HEADER IMMUNE SYSTEM 12-FEB-25 9NAQ TITLE CRYO-EM STRUCTURE OF 110_C4 FAB IN COMPLEX WITH CIDRA1.7 PFEMP1 COMPND MOL_ID: 1; COMPND 2 MOLECULE: 110_C4 FAB HEAVY CHAIN; COMPND 3 CHAIN: H; COMPND 4 ENGINEERED: YES; COMPND 5 MOL_ID: 2; COMPND 6 MOLECULE: 110_C4 FAB LIGHT CHAIN; COMPND 7 CHAIN: L; COMPND 8 ENGINEERED: YES; COMPND 9 MOL_ID: 3; COMPND 10 MOLECULE: ERYTHROCYTE MEMBRANE PROTEIN 1; COMPND 11 CHAIN: A; COMPND 12 ENGINEERED: YES; COMPND 13 OTHER_DETAILS: CIDRA1.7 PFEMP1 SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_TAXID: 9606; SOURCE 4 EXPRESSION_SYSTEM: CRICETULUS GRISEUS; SOURCE 5 EXPRESSION_SYSTEM_TAXID: 10029; SOURCE 6 MOL_ID: 2; SOURCE 7 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 8 ORGANISM_TAXID: 9606; SOURCE 9 EXPRESSION_SYSTEM: CRICETULUS GRISEUS; SOURCE 10 EXPRESSION_SYSTEM_TAXID: 10029; SOURCE 11 MOL_ID: 3; SOURCE 12 ORGANISM_SCIENTIFIC: PLASMODIUM FALCIPARUM; SOURCE 13 ORGANISM_COMMON: MALARIA PARASITE P. FALCIPARUM; SOURCE 14 ORGANISM_TAXID: 5833; SOURCE 15 GENE: IT4_VAR22; SOURCE 16 EXPRESSION_SYSTEM: SPODOPTERA FRUGIPERDA; SOURCE 17 EXPRESSION_SYSTEM_TAXID: 7108 KEYWDS SERUM ANTIBODY, CRYO-EM, MONOCLONAL ANTIBODY, PFEMP1, MALARIA, IMMUNE KEYWDS 2 SYSTEM EXPDTA ELECTRON MICROSCOPY AUTHOR S.S.R.RAGHAVAN,A.B.WARD REVDAT 1 27-AUG-25 9NAQ 0 JRNL AUTH L.TURNER,T.NUNEZ DE VILLAVICENCIO DIAZ,S.S.R.RAGHAVAN, JRNL AUTH 2 I.H.KANA,E.LYIMO,C.REITZEL,C.W.WANG,E.BERUBE,R.W.JENSEN, JRNL AUTH 3 J.R.LOEFFLER,M.L.FERNANDEZ-QUINTERO,T.G.THEANDER, JRNL AUTH 4 J.P.A.LUSINGU,T.LE BIHAN,X.HAN,D.T.R.MINJA,A.B.WARD,B.MA, JRNL AUTH 5 T.LAVSTSEN JRNL TITL IDENTIFICATION OF BROADLY INHIBITORY ANTI-PFEMP1 ANTIBODIES JRNL TITL 2 BY MASS SPECTROMETRY SEQUENCING OF PLASMA IGG FROM A JRNL TITL 3 MALARIA-EXPOSED CHILD. JRNL REF PROC.NATL.ACAD.SCI.USA V. 122 44122 2025 JRNL REFN ESSN 1091-6490 JRNL PMID 40833410 JRNL DOI 10.1073/PNAS.2508744122 REMARK 2 REMARK 2 RESOLUTION. 3.40 ANGSTROMS. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 SOFTWARE PACKAGES : PHENIX REMARK 3 RECONSTRUCTION SCHEMA : NULL REMARK 3 REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT REMARK 3 PDB ENTRY : NULL REMARK 3 REFINEMENT SPACE : NULL REMARK 3 REFINEMENT PROTOCOL : NULL REMARK 3 REFINEMENT TARGET : NULL REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL REMARK 3 REMARK 3 FITTING PROCEDURE : NULL REMARK 3 REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 3.400 REMARK 3 NUMBER OF PARTICLES : 394495 REMARK 3 CTF CORRECTION METHOD : PHASE FLIPPING AND AMPLITUDE REMARK 3 CORRECTION REMARK 3 REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL REMARK 3 REMARK 3 OTHER DETAILS: NULL REMARK 4 REMARK 4 9NAQ COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 13-FEB-25. REMARK 100 THE DEPOSITION ID IS D_1000292920. REMARK 245 REMARK 245 EXPERIMENTAL DETAILS REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE REMARK 245 SPECIMEN TYPE : NULL REMARK 245 REMARK 245 ELECTRON MICROSCOPE SAMPLE REMARK 245 SAMPLE TYPE : PARTICLE REMARK 245 PARTICLE TYPE : POINT REMARK 245 NAME OF SAMPLE : COMPLEX OF HUMAN SERUM ANTIBODY REMARK 245 110_C4 WITH CIDRA1.7 PFEMP1 REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : NULL REMARK 245 SAMPLE SUPPORT DETAILS : NULL REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL REMARK 245 SAMPLE BUFFER : NULL REMARK 245 PH : 7.50 REMARK 245 SAMPLE DETAILS : NULL REMARK 245 REMARK 245 DATA ACQUISITION REMARK 245 DATE OF EXPERIMENT : NULL REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : NULL REMARK 245 TEMPERATURE (KELVIN) : NULL REMARK 245 MICROSCOPE MODEL : TFS GLACIOS REMARK 245 DETECTOR TYPE : FEI FALCON IV (4K X 4K) REMARK 245 MINIMUM DEFOCUS (NM) : 1000.00 REMARK 245 MAXIMUM DEFOCUS (NM) : 2200.00 REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 NOMINAL CS : NULL REMARK 245 IMAGING MODE : BRIGHT FIELD REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 4900.00 REMARK 245 ILLUMINATION MODE : FLOOD BEAM REMARK 245 NOMINAL MAGNIFICATION : NULL REMARK 245 CALIBRATED MAGNIFICATION : NULL REMARK 245 SOURCE : FIELD EMISSION GUN REMARK 245 ACCELERATION VOLTAGE (KV) : 200 REMARK 245 IMAGING DETAILS : NULL REMARK 247 REMARK 247 ELECTRON MICROSCOPY REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION REMARK 247 OF THE STRUCTURE FACTORS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: TRIMERIC REMARK 350 APPLY THE FOLLOWING TO CHAINS: H, L, A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 REMARK 465 M RES C SSSEQI REMARK 465 MET A 1 REMARK 465 GLY A 2 REMARK 465 SER A 3 REMARK 465 GLN A 4 REMARK 465 SER A 5 REMARK 465 SER A 6 REMARK 465 LYS A 7 REMARK 465 PRO A 8 REMARK 465 SER A 9 REMARK 465 LYS A 10 REMARK 465 PRO A 11 REMARK 465 SER A 12 REMARK 465 VAL A 13 REMARK 465 ASP A 14 REMARK 465 THR A 15 REMARK 465 ASN A 16 REMARK 465 GLU A 17 REMARK 465 SER A 18 REMARK 465 TYR A 19 REMARK 465 LYS A 20 REMARK 465 SER A 21 REMARK 465 ALA A 22 REMARK 465 ARG A 23 REMARK 465 ASN A 24 REMARK 465 VAL A 25 REMARK 465 LEU A 26 REMARK 465 GLU A 27 REMARK 465 ARG A 28 REMARK 465 TYR A 29 REMARK 465 ALA A 30 REMARK 465 GLU A 31 REMARK 465 SER A 32 REMARK 465 ILE A 33 REMARK 465 LYS A 34 REMARK 465 GLN A 35 REMARK 465 GLN A 36 REMARK 465 ALA A 37 REMARK 465 GLU A 38 REMARK 465 ASN A 39 REMARK 465 ASP A 40 REMARK 465 ALA A 41 REMARK 465 SER A 42 REMARK 465 GLY A 43 REMARK 465 TYR A 44 REMARK 465 GLU A 45 REMARK 465 LYS A 46 REMARK 465 GLU A 47 REMARK 465 LEU A 48 REMARK 465 LYS A 49 REMARK 465 GLY A 50 REMARK 465 LYS A 51 REMARK 465 LEU A 52 REMARK 465 GLU A 53 REMARK 465 GLU A 54 REMARK 465 ALA A 55 REMARK 465 SER A 56 REMARK 465 PHE A 57 REMARK 465 CYS A 58 REMARK 465 GLY A 59 REMARK 465 ALA A 60 REMARK 465 TYR A 61 REMARK 465 CYS A 62 REMARK 465 GLU A 63 REMARK 465 LEU A 64 REMARK 465 ILE A 65 REMARK 465 GLY A 66 REMARK 465 VAL A 67 REMARK 465 PRO A 68 REMARK 465 LYS A 69 REMARK 465 TYR A 70 REMARK 465 GLY A 71 REMARK 465 SER A 72 REMARK 465 THR A 73 REMARK 465 ASP A 74 REMARK 465 PRO A 75 REMARK 465 CYS A 76 REMARK 465 TYR A 77 REMARK 465 LEU A 78 REMARK 465 ASP A 79 REMARK 465 HIS A 80 REMARK 465 ARG A 81 REMARK 465 TRP A 82 REMARK 465 HIS A 83 REMARK 465 THR A 84 REMARK 465 ASN A 85 REMARK 465 LEU A 86 REMARK 465 LEU A 87 REMARK 465 HIS A 88 REMARK 465 GLU A 89 REMARK 465 LYS A 90 REMARK 465 VAL A 91 REMARK 465 LYS A 92 REMARK 465 ASP A 93 REMARK 465 ARG A 94 REMARK 465 ASP A 95 REMARK 465 PRO A 96 REMARK 465 CYS A 97 REMARK 465 HIS A 98 REMARK 465 ASN A 99 REMARK 465 ARG A 100 REMARK 465 ASN A 101 REMARK 465 GLN A 102 REMARK 465 LYS A 103 REMARK 465 ARG A 104 REMARK 465 PHE A 105 REMARK 465 ASP A 106 REMARK 465 GLU A 107 REMARK 465 GLY A 108 REMARK 465 GLN A 109 REMARK 465 VAL A 110 REMARK 465 TYR A 111 REMARK 465 GLU A 112 REMARK 465 CYS A 113 REMARK 465 GLY A 114 REMARK 465 SER A 115 REMARK 465 GLY A 116 REMARK 465 ILE A 117 REMARK 465 ILE A 118 REMARK 465 LYS A 119 REMARK 465 GLY A 120 REMARK 465 ASN A 121 REMARK 465 GLY A 122 REMARK 465 ASN A 123 REMARK 465 ASN A 124 REMARK 465 ARG A 125 REMARK 465 ASN A 126 REMARK 465 GLY A 127 REMARK 465 GLY A 128 REMARK 465 SER A 129 REMARK 465 CYS A 130 REMARK 465 ALA A 131 REMARK 465 PRO A 132 REMARK 465 PRO A 133 REMARK 465 ARG A 134 REMARK 465 ARG A 135 REMARK 465 ARG A 136 REMARK 465 HIS A 137 REMARK 465 MET A 138 REMARK 465 CYS A 139 REMARK 465 ASP A 140 REMARK 465 LYS A 141 REMARK 465 ASN A 142 REMARK 465 LEU A 143 REMARK 465 GLU A 144 REMARK 465 ALA A 145 REMARK 465 LEU A 146 REMARK 465 THR A 147 REMARK 465 VAL A 148 REMARK 465 ALA A 149 REMARK 465 ASN A 150 REMARK 465 THR A 151 REMARK 465 LYS A 152 REMARK 465 ASN A 153 REMARK 465 SER A 154 REMARK 465 ASN A 155 REMARK 465 ASP A 156 REMARK 465 LEU A 157 REMARK 465 LEU A 158 REMARK 465 GLY A 159 REMARK 465 ASN A 160 REMARK 465 ILE A 161 REMARK 465 LEU A 162 REMARK 465 VAL A 163 REMARK 465 THR A 164 REMARK 465 ALA A 165 REMARK 465 LYS A 166 REMARK 465 TYR A 167 REMARK 465 GLU A 168 REMARK 465 GLY A 169 REMARK 465 ASP A 170 REMARK 465 SER A 171 REMARK 465 ILE A 172 REMARK 465 VAL A 173 REMARK 465 ASN A 174 REMARK 465 SER A 175 REMARK 465 TYR A 176 REMARK 465 ALA A 177 REMARK 465 ASN A 178 REMARK 465 SER A 179 REMARK 465 GLY A 180 REMARK 465 MET A 181 REMARK 465 PHE A 182 REMARK 465 ASN A 183 REMARK 465 VAL A 184 REMARK 465 CYS A 185 REMARK 465 THR A 186 REMARK 465 ALA A 187 REMARK 465 LEU A 188 REMARK 465 ALA A 189 REMARK 465 ARG A 190 REMARK 465 SER A 191 REMARK 465 PHE A 192 REMARK 465 ALA A 193 REMARK 465 ASP A 194 REMARK 465 ILE A 195 REMARK 465 GLY A 196 REMARK 465 ASP A 197 REMARK 465 ILE A 198 REMARK 465 ILE A 199 REMARK 465 ARG A 200 REMARK 465 GLY A 201 REMARK 465 LYS A 202 REMARK 465 ASP A 203 REMARK 465 LEU A 204 REMARK 465 TYR A 205 REMARK 465 LEU A 206 REMARK 465 GLY A 207 REMARK 465 ASN A 208 REMARK 465 GLY A 209 REMARK 465 ASP A 210 REMARK 465 TYR A 211 REMARK 465 LYS A 212 REMARK 465 GLU A 213 REMARK 465 LYS A 214 REMARK 465 VAL A 215 REMARK 465 SER A 216 REMARK 465 ASN A 217 REMARK 465 ASN A 218 REMARK 465 LEU A 219 REMARK 465 ARG A 220 REMARK 465 ALA A 221 REMARK 465 ILE A 222 REMARK 465 PHE A 223 REMARK 465 ASN A 224 REMARK 465 LYS A 225 REMARK 465 ILE A 226 REMARK 465 TYR A 227 REMARK 465 GLU A 228 REMARK 465 ASN A 229 REMARK 465 LEU A 230 REMARK 465 ASN A 231 REMARK 465 ASP A 232 REMARK 465 PRO A 233 REMARK 465 ASN A 234 REMARK 465 VAL A 235 REMARK 465 LYS A 236 REMARK 465 ALA A 237 REMARK 465 HIS A 238 REMARK 465 TYR A 239 REMARK 465 GLN A 240 REMARK 465 LYS A 241 REMARK 465 ASP A 242 REMARK 465 ALA A 243 REMARK 465 PRO A 244 REMARK 465 ASN A 245 REMARK 465 TYR A 246 REMARK 465 TYR A 247 REMARK 465 LYS A 248 REMARK 465 LEU A 249 REMARK 465 ARG A 250 REMARK 465 GLU A 251 REMARK 465 HIS A 252 REMARK 465 TRP A 253 REMARK 465 TRP A 254 REMARK 465 THR A 255 REMARK 465 VAL A 256 REMARK 465 ASN A 257 REMARK 465 ARG A 258 REMARK 465 ASP A 259 REMARK 465 GLN A 260 REMARK 465 VAL A 261 REMARK 465 TRP A 262 REMARK 465 LYS A 263 REMARK 465 ALA A 264 REMARK 465 ILE A 265 REMARK 465 THR A 266 REMARK 465 CYS A 267 REMARK 465 ASN A 268 REMARK 465 ALA A 269 REMARK 465 PRO A 270 REMARK 465 THR A 271 REMARK 465 GLY A 272 REMARK 465 ALA A 273 REMARK 465 ASP A 274 REMARK 465 TYR A 275 REMARK 465 PHE A 276 REMARK 465 ARG A 277 REMARK 465 LYS A 278 REMARK 465 GLY A 279 REMARK 465 SER A 280 REMARK 465 ASP A 281 REMARK 465 GLY A 282 REMARK 465 THR A 283 REMARK 465 ASN A 284 REMARK 465 VAL A 285 REMARK 465 PHE A 286 REMARK 465 THR A 287 REMARK 465 SER A 288 REMARK 465 GLN A 289 REMARK 465 GLY A 290 REMARK 465 GLN A 291 REMARK 465 CYS A 292 REMARK 465 GLY A 293 REMARK 465 HIS A 294 REMARK 465 TYR A 295 REMARK 465 GLU A 296 REMARK 465 GLY A 297 REMARK 465 ALA A 298 REMARK 465 PRO A 299 REMARK 465 PRO A 300 REMARK 465 THR A 301 REMARK 465 ASN A 302 REMARK 465 LEU A 303 REMARK 465 ASP A 304 REMARK 465 TYR A 305 REMARK 465 VAL A 306 REMARK 465 PRO A 307 REMARK 465 GLN A 308 REMARK 465 PHE A 309 REMARK 465 LEU A 310 REMARK 465 ARG A 311 REMARK 465 TRP A 312 REMARK 465 PHE A 313 REMARK 465 GLU A 314 REMARK 465 GLU A 315 REMARK 465 TRP A 316 REMARK 465 ALA A 317 REMARK 465 GLU A 318 REMARK 465 GLU A 319 REMARK 465 PHE A 320 REMARK 465 CYS A 321 REMARK 465 ARG A 322 REMARK 465 LYS A 323 REMARK 465 LYS A 324 REMARK 465 LYS A 325 REMARK 465 ILE A 326 REMARK 465 LYS A 327 REMARK 465 LEU A 328 REMARK 465 GLU A 329 REMARK 465 ASN A 330 REMARK 465 VAL A 331 REMARK 465 LYS A 332 REMARK 465 LYS A 333 REMARK 465 ALA A 334 REMARK 465 CYS A 335 REMARK 465 ARG A 336 REMARK 465 ASP A 337 REMARK 465 GLU A 338 REMARK 465 SER A 339 REMARK 465 SER A 340 REMARK 465 LYS A 341 REMARK 465 LEU A 342 REMARK 465 TYR A 343 REMARK 465 CYS A 344 REMARK 465 SER A 345 REMARK 465 HIS A 346 REMARK 465 ASN A 347 REMARK 465 GLY A 348 REMARK 465 TYR A 349 REMARK 465 ASP A 350 REMARK 465 CYS A 351 REMARK 465 THR A 352 REMARK 465 GLN A 353 REMARK 465 THR A 354 REMARK 465 ILE A 355 REMARK 465 ARG A 356 REMARK 465 ASN A 357 REMARK 465 LYS A 358 REMARK 465 ASP A 359 REMARK 465 ILE A 360 REMARK 465 CYS A 361 REMARK 465 ILE A 362 REMARK 465 ARG A 363 REMARK 465 GLU A 364 REMARK 465 SER A 365 REMARK 465 LYS A 366 REMARK 465 CYS A 367 REMARK 465 THR A 368 REMARK 465 ASP A 369 REMARK 465 CYS A 370 REMARK 465 SER A 371 REMARK 465 THR A 372 REMARK 465 LYS A 373 REMARK 465 CYS A 374 REMARK 465 LYS A 375 REMARK 465 LEU A 376 REMARK 465 TYR A 377 REMARK 465 GLU A 378 REMARK 465 LEU A 379 REMARK 465 TRP A 380 REMARK 465 LEU A 381 REMARK 465 GLU A 382 REMARK 465 LYS A 383 REMARK 465 GLN A 384 REMARK 465 GLU A 385 REMARK 465 ASN A 386 REMARK 465 GLU A 387 REMARK 465 PHE A 388 REMARK 465 LYS A 389 REMARK 465 LYS A 390 REMARK 465 GLN A 391 REMARK 465 THR A 392 REMARK 465 LYS A 393 REMARK 465 LYS A 394 REMARK 465 TYR A 395 REMARK 465 ASP A 396 REMARK 465 LYS A 397 REMARK 465 GLU A 398 REMARK 465 ILE A 399 REMARK 465 ASN A 400 REMARK 465 GLY A 401 REMARK 465 ASN A 402 REMARK 465 ASN A 403 REMARK 465 SER A 404 REMARK 465 LEU A 405 REMARK 465 GLN A 406 REMARK 465 ASN A 407 REMARK 465 ASN A 408 REMARK 465 LYS A 409 REMARK 465 ASN A 410 REMARK 465 ASN A 411 REMARK 465 GLY A 412 REMARK 465 ILE A 413 REMARK 465 ASP A 414 REMARK 465 LYS A 415 REMARK 465 LYS A 416 REMARK 465 TYR A 417 REMARK 465 HIS A 418 REMARK 465 ASN A 419 REMARK 465 GLU A 420 REMARK 465 PHE A 421 REMARK 465 TYR A 422 REMARK 465 LYS A 423 REMARK 465 ASN A 424 REMARK 465 PHE A 425 REMARK 465 ARG A 426 REMARK 465 GLU A 427 REMARK 465 LYS A 428 REMARK 465 GLY A 429 REMARK 465 TYR A 430 REMARK 465 THR A 431 REMARK 465 SER A 432 REMARK 465 LEU A 433 REMARK 465 ASP A 434 REMARK 465 LYS A 435 REMARK 465 PHE A 436 REMARK 465 LEU A 437 REMARK 465 LYS A 438 REMARK 465 LEU A 439 REMARK 465 LEU A 440 REMARK 465 ASN A 441 REMARK 465 GLU A 442 REMARK 465 GLY A 443 REMARK 465 MET A 444 REMARK 465 TYR A 445 REMARK 465 CYS A 446 REMARK 465 LYS A 447 REMARK 465 ASN A 448 REMARK 465 GLN A 449 REMARK 465 LYS A 450 REMARK 465 PRO A 451 REMARK 465 GLU A 452 REMARK 465 GLU A 453 REMARK 465 GLU A 454 REMARK 465 ASP A 455 REMARK 465 ILE A 456 REMARK 465 ASP A 457 REMARK 465 PHE A 458 REMARK 465 THR A 459 REMARK 465 LYS A 460 REMARK 465 ASN A 461 REMARK 465 GLY A 462 REMARK 465 ASP A 463 REMARK 465 LYS A 464 REMARK 465 GLY A 465 REMARK 465 ILE A 466 REMARK 465 PHE A 467 REMARK 465 TYR A 468 REMARK 465 ARG A 469 REMARK 465 SER A 470 REMARK 465 GLU A 471 REMARK 465 TYR A 472 REMARK 465 CYS A 473 REMARK 465 GLN A 474 REMARK 465 VAL A 475 REMARK 465 CYS A 476 REMARK 465 PRO A 477 REMARK 465 TYR A 478 REMARK 465 CYS A 479 REMARK 465 GLY A 480 REMARK 465 LEU A 481 REMARK 465 ASP A 482 REMARK 465 CYS A 483 REMARK 465 GLY A 484 REMARK 465 GLY A 485 REMARK 465 LYS A 486 REMARK 465 THR A 487 REMARK 465 CYS A 488 REMARK 465 THR A 489 REMARK 465 ALA A 490 REMARK 465 LYS A 491 REMARK 465 GLN A 492 REMARK 465 GLU A 493 REMARK 465 ILE A 494 REMARK 465 TYR A 495 REMARK 465 PRO A 496 REMARK 465 ASP A 497 REMARK 465 CYS A 498 REMARK 465 VAL A 499 REMARK 465 TYR A 500 REMARK 465 ASN A 501 REMARK 465 GLY A 502 REMARK 465 ALA A 503 REMARK 465 TYR A 504 REMARK 465 GLU A 505 REMARK 465 PRO A 506 REMARK 465 PRO A 507 REMARK 465 ASN A 508 REMARK 465 GLY A 509 REMARK 465 ALA A 510 REMARK 465 GLU A 511 REMARK 465 THR A 512 REMARK 465 THR A 513 REMARK 465 GLU A 514 REMARK 465 ILE A 515 REMARK 465 THR A 516 REMARK 465 VAL A 517 REMARK 465 LEU A 518 REMARK 465 TYR A 519 REMARK 465 SER A 520 REMARK 465 ALA A 521 REMARK 465 ASP A 522 REMARK 465 GLN A 523 REMARK 465 GLU A 524 REMARK 465 GLY A 525 REMARK 465 ASP A 526 REMARK 465 ILE A 527 REMARK 465 SER A 528 REMARK 465 ASN A 529 REMARK 465 LYS A 530 REMARK 465 LEU A 531 REMARK 465 SER A 532 REMARK 465 GLU A 533 REMARK 465 PHE A 534 REMARK 465 CYS A 535 REMARK 465 ASN A 536 REMARK 465 ASP A 537 REMARK 465 GLU A 538 REMARK 465 ASN A 539 REMARK 465 ASN A 540 REMARK 465 LYS A 541 REMARK 465 ASN A 542 REMARK 465 SER A 543 REMARK 465 GLN A 544 REMARK 465 LYS A 545 REMARK 465 TRP A 546 REMARK 465 GLN A 547 REMARK 465 CYS A 548 REMARK 465 TYR A 549 REMARK 465 TYR A 550 REMARK 465 VAL A 551 REMARK 465 SER A 552 REMARK 465 SER A 553 REMARK 465 GLU A 554 REMARK 465 ASN A 555 REMARK 465 ASN A 556 REMARK 465 GLY A 557 REMARK 465 CYS A 558 REMARK 465 LYS A 559 REMARK 465 MET A 560 REMARK 465 GLU A 561 REMARK 465 LYS A 562 REMARK 465 LYS A 563 REMARK 465 ASN A 564 REMARK 465 ALA A 565 REMARK 465 ASN A 566 REMARK 465 HIS A 567 REMARK 465 THR A 568 REMARK 465 PRO A 569 REMARK 465 GLU A 570 REMARK 465 VAL A 571 REMARK 465 LYS A 572 REMARK 465 ILE A 573 REMARK 465 THR A 574 REMARK 465 CYS A 601 REMARK 465 MET A 602 REMARK 465 ASN A 603 REMARK 465 ASN A 604 REMARK 465 THR A 605 REMARK 465 LYS A 606 REMARK 465 THR A 607 REMARK 465 ALA A 608 REMARK 465 ASP A 609 REMARK 465 CYS A 610 REMARK 465 ILE A 611 REMARK 465 HIS A 612 REMARK 465 GLU A 613 REMARK 465 CYS A 614 REMARK 465 ASN A 615 REMARK 465 LYS A 616 REMARK 465 ASN A 617 REMARK 465 CYS A 618 REMARK 465 LYS A 637 REMARK 465 LEU A 638 REMARK 465 PHE A 639 REMARK 465 THR A 640 REMARK 465 LYS A 641 REMARK 465 GLU A 642 REMARK 465 LYS A 643 REMARK 465 LYS A 644 REMARK 465 MET A 645 REMARK 465 PRO A 646 REMARK 465 LYS A 647 REMARK 465 GLN A 648 REMARK 465 TYR A 649 REMARK 465 TYR A 650 REMARK 465 GLY A 651 REMARK 465 ASN A 652 REMARK 465 ASP A 679 REMARK 465 GLU A 680 REMARK 465 LEU A 681 REMARK 465 ARG A 682 REMARK 465 ASN A 683 REMARK 465 LYS A 684 REMARK 465 ILE A 685 REMARK 465 GLU A 686 REMARK 465 LEU A 687 REMARK 465 SER A 688 REMARK 465 LYS A 689 REMARK 465 GLY A 690 REMARK 465 ASN A 691 REMARK 465 GLU A 692 REMARK 465 GLY A 693 REMARK 465 THR A 694 REMARK 465 LYS A 695 REMARK 465 ASP A 696 REMARK 465 LEU A 697 REMARK 465 GLN A 698 REMARK 465 CYS A 715 REMARK 465 LYS A 716 REMARK 465 ASP A 717 REMARK 465 ASN A 718 REMARK 465 ASN A 719 REMARK 465 THR A 720 REMARK 465 ASN A 721 REMARK 465 GLU A 722 REMARK 465 ALA A 723 REMARK 465 CYS A 724 REMARK 465 ASP A 725 REMARK 465 PRO A 726 REMARK 465 THR A 727 REMARK 465 VAL A 728 REMARK 465 ASP A 729 REMARK 465 PRO A 730 REMARK 465 THR A 731 REMARK 465 LYS A 732 REMARK 465 ASN A 733 REMARK 465 PRO A 734 REMARK 465 CYS A 735 REMARK 465 GLY A 736 REMARK 465 LYS A 737 REMARK 465 ASN A 738 REMARK 465 THR A 739 REMARK 465 LYS A 740 REMARK 465 ALA A 741 REMARK 465 GLY A 742 REMARK 465 SER A 743 REMARK 465 ASP A 744 REMARK 465 LYS A 745 REMARK 465 VAL A 746 REMARK 465 ILE A 747 REMARK 465 SER A 748 REMARK 465 VAL A 749 REMARK 465 LYS A 750 REMARK 465 GLN A 751 REMARK 465 ILE A 752 REMARK 465 ALA A 753 REMARK 465 GLN A 754 REMARK 465 TYR A 755 REMARK 465 TYR A 756 REMARK 465 LYS A 757 REMARK 465 ARG A 758 REMARK 465 LEU A 759 REMARK 465 ALA A 760 REMARK 465 HIS A 761 REMARK 465 GLU A 762 REMARK 465 GLN A 763 REMARK 465 LEU A 764 REMARK 465 GLU A 765 REMARK 465 GLU A 766 REMARK 465 ARG A 767 REMARK 465 GLY A 768 REMARK 465 SER A 769 REMARK 465 ARG A 770 REMARK 465 SER A 771 REMARK 465 ALA A 772 REMARK 465 LEU A 773 REMARK 465 LYS A 774 REMARK 465 GLY A 775 REMARK 465 ASP A 776 REMARK 465 ALA A 777 REMARK 465 SER A 778 REMARK 465 LYS A 779 REMARK 465 GLY A 780 REMARK 465 THR A 781 REMARK 465 TYR A 782 REMARK 465 ARG A 783 REMARK 465 ARG A 784 REMARK 465 GLN A 785 REMARK 465 GLY A 786 REMARK 465 ASN A 787 REMARK 465 PRO A 788 REMARK 465 ARG A 789 REMARK 465 LYS A 790 REMARK 465 LEU A 791 REMARK 465 LYS A 792 REMARK 465 LYS A 793 REMARK 465 VAL A 794 REMARK 465 CYS A 795 REMARK 465 ARG A 796 REMARK 465 ILE A 797 REMARK 465 ALA A 798 REMARK 465 LYS A 799 REMARK 465 ASP A 800 REMARK 465 HIS A 801 REMARK 465 SER A 802 REMARK 465 ASN A 803 REMARK 465 ARG A 804 REMARK 465 ASN A 805 REMARK 465 HIS A 806 REMARK 465 LYS A 807 REMARK 465 ASP A 808 REMARK 465 SER A 809 REMARK 465 ARG A 810 REMARK 465 GLY A 811 REMARK 465 ARG A 812 REMARK 465 HIS A 813 REMARK 465 LEU A 814 REMARK 465 CYS A 815 REMARK 465 THR A 816 REMARK 465 SER A 817 REMARK 465 TYR A 818 REMARK 465 LEU A 819 REMARK 465 GLU A 820 REMARK 465 PHE A 821 REMARK 465 LEU A 822 REMARK 465 GLN A 823 REMARK 465 THR A 824 REMARK 465 ILE A 825 REMARK 465 ASP A 826 REMARK 465 ASP A 827 REMARK 465 SER A 828 REMARK 465 HIS A 829 REMARK 465 ASN A 830 REMARK 465 SER A 831 REMARK 465 SER A 832 REMARK 465 ASN A 833 REMARK 465 ALA A 834 REMARK 465 LYS A 835 REMARK 465 ARG A 836 REMARK 465 VAL A 837 REMARK 465 ASN A 838 REMARK 465 ASN A 839 REMARK 465 SER A 840 REMARK 465 PHE A 841 REMARK 465 LEU A 842 REMARK 465 GLY A 843 REMARK 465 ASP A 844 REMARK 465 VAL A 845 REMARK 465 LEU A 846 REMARK 465 LEU A 847 REMARK 465 SER A 848 REMARK 465 ALA A 849 REMARK 465 LYS A 850 REMARK 465 LEU A 851 REMARK 465 ASP A 852 REMARK 465 ALA A 853 REMARK 465 ALA A 854 REMARK 465 GLU A 855 REMARK 465 ILE A 856 REMARK 465 ILE A 857 REMARK 465 LYS A 858 REMARK 465 ARG A 859 REMARK 465 TYR A 860 REMARK 465 LYS A 861 REMARK 465 ASP A 862 REMARK 465 GLN A 863 REMARK 465 ASN A 864 REMARK 465 ASN A 865 REMARK 465 ILE A 866 REMARK 465 ARG A 867 REMARK 465 GLU A 868 REMARK 465 ASN A 869 REMARK 465 ILE A 870 REMARK 465 GLU A 871 REMARK 465 GLN A 872 REMARK 465 LYS A 873 REMARK 465 ASP A 874 REMARK 465 GLU A 875 REMARK 465 GLU A 876 REMARK 465 ALA A 877 REMARK 465 MET A 878 REMARK 465 CYS A 879 REMARK 465 ARG A 880 REMARK 465 ALA A 881 REMARK 465 VAL A 882 REMARK 465 ARG A 883 REMARK 465 TYR A 884 REMARK 465 SER A 885 REMARK 465 PHE A 886 REMARK 465 ALA A 887 REMARK 465 ASP A 888 REMARK 465 LEU A 889 REMARK 465 GLY A 890 REMARK 465 ASP A 891 REMARK 465 ILE A 892 REMARK 465 ILE A 893 REMARK 465 ARG A 894 REMARK 465 GLY A 895 REMARK 465 LYS A 896 REMARK 465 ASP A 897 REMARK 465 LEU A 898 REMARK 465 TRP A 899 REMARK 465 ASP A 900 REMARK 465 HIS A 901 REMARK 465 LYS A 902 REMARK 465 ASP A 903 REMARK 465 PHE A 904 REMARK 465 LYS A 905 REMARK 465 LYS A 906 REMARK 465 LEU A 907 REMARK 465 GLU A 908 REMARK 465 ARG A 909 REMARK 465 ASP A 910 REMARK 465 LEU A 911 REMARK 465 VAL A 912 REMARK 465 LYS A 913 REMARK 465 ILE A 914 REMARK 465 PHE A 915 REMARK 465 GLY A 916 REMARK 465 LYS A 917 REMARK 465 ILE A 918 REMARK 465 LYS A 919 REMARK 465 ASP A 920 REMARK 465 GLU A 921 REMARK 465 LEU A 922 REMARK 465 LYS A 923 REMARK 465 SER A 924 REMARK 465 LYS A 925 REMARK 465 LEU A 926 REMARK 465 GLY A 927 REMARK 465 ASP A 928 REMARK 465 LYS A 929 REMARK 465 TYR A 930 REMARK 465 ILE A 931 REMARK 465 GLY A 932 REMARK 465 ASP A 933 REMARK 465 GLU A 934 REMARK 465 ALA A 935 REMARK 465 LYS A 936 REMARK 465 SER A 937 REMARK 465 PRO A 938 REMARK 465 TYR A 939 REMARK 465 LYS A 940 REMARK 465 GLN A 941 REMARK 465 LEU A 942 REMARK 465 ARG A 943 REMARK 465 SER A 944 REMARK 465 ASP A 945 REMARK 465 TRP A 946 REMARK 465 TRP A 947 REMARK 465 GLU A 948 REMARK 465 ALA A 949 REMARK 465 ASN A 950 REMARK 465 ARG A 951 REMARK 465 HIS A 952 REMARK 465 GLN A 953 REMARK 465 VAL A 954 REMARK 465 TRP A 955 REMARK 465 LYS A 956 REMARK 465 ALA A 957 REMARK 465 MET A 958 REMARK 465 GLN A 959 REMARK 465 CYS A 960 REMARK 465 LYS A 961 REMARK 465 THR A 962 REMARK 465 THR A 963 REMARK 465 THR A 964 REMARK 465 LYS A 965 REMARK 465 PRO A 966 REMARK 465 PHE A 967 REMARK 465 SER A 968 REMARK 465 LEU A 969 REMARK 465 ASN A 970 REMARK 465 ILE A 971 REMARK 465 LYS A 972 REMARK 465 CYS A 973 REMARK 465 GLY A 974 REMARK 465 ASP A 975 REMARK 465 THR A 976 REMARK 465 SER A 977 REMARK 465 ILE A 978 REMARK 465 THR A 979 REMARK 465 PRO A 980 REMARK 465 LEU A 981 REMARK 465 VAL A 982 REMARK 465 ASP A 983 REMARK 465 TYR A 984 REMARK 465 ILE A 985 REMARK 465 PRO A 986 REMARK 465 GLN A 987 REMARK 465 ARG A 988 REMARK 465 LEU A 989 REMARK 465 ARG A 990 REMARK 465 TRP A 991 REMARK 465 MET A 992 REMARK 465 THR A 993 REMARK 465 GLU A 994 REMARK 465 TRP A 995 REMARK 465 ALA A 996 REMARK 465 GLU A 997 REMARK 465 TRP A 998 REMARK 465 TYR A 999 REMARK 465 CYS A 1000 REMARK 465 LYS A 1001 REMARK 465 GLU A 1002 REMARK 465 GLN A 1003 REMARK 465 SER A 1004 REMARK 465 ARG A 1005 REMARK 465 LEU A 1006 REMARK 465 TYR A 1007 REMARK 465 GLY A 1008 REMARK 465 GLU A 1009 REMARK 465 LEU A 1010 REMARK 465 VAL A 1011 REMARK 465 GLU A 1012 REMARK 465 LYS A 1013 REMARK 465 CYS A 1014 REMARK 465 ASN A 1015 REMARK 465 THR A 1016 REMARK 465 CYS A 1017 REMARK 465 GLY A 1018 REMARK 465 SER A 1019 REMARK 465 SER A 1020 REMARK 465 ASN A 1021 REMARK 465 GLY A 1022 REMARK 465 ILE A 1023 REMARK 465 VAL A 1024 REMARK 465 THR A 1025 REMARK 465 THR A 1026 REMARK 465 GLU A 1027 REMARK 465 ASP A 1028 REMARK 465 CYS A 1029 REMARK 465 LYS A 1030 REMARK 465 LYS A 1031 REMARK 465 LYS A 1032 REMARK 465 CYS A 1033 REMARK 465 MET A 1034 REMARK 465 GLN A 1035 REMARK 465 CYS A 1036 REMARK 465 LYS A 1037 REMARK 465 GLN A 1038 REMARK 465 LYS A 1039 REMARK 465 CYS A 1040 REMARK 465 GLU A 1041 REMARK 465 ALA A 1042 REMARK 465 TYR A 1043 REMARK 465 LYS A 1044 REMARK 465 SER A 1045 REMARK 465 PHE A 1046 REMARK 465 ILE A 1047 REMARK 465 GLU A 1048 REMARK 465 LYS A 1049 REMARK 465 TRP A 1050 REMARK 465 LYS A 1051 REMARK 465 LYS A 1052 REMARK 465 GLN A 1053 REMARK 465 TRP A 1054 REMARK 465 ASP A 1055 REMARK 465 GLU A 1056 REMARK 465 GLN A 1057 REMARK 465 GLU A 1058 REMARK 465 LYS A 1059 REMARK 465 LYS A 1060 REMARK 465 TYR A 1061 REMARK 465 GLN A 1062 REMARK 465 GLU A 1063 REMARK 465 LEU A 1064 REMARK 465 TYR A 1065 REMARK 465 ARG A 1066 REMARK 465 LYS A 1067 REMARK 465 ALA A 1068 REMARK 465 THR A 1069 REMARK 465 GLN A 1070 REMARK 465 ASN A 1071 REMARK 465 GLY A 1072 REMARK 465 SER A 1073 REMARK 465 ASP A 1074 REMARK 465 GLY A 1075 REMARK 465 SER A 1076 REMARK 465 LYS A 1077 REMARK 465 VAL A 1078 REMARK 465 THR A 1079 REMARK 465 ALA A 1080 REMARK 465 ASP A 1081 REMARK 465 LYS A 1082 REMARK 465 ASP A 1083 REMARK 465 ALA A 1084 REMARK 465 ASP A 1085 REMARK 465 VAL A 1086 REMARK 465 VAL A 1087 REMARK 465 ASP A 1088 REMARK 465 PHE A 1089 REMARK 465 LEU A 1090 REMARK 465 SER A 1091 REMARK 465 LYS A 1092 REMARK 465 LEU A 1093 REMARK 465 ARG A 1094 REMARK 465 ASN A 1095 REMARK 465 LYS A 1096 REMARK 465 ASN A 1097 REMARK 465 ASP A 1098 REMARK 465 THR A 1099 REMARK 465 ASN A 1100 REMARK 465 ASN A 1101 REMARK 465 LEU A 1102 REMARK 465 PHE A 1103 REMARK 465 GLU A 1104 REMARK 465 SER A 1105 REMARK 465 ALA A 1106 REMARK 465 ALA A 1107 REMARK 465 ALA A 1108 REMARK 465 TYR A 1109 REMARK 465 VAL A 1110 REMARK 465 HIS A 1111 REMARK 465 ASP A 1112 REMARK 465 THR A 1113 REMARK 465 GLY A 1114 REMARK 465 ASN A 1115 REMARK 465 LEU A 1116 REMARK 465 ASP A 1117 REMARK 465 ASP A 1118 REMARK 465 CYS A 1119 REMARK 465 ASN A 1120 REMARK 465 ALA A 1121 REMARK 465 GLN A 1122 REMARK 465 ASN A 1123 REMARK 465 ILE A 1124 REMARK 465 PHE A 1125 REMARK 465 CYS A 1126 REMARK 465 GLU A 1127 REMARK 465 LYS A 1128 REMARK 465 ASN A 1129 REMARK 465 CYS A 1130 REMARK 465 ASP A 1131 REMARK 465 GLY A 1132 REMARK 465 LYS A 1133 REMARK 465 VAL A 1134 REMARK 465 ASN A 1135 REMARK 465 ASP A 1136 REMARK 465 LYS A 1137 REMARK 465 TYR A 1138 REMARK 465 VAL A 1139 REMARK 465 PHE A 1140 REMARK 465 ARG A 1141 REMARK 465 LYS A 1142 REMARK 465 TYR A 1143 REMARK 465 PRO A 1144 REMARK 465 TYR A 1145 REMARK 465 ASP A 1146 REMARK 465 HIS A 1147 REMARK 465 ALA A 1148 REMARK 465 LYS A 1149 REMARK 465 ALA A 1150 REMARK 465 CYS A 1151 REMARK 465 ASN A 1152 REMARK 465 CYS A 1153 REMARK 465 ASN A 1154 REMARK 465 GLU A 1155 REMARK 465 ASN A 1156 REMARK 465 VAL A 1157 REMARK 465 THR A 1158 REMARK 465 PRO A 1159 REMARK 465 ARG A 1160 REMARK 465 PRO A 1161 REMARK 465 PRO A 1162 REMARK 465 ALA A 1163 REMARK 465 LEU A 1164 REMARK 465 SER A 1165 REMARK 465 ASN A 1166 REMARK 465 GLY A 1167 REMARK 465 SER A 1168 REMARK 465 GLY A 1169 REMARK 465 SER A 1170 REMARK 465 HIS A 1171 REMARK 465 HIS A 1172 REMARK 465 HIS A 1173 REMARK 465 HIS A 1174 REMARK 465 HIS A 1175 REMARK 465 HIS A 1176 REMARK 465 GLY A 1177 REMARK 465 SER A 1178 REMARK 465 GLY A 1179 REMARK 465 SER A 1180 REMARK 465 GLY A 1181 REMARK 465 LEU A 1182 REMARK 465 ASN A 1183 REMARK 465 ASP A 1184 REMARK 465 ILE A 1185 REMARK 465 PHE A 1186 REMARK 465 GLU A 1187 REMARK 465 ALA A 1188 REMARK 465 GLN A 1189 REMARK 465 LYS A 1190 REMARK 465 ILE A 1191 REMARK 465 GLU A 1192 REMARK 465 TRP A 1193 REMARK 465 HIS A 1194 REMARK 465 GLU A 1195 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT REMARK 500 REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT. REMARK 500 REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI DISTANCE REMARK 500 OD1 ASP L 82 OH TYR L 86 2.13 REMARK 500 OD2 ASP H 72 OG1 THR H 75 2.17 REMARK 500 REMARK 500 REMARK: NULL REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 SER L 67 57.99 -94.25 REMARK 500 ASN L 69 30.08 -98.87 REMARK 500 PRO L 80 -9.96 -58.78 REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: EMD-49201 RELATED DB: EMDB REMARK 900 CRYO-EM STRUCTURE OF 110_C4 FAB IN COMPLEX WITH CIDRA1.7 PFEMP1 DBREF 9NAQ H 1 113 PDB 9NAQ 9NAQ 1 113 DBREF 9NAQ L 1 107 PDB 9NAQ 9NAQ 1 107 DBREF 9NAQ A 1 1166 UNP A3R6S4 A3R6S4_PLAFA 1 1166 SEQADV 9NAQ GLN A 109 UNP A3R6S4 ARG 109 CONFLICT SEQADV 9NAQ GLU A 329 UNP A3R6S4 GLY 329 CONFLICT SEQADV 9NAQ GLY A 1167 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ SER A 1168 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ GLY A 1169 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ SER A 1170 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ HIS A 1171 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ HIS A 1172 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ HIS A 1173 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ HIS A 1174 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ HIS A 1175 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ HIS A 1176 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ GLY A 1177 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ SER A 1178 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ GLY A 1179 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ SER A 1180 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ GLY A 1181 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ LEU A 1182 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ ASN A 1183 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ ASP A 1184 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ ILE A 1185 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ PHE A 1186 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ GLU A 1187 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ ALA A 1188 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ GLN A 1189 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ LYS A 1190 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ ILE A 1191 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ GLU A 1192 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ TRP A 1193 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ HIS A 1194 UNP A3R6S4 EXPRESSION TAG SEQADV 9NAQ GLU A 1195 UNP A3R6S4 EXPRESSION TAG SEQRES 1 H 122 GLN VAL HIS LEU VAL GLN SER GLY ALA GLU VAL LYS LYS SEQRES 2 H 122 PRO GLY SER SER ALA THR VAL SER CYS LYS ALA SER GLY SEQRES 3 H 122 GLY PRO PHE ARG GLY TYR SER VAL SER TRP VAL ARG GLN SEQRES 4 H 122 ALA PRO GLY GLN GLY LEU GLU TRP MET GLY GLN ILE ILE SEQRES 5 H 122 THR MET LEU ASP THR ILE THR TYR ALA GLN ARG PHE GLN SEQRES 6 H 122 ASP ARG VAL THR ILE ARG ALA ASP GLU THR THR SER THR SEQRES 7 H 122 ILE TYR MET GLU LEU ASN SER LEU ARG SER GLU ASP THR SEQRES 8 H 122 ALA ILE TYR TYR CYS ALA ARG ASN GLY ASN ALA THR VAL SEQRES 9 H 122 GLY SER LEU GLY MET ASP VAL TRP GLY ARG GLY THR THR SEQRES 10 H 122 VAL THR VAL SER SER SEQRES 1 L 111 GLN ALA GLY LEU THR GLN PRO PRO SER GLU SER LYS GLY SEQRES 2 L 111 LEU ARG GLU THR ALA THR PHE THR CYS THR GLY ASN SER SEQRES 3 L 111 ASN ASN VAL GLY ASN GLN GLY ALA ALA TRP LEU GLN GLN SEQRES 4 L 111 HIS GLN GLY HIS PRO PRO LYS LEU LEU SER TYR ARG ASN SEQRES 5 L 111 ASN ASN ARG PRO SER GLY ILE SER GLU ARG PHE SER ALA SEQRES 6 L 111 SER ARG SER GLY ASN THR ALA SER LEU THR ILE THR GLY SEQRES 7 L 111 LEU GLN PRO GLU ASP GLU ALA VAL TYR PHE CYS SER ALA SEQRES 8 L 111 TRP ASP THR SER LEU ARG ALA TRP LEU PHE GLY GLY GLY SEQRES 9 L 111 THR HIS LEU THR VAL LEU GLY SEQRES 1 A 1195 MET GLY SER GLN SER SER LYS PRO SER LYS PRO SER VAL SEQRES 2 A 1195 ASP THR ASN GLU SER TYR LYS SER ALA ARG ASN VAL LEU SEQRES 3 A 1195 GLU ARG TYR ALA GLU SER ILE LYS GLN GLN ALA GLU ASN SEQRES 4 A 1195 ASP ALA SER GLY TYR GLU LYS GLU LEU LYS GLY LYS LEU SEQRES 5 A 1195 GLU GLU ALA SER PHE CYS GLY ALA TYR CYS GLU LEU ILE SEQRES 6 A 1195 GLY VAL PRO LYS TYR GLY SER THR ASP PRO CYS TYR LEU SEQRES 7 A 1195 ASP HIS ARG TRP HIS THR ASN LEU LEU HIS GLU LYS VAL SEQRES 8 A 1195 LYS ASP ARG ASP PRO CYS HIS ASN ARG ASN GLN LYS ARG SEQRES 9 A 1195 PHE ASP GLU GLY GLN VAL TYR GLU CYS GLY SER GLY ILE SEQRES 10 A 1195 ILE LYS GLY ASN GLY ASN ASN ARG ASN GLY GLY SER CYS SEQRES 11 A 1195 ALA PRO PRO ARG ARG ARG HIS MET CYS ASP LYS ASN LEU SEQRES 12 A 1195 GLU ALA LEU THR VAL ALA ASN THR LYS ASN SER ASN ASP SEQRES 13 A 1195 LEU LEU GLY ASN ILE LEU VAL THR ALA LYS TYR GLU GLY SEQRES 14 A 1195 ASP SER ILE VAL ASN SER TYR ALA ASN SER GLY MET PHE SEQRES 15 A 1195 ASN VAL CYS THR ALA LEU ALA ARG SER PHE ALA ASP ILE SEQRES 16 A 1195 GLY ASP ILE ILE ARG GLY LYS ASP LEU TYR LEU GLY ASN SEQRES 17 A 1195 GLY ASP TYR LYS GLU LYS VAL SER ASN ASN LEU ARG ALA SEQRES 18 A 1195 ILE PHE ASN LYS ILE TYR GLU ASN LEU ASN ASP PRO ASN SEQRES 19 A 1195 VAL LYS ALA HIS TYR GLN LYS ASP ALA PRO ASN TYR TYR SEQRES 20 A 1195 LYS LEU ARG GLU HIS TRP TRP THR VAL ASN ARG ASP GLN SEQRES 21 A 1195 VAL TRP LYS ALA ILE THR CYS ASN ALA PRO THR GLY ALA SEQRES 22 A 1195 ASP TYR PHE ARG LYS GLY SER ASP GLY THR ASN VAL PHE SEQRES 23 A 1195 THR SER GLN GLY GLN CYS GLY HIS TYR GLU GLY ALA PRO SEQRES 24 A 1195 PRO THR ASN LEU ASP TYR VAL PRO GLN PHE LEU ARG TRP SEQRES 25 A 1195 PHE GLU GLU TRP ALA GLU GLU PHE CYS ARG LYS LYS LYS SEQRES 26 A 1195 ILE LYS LEU GLU ASN VAL LYS LYS ALA CYS ARG ASP GLU SEQRES 27 A 1195 SER SER LYS LEU TYR CYS SER HIS ASN GLY TYR ASP CYS SEQRES 28 A 1195 THR GLN THR ILE ARG ASN LYS ASP ILE CYS ILE ARG GLU SEQRES 29 A 1195 SER LYS CYS THR ASP CYS SER THR LYS CYS LYS LEU TYR SEQRES 30 A 1195 GLU LEU TRP LEU GLU LYS GLN GLU ASN GLU PHE LYS LYS SEQRES 31 A 1195 GLN THR LYS LYS TYR ASP LYS GLU ILE ASN GLY ASN ASN SEQRES 32 A 1195 SER LEU GLN ASN ASN LYS ASN ASN GLY ILE ASP LYS LYS SEQRES 33 A 1195 TYR HIS ASN GLU PHE TYR LYS ASN PHE ARG GLU LYS GLY SEQRES 34 A 1195 TYR THR SER LEU ASP LYS PHE LEU LYS LEU LEU ASN GLU SEQRES 35 A 1195 GLY MET TYR CYS LYS ASN GLN LYS PRO GLU GLU GLU ASP SEQRES 36 A 1195 ILE ASP PHE THR LYS ASN GLY ASP LYS GLY ILE PHE TYR SEQRES 37 A 1195 ARG SER GLU TYR CYS GLN VAL CYS PRO TYR CYS GLY LEU SEQRES 38 A 1195 ASP CYS GLY GLY LYS THR CYS THR ALA LYS GLN GLU ILE SEQRES 39 A 1195 TYR PRO ASP CYS VAL TYR ASN GLY ALA TYR GLU PRO PRO SEQRES 40 A 1195 ASN GLY ALA GLU THR THR GLU ILE THR VAL LEU TYR SER SEQRES 41 A 1195 ALA ASP GLN GLU GLY ASP ILE SER ASN LYS LEU SER GLU SEQRES 42 A 1195 PHE CYS ASN ASP GLU ASN ASN LYS ASN SER GLN LYS TRP SEQRES 43 A 1195 GLN CYS TYR TYR VAL SER SER GLU ASN ASN GLY CYS LYS SEQRES 44 A 1195 MET GLU LYS LYS ASN ALA ASN HIS THR PRO GLU VAL LYS SEQRES 45 A 1195 ILE THR LYS PHE HIS ASN PHE PHE GLU MET TRP VAL THR SEQRES 46 A 1195 TYR LEU LEU THR GLU THR ILE THR TRP LYS ASP LYS LEU SEQRES 47 A 1195 LYS THR CYS MET ASN ASN THR LYS THR ALA ASP CYS ILE SEQRES 48 A 1195 HIS GLU CYS ASN LYS ASN CYS VAL CYS PHE ASP LYS TRP SEQRES 49 A 1195 VAL LYS GLN LYS GLU ASP GLU TRP ASN SER ILE LYS LYS SEQRES 50 A 1195 LEU PHE THR LYS GLU LYS LYS MET PRO LYS GLN TYR TYR SEQRES 51 A 1195 GLY ASN ILE ASN ILE TYR PHE GLU SER PHE PHE PHE HIS SEQRES 52 A 1195 VAL MET LYS LYS LEU ASN LYS GLU ALA LYS TRP ASN LYS SEQRES 53 A 1195 LEU MET ASP GLU LEU ARG ASN LYS ILE GLU LEU SER LYS SEQRES 54 A 1195 GLY ASN GLU GLY THR LYS ASP LEU GLN ASP ALA ILE GLU SEQRES 55 A 1195 LEU LEU LEU GLU TYR LEU LYS GLU LYS SER THR ILE CYS SEQRES 56 A 1195 LYS ASP ASN ASN THR ASN GLU ALA CYS ASP PRO THR VAL SEQRES 57 A 1195 ASP PRO THR LYS ASN PRO CYS GLY LYS ASN THR LYS ALA SEQRES 58 A 1195 GLY SER ASP LYS VAL ILE SER VAL LYS GLN ILE ALA GLN SEQRES 59 A 1195 TYR TYR LYS ARG LEU ALA HIS GLU GLN LEU GLU GLU ARG SEQRES 60 A 1195 GLY SER ARG SER ALA LEU LYS GLY ASP ALA SER LYS GLY SEQRES 61 A 1195 THR TYR ARG ARG GLN GLY ASN PRO ARG LYS LEU LYS LYS SEQRES 62 A 1195 VAL CYS ARG ILE ALA LYS ASP HIS SER ASN ARG ASN HIS SEQRES 63 A 1195 LYS ASP SER ARG GLY ARG HIS LEU CYS THR SER TYR LEU SEQRES 64 A 1195 GLU PHE LEU GLN THR ILE ASP ASP SER HIS ASN SER SER SEQRES 65 A 1195 ASN ALA LYS ARG VAL ASN ASN SER PHE LEU GLY ASP VAL SEQRES 66 A 1195 LEU LEU SER ALA LYS LEU ASP ALA ALA GLU ILE ILE LYS SEQRES 67 A 1195 ARG TYR LYS ASP GLN ASN ASN ILE ARG GLU ASN ILE GLU SEQRES 68 A 1195 GLN LYS ASP GLU GLU ALA MET CYS ARG ALA VAL ARG TYR SEQRES 69 A 1195 SER PHE ALA ASP LEU GLY ASP ILE ILE ARG GLY LYS ASP SEQRES 70 A 1195 LEU TRP ASP HIS LYS ASP PHE LYS LYS LEU GLU ARG ASP SEQRES 71 A 1195 LEU VAL LYS ILE PHE GLY LYS ILE LYS ASP GLU LEU LYS SEQRES 72 A 1195 SER LYS LEU GLY ASP LYS TYR ILE GLY ASP GLU ALA LYS SEQRES 73 A 1195 SER PRO TYR LYS GLN LEU ARG SER ASP TRP TRP GLU ALA SEQRES 74 A 1195 ASN ARG HIS GLN VAL TRP LYS ALA MET GLN CYS LYS THR SEQRES 75 A 1195 THR THR LYS PRO PHE SER LEU ASN ILE LYS CYS GLY ASP SEQRES 76 A 1195 THR SER ILE THR PRO LEU VAL ASP TYR ILE PRO GLN ARG SEQRES 77 A 1195 LEU ARG TRP MET THR GLU TRP ALA GLU TRP TYR CYS LYS SEQRES 78 A 1195 GLU GLN SER ARG LEU TYR GLY GLU LEU VAL GLU LYS CYS SEQRES 79 A 1195 ASN THR CYS GLY SER SER ASN GLY ILE VAL THR THR GLU SEQRES 80 A 1195 ASP CYS LYS LYS LYS CYS MET GLN CYS LYS GLN LYS CYS SEQRES 81 A 1195 GLU ALA TYR LYS SER PHE ILE GLU LYS TRP LYS LYS GLN SEQRES 82 A 1195 TRP ASP GLU GLN GLU LYS LYS TYR GLN GLU LEU TYR ARG SEQRES 83 A 1195 LYS ALA THR GLN ASN GLY SER ASP GLY SER LYS VAL THR SEQRES 84 A 1195 ALA ASP LYS ASP ALA ASP VAL VAL ASP PHE LEU SER LYS SEQRES 85 A 1195 LEU ARG ASN LYS ASN ASP THR ASN ASN LEU PHE GLU SER SEQRES 86 A 1195 ALA ALA ALA TYR VAL HIS ASP THR GLY ASN LEU ASP ASP SEQRES 87 A 1195 CYS ASN ALA GLN ASN ILE PHE CYS GLU LYS ASN CYS ASP SEQRES 88 A 1195 GLY LYS VAL ASN ASP LYS TYR VAL PHE ARG LYS TYR PRO SEQRES 89 A 1195 TYR ASP HIS ALA LYS ALA CYS ASN CYS ASN GLU ASN VAL SEQRES 90 A 1195 THR PRO ARG PRO PRO ALA LEU SER ASN GLY SER GLY SER SEQRES 91 A 1195 HIS HIS HIS HIS HIS HIS GLY SER GLY SER GLY LEU ASN SEQRES 92 A 1195 ASP ILE PHE GLU ALA GLN LYS ILE GLU TRP HIS GLU HELIX 1 AA1 PHE A 576 LEU A 598 1 23 HELIX 2 AA2 CYS A 620 LYS A 636 1 17 HELIX 3 AA3 ASN A 654 SER A 659 1 6 HELIX 4 AA4 PHE A 660 LEU A 668 1 9 HELIX 5 AA5 LYS A 670 MET A 678 1 9 HELIX 6 AA6 ALA A 700 ILE A 714 1 15 SHEET 1 AA1 4 HIS H 3 GLN H 6 0 SHEET 2 AA1 4 CYS H 22 SER H 25 -1 O SER H 25 N HIS H 3 SHEET 3 AA1 4 THR H 77 ILE H 78 -1 O ILE H 78 N CYS H 22 SHEET 4 AA1 4 ALA H 71 ASP H 72 -1 N ASP H 72 O THR H 77 SHEET 1 AA2 6 VAL H 11 LYS H 12 0 SHEET 2 AA2 6 THR H 107 VAL H 111 1 O THR H 110 N LYS H 12 SHEET 3 AA2 6 ALA H 88 ARG H 94 -1 N TYR H 90 O THR H 107 SHEET 4 AA2 6 VAL H 34 GLN H 39 -1 N VAL H 37 O TYR H 91 SHEET 5 AA2 6 LEU H 45 ILE H 52 -1 O GLY H 49 N TRP H 36 SHEET 6 AA2 6 THR H 56 TYR H 59 -1 O THR H 56 N ILE H 52 SHEET 1 AA3 2 GLY H 96 ASN H 97 0 SHEET 2 AA3 2 SER H 100B LEU H 100C-1 O SER H 100B N ASN H 97 SHEET 1 AA4 5 SER L 9 SER L 12 0 SHEET 2 AA4 5 THR L 102 THR L 105 1 O HIS L 103 N GLU L 11 SHEET 3 AA4 5 VAL L 85 ASP L 92 -1 N TYR L 86 O THR L 102 SHEET 4 AA4 5 ALA L 34 GLN L 38 -1 N ALA L 34 O SER L 89 SHEET 5 AA4 5 LYS L 45 SER L 48 -1 O LYS L 45 N GLN L 37 SHEET 1 AA5 4 SER L 9 SER L 12 0 SHEET 2 AA5 4 THR L 102 THR L 105 1 O HIS L 103 N GLU L 11 SHEET 3 AA5 4 VAL L 85 ASP L 92 -1 N TYR L 86 O THR L 102 SHEET 4 AA5 4 ALA L 95B PHE L 98 -1 O ALA L 95B N ASP L 92 SHEET 1 AA6 3 ALA L 19 THR L 20 0 SHEET 2 AA6 3 THR L 70 ILE L 75 -1 O ILE L 75 N ALA L 19 SHEET 3 AA6 3 CYS L 23 THR L 24 -1 N CYS L 23 O ALA L 71 SHEET 1 AA7 3 ALA L 19 THR L 20 0 SHEET 2 AA7 3 THR L 70 ILE L 75 -1 O ILE L 75 N ALA L 19 SHEET 3 AA7 3 PHE L 62 ARG L 66 -1 N SER L 63 O THR L 74 SSBOND 1 CYS H 22 CYS H 92 1555 1555 2.03 SSBOND 2 CYS L 23 CYS L 88 1555 1555 2.03 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000