLY GLU PHE SER LEU PRO SEQRES 42 B 601 THR PHE ASP SER LEU ASN ILE THR ALA ALA SER LEU ASN SEQRES 43 B 601 ASP ASP GLY LEU ASP ASN HIS THR ILE SER GLY LYS ARG SEQRES 44 B 601 MET LYS GLN ILE GLU ASP LYS ILE GLU GLU ILE GLU SER SEQRES 45 B 601 LYS GLN LYS LYS ILE GLU ASN GLU ILE ALA ARG ILE LYS SEQRES 46 B 601 LYS LEU ILE GLY GLU ARG SER ALA TRP SER HIS PRO GLN SEQRES 47 B 601 PHE GLU LYS SEQRES 1 C 601 MET PRO MET GLY SER LEU GLN PRO LEU ALA THR LEU TYR SEQRES 2 C 601 LEU LEU GLY MET LEU VAL ALA SER VAL LEU GLY ASP ARG SEQRES 3 C 601 ILE CYS THR GLY ILE THR SER SER ASN SER PRO HIS VAL SEQRES 4 C 601 VAL LYS THR ALA THR GLN GLY GLU VAL ASN VAL THR GLY SEQRES 5 C 601 VAL ILE PRO LEU THR THR THR PRO THR LYS SER HIS PHE SEQRES 6 C 601 ALA ASN LEU LYS GLY THR GLU THR ARG GLY LYS LEU CYS SEQRES 7 C 601 PRO LYS CYS LEU ASN CYS THR ASP LEU ASP VAL ALA LEU SEQRES 8 C 601 GLY ARG PRO LYS CYS THR GLY LYS ILE PRO SER ALA ARG SEQRES 9 C 601 VAL SER ILE LEU HIS GLU VAL ARG PRO VAL THR SER GLY SEQRES 10 C 601 CYS PHE PRO ILE MET HIS ASP ARG THR LYS ILE ARG GLN SEQRES 11 C 601 LEU PRO ASN LEU LEU ARG GLY TYR GLU HIS VAL ARG LEU SEQRES 12 C 601 SER THR HIS ASN VAL ILE ASN ALA GLU ASP ALA PRO GLY SEQRES 13 C 601 ARG PRO TYR GLU ILE GLY THR SER GLY SER CYS PRO ASN SEQRES 14 C 601 ILE THR ASN GLY ASN GLY PHE PHE ALA THR MET ALA TRP SEQRES 15 C 601 ALA VAL PRO LYS ASN LYS THR ALA THR ASN PRO LEU THR SEQRES 16 C 601 ILE GLU VAL PRO TYR ILE CYS THR GLU GLY GLU ASP GLN SEQRES 17 C 601 ILE THR VAL TRP GLY PHE HIS SER ASP ASN GLU THR GLN SEQRES 18 C 601 MET ALA LYS LEU TYR GLY ASP SER LYS PRO GLN LYS PHE SEQRES 19 C 601 THR SER SER ALA ASN GLY VAL THR THR HIS TYR VAL SER SEQRES 20 C 601 GLN ILE GLY GLY PHE PRO ASN GLN THR GLU ASP GLY GLY SEQRES 21 C 601 LEU PRO GLN SER GLY ARG ILE VAL VAL ASP TYR MET VAL SEQRES 22 C 601 GLN LYS SER GLY LYS THR GLY THR ILE THR TYR GLN ARG SEQRES 23 C 601 GLY ILE LEU LEU PRO GLN LYS VAL TRP CYS ALA SER GLY SEQRES 24 C 601 ARG SER LYS VAL ILE LYS GLY SER LEU PRO LEU ILE GLY SEQRES 25 C 601 GLU ALA ASP CYS LEU HIS GLU LYS TYR GLY GLY LEU ASN SEQRES 26 C 601 LYS SER LYS PRO TYR TYR THR GLY GLU HIS ALA LYS ALA SEQRES 27 C 601 ILE GLY ASN CYS PRO ILE TRP VAL LYS THR PRO LEU LYS SEQRES 28 C 601 LEU ALA ASN GLY THR LYS TYR ARG PRO PRO ALA LYS LEU SEQRES 29 C 601 LEU LYS GLU ARG GLY PHE PHE GLY ALA ILE ALA GLY PHE SEQRES 30 C 601 LEU GLU GLY GLY TRP GLU GLY MET ILE ALA GLY TRP HIS SEQRES 31 C 601 GLY TYR THR SER HIS GLY ALA HIS GLY VAL ALA VAL ALA SEQRES 32 C 601 ALA ASP LEU LYS SER THR GLN GLU ALA ILE ASN LYS ILE SEQRES 33 C 601 THR LYS ASN LEU ASN SER LEU SER GLU LEU GLU VAL LYS SEQRES 34 C 601 ASN LEU GLN ARG LEU SER GLY ALA MET ASP GLU LEU HIS SEQRES 35 C 601 ASN GLU ILE LEU GLU LEU ASP GLU LYS VAL ASP ASP LEU SEQRES 36 C 601 ARG ALA ASP THR ILE SER SER GLN ILE GLU LEU ALA VAL SEQRES 37 C 601 LEU LEU SER ASN GLU GLY ILE ILE ASN SER GLU ASP GLU SEQRES 38 C 601 HIS LEU LEU ALA LEU GLU ARG LYS LEU LYS LYS MET LEU SEQRES 39 C 601 GLY PRO SER ALA VAL GLU ILE GLY ASN GLY CYS PHE GLU SEQRES 40 C 601 THR LYS HIS LYS CYS ASN GLN THR CYS LEU ASP ARG ILE SEQRES 41 C 601 ALA ALA GLY THR PHE ASP ALA GLY GLU PHE SER LEU PRO SEQRES 42 C 601 THR PHE ASP SER LEU ASN ILE THR ALA ALA SER LEU ASN SEQRES 43 C 601 ASP ASP GLY LEU ASP ASN HIS THR ILE SER GLY LYS ARG SEQRES 44 C 601 MET LYS GLN ILE GLU ASP LYS ILE GLU GLU ILE GLU SER SEQRES 45 C 601 LYS GLN LYS LYS ILE GLU ASN GLU ILE ALA ARG ILE LYS SEQRES 46 C 601 LYS LEU ILE GLY GLU ARG SER ALA TRP SER HIS PRO GLN SEQRES 47 C 601 PHE GLU LYS SEQRES 1 D 124 ASP VAL GLN LEU VAL GLU SER GLY GLY GLY LEU VAL GLN SEQRES 2 D 124 PRO GLY GLY SER LEU ARG LEU SER CYS ALA ALA SER GLY SEQRES 3 D 124 LEU VAL ASP SER ALA ASN VAL ALA TRP PHE ARG GLN ALA SEQRES 4 D 124 PRO GLY LYS GLU ARG GLU PHE VAL ALA ALA VAL LYS TRP SEQRES 5 D 124 ARG SER GLY SER THR ILE TYR ALA ASP SER VAL GLU GLY SEQRES 6 D 124 ARG PHE THR ILE SER ARG ASP ASN ALA LYS SER THR VAL SEQRES 7 D 124 TYR LEU GLN MET ASN SER LEU ARG PRO GLU ASP THR ALA SEQRES 8 D 124 VAL TYR TYR CYS ALA ALA ASN GLN TRP TYR SER GLY GLY SEQRES 9 D 124 TYR TYR GLY GLU LYS ASN TYR ASP TYR TRP GLY GLN GLY SEQRES 10 D 124 THR LEU VAL THR VAL SER SER SEQRES 1 E 124 ASP VAL GLN LEU VAL GLU SER GLY GLY GLY LEU VAL GLN SEQRES 2 E 124 PRO GLY GLY SER LEU ARG LEU SER CYS ALA ALA SER GLY SEQRES 3 E 124 LEU VAL ASP SER ALA ASN VAL ALA TRP PHE ARG GLN ALA SEQRES 4 E 124 PRO GLY LYS GLU ARG GLU PHE VAL ALA ALA VAL LYS TRP SEQRES 5 E 124 ARG SER GLY SER THR ILE TYR ALA ASP SER VAL GLU GLY SEQRES 6 E 124 ARG PHE THR ILE SER ARG ASP ASN ALA LYS SER THR VAL SEQRES 7 E 124 TYR LEU GLN MET ASN SER LEU ARG PRO GLU ASP THR ALA SEQRES 8 E 124 VAL TYR TYR CYS ALA ALA ASN GLN TRP TYR SER GLY GLY SEQRES 9 E 124 TYR TYR GLY GLU LYS ASN TYR ASP TYR TRP GLY GLN GLY SEQRES 10 E 124 THR LEU VAL THR VAL SER SER SEQRES 1 F 124 ASP VAL GLN LEU VAL GLU SER GLY GLY GLY LEU VAL GLN SEQRES 2 F 124 PRO GLY GLY SER LEU ARG LEU SER CYS ALA ALA SER GLY SEQRES 3 F 124 LEU VAL ASP SER ALA ASN VAL ALA TRP PHE ARG GLN ALA SEQRES 4 F 124 PRO GLY LYS GLU ARG GLU PHE VAL ALA ALA VAL LYS TRP SEQRES 5 F 124 ARG SER GLY SER THR ILE TYR ALA ASP SER VAL GLU GLY SEQRES 6 F 124 ARG PHE THR ILE SER ARG ASP ASN ALA LYS SER THR VAL SEQRES 7 F 124 TYR LEU GLN MET ASN SER LEU ARG PRO GLU ASP THR ALA SEQRES 8 F 124 VAL TYR TYR CYS ALA ALA ASN GLN TRP TYR SER GLY GLY SEQRES 9 F 124 TYR TYR GLY GLU LYS ASN TYR ASP TYR TRP GLY GLN GLY SEQRES 10 F 124 THR LEU VAL THR VAL SER SER HET NAG A 601 14 HET NAG A 602 14 HET NAG B 601 14 HET NAG B 602 14 HET NAG C 601 14 HET NAG C 602 14 HETNAM NAG 2-ACETAMIDO-2-DEOXY-BETA-D-GLUCOPYRANOSE HETSYN NAG N-ACETYL-BETA-D-GLUCOSAMINE; 2-ACETAMIDO-2-DEOXY-BETA- HETSYN 2 NAG D-GLUCOSE; 2-ACETAMIDO-2-DEOXY-D-GLUCOSE; 2-ACETAMIDO- HETSYN 3 NAG 2-DEOXY-GLUCOSE; N-ACETYL-D-GLUCOSAMINE FORMUL 7 NAG 6(C8 H15 N O6) HELIX 1 AA1 THR A 61 LEU A 67 1 7 HELIX 2 AA2 MET A 98 THR A 102 5 5 HELIX 3 AA3 LYS A 103 ARG A 105 5 3 HELIX 4 AA4 GLN A 106 GLY A 113 1 8 HELIX 5 AA5 ASN A 126 ALA A 130 5 5 HELIX 6 AA6 ASN A 194 GLY A 203 1 10 HELIX 7 AA7 LYS A 391 GLU A 401 1 11 HELIX 8 AA8 ASN A 419 ASN A 453 1 35 HELIX 9 AA9 THR B 61 GLY B 68 1 8 HELIX 10 AB1 MET B 98 THR B 102 5 5 HELIX 11 AB2 LYS B 103 ARG B 105 5 3 HELIX 12 AB3 GLN B 106 ARG B 112 1 7 HELIX 13 AB4 ASN B 126 ALA B 130 5 5 HELIX 14 AB5 ASN B 194 TYR B 202 1 9 HELIX 15 AB6 LYS B 391 GLU B 401 1 11 HELIX 16 AB7 ASN B 419 ASN B 453 1 35 HELIX 17 AB8 THR C 61 LEU C 67 1 7 HELIX 18 AB9 MET C 98 THR C 102 5 5 HELIX 19 AC1 LYS C 103 ARG C 105 5 3 HELIX 20 AC2 GLN C 106 GLY C 113 1 8 HELIX 21 AC3 ASN C 126 ALA C 130 5 5 HELIX 22 AC4 ASN C 194 TYR C 202 1 9 HELIX 23 AC5 LYS C 391 GLU C 401 1 11 HELIX 24 AC6 HIS C 418 ASN C 453 1 36 HELIX 25 AC7 ASP D 61 GLU D 64 5 4 HELIX 26 AC8 ARG D 83 THR D 87 5 5 HELIX 27 AC9 ASP E 61 GLU E 64 5 4 HELIX 28 AD1 ARG E 83 THR E 87 5 5 HELIX 29 AD2 ASP F 61 GLU F 64 5 4 HELIX 30 AD3 ARG F 83 THR F 87 5 5 SHEET 1 AA1 2 HIS A 14 THR A 18 0 SHEET 2 AA1 2 GLY A 22 VAL A 26 -1 O VAL A 26 N HIS A 14 SHEET 1 AA2 2 VAL A 29 PRO A 31 0 SHEET 2 AA2 2 LYS A 327 ALA A 329 -1 O LEU A 328 N ILE A 30 SHEET 1 AA3 2 ASN A 43 LEU A 44 0 SHEET 2 AA3 2 GLU A 289 ALA A 290 1 O ALA A 290 N ASN A 43 SHEET 1 AA4 3 THR A 49 ARG A 50 0 SHEET 2 AA4 3 VAL A 81 LEU A 84 1 O VAL A 81 N ARG A 50 SHEET 3 AA4 3 LYS A 278 LYS A 281 1 O LYS A 278 N SER A 82 SHEET 1 AA5 6 HIS A 116 LEU A 119 0 SHEET 2 AA5 6 TRP A 271 SER A 274 -1 O SER A 274 N HIS A 116 SHEET 3 AA5 6 ASP A 183 SER A 192 -1 N ILE A 185 O TRP A 271 SHEET 4 AA5 6 ILE A 264 PRO A 267 -1 O LEU A 265 N GLY A 189 SHEET 5 AA5 6 MET A 156 PRO A 161 -1 N ALA A 159 O ILE A 264 SHEET 6 AA5 6 TYR A 135 ILE A 137 -1 N GLU A 136 O VAL A 160 SHEET 1 AA6 4 HIS A 116 LEU A 119 0 SHEET 2 AA6 4 TRP A 271 SER A 274 -1 O SER A 274 N HIS A 116 SHEET 3 AA6 4 ASP A 183 SER A 192 -1 N ILE A 185 O TRP A 271 SHEET 4 AA6 4 ARG A 242 GLN A 250 -1 O GLN A 250 N GLN A 184 SHEET 1 AA7 4 LEU A 170 VAL A 174 0 SHEET 2 AA7 4 GLY A 256 TYR A 260 -1 O GLY A 256 N VAL A 174 SHEET 3 AA7 4 LYS A 209 ALA A 214 -1 N THR A 211 O THR A 259 SHEET 4 AA7 4 VAL A 217 VAL A 222 -1 O TYR A 221 N PHE A 210 SHEET 1 AA8 3 GLY A 298 LEU A 300 0 SHEET 2 AA8 3 CYS A 292 HIS A 294 -1 N CYS A 292 O LEU A 300 SHEET 3 AA8 3 ALA A 314 GLY A 316 -1 O ILE A 315 N LEU A 293 SHEET 1 AA9 2 TYR A 306 THR A 308 0 SHEET 2 AA9 2 ILE A 320 VAL A 322 1 O VAL A 322 N TYR A 307 SHEET 1 AB1 2 HIS B 14 VAL B 16 0 SHEET 2 AB1 2 VAL B 24 VAL B 26 -1 O VAL B 24 N VAL B 16 SHEET 1 AB2 2 VAL B 29 PRO B 31 0 SHEET 2 AB2 2 LYS B 327 ALA B 329 -1 O LEU B 328 N ILE B 30 SHEET 1 AB3 2 ASN B 43 LEU B 44 0 SHEET 2 AB3 2 GLU B 289 ALA B 290 1 O ALA B 290 N ASN B 43 SHEET 1 AB4 3 THR B 49 ARG B 50 0 SHEET 2 AB4 3 VAL B 81 LEU B 84 1 O VAL B 81 N ARG B 50 SHEET 3 AB4 3 LYS B 278 LYS B 281 1 O LYS B 278 N SER B 82 SHEET 1 AB5 6 HIS B 116 LEU B 119 0 SHEET 2 AB5 6 TRP B 271 SER B 274 -1 O SER B 274 N HIS B 116 SHEET 3 AB5 6 ASP B 183 SER B 192 -1 N ILE B 185 O TRP B 271 SHEET 4 AB5 6 ILE B 264 PRO B 267 -1 O LEU B 265 N GLY B 189 SHEET 5 AB5 6 MET B 156 PRO B 161 -1 N ALA B 157 O LEU B 266 SHEET 6 AB5 6 TYR B 135 ILE B 137 -1 N GLU B 136 O VAL B 160 SHEET 1 AB6 4 HIS B 116 LEU B 119 0 SHEET 2 AB6 4 TRP B 271 SER B 274 -1 O SER B 274 N HIS B 116 SHEET 3 AB6 4 ASP B 183 SER B 192 -1 N ILE B 185 O TRP B 271 SHEET 4 AB6 4 ARG B 242 GLN B 250 -1 O GLN B 250 N GLN B 184 SHEET 1 AB7 4 LEU B 170 VAL B 174 0 SHEET 2 AB7 4 GLY B 256 TYR B 260 -1 O GLY B 256 N VAL B 174 SHEET 3 AB7 4 LYS B 209 ALA B 214 -1 N THR B 211 O THR B 259 SHEET 4 AB7 4 VAL B 217 VAL B 222 -1 O TYR B 221 N PHE B 210 SHEET 1 AB8 3 GLY B 298 LEU B 300 0 SHEET 2 AB8 3 CYS B 292 HIS B 294 -1 N CYS B 292 O LEU B 300 SHEET 3 AB8 3 ALA B 314 GLY B 316 -1 O ILE B 315 N LEU B 293 SHEET 1 AB9 2 TYR B 306 THR B 308 0 SHEET 2 AB9 2 ILE B 320 VAL B 322 1 O VAL B 322 N TYR B 307 SHEET 1 AC1 2 HIS C 14 VAL C 16 0 SHEET 2 AC1 2 VAL C 24 VAL C 26 -1 O VAL C 26 N HIS C 14 SHEET 1 AC2 2 VAL C 29 PRO C 31 0 SHEET 2 AC2 2 LYS C 327 ALA C 329 -1 O LEU C 328 N ILE C 30 SHEET 1 AC3 2 ASN C 43 LEU C 44 0 SHEET 2 AC3 2 GLU C 289 ALA C 290 1 O ALA C 290 N ASN C 43 SHEET 1 AC4 3 THR C 49 ARG C 50 0 SHEET 2 AC4 3 VAL C 81 LEU C 84 1 O VAL C 81 N ARG C 50 SHEET 3 AC4 3 LYS C 278 LYS C 281 1 O LYS C 278 N SER C 82 SHEET 1 AC5 6 HIS C 116 LEU C 119 0 SHEET 2 AC5 6 TRP C 271 SER C 274 -1 O SER C 274 N HIS C 116 SHEET 3 AC5 6 ASP C 183 SER C 192 -1 N ILE C 185 O TRP C 271 SHEET 4 AC5 6 ILE C 264 PRO C 267 -1 O LEU C 265 N GLY C 189 SHEET 5 AC5 6 MET C 156 PRO C 161 -1 N ALA C 159 O ILE C 264 SHEET 6 AC5 6 TYR C 135 ILE C 137 -1 N GLU C 136 O VAL C 160 SHEET 1 AC6 4 HIS C 116 LEU C 119 0 SHEET 2 AC6 4 TRP C 271 SER C 274 -1 O SER C 274 N HIS C 116 SHEET 3 AC6 4 ASP C 183 SER C 192 -1 N ILE C 185 O TRP C 271 SHEET 4 AC6 4 ARG C 242 GLN C 250 -1 O GLN C 250 N GLN C 184 SHEET 1 AC7 4 LEU C 170 VAL C 174 0 SHEET 2 AC7 4 GLY C 256 TYR C 260 -1 O GLY C 256 N VAL C 174 SHEET 3 AC7 4 LYS C 209 ALA C 214 -1 N THR C 211 O THR C 259 SHEET 4 AC7 4 VAL C 217 VAL C 222 -1 O TYR C 221 N PHE C 210 SHEET 1 AC8 3 GLY C 298 LEU C 300 0 SHEET 2 AC8 3 CYS C 292 HIS C 294 -1 N CYS C 292 O LEU C 300 SHEET 3 AC8 3 ALA C 314 GLY C 316 -1 O ILE C 315 N LEU C 293 SHEET 1 AC9 2 TYR C 306 THR C 308 0 SHEET 2 AC9 2 ILE C 320 VAL C 322 1 O VAL C 322 N TYR C 307 SHEET 1 AD1 4 GLN D 3 SER D 7 0 SHEET 2 AD1 4 SER D 17 SER D 25 -1 O SER D 21 N SER D 7 SHEET 3 AD1 4 THR D 77 ASN D 82A-1 O MET D 82 N LEU D 18 SHEET 4 AD1 4 THR D 68 ASP D 72 -1 N SER D 70 O TYR D 79 SHEET 1 AD2 6 GLY D 10 VAL D 12 0 SHEET 2 AD2 6 THR D 107 VAL D 111 1 O THR D 110 N GLY D 10 SHEET 3 AD2 6 ALA D 88 ASN D 95 -1 N ALA D 88 O VAL D 109 SHEET 4 AD2 6 ASN D 32 GLN D 39 -1 N ALA D 34 O ALA D 93 SHEET 5 AD2 6 GLU D 46 LYS D 52 -1 O VAL D 51 N VAL D 33 SHEET 6 AD2 6 THR D 57 TYR D 59 -1 O ILE D 58 N ALA D 50 SHEET 1 AD3 4 GLY D 10 VAL D 12 0 SHEET 2 AD3 4 THR D 107 VAL D 111 1 O THR D 110 N GLY D 10 SHEET 3 AD3 4 ALA D 88 ASN D 95 -1 N ALA D 88 O VAL D 109 SHEET 4 AD3 4 TYR D 102 TRP D 103 -1 O TYR D 102 N ALA D 94 SHEET 1 AD4 4 GLN E 3 SER E 7 0 SHEET 2 AD4 4 SER E 17 SER E 25 -1 O SER E 21 N SER E 7 SHEET 3 AD4 4 THR E 77 ASN E 82A-1 O MET E 82 N LEU E 18 SHEET 4 AD4 4 THR E 68 ASP E 72 -1 N SER E 70 O TYR E 79 SHEET 1 AD5 6 GLY E 10 VAL E 12 0 SHEET 2 AD5 6 THR E 107 VAL E 111 1 O THR E 110 N GLY E 10 SHEET 3 AD5 6 ALA E 88 ASN E 95 -1 N ALA E 88 O VAL E 109 SHEET 4 AD5 6 ASN E 32 GLN E 39 -1 N ALA E 34 O ALA E 93 SHEET 5 AD5 6 GLU E 46 LYS E 52 -1 O VAL E 51 N VAL E 33 SHEET 6 AD5 6 THR E 57 TYR E 59 -1 O ILE E 58 N ALA E 50 SHEET 1 AD6 4 GLY E 10 VAL E 12 0 SHEET 2 AD6 4 THR E 107 VAL E 111 1 O THR E 110 N GLY E 10 SHEET 3 AD6 4 ALA E 88 ASN E 95 -1 N ALA E 88 O VAL E 109 SHEET 4 AD6 4 TYR E 102 TRP E 103 -1 O TYR E 102 N ALA E 94 SHEET 1 AD7 4 GLN F 3 SER F 7 0 SHEET 2 AD7 4 SER F 17 SER F 25 -1 O SER F 21 N SER F 7 SHEET 3 AD7 4 THR F 77 ASN F 82A-1 O MET F 82 N LEU F 18 SHEET 4 AD7 4 PHE F 67 ASP F 72 -1 N SER F 70 O TYR F 79 SHEET 1 AD8 6 GLY F 10 VAL F 12 0 SHEET 2 AD8 6 THR F 107 VAL F 111 1 O THR F 110 N GLY F 10 SHEET 3 AD8 6 ALA F 88 ASN F 95 -1 N ALA F 88 O VAL F 109 SHEET 4 AD8 6 ASN F 32 GLN F 39 -1 N ALA F 34 O ALA F 93 SHEET 5 AD8 6 GLU F 46 LYS F 52 -1 O VAL F 51 N VAL F 33 SHEET 6 AD8 6 THR F 57 TYR F 59 -1 O ILE F 58 N ALA F 50 SHEET 1 AD9 4 GLY F 10 VAL F 12 0 SHEET 2 AD9 4 THR F 107 VAL F 111 1 O THR F 110 N GLY F 10 SHEET 3 AD9 4 ALA F 88 ASN F 95 -1 N ALA F 88 O VAL F 109 SHEET 4 AD9 4 TYR F 102 TRP F 103 -1 O TYR F 102 N ALA F 94 SSBOND 1 CYS A 54 CYS A 57 1555 1555 2.03 SSBOND 2 CYS A 60 CYS A 72 1555 1555 2.03 SSBOND 3 CYS A 94 CYS A 143 1555 1555 2.04 SSBOND 4 CYS A 178 CYS A 272 1555 1555 2.04 SSBOND 5 CYS A 292 CYS A 318 1555 1555 2.03 SSBOND 6 CYS B 54 CYS B 57 1555 1555 2.03 SSBOND 7 CYS B 60 CYS B 72 1555 1555 2.03 SSBOND 8 CYS B 94 CYS B 143 1555 1555 2.04 SSBOND 9 CYS B 178 CYS B 272 1555 1555 2.04 SSBOND 10 CYS B 292 CYS B 318 1555 1555 2.03 SSBOND 11 CYS C 54 CYS C 57 1555 1555 2.03 SSBOND 12 CYS C 60 CYS C 72 1555 1555 2.03 SSBOND 13 CYS C 94 CYS C 143 1555 1555 2.04 SSBOND 14 CYS C 178 CYS C 272 1555 1555 2.04 SSBOND 15 CYS C 292 CYS C 318 1555 1555 2.03 SSBOND 16 CYS D 22 CYS D 92 1555 1555 2.03 SSBOND 17 CYS E 22 CYS E 92 1555 1555 2.04 SSBOND 18 CYS F 22 CYS F 92 1555 1555 2.03 LINK ND2 ASN A 145 C1 NAG A 601 1555 1555 1.44 LINK ND2 ASN A 194 C1 NAG A 602 1555 1555 1.44 LINK ND2 ASN B 145 C1 NAG B 601 1555 1555 1.44 LINK ND2 ASN B 194 C1 NAG B 602 1555 1555 1.44 LINK ND2 ASN C 145 C1 NAG C 601 1555 1555 1.44 LINK ND2 ASN C 194 C1 NAG C 602 1555 1555 1.44 CISPEP 1 ARG A 133 PRO A 134 0 8.06 CISPEP 2 LEU A 284 PRO A 285 0 -2.81 CISPEP 3 ARG B 133 PRO B 134 0 8.00 CISPEP 4 LEU B 284 PRO B 285 0 -2.76 CISPEP 5 ARG C 133 PRO C 134 0 8.28 CISPEP 6 LEU C 284 PRO C 285 0 -2.42 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000