HEADER MEMBRANE PROTEIN 10-MAR-25 9NOX TITLE TRANSMEMBRANE DOMAINS OF THE HUMAN TAS1R2 SWEET RECEPTOR SUBUNIT IN TITLE 2 COMPLEX WITH MINIGS/GUST25 COMPND MOL_ID: 1; COMPND 2 MOLECULE: MINIGS/GUST25; COMPND 3 CHAIN: B; COMPND 4 ENGINEERED: YES; COMPND 5 MOL_ID: 2; COMPND 6 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(T) SUBUNIT COMPND 7 BETA-1; COMPND 8 CHAIN: C; COMPND 9 SYNONYM: TRANSDUCIN BETA CHAIN 1; COMPND 10 ENGINEERED: YES; COMPND 11 MOL_ID: 3; COMPND 12 MOLECULE: GUANINE NUCLEOTIDE-BINDING PROTEIN G(I)/G(S)/G(O) SUBUNIT COMPND 13 GAMMA-2; COMPND 14 CHAIN: D; COMPND 15 SYNONYM: G GAMMA-I; COMPND 16 ENGINEERED: YES; COMPND 17 MOL_ID: 4; COMPND 18 MOLECULE: NANOBODY 35 (NB35); COMPND 19 CHAIN: E; COMPND 20 ENGINEERED: YES; COMPND 21 MOL_ID: 5; COMPND 22 MOLECULE: TASTE RECEPTOR TYPE 1 MEMBER 2; COMPND 23 CHAIN: A; COMPND 24 SYNONYM: G-PROTEIN COUPLED RECEPTOR 71,SWEET TASTE RECEPTOR T1R2; COMPND 25 ENGINEERED: YES SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 3 ORGANISM_TAXID: 9606; SOURCE 4 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 5 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 6 MOL_ID: 2; SOURCE 7 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 8 ORGANISM_COMMON: HUMAN; SOURCE 9 ORGANISM_TAXID: 9606; SOURCE 10 GENE: GNB1; SOURCE 11 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 12 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 13 MOL_ID: 3; SOURCE 14 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 15 ORGANISM_COMMON: HUMAN; SOURCE 16 ORGANISM_TAXID: 9606; SOURCE 17 GENE: GNG2; SOURCE 18 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 19 EXPRESSION_SYSTEM_TAXID: 9606; SOURCE 20 MOL_ID: 4; SOURCE 21 ORGANISM_SCIENTIFIC: LAMA GLAMA; SOURCE 22 ORGANISM_TAXID: 9844; SOURCE 23 EXPRESSION_SYSTEM: ESCHERICHIA COLI; SOURCE 24 EXPRESSION_SYSTEM_TAXID: 562; SOURCE 25 MOL_ID: 5; SOURCE 26 ORGANISM_SCIENTIFIC: HOMO SAPIENS; SOURCE 27 ORGANISM_COMMON: HUMAN; SOURCE 28 ORGANISM_TAXID: 9606; SOURCE 29 GENE: TAS1R2, GPR71, T1R2, TR2; SOURCE 30 EXPRESSION_SYSTEM: HOMO SAPIENS; SOURCE 31 EXPRESSION_SYSTEM_TAXID: 9606 KEYWDS SWEET, TASTE, RECEPTOR, GPCR, TAS1R2, TAS1R3, T1R2, T1R3, MEMBRANE KEYWDS 2 PROTEIN EXPDTA ELECTRON MICROSCOPY AUTHOR Z.JUEN,Z.LU,R.YU,A.N.CHANG,B.WANG,A.W.P.FITZPATRICK,C.S.ZUKER REVDAT 1 14-MAY-25 9NOX 0 JRNL AUTH Z.JUEN,Z.LU,R.YU,A.N.CHANG,B.WANG,A.W.FITZPATRICK,C.S.ZUKER JRNL TITL THE STRUCTURE OF HUMAN SWEETNESS JRNL REF CELL(CAMBRIDGE,MASS.) 2025 JRNL REFN ISSN 0092-8674 JRNL DOI 10.1016/J.CELL.2025.04.021 REMARK 2 REMARK 2 RESOLUTION. 3.00 ANGSTROMS. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 SOFTWARE PACKAGES : NULL REMARK 3 RECONSTRUCTION SCHEMA : NULL REMARK 3 REMARK 3 EM MAP-MODEL FITTING AND REFINEMENT REMARK 3 PDB ENTRY : NULL REMARK 3 REFINEMENT SPACE : NULL REMARK 3 REFINEMENT PROTOCOL : NULL REMARK 3 REFINEMENT TARGET : NULL REMARK 3 OVERALL ANISOTROPIC B VALUE : NULL REMARK 3 REMARK 3 FITTING PROCEDURE : NULL REMARK 3 REMARK 3 EM IMAGE RECONSTRUCTION STATISTICS REMARK 3 NOMINAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 ACTUAL PIXEL SIZE (ANGSTROMS) : NULL REMARK 3 EFFECTIVE RESOLUTION (ANGSTROMS) : 3.000 REMARK 3 NUMBER OF PARTICLES : 55925 REMARK 3 CTF CORRECTION METHOD : NONE REMARK 3 REMARK 3 EM RECONSTRUCTION MAGNIFICATION CALIBRATION: NULL REMARK 3 REMARK 3 OTHER DETAILS: NULL REMARK 4 REMARK 4 9NOX COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB ON 11-MAR-25. REMARK 100 THE DEPOSITION ID IS D_1000293567. REMARK 245 REMARK 245 EXPERIMENTAL DETAILS REMARK 245 RECONSTRUCTION METHOD : SINGLE PARTICLE REMARK 245 SPECIMEN TYPE : NULL REMARK 245 REMARK 245 ELECTRON MICROSCOPE SAMPLE REMARK 245 SAMPLE TYPE : PARTICLE REMARK 245 PARTICLE TYPE : POINT REMARK 245 NAME OF SAMPLE : TRANSMEMBRANE DOMAINS OF THE REMARK 245 HUMAN TAS1R2 SWEET RECEPTOR REMARK 245 SUBUNIT IN COMPLEX WITH MINIGS/ REMARK 245 GUST25 REMARK 245 SAMPLE CONCENTRATION (MG ML-1) : NULL REMARK 245 SAMPLE SUPPORT DETAILS : NULL REMARK 245 SAMPLE VITRIFICATION DETAILS : NULL REMARK 245 SAMPLE BUFFER : NULL REMARK 245 PH : 7.50 REMARK 245 SAMPLE DETAILS : NULL REMARK 245 REMARK 245 DATA ACQUISITION REMARK 245 DATE OF EXPERIMENT : NULL REMARK 245 NUMBER OF MICROGRAPHS-IMAGES : NULL REMARK 245 TEMPERATURE (KELVIN) : NULL REMARK 245 MICROSCOPE MODEL : TFS KRIOS REMARK 245 DETECTOR TYPE : GATAN K3 BIOQUANTUM (6K X REMARK 245 4K) REMARK 245 MINIMUM DEFOCUS (NM) : 1200.00 REMARK 245 MAXIMUM DEFOCUS (NM) : 2000.00 REMARK 245 MINIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 MAXIMUM TILT ANGLE (DEGREES) : NULL REMARK 245 NOMINAL CS : NULL REMARK 245 IMAGING MODE : BRIGHT FIELD REMARK 245 ELECTRON DOSE (ELECTRONS NM**-2) : 5900.00 REMARK 245 ILLUMINATION MODE : FLOOD BEAM REMARK 245 NOMINAL MAGNIFICATION : NULL REMARK 245 CALIBRATED MAGNIFICATION : NULL REMARK 245 SOURCE : FIELD EMISSION GUN REMARK 245 ACCELERATION VOLTAGE (KV) : 300 REMARK 245 IMAGING DETAILS : NULL REMARK 247 REMARK 247 ELECTRON MICROSCOPY REMARK 247 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM ELECTRON REMARK 247 MICROSCOPY DATA. PROTEIN DATA BANK CONVENTIONS REQUIRE REMARK 247 THAT CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES REMARK 247 ON THESE RECORDS ARE MEANINGLESS EXCEPT FOR THE CALCULATION REMARK 247 OF THE STRUCTURE FACTORS. REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON REMARK 300 BURIED SURFACE AREA. REMARK 350 REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: PENTAMERIC REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: PENTAMERIC REMARK 350 SOFTWARE USED: PISA REMARK 350 APPLY THE FOLLOWING TO CHAINS: B, C, D, E, A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 REMARK 465 M RES C SSSEQI REMARK 465 MET B -877 REMARK 465 LYS B -876 REMARK 465 THR B -875 REMARK 465 ILE B -874 REMARK 465 ILE B -873 REMARK 465 ALA B -872 REMARK 465 LEU B -871 REMARK 465 SER B -870 REMARK 465 TYR B -869 REMARK 465 ILE B -868 REMARK 465 PHE B -867 REMARK 465 CYS B -866 REMARK 465 LEU B -865 REMARK 465 VAL B -864 REMARK 465 PHE B -863 REMARK 465 ALA B -862 REMARK 465 ASP B -861 REMARK 465 TYR B -860 REMARK 465 LYS B -859 REMARK 465 ASP B -858 REMARK 465 ASP B -857 REMARK 465 ASP B -856 REMARK 465 ASP B -855 REMARK 465 LYS B -854 REMARK 465 ALA B -853 REMARK 465 ALA B -852 REMARK 465 ALA B -851 REMARK 465 ALA B -850 REMARK 465 PRO B -849 REMARK 465 LEU B -848 REMARK 465 CYS B -847 REMARK 465 LEU B -846 REMARK 465 SER B -845 REMARK 465 GLN B -844 REMARK 465 GLN B -843 REMARK 465 LEU B -842 REMARK 465 ARG B -841 REMARK 465 MET B -840 REMARK 465 LYS B -839 REMARK 465 GLY B -838 REMARK 465 ASP B -837 REMARK 465 TYR B -836 REMARK 465 VAL B -835 REMARK 465 LEU B -834 REMARK 465 GLY B -833 REMARK 465 GLY B -832 REMARK 465 LEU B -831 REMARK 465 PHE B -830 REMARK 465 PRO B -829 REMARK 465 LEU B -828 REMARK 465 GLY B -827 REMARK 465 GLU B -826 REMARK 465 ALA B -825 REMARK 465 GLU B -824 REMARK 465 GLU B -823 REMARK 465 ALA B -822 REMARK 465 GLY B -821 REMARK 465 LEU B -820 REMARK 465 ARG B -819 REMARK 465 SER B -818 REMARK 465 ARG B -817 REMARK 465 THR B -816 REMARK 465 ARG B -815 REMARK 465 PRO B -814 REMARK 465 SER B -813 REMARK 465 SER B -812 REMARK 465 PRO B -811 REMARK 465 VAL B -810 REMARK 465 CYS B -809 REMARK 465 THR B -808 REMARK 465 ARG B -807 REMARK 465 PHE B -806 REMARK 465 SER B -805 REMARK 465 SER B -804 REMARK 465 ASN B -803 REMARK 465 GLY B -802 REMARK 465 LEU B -801 REMARK 465 LEU B -800 REMARK 465 TRP B -799 REMARK 465 ALA B -798 REMARK 465 LEU B -797 REMARK 465 ALA B -796 REMARK 465 MET B -795 REMARK 465 LYS B -794 REMARK 465 MET B -793 REMARK 465 ALA B -792 REMARK 465 VAL B -791 REMARK 465 GLU B -790 REMARK 465 GLU B -789 REMARK 465 ILE B -788 REMARK 465 ASN B -787 REMARK 465 ASN B -786 REMARK 465 LYS B -785 REMARK 465 SER B -784 REMARK 465 ASP B -783 REMARK 465 LEU B -782 REMARK 465 LEU B -781 REMARK 465 PRO B -780 REMARK 465 GLY B -779 REMARK 465 LEU B -778 REMARK 465 ARG B -777 REMARK 465 LEU B -776 REMARK 465 GLY B -775 REMARK 465 TYR B -774 REMARK 465 ASP B -773 REMARK 465 LEU B -772 REMARK 465 PHE B -771 REMARK 465 ASP B -770 REMARK 465 THR B -769 REMARK 465 CYS B -768 REMARK 465 SER B -767 REMARK 465 GLU B -766 REMARK 465 PRO B -765 REMARK 465 VAL B -764 REMARK 465 VAL B -763 REMARK 465 ALA B -762 REMARK 465 MET B -761 REMARK 465 LYS B -760 REMARK 465 PRO B -759 REMARK 465 SER B -758 REMARK 465 LEU B -757 REMARK 465 MET B -756 REMARK 465 PHE B -755 REMARK 465 LEU B -754 REMARK 465 ALA B -753 REMARK 465 LYS B -752 REMARK 465 ALA B -751 REMARK 465 GLY B -750 REMARK 465 SER B -749 REMARK 465 ARG B -748 REMARK 465 ASP B -747 REMARK 465 ILE B -746 REMARK 465 ALA B -745 REMARK 465 ALA B -744 REMARK 465 TYR B -743 REMARK 465 CYS B -742 REMARK 465 ASN B -741 REMARK 465 TYR B -740 REMARK 465 THR B -739 REMARK 465 GLN B -738 REMARK 465 TYR B -737 REMARK 465 GLN B -736 REMARK 465 PRO B -735 REMARK 465 ARG B -734 REMARK 465 VAL B -733 REMARK 465 LEU B -732 REMARK 465 ALA B -731 REMARK 465 VAL B -730 REMARK 465 ILE B -729 REMARK 465 GLY B -728 REMARK 465 PRO B -727 REMARK 465 HIS B -726 REMARK 465 SER B -725 REMARK 465 SER B -724 REMARK 465 GLU B -723 REMARK 465 LEU B -722 REMARK 465 ALA B -721 REMARK 465 MET B -720 REMARK 465 VAL B -719 REMARK 465 THR B -718 REMARK 465 GLY B -717 REMARK 465 LYS B -716 REMARK 465 PHE B -715 REMARK 465 PHE B -714 REMARK 465 SER B -713 REMARK 465 PHE B -712 REMARK 465 PHE B -711 REMARK 465 LEU B -710 REMARK 465 MET B -709 REMARK 465 PRO B -708 REMARK 465 GLN B -707 REMARK 465 VAL B -706 REMARK 465 SER B -705 REMARK 465 TYR B -704 REMARK 465 GLY B -703 REMARK 465 ALA B -702 REMARK 465 SER B -701 REMARK 465 MET B -700 REMARK 465 GLU B -699 REMARK 465 LEU B -698 REMARK 465 LEU B -697 REMARK 465 SER B -696 REMARK 465 ALA B -695 REMARK 465 ARG B -694 REMARK 465 GLU B -693 REMARK 465 THR B -692 REMARK 465 PHE B -691 REMARK 465 PRO B -690 REMARK 465 SER B -689 REMARK 465 PHE B -688 REMARK 465 PHE B -687 REMARK 465 ARG B -686 REMARK 465 THR B -685 REMARK 465 VAL B -684 REMARK 465 PRO B -683 REMARK 465 SER B -682 REMARK 465 ASP B -681 REMARK 465 ARG B -680 REMARK 465 VAL B -679 REMARK 465 GLN B -678 REMARK 465 LEU B -677 REMARK 465 THR B -676 REMARK 465 ALA B -675 REMARK 465 ALA B -674 REMARK 465 ALA B -673 REMARK 465 GLU B -672 REMARK 465 LEU B -671 REMARK 465 LEU B -670 REMARK 465 GLN B -669 REMARK 465 GLU B -668 REMARK 465 PHE B -667 REMARK 465 GLY B -666 REMARK 465 TRP B -665 REMARK 465 ASN B -664 REMARK 465 TRP B -663 REMARK 465 VAL B -662 REMARK 465 ALA B -661 REMARK 465 ALA B -660 REMARK 465 LEU B -659 REMARK 465 GLY B -658 REMARK 465 SER B -657 REMARK 465 ASP B -656 REMARK 465 ASP B -655 REMARK 465 GLU B -654 REMARK 465 TYR B -653 REMARK 465 GLY B -652 REMARK 465 ARG B -651 REMARK 465 GLN B -650 REMARK 465 GLY B -649 REMARK 465 LEU B -648 REMARK 465 SER B -647 REMARK 465 ILE B -646 REMARK 465 PHE B -645 REMARK 465 SER B -644 REMARK 465 ALA B -643 REMARK 465 LEU B -642 REMARK 465 ALA B -641 REMARK 465 ALA B -640 REMARK 465 ALA B -639 REMARK 465 ARG B -638 REMARK 465 GLY B -637 REMARK 465 ILE B -636 REMARK 465 CYS B -635 REMARK 465 ILE B -634 REMARK 465 ALA B -633 REMARK 465 HIS B -632 REMARK 465 GLU B -631 REMARK 465 GLY B -630 REMARK 465 LEU B -629 REMARK 465 VAL B -628 REMARK 465 PRO B -627 REMARK 465 LEU B -626 REMARK 465 PRO B -625 REMARK 465 ARG B -624 REMARK 465 ALA B -623 REMARK 465 ASP B -622 REMARK 465 ASP B -621 REMARK 465 SER B -620 REMARK 465 ARG B -619 REMARK 465 LEU B -618 REMARK 465 GLY B -617 REMARK 465 LYS B -616 REMARK 465 VAL B -615 REMARK 465 GLN B -614 REMARK 465 ASP B -613 REMARK 465 VAL B -612 REMARK 465 LEU B -611 REMARK 465 HIS B -610 REMARK 465 GLN B -609 REMARK 465 VAL B -608 REMARK 465 ASN B -607 REMARK 465 GLN B -606 REMARK 465 SER B -605 REMARK 465 SER B -604 REMARK 465 VAL B -603 REMARK 465 GLN B -602 REMARK 465 VAL B -601 REMARK 465 VAL B -600 REMARK 465 LEU B -599 REMARK 465 LEU B -598 REMARK 465 PHE B -597 REMARK 465 ALA B -596 REMARK 465 SER B -595 REMARK 465 VAL B -594 REMARK 465 HIS B -593 REMARK 465 ALA B -592 REMARK 465 ALA B -591 REMARK 465 HIS B -590 REMARK 465 ALA B -589 REMARK 465 LEU B -588 REMARK 465 PHE B -587 REMARK 465 ASN B -586 REMARK 465 TYR B -585 REMARK 465 SER B -584 REMARK 465 ILE B -583 REMARK 465 SER B -582 REMARK 465 SER B -581 REMARK 465 ARG B -580 REMARK 465 LEU B -579 REMARK 465 SER B -578 REMARK 465 PRO B -577 REMARK 465 LYS B -576 REMARK 465 VAL B -575 REMARK 465 TRP B -574 REMARK 465 VAL B -573 REMARK 465 ALA B -572 REMARK 465 SER B -571 REMARK 465 GLU B -570 REMARK 465 ALA B -569 REMARK 465 TRP B -568 REMARK 465 LEU B -567 REMARK 465 THR B -566 REMARK 465 SER B -565 REMARK 465 ASP B -564 REMARK 465 LEU B -563 REMARK 465 VAL B -562 REMARK 465 MET B -561 REMARK 465 GLY B -560 REMARK 465 LEU B -559 REMARK 465 PRO B -558 REMARK 465 GLY B -557 REMARK 465 MET B -556 REMARK 465 ALA B -555 REMARK 465 GLN B -554 REMARK 465 MET B -553 REMARK 465 GLY B -552 REMARK 465 THR B -551 REMARK 465 VAL B -550 REMARK 465 LEU B -549 REMARK 465 GLY B -548 REMARK 465 PHE B -547 REMARK 465 LEU B -546 REMARK 465 GLN B -545 REMARK 465 ARG B -544 REMARK 465 GLY B -543 REMARK 465 ALA B -542 REMARK 465 GLN B -541 REMARK 465 LEU B -540 REMARK 465 HIS B -539 REMARK 465 GLU B -538 REMARK 465 PHE B -537 REMARK 465 PRO B -536 REMARK 465 GLN B -535 REMARK 465 TYR B -534 REMARK 465 VAL B -533 REMARK 465 LYS B -532 REMARK 465 THR B -531 REMARK 465 HIS B -530 REMARK 465 LEU B -529 REMARK 465 ALA B -528 REMARK 465 LEU B -527 REMARK 465 ALA B -526 REMARK 465 THR B -525 REMARK 465 ASP B -524 REMARK 465 PRO B -523 REMARK 465 ALA B -522 REMARK 465 PHE B -521 REMARK 465 CYS B -520 REMARK 465 SER B -519 REMARK 465 ALA B -518 REMARK 465 LEU B -517 REMARK 465 GLY B -516 REMARK 465 GLU B -515 REMARK 465 ARG B -514 REMARK 465 GLU B -513 REMARK 465 GLN B -512 REMARK 465 GLY B -511 REMARK 465 LEU B -510 REMARK 465 GLU B -509 REMARK 465 GLU B -508 REMARK 465 ASP B -507 REMARK 465 VAL B -506 REMARK 465 VAL B -505 REMARK 465 GLY B -504 REMARK 465 GLN B -503 REMARK 465 ARG B -502 REMARK 465 CYS B -501 REMARK 465 PRO B -500 REMARK 465 GLN B -499 REMARK 465 CYS B -498 REMARK 465 ASP B -497 REMARK 465 CYS B -496 REMARK 465 ILE B -495 REMARK 465 THR B -494 REMARK 465 LEU B -493 REMARK 465 GLN B -492 REMARK 465 ASN B -491 REMARK 465 VAL B -490 REMARK 465 SER B -489 REMARK 465 ALA B -488 REMARK 465 GLY B -487 REMARK 465 LEU B -486 REMARK 465 ASN B -485 REMARK 465 HIS B -484 REMARK 465 HIS B -483 REMARK 465 GLN B -482 REMARK 465 THR B -481 REMARK 465 PHE B -480 REMARK 465 SER B -479 REMARK 465 VAL B -478 REMARK 465 TYR B -477 REMARK 465 ALA B -476 REMARK 465 ALA B -475 REMARK 465 VAL B -474 REMARK 465 TYR B -473 REMARK 465 SER B -472 REMARK 465 VAL B -471 REMARK 465 ALA B -470 REMARK 465 GLN B -469 REMARK 465 ALA B -468 REMARK 465 LEU B -467 REMARK 465 HIS B -466 REMARK 465 ASN B -465 REMARK 465 THR B -464 REMARK 465 LEU B -463 REMARK 465 GLN B -462 REMARK 465 CYS B -461 REMARK 465 ASN B -460 REMARK 465 ALA B -459 REMARK 465 SER B -458 REMARK 465 GLY B -457 REMARK 465 CYS B -456 REMARK 465 PRO B -455 REMARK 465 ALA B -454 REMARK 465 GLN B -453 REMARK 465 ASP B -452 REMARK 465 PRO B -451 REMARK 465 VAL B -450 REMARK 465 LYS B -449 REMARK 465 PRO B -448 REMARK 465 TRP B -447 REMARK 465 GLN B -446 REMARK 465 LEU B -445 REMARK 465 LEU B -444 REMARK 465 GLU B -443 REMARK 465 ASN B -442 REMARK 465 MET B -441 REMARK 465 TYR B -440 REMARK 465 ASN B -439 REMARK 465 LEU B -438 REMARK 465 THR B -437 REMARK 465 PHE B -436 REMARK 465 HIS B -435 REMARK 465 VAL B -434 REMARK 465 GLY B -433 REMARK 465 GLY B -432 REMARK 465 LEU B -431 REMARK 465 PRO B -430 REMARK 465 LEU B -429 REMARK 465 ARG B -428 REMARK 465 PHE B -427 REMARK 465 ASP B -426 REMARK 465 SER B -425 REMARK 465 SER B -424 REMARK 465 GLY B -423 REMARK 465 ASN B -422 REMARK 465 VAL B -421 REMARK 465 ASP B -420 REMARK 465 MET B -419 REMARK 465 GLU B -418 REMARK 465 TYR B -417 REMARK 465 ASP B -416 REMARK 465 LEU B -415 REMARK 465 LYS B -414 REMARK 465 LEU B -413 REMARK 465 TRP B -412 REMARK 465 VAL B -411 REMARK 465 TRP B -410 REMARK 465 GLN B -409 REMARK 465 GLY B -408 REMARK 465 SER B -407 REMARK 465 VAL B -406 REMARK 465 PRO B -405 REMARK 465 ARG B -404 REMARK 465 LEU B -403 REMARK 465 HIS B -402 REMARK 465 ASP B -401 REMARK 465 VAL B -400 REMARK 465 GLY B -399 REMARK 465 ARG B -398 REMARK 465 PHE B -397 REMARK 465 ASN B -396 REMARK 465 GLY B -395 REMARK 465 SER B -394 REMARK 465 LEU B -393 REMARK 465 ARG B -392 REMARK 465 THR B -391 REMARK 465 GLU B -390 REMARK 465 ARG B -389 REMARK 465 LEU B -388 REMARK 465 LYS B -387 REMARK 465 ILE B -386 REMARK 465 ARG B -385 REMARK 465 TRP B -384 REMARK 465 HIS B -383 REMARK 465 THR B -382 REMARK 465 SER B -381 REMARK 465 ASP B -380 REMARK 465 ASN B -379 REMARK 465 GLN B -378 REMARK 465 LYS B -377 REMARK 465 PRO B -376 REMARK 465 VAL B -375 REMARK 465 SER B -374 REMARK 465 ARG B -373 REMARK 465 CYS B -372 REMARK 465 SER B -371 REMARK 465 ARG B -370 REMARK 465 GLN B -369 REMARK 465 CYS B -368 REMARK 465 GLN B -367 REMARK 465 GLU B -366 REMARK 465 GLY B -365 REMARK 465 GLN B -364 REMARK 465 VAL B -363 REMARK 465 ARG B -362 REMARK 465 ARG B -361 REMARK 465 VAL B -360 REMARK 465 LYS B -359 REMARK 465 GLY B -358 REMARK 465 PHE B -357 REMARK 465 HIS B -356 REMARK 465 SER B -355 REMARK 465 CYS B -354 REMARK 465 CYS B -353 REMARK 465 TYR B -352 REMARK 465 ASP B -351 REMARK 465 CYS B -350 REMARK 465 VAL B -349 REMARK 465 ASP B -348 REMARK 465 CYS B -347 REMARK 465 GLU B -346 REMARK 465 ALA B -345 REMARK 465 GLY B -344 REMARK 465 SER B -343 REMARK 465 TYR B -342 REMARK 465 ARG B -341 REMARK 465 GLN B -340 REMARK 465 ASN B -339 REMARK 465 PRO B -338 REMARK 465 ASP B -337 REMARK 465 ASP B -336 REMARK 465 ILE B -335 REMARK 465 ALA B -334 REMARK 465 CYS B -333 REMARK 465 THR B -332 REMARK 465 PHE B -331 REMARK 465 CYS B -330 REMARK 465 GLY B -329 REMARK 465 GLN B -328 REMARK 465 ASP B -327 REMARK 465 GLU B -326 REMARK 465 TRP B -325 REMARK 465 SER B -324 REMARK 465 PRO B -323 REMARK 465 GLU B -322 REMARK 465 ARG B -321 REMARK 465 SER B -320 REMARK 465 THR B -319 REMARK 465 ARG B -318 REMARK 465 CYS B -317 REMARK 465 PHE B -316 REMARK 465 ARG B -315 REMARK 465 ARG B -314 REMARK 465 ARG B -313 REMARK 465 SER B -312 REMARK 465 ARG B -311 REMARK 465 PHE B -310 REMARK 465 LEU B -309 REMARK 465 ALA B -308 REMARK 465 TRP B -307 REMARK 465 GLY B -306 REMARK 465 GLU B -305 REMARK 465 PRO B -304 REMARK 465 ALA B -303 REMARK 465 VAL B -302 REMARK 465 LEU B -301 REMARK 465 LEU B -300 REMARK 465 LEU B -299 REMARK 465 LEU B -298 REMARK 465 LEU B -297 REMARK 465 LEU B -296 REMARK 465 LEU B -295 REMARK 465 SER B -294 REMARK 465 LEU B -293 REMARK 465 ALA B -292 REMARK 465 LEU B -291 REMARK 465 GLY B -290 REMARK 465 LEU B -289 REMARK 465 VAL B -288 REMARK 465 LEU B -287 REMARK 465 ALA B -286 REMARK 465 ALA B -285 REMARK 465 LEU B -284 REMARK 465 GLY B -283 REMARK 465 LEU B -282 REMARK 465 PHE B -281 REMARK 465 VAL B -280 REMARK 465 HIS B -279 REMARK 465 HIS B -278 REMARK 465 ARG B -277 REMARK 465 ASP B -276 REMARK 465 SER B -275 REMARK 465 PRO B -274 REMARK 465 LEU B -273 REMARK 465 VAL B -272 REMARK 465 GLN B -271 REMARK 465 ALA B -270 REMARK 465 SER B -269 REMARK 465 GLY B -268 REMARK 465 GLY B -267 REMARK 465 PRO B -266 REMARK 465 LEU B -265 REMARK 465 ALA B -264 REMARK 465 CYS B -263 REMARK 465 PHE B -262 REMARK 465 GLY B -261 REMARK 465 LEU B -260 REMARK 465 VAL B -259 REMARK 465 CYS B -258 REMARK 465 LEU B -257 REMARK 465 GLY B -256 REMARK 465 LEU B -255 REMARK 465 VAL B -254 REMARK 465 CYS B -253 REMARK 465 LEU B -252 REMARK 465 SER B -251 REMARK 465 VAL B -250 REMARK 465 LEU B -249 REMARK 465 LEU B -248 REMARK 465 PHE B -247 REMARK 465 PRO B -246 REMARK 465 GLY B -245 REMARK 465 GLN B -244 REMARK 465 PRO B -243 REMARK 465 SER B -242 REMARK 465 PRO B -241 REMARK 465 ALA B -240 REMARK 465 ARG B -239 REMARK 465 CYS B -238 REMARK 465 LEU B -237 REMARK 465 ALA B -236 REMARK 465 GLN B -235 REMARK 465 GLN B -234 REMARK 465 PRO B -233 REMARK 465 LEU B -232 REMARK 465 SER B -231 REMARK 465 HIS B -230 REMARK 465 LEU B -229 REMARK 465 PRO B -228 REMARK 465 LEU B -227 REMARK 465 THR B -226 REMARK 465 GLY B -225 REMARK 465 CYS B -224 REMARK 465 LEU B -223 REMARK 465 SER B -222 REMARK 465 THR B -221 REMARK 465 LEU B -220 REMARK 465 PHE B -219 REMARK 465 LEU B -218 REMARK 465 GLN B -217 REMARK 465 ALA B -216 REMARK 465 ALA B -215 REMARK 465 GLU B -214 REMARK 465 ILE B -213 REMARK 465 PHE B -212 REMARK 465 VAL B -211 REMARK 465 GLU B -210 REMARK 465 SER B -209 REMARK 465 GLU B -208 REMARK 465 LEU B -207 REMARK 465 PRO B -206 REMARK 465 LEU B -205 REMARK 465 SER B -204 REMARK 465 TRP B -203 REMARK 465 ALA B -202 REMARK 465 ASP B -201 REMARK 465 ARG B -200 REMARK 465 LEU B -199 REMARK 465 SER B -198 REMARK 465 GLY B -197 REMARK 465 CYS B -196 REMARK 465 LEU B -195 REMARK 465 ARG B -194 REMARK 465 GLY B -193 REMARK 465 PRO B -192 REMARK 465 TRP B -191 REMARK 465 ALA B -190 REMARK 465 TRP B -189 REMARK 465 LEU B -188 REMARK 465 VAL B -187 REMARK 465 VAL B -186 REMARK 465 LEU B -185 REMARK 465 LEU B -184 REMARK 465 ALA B -183 REMARK 465 MET B -182 REMARK 465 LEU B -181 REMARK 465 VAL B -180 REMARK 465 GLU B -179 REMARK 465 VAL B -178 REMARK 465 ALA B -177 REMARK 465 LEU B -176 REMARK 465 CYS B -175 REMARK 465 THR B -174 REMARK 465 TRP B -173 REMARK 465 TYR B -172 REMARK 465 LEU B -171 REMARK 465 VAL B -170 REMARK 465 ALA B -169 REMARK 465 PHE B -168 REMARK 465 PRO B -167 REMARK 465 PRO B -166 REMARK 465 GLU B -165 REMARK 465 VAL B -164 REMARK 465 VAL B -163 REMARK 465 THR B -162 REMARK 465 ASP B -161 REMARK 465 TRP B -160 REMARK 465 HIS B -159 REMARK 465 MET B -158 REMARK 465 LEU B -157 REMARK 465 PRO B -156 REMARK 465 THR B -155 REMARK 465 GLU B -154 REMARK 465 ALA B -153 REMARK 465 LEU B -152 REMARK 465 VAL B -151 REMARK 465 HIS B -150 REMARK 465 CYS B -149 REMARK 465 ARG B -148 REMARK 465 THR B -147 REMARK 465 ARG B -146 REMARK 465 SER B -145 REMARK 465 TRP B -144 REMARK 465 VAL B -143 REMARK 465 SER B -142 REMARK 465 PHE B -141 REMARK 465 GLY B -140 REMARK 465 LEU B -139 REMARK 465 ALA B -138 REMARK 465 HIS B -137 REMARK 465 ALA B -136 REMARK 465 THR B -135 REMARK 465 ASN B -134 REMARK 465 ALA B -133 REMARK 465 THR B -132 REMARK 465 LEU B -131 REMARK 465 ALA B -130 REMARK 465 PHE B -129 REMARK 465 LEU B -128 REMARK 465 CYS B -127 REMARK 465 PHE B -126 REMARK 465 LEU B -125 REMARK 465 GLY B -124 REMARK 465 THR B -123 REMARK 465 PHE B -122 REMARK 465 LEU B -121 REMARK 465 VAL B -120 REMARK 465 ARG B -119 REMARK 465 SER B -118 REMARK 465 GLN B -117 REMARK 465 PRO B -116 REMARK 465 GLY B -115 REMARK 465 ARG B -114 REMARK 465 TYR B -113 REMARK 465 ASN B -112 REMARK 465 ARG B -111 REMARK 465 ALA B -110 REMARK 465 ARG B -109 REMARK 465 GLY B -108 REMARK 465 LEU B -107 REMARK 465 THR B -106 REMARK 465 PHE B -105 REMARK 465 ALA B -104 REMARK 465 MET B -103 REMARK 465 LEU B -102 REMARK 465 ALA B -101 REMARK 465 TYR B -100 REMARK 465 PHE B -99 REMARK 465 ILE B -98 REMARK 465 THR B -97 REMARK 465 TRP B -96 REMARK 465 VAL B -95 REMARK 465 SER B -94 REMARK 465 PHE B -93 REMARK 465 VAL B -92 REMARK 465 PRO B -91 REMARK 465 LEU B -90 REMARK 465 LEU B -89 REMARK 465 ALA B -88 REMARK 465 ASN B -87 REMARK 465 VAL B -86 REMARK 465 GLN B -85 REMARK 465 VAL B -84 REMARK 465 VAL B -83 REMARK 465 LEU B -82 REMARK 465 ARG B -81 REMARK 465 PRO B -80 REMARK 465 ALA B -79 REMARK 465 VAL B -78 REMARK 465 GLN B -77 REMARK 465 MET B -76 REMARK 465 GLY B -75 REMARK 465 ALA B -74 REMARK 465 LEU B -73 REMARK 465 LEU B -72 REMARK 465 LEU B -71 REMARK 465 CYS B -70 REMARK 465 VAL B -69 REMARK 465 LEU B -68 REMARK 465 GLY B -67 REMARK 465 ILE B -66 REMARK 465 LEU B -65 REMARK 465 ALA B -64 REMARK 465 ALA B -63 REMARK 465 PHE B -62 REMARK 465 HIS B -61 REMARK 465 LEU B -60 REMARK 465 PRO B -59 REMARK 465 ARG B -58 REMARK 465 CYS B -57 REMARK 465 TYR B -56 REMARK 465 LEU B -55 REMARK 465 LEU B -54 REMARK 465 MET B -53 REMARK 465 ARG B -52 REMARK 465 GLN B -51 REMARK 465 PRO B -50 REMARK 465 GLY B -49 REMARK 465 LEU B -48 REMARK 465 ASN B -47 REMARK 465 THR B -46 REMARK 465 PRO B -45 REMARK 465 GLU B -44 REMARK 465 PHE B -43 REMARK 465 PHE B -42 REMARK 465 LEU B -41 REMARK 465 GLY B -40 REMARK 465 GLY B -39 REMARK 465 GLY B -38 REMARK 465 PRO B -37 REMARK 465 GLY B -36 REMARK 465 ASP B -35 REMARK 465 ALA B -34 REMARK 465 GLN B -33 REMARK 465 GLY B -32 REMARK 465 GLN B -31 REMARK 465 ASN B -30 REMARK 465 ASP B -29 REMARK 465 GLY B -28 REMARK 465 ASN B -27 REMARK 465 THR B -26 REMARK 465 GLY B -25 REMARK 465 ASN B -24 REMARK 465 GLN B -23 REMARK 465 GLY B -22 REMARK 465 LYS B -21 REMARK 465 HIS B -20 REMARK 465 GLU B -19 REMARK 465 GLY B -18 REMARK 465 GLY B -17 REMARK 465 GLY B -16 REMARK 465 SER B -15 REMARK 465 GLY B -14 REMARK 465 GLY B -13 REMARK 465 GLY B -12 REMARK 465 SER B -11 REMARK 465 GLY B -10 REMARK 465 GLY B -9 REMARK 465 GLY B -8 REMARK 465 SER B -7 REMARK 465 GLY B -6 REMARK 465 GLY B -5 REMARK 465 GLY B -4 REMARK 465 SER B -3 REMARK 465 GLY B -2 REMARK 465 GLY B -1 REMARK 465 GLY B 0 REMARK 465 SER B 1 REMARK 465 GLY B 2 REMARK 465 ASN B 3 REMARK 465 SER B 4 REMARK 465 LYS B 5 REMARK 465 THR B 6 REMARK 465 GLY B 65 REMARK 465 GLY B 66 REMARK 465 SER B 67 REMARK 465 GLY B 68 REMARK 465 GLY B 69 REMARK 465 THR B 70 REMARK 465 MET C 1 REMARK 465 MET D 1 REMARK 465 ALA D 2 REMARK 465 SER D 3 REMARK 465 ASN D 4 REMARK 465 ASN D 5 REMARK 465 THR D 6 REMARK 465 PRO D 60 REMARK 465 PHE D 61 REMARK 465 ARG D 62 REMARK 465 GLU D 63 REMARK 465 LYS D 64 REMARK 465 LYS D 65 REMARK 465 PHE D 66 REMARK 465 PHE D 67 REMARK 465 CYS D 68 REMARK 465 ALA D 69 REMARK 465 ILE D 70 REMARK 465 LEU D 71 REMARK 465 MET E -21 REMARK 465 LYS E -20 REMARK 465 TYR E -19 REMARK 465 LEU E -18 REMARK 465 LEU E -17 REMARK 465 PRO E -16 REMARK 465 THR E -15 REMARK 465 ALA E -14 REMARK 465 ALA E -13 REMARK 465 ALA E -12 REMARK 465 GLY E -11 REMARK 465 LEU E -10 REMARK 465 LEU E -9 REMARK 465 LEU E -8 REMARK 465 LEU E -7 REMARK 465 ALA E -6 REMARK 465 ALA E -5 REMARK 465 GLN E -4 REMARK 465 PRO E -3 REMARK 465 ALA E -2 REMARK 465 MET E -1 REMARK 465 ALA E 0 REMARK 465 HIS E 129 REMARK 465 HIS E 130 REMARK 465 HIS E 131 REMARK 465 HIS E 132 REMARK 465 HIS E 133 REMARK 465 HIS E 134 REMARK 465 GLU E 135 REMARK 465 PRO E 136 REMARK 465 GLU E 137 REMARK 465 ALA E 138 REMARK 465 MET A -8 REMARK 465 LYS A -7 REMARK 465 THR A -6 REMARK 465 ILE A -5 REMARK 465 ILE A -4 REMARK 465 ALA A -3 REMARK 465 LEU A -2 REMARK 465 SER A -1 REMARK 465 TYR A 0 REMARK 465 ILE A 1 REMARK 465 PHE A 2 REMARK 465 CYS A 3 REMARK 465 LEU A 4 REMARK 465 VAL A 5 REMARK 465 PHE A 6 REMARK 465 ALA A 7 REMARK 465 TYR A 8 REMARK 465 PRO A 9 REMARK 465 TYR A 10 REMARK 465 ASP A 11 REMARK 465 VAL A 12 REMARK 465 PRO A 13 REMARK 465 ASP A 14 REMARK 465 TYR A 15 REMARK 465 ALA A 16 REMARK 465 ALA A 17 REMARK 465 ALA A 18 REMARK 465 ALA A 19 REMARK 465 GLU A 20 REMARK 465 PRO A 21 REMARK 465 ALA A 22 REMARK 465 GLU A 23 REMARK 465 ASN A 24 REMARK 465 SER A 25 REMARK 465 ASP A 26 REMARK 465 PHE A 27 REMARK 465 TYR A 28 REMARK 465 LEU A 29 REMARK 465 PRO A 30 REMARK 465 GLY A 31 REMARK 465 ASP A 32 REMARK 465 TYR A 33 REMARK 465 LEU A 34 REMARK 465 LEU A 35 REMARK 465 GLY A 36 REMARK 465 GLY A 37 REMARK 465 LEU A 38 REMARK 465 PHE A 39 REMARK 465 SER A 40 REMARK 465 LEU A 41 REMARK 465 HIS A 42 REMARK 465 ALA A 43 REMARK 465 ASN A 44 REMARK 465 MET A 45 REMARK 465 LYS A 46 REMARK 465 GLY A 47 REMARK 465 ILE A 48 REMARK 465 VAL A 49 REMARK 465 HIS A 50 REMARK 465 LEU A 51 REMARK 465 ASN A 52 REMARK 465 PHE A 53 REMARK 465 LEU A 54 REMARK 465 GLN A 55 REMARK 465 VAL A 56 REMARK 465 PRO A 57 REMARK 465 MET A 58 REMARK 465 CYS A 59 REMARK 465 LYS A 60 REMARK 465 GLU A 61 REMARK 465 TYR A 62 REMARK 465 GLU A 63 REMARK 465 VAL A 64 REMARK 465 LYS A 65 REMARK 465 VAL A 66 REMARK 465 ILE A 67 REMARK 465 GLY A 68 REMARK 465 TYR A 69 REMARK 465 ASN A 70 REMARK 465 LEU A 71 REMARK 465 MET A 72 REMARK 465 GLN A 73 REMARK 465 ALA A 74 REMARK 465 MET A 75 REMARK 465 ARG A 76 REMARK 465 PHE A 77 REMARK 465 ALA A 78 REMARK 465 VAL A 79 REMARK 465 GLU A 80 REMARK 465 GLU A 81 REMARK 465 ILE A 82 REMARK 465 ASN A 83 REMARK 465 ASN A 84 REMARK 465 ASP A 85 REMARK 465 SER A 86 REMARK 465 SER A 87 REMARK 465 LEU A 88 REMARK 465 LEU A 89 REMARK 465 PRO A 90 REMARK 465 GLY A 91 REMARK 465 VAL A 92 REMARK 465 LEU A 93 REMARK 465 LEU A 94 REMARK 465 GLY A 95 REMARK 465 TYR A 96 REMARK 465 GLU A 97 REMARK 465 ILE A 98 REMARK 465 VAL A 99 REMARK 465 ASP A 100 REMARK 465 VAL A 101 REMARK 465 CYS A 102 REMARK 465 TYR A 103 REMARK 465 ILE A 104 REMARK 465 SER A 105 REMARK 465 ASN A 106 REMARK 465 ASN A 107 REMARK 465 VAL A 108 REMARK 465 GLN A 109 REMARK 465 PRO A 110 REMARK 465 VAL A 111 REMARK 465 LEU A 112 REMARK 465 TYR A 113 REMARK 465 PHE A 114 REMARK 465 LEU A 115 REMARK 465 ALA A 116 REMARK 465 HIS A 117 REMARK 465 GLU A 118 REMARK 465 ASP A 119 REMARK 465 ASN A 120 REMARK 465 LEU A 121 REMARK 465 LEU A 122 REMARK 465 PRO A 123 REMARK 465 ILE A 124 REMARK 465 GLN A 125 REMARK 465 GLU A 126 REMARK 465 ASP A 127 REMARK 465 TYR A 128 REMARK 465 SER A 129 REMARK 465 ASN A 130 REMARK 465 TYR A 131 REMARK 465 ILE A 132 REMARK 465 SER A 133 REMARK 465 ARG A 134 REMARK 465 VAL A 135 REMARK 465 VAL A 136 REMARK 465 ALA A 137 REMARK 465 VAL A 138 REMARK 465 ILE A 139 REMARK 465 GLY A 140 REMARK 465 PRO A 141 REMARK 465 ASP A 142 REMARK 465 ASN A 143 REMARK 465 SER A 144 REMARK 465 GLU A 145 REMARK 465 SER A 146 REMARK 465 VAL A 147 REMARK 465 MET A 148 REMARK 465 THR A 149 REMARK 465 VAL A 150 REMARK 465 ALA A 151 REMARK 465 ASN A 152 REMARK 465 PHE A 153 REMARK 465 LEU A 154 REMARK 465 SER A 155 REMARK 465 LEU A 156 REMARK 465 PHE A 157 REMARK 465 LEU A 158 REMARK 465 LEU A 159 REMARK 465 PRO A 160 REMARK 465 GLN A 161 REMARK 465 ILE A 162 REMARK 465 THR A 163 REMARK 465 TYR A 164 REMARK 465 SER A 165 REMARK 465 ALA A 166 REMARK 465 ILE A 167 REMARK 465 SER A 168 REMARK 465 ASP A 169 REMARK 465 GLU A 170 REMARK 465 LEU A 171 REMARK 465 ARG A 172 REMARK 465 ASP A 173 REMARK 465 LYS A 174 REMARK 465 VAL A 175 REMARK 465 ARG A 176 REMARK 465 PHE A 177 REMARK 465 PRO A 178 REMARK 465 ALA A 179 REMARK 465 LEU A 180 REMARK 465 LEU A 181 REMARK 465 ARG A 182 REMARK 465 THR A 183 REMARK 465 THR A 184 REMARK 465 PRO A 185 REMARK 465 SER A 186 REMARK 465 ALA A 187 REMARK 465 ASP A 188 REMARK 465 HIS A 189 REMARK 465 HIS A 190 REMARK 465 ILE A 191 REMARK 465 GLU A 192 REMARK 465 ALA A 193 REMARK 465 MET A 194 REMARK 465 VAL A 195 REMARK 465 GLN A 196 REMARK 465 LEU A 197 REMARK 465 MET A 198 REMARK 465 LEU A 199 REMARK 465 HIS A 200 REMARK 465 PHE A 201 REMARK 465 ARG A 202 REMARK 465 TRP A 203 REMARK 465 ASN A 204 REMARK 465 TRP A 205 REMARK 465 ILE A 206 REMARK 465 ILE A 207 REMARK 465 VAL A 208 REMARK 465 LEU A 209 REMARK 465 VAL A 210 REMARK 465 SER A 211 REMARK 465 SER A 212 REMARK 465 ASP A 213 REMARK 465 THR A 214 REMARK 465 TYR A 215 REMARK 465 GLY A 216 REMARK 465 ARG A 217 REMARK 465 ASP A 218 REMARK 465 ASN A 219 REMARK 465 GLY A 220 REMARK 465 GLN A 221 REMARK 465 LEU A 222 REMARK 465 LEU A 223 REMARK 465 GLY A 224 REMARK 465 GLU A 225 REMARK 465 ARG A 226 REMARK 465 VAL A 227 REMARK 465 ALA A 228 REMARK 465 ARG A 229 REMARK 465 ARG A 230 REMARK 465 ASP A 231 REMARK 465 ILE A 232 REMARK 465 CYS A 233 REMARK 465 ILE A 234 REMARK 465 ALA A 235 REMARK 465 PHE A 236 REMARK 465 GLN A 237 REMARK 465 GLU A 238 REMARK 465 THR A 239 REMARK 465 LEU A 240 REMARK 465 PRO A 241 REMARK 465 THR A 242 REMARK 465 LEU A 243 REMARK 465 GLN A 244 REMARK 465 PRO A 245 REMARK 465 ASN A 246 REMARK 465 GLN A 247 REMARK 465 ASN A 248 REMARK 465 MET A 249 REMARK 465 THR A 250 REMARK 465 SER A 251 REMARK 465 GLU A 252 REMARK 465 GLU A 253 REMARK 465 ARG A 254 REMARK 465 GLN A 255 REMARK 465 ARG A 256 REMARK 465 LEU A 257 REMARK 465 VAL A 258 REMARK 465 THR A 259 REMARK 465 ILE A 260 REMARK 465 VAL A 261 REMARK 465 ASP A 262 REMARK 465 LYS A 263 REMARK 465 LEU A 264 REMARK 465 GLN A 265 REMARK 465 GLN A 266 REMARK 465 SER A 267 REMARK 465 THR A 268 REMARK 465 ALA A 269 REMARK 465 ARG A 270 REMARK 465 VAL A 271 REMARK 465 VAL A 272 REMARK 465 VAL A 273 REMARK 465 VAL A 274 REMARK 465 PHE A 275 REMARK 465 SER A 276 REMARK 465 PRO A 277 REMARK 465 ASP A 278 REMARK 465 LEU A 279 REMARK 465 THR A 280 REMARK 465 LEU A 281 REMARK 465 TYR A 282 REMARK 465 HIS A 283 REMARK 465 PHE A 284 REMARK 465 PHE A 285 REMARK 465 ASN A 286 REMARK 465 GLU A 287 REMARK 465 VAL A 288 REMARK 465 LEU A 289 REMARK 465 ARG A 290 REMARK 465 GLN A 291 REMARK 465 ASN A 292 REMARK 465 PHE A 293 REMARK 465 THR A 294 REMARK 465 GLY A 295 REMARK 465 ALA A 296 REMARK 465 VAL A 297 REMARK 465 TRP A 298 REMARK 465 ILE A 299 REMARK 465 ALA A 300 REMARK 465 SER A 301 REMARK 465 GLU A 302 REMARK 465 SER A 303 REMARK 465 TRP A 304 REMARK 465 ALA A 305 REMARK 465 ILE A 306 REMARK 465 ASP A 307 REMARK 465 PRO A 308 REMARK 465 VAL A 309 REMARK 465 LEU A 310 REMARK 465 HIS A 311 REMARK 465 ASN A 312 REMARK 465 LEU A 313 REMARK 465 THR A 314 REMARK 465 GLU A 315 REMARK 465 LEU A 316 REMARK 465 ARG A 317 REMARK 465 HIS A 318 REMARK 465 LEU A 319 REMARK 465 GLY A 320 REMARK 465 THR A 321 REMARK 465 PHE A 322 REMARK 465 LEU A 323 REMARK 465 GLY A 324 REMARK 465 ILE A 325 REMARK 465 THR A 326 REMARK 465 ILE A 327 REMARK 465 GLN A 328 REMARK 465 SER A 329 REMARK 465 VAL A 330 REMARK 465 PRO A 331 REMARK 465 ILE A 332 REMARK 465 PRO A 333 REMARK 465 GLY A 334 REMARK 465 PHE A 335 REMARK 465 SER A 336 REMARK 465 GLU A 337 REMARK 465 PHE A 338 REMARK 465 ARG A 339 REMARK 465 GLU A 340 REMARK 465 TRP A 341 REMARK 465 GLY A 342 REMARK 465 PRO A 343 REMARK 465 GLN A 344 REMARK 465 ALA A 345 REMARK 465 GLY A 346 REMARK 465 PRO A 347 REMARK 465 PRO A 348 REMARK 465 PRO A 349 REMARK 465 LEU A 350 REMARK 465 SER A 351 REMARK 465 ARG A 352 REMARK 465 THR A 353 REMARK 465 SER A 354 REMARK 465 GLN A 355 REMARK 465 SER A 356 REMARK 465 TYR A 357 REMARK 465 THR A 358 REMARK 465 CYS A 359 REMARK 465 ASN A 360 REMARK 465 GLN A 361 REMARK 465 GLU A 362 REMARK 465 CYS A 363 REMARK 465 ASP A 364 REMARK 465 ASN A 365 REMARK 465 CYS A 366 REMARK 465 LEU A 367 REMARK 465 ASN A 368 REMARK 465 ALA A 369 REMARK 465 THR A 370 REMARK 465 LEU A 371 REMARK 465 SER A 372 REMARK 465 PHE A 373 REMARK 465 ASN A 374 REMARK 465 THR A 375 REMARK 465 ILE A 376 REMARK 465 LEU A 377 REMARK 465 ARG A 378 REMARK 465 LEU A 379 REMARK 465 SER A 380 REMARK 465 GLY A 381 REMARK 465 GLU A 382 REMARK 465 ARG A 383 REMARK 465 VAL A 384 REMARK 465 VAL A 385 REMARK 465 TYR A 386 REMARK 465 SER A 387 REMARK 465 VAL A 388 REMARK 465 TYR A 389 REMARK 465 SER A 390 REMARK 465 ALA A 391 REMARK 465 VAL A 392 REMARK 465 TYR A 393 REMARK 465 ALA A 394 REMARK 465 VAL A 395 REMARK 465 ALA A 396 REMARK 465 HIS A 397 REMARK 465 ALA A 398 REMARK 465 LEU A 399 REMARK 465 HIS A 400 REMARK 465 SER A 401 REMARK 465 LEU A 402 REMARK 465 LEU A 403 REMARK 465 GLY A 404 REMARK 465 CYS A 405 REMARK 465 ASP A 406 REMARK 465 LYS A 407 REMARK 465 SER A 408 REMARK 465 THR A 409 REMARK 465 CYS A 410 REMARK 465 THR A 411 REMARK 465 LYS A 412 REMARK 465 ARG A 413 REMARK 465 VAL A 414 REMARK 465 VAL A 415 REMARK 465 TYR A 416 REMARK 465 PRO A 417 REMARK 465 TRP A 418 REMARK 465 GLN A 419 REMARK 465 LEU A 420 REMARK 465 LEU A 421 REMARK 465 GLU A 422 REMARK 465 GLU A 423 REMARK 465 ILE A 424 REMARK 465 TRP A 425 REMARK 465 LYS A 426 REMARK 465 VAL A 427 REMARK 465 ASN A 428 REMARK 465 PHE A 429 REMARK 465 THR A 430 REMARK 465 LEU A 431 REMARK 465 LEU A 432 REMARK 465 ASP A 433 REMARK 465 HIS A 434 REMARK 465 GLN A 435 REMARK 465 ILE A 436 REMARK 465 PHE A 437 REMARK 465 PHE A 438 REMARK 465 ASP A 439 REMARK 465 PRO A 440 REMARK 465 GLN A 441 REMARK 465 GLY A 442 REMARK 465 ASP A 443 REMARK 465 VAL A 444 REMARK 465 ALA A 445 REMARK 465 LEU A 446 REMARK 465 HIS A 447 REMARK 465 LEU A 448 REMARK 465 GLU A 449 REMARK 465 ILE A 450 REMARK 465 VAL A 451 REMARK 465 GLN A 452 REMARK 465 TRP A 453 REMARK 465 GLN A 454 REMARK 465 TRP A 455 REMARK 465 ASP A 456 REMARK 465 ARG A 457 REMARK 465 SER A 458 REMARK 465 GLN A 459 REMARK 465 ASN A 460 REMARK 465 PRO A 461 REMARK 465 PHE A 462 REMARK 465 GLN A 463 REMARK 465 SER A 464 REMARK 465 VAL A 465 REMARK 465 ALA A 466 REMARK 465 SER A 467 REMARK 465 TYR A 468 REMARK 465 TYR A 469 REMARK 465 PRO A 470 REMARK 465 LEU A 471 REMARK 465 GLN A 472 REMARK 465 ARG A 473 REMARK 465 GLN A 474 REMARK 465 LEU A 475 REMARK 465 LYS A 476 REMARK 465 ASN A 477 REMARK 465 ILE A 478 REMARK 465 GLN A 479 REMARK 465 ASP A 480 REMARK 465 ILE A 481 REMARK 465 SER A 482 REMARK 465 TRP A 483 REMARK 465 HIS A 484 REMARK 465 THR A 485 REMARK 465 ILE A 486 REMARK 465 ASN A 487 REMARK 465 ASN A 488 REMARK 465 THR A 489 REMARK 465 ILE A 490 REMARK 465 PRO A 491 REMARK 465 MET A 492 REMARK 465 SER A 493 REMARK 465 MET A 494 REMARK 465 CYS A 495 REMARK 465 SER A 496 REMARK 465 LYS A 497 REMARK 465 ARG A 498 REMARK 465 CYS A 499 REMARK 465 GLN A 500 REMARK 465 SER A 501 REMARK 465 GLY A 502 REMARK 465 GLN A 503 REMARK 465 LYS A 504 REMARK 465 LYS A 505 REMARK 465 LYS A 506 REMARK 465 PRO A 507 REMARK 465 VAL A 508 REMARK 465 GLY A 509 REMARK 465 ILE A 510 REMARK 465 HIS A 511 REMARK 465 VAL A 512 REMARK 465 CYS A 513 REMARK 465 CYS A 514 REMARK 465 PHE A 515 REMARK 465 GLU A 516 REMARK 465 CYS A 517 REMARK 465 ILE A 518 REMARK 465 ASP A 519 REMARK 465 CYS A 520 REMARK 465 LEU A 521 REMARK 465 PRO A 522 REMARK 465 GLY A 523 REMARK 465 THR A 524 REMARK 465 PHE A 525 REMARK 465 LEU A 526 REMARK 465 ASN A 527 REMARK 465 HIS A 528 REMARK 465 THR A 529 REMARK 465 GLU A 530 REMARK 465 ASP A 531 REMARK 465 GLU A 532 REMARK 465 TYR A 533 REMARK 465 GLU A 534 REMARK 465 CYS A 535 REMARK 465 GLN A 536 REMARK 465 ALA A 537 REMARK 465 CYS A 538 REMARK 465 PRO A 539 REMARK 465 ASN A 540 REMARK 465 ASN A 541 REMARK 465 GLU A 542 REMARK 465 TRP A 543 REMARK 465 SER A 544 REMARK 465 TYR A 545 REMARK 465 GLN A 546 REMARK 465 SER A 547 REMARK 465 GLU A 548 REMARK 465 THR A 549 REMARK 465 SER A 550 REMARK 465 CYS A 551 REMARK 465 PHE A 552 REMARK 465 LYS A 553 REMARK 465 ARG A 554 REMARK 465 GLN A 555 REMARK 465 LEU A 556 REMARK 465 VAL A 557 REMARK 465 PHE A 558 REMARK 465 THR A 700 REMARK 465 GLY A 701 REMARK 465 LEU A 702 REMARK 465 SER A 703 REMARK 465 PRO A 704 REMARK 465 THR A 705 REMARK 465 THR A 706 REMARK 465 ARG A 707 REMARK 465 THR A 708 REMARK 465 ASP A 709 REMARK 465 PRO A 710 REMARK 465 ASP A 711 REMARK 465 ASP A 712 REMARK 465 PRO A 713 REMARK 465 LYS A 714 REMARK 465 ILE A 715 REMARK 465 THR A 716 REMARK 465 ILE A 717 REMARK 465 VAL A 718 REMARK 465 SER A 719 REMARK 465 CYS A 720 REMARK 465 ASN A 721 REMARK 465 PRO A 722 REMARK 465 ASN A 723 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2) REMARK 500 REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI- REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400 REMARK 500 REMARK 500 M RES CSSEQI PSI PHI REMARK 500 PHE B 104 52.48 -91.32 REMARK 500 LEU B 138 49.83 -93.32 REMARK 500 ASP B 179 46.90 -84.84 REMARK 500 ALA B 222 -129.08 55.52 REMARK 500 THR C 196 -9.06 73.47 REMARK 500 VAL E 48 -55.81 -120.95 REMARK 500 GLN A 592 29.19 46.65 REMARK 500 REMARK 500 REMARK: NULL REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: EMD-49620 RELATED DB: EMDB REMARK 900 TRANSMEMBRANE DOMAINS OF THE HUMAN TAS1R2 SWEET RECEPTOR SUBUNIT IN REMARK 900 COMPLEX WITH MINIGS/GUST25 DBREF 9NOX B -877 250 PDB 9NOX 9NOX -877 250 DBREF 9NOX C 1 340 UNP P62873 GBB1_HUMAN 1 340 DBREF 9NOX D 1 71 UNP P59768 GBG2_HUMAN 1 71 DBREF 9NOX E -21 138 PDB 9NOX 9NOX -21 138 DBREF 9NOX A 19 839 UNP Q8TE23 TS1R2_HUMAN 19 839 SEQADV 9NOX MET A -8 UNP Q8TE23 INITIATING METHIONINE SEQADV 9NOX LYS A -7 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX THR A -6 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ILE A -5 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ILE A -4 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ALA A -3 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX LEU A -2 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX SER A -1 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX TYR A 0 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ILE A 1 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX PHE A 2 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX CYS A 3 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX LEU A 4 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX VAL A 5 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX PHE A 6 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ALA A 7 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX TYR A 8 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX PRO A 9 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX TYR A 10 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ASP A 11 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX VAL A 12 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX PRO A 13 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ASP A 14 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX TYR A 15 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ALA A 16 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ALA A 17 UNP Q8TE23 EXPRESSION TAG SEQADV 9NOX ALA A 18 UNP Q8TE23 EXPRESSION TAG SEQRES 1 B 1128 MET LYS THR ILE ILE ALA LEU SER TYR ILE PHE CYS LEU SEQRES 2 B 1128 VAL PHE ALA ASP TYR LYS ASP ASP ASP ASP LYS ALA ALA SEQRES 3 B 1128 ALA ALA PRO LEU CYS LEU SER GLN GLN LEU ARG MET LYS SEQRES 4 B 1128 GLY ASP TYR VAL LEU GLY GLY LEU PHE PRO LEU GLY GLU SEQRES 5 B 1128 ALA GLU GLU ALA GLY LEU ARG SER ARG THR ARG PRO SER SEQRES 6 B 1128 SER PRO VAL CYS THR ARG PHE SER SER ASN GLY LEU LEU SEQRES 7 B 1128 TRP ALA LEU ALA MET LYS MET ALA VAL GLU GLU ILE ASN SEQRES 8 B 1128 ASN LYS SER ASP LEU LEU PRO GLY LEU ARG LEU GLY TYR SEQRES 9 B 1128 ASP LEU PHE ASP THR CYS SER GLU PRO VAL VAL ALA MET SEQRES 10 B 1128 LYS PRO SER LEU MET PHE LEU ALA LYS ALA GLY SER ARG SEQRES 11 B 1128 ASP ILE ALA ALA TYR CYS ASN TYR THR GLN TYR GLN PRO SEQRES 12 B 1128 ARG VAL LEU ALA VAL ILE GLY PRO HIS SER SER GLU LEU SEQRES 13 B 1128 ALA MET VAL THR GLY LYS PHE PHE SER PHE PHE LEU MET SEQRES 14 B 1128 PRO GLN VAL SER TYR GLY ALA SER MET GLU LEU LEU SER SEQRES 15 B 1128 ALA ARG GLU THR PHE PRO SER PHE PHE ARG THR VAL PRO SEQRES 16 B 1128 SER ASP ARG VAL GLN LEU THR ALA ALA ALA GLU LEU LEU SEQRES 17 B 1128 GLN GLU PHE GLY TRP ASN TRP VAL ALA ALA LEU GLY SER SEQRES 18 B 1128 ASP ASP GLU TYR GLY ARG GLN GLY LEU SER ILE PHE SER SEQRES 19 B 1128 ALA LEU ALA ALA ALA ARG GLY ILE CYS ILE ALA HIS GLU SEQRES 20 B 1128 GLY LEU VAL PRO LEU PRO ARG ALA ASP ASP SER ARG LEU SEQRES 21 B 1128 GLY LYS VAL GLN ASP VAL LEU HIS GLN VAL ASN GLN SER SEQRES 22 B 1128 SER VAL GLN VAL VAL LEU LEU PHE ALA SER VAL HIS ALA SEQRES 23 B 1128 ALA HIS ALA LEU PHE ASN TYR SER ILE SER SER ARG LEU SEQRES 24 B 1128 SER PRO LYS VAL TRP VAL ALA SER GLU ALA TRP LEU THR SEQRES 25 B 1128 SER ASP LEU VAL MET GLY LEU PRO GLY MET ALA GLN MET SEQRES 26 B 1128 GLY THR VAL LEU GLY PHE LEU GLN ARG GLY ALA GLN LEU SEQRES 27 B 1128 HIS GLU PHE PRO GLN TYR VAL LYS THR HIS LEU ALA LEU SEQRES 28 B 1128 ALA THR ASP PRO ALA PHE CYS SER ALA LEU GLY GLU ARG SEQRES 29 B 1128 GLU GLN GLY LEU GLU GLU ASP VAL VAL GLY GLN ARG CYS SEQRES 30 B 1128 PRO GLN CYS ASP CYS ILE THR LEU GLN ASN VAL SER ALA SEQRES 31 B 1128 GLY LEU ASN HIS HIS GLN THR PHE SER VAL TYR ALA ALA SEQRES 32 B 1128 VAL TYR SER VAL ALA GLN ALA LEU HIS ASN THR LEU GLN SEQRES 33 B 1128 CYS ASN ALA SER GLY CYS PRO ALA GLN ASP PRO VAL LYS SEQRES 34 B 1128 PRO TRP GLN LEU LEU GLU ASN MET TYR ASN LEU THR PHE SEQRES 35 B 1128 HIS VAL GLY GLY LEU PRO LEU ARG PHE ASP SER SER GLY SEQRES 36 B 1128 ASN VAL ASP MET GLU TYR ASP LEU LYS LEU TRP VAL TRP SEQRES 37 B 1128 GLN GLY SER VAL PRO ARG LEU HIS ASP VAL GLY ARG PHE SEQRES 38 B 1128 ASN GLY SER LEU ARG THR GLU ARG LEU LYS ILE ARG TRP SEQRES 39 B 1128 HIS THR SER ASP ASN GLN LYS PRO VAL SER ARG CYS SER SEQRES 40 B 1128 ARG GLN CYS GLN GLU GLY GLN VAL ARG ARG VAL LYS GLY SEQRES 41 B 1128 PHE HIS SER CYS CYS TYR ASP CYS VAL ASP CYS GLU ALA SEQRES 42 B 1128 GLY SER TYR ARG GLN ASN PRO ASP ASP ILE ALA CYS THR SEQRES 43 B 1128 PHE CYS GLY GLN ASP GLU TRP SER PRO GLU ARG SER THR SEQRES 44 B 1128 ARG CYS PHE ARG ARG ARG SER ARG PHE LEU ALA TRP GLY SEQRES 45 B 1128 GLU PRO ALA VAL LEU LEU LEU LEU LEU LEU LEU SER LEU SEQRES 46 B 1128 ALA LEU GLY LEU VAL LEU ALA ALA LEU GLY LEU PHE VAL SEQRES 47 B 1128 HIS HIS ARG ASP SER PRO LEU VAL GLN ALA SER GLY GLY SEQRES 48 B 1128 PRO LEU ALA CYS PHE GLY LEU VAL CYS LEU GLY LEU VAL SEQRES 49 B 1128 CYS LEU SER VAL LEU LEU PHE PRO GLY GLN PRO SER PRO SEQRES 50 B 1128 ALA ARG CYS LEU ALA GLN GLN PRO LEU SER HIS LEU PRO SEQRES 51 B 1128 LEU THR GLY CYS LEU SER THR LEU PHE LEU GLN ALA ALA SEQRES 52 B 1128 GLU ILE PHE VAL GLU SER GLU LEU PRO LEU SER TRP ALA SEQRES 53 B 1128 ASP ARG LEU SER GLY CYS LEU ARG GLY PRO TRP ALA TRP SEQRES 54 B 1128 LEU VAL VAL LEU LEU ALA MET LEU VAL GLU VAL ALA LEU SEQRES 55 B 1128 CYS THR TRP TYR LEU VAL ALA PHE PRO PRO GLU VAL VAL SEQRES 56 B 1128 THR ASP TRP HIS MET LEU PRO THR GLU ALA LEU VAL HIS SEQRES 57 B 1128 CYS ARG THR ARG SER TRP VAL SER PHE GLY LEU ALA HIS SEQRES 58 B 1128 ALA THR ASN ALA THR LEU ALA PHE LEU CYS PHE LEU GLY SEQRES 59 B 1128 THR PHE LEU VAL ARG SER GLN PRO GLY ARG TYR ASN ARG SEQRES 60 B 1128 ALA ARG GLY LEU THR PHE ALA MET LEU ALA TYR PHE ILE SEQRES 61 B 1128 THR TRP VAL SER PHE VAL PRO LEU LEU ALA ASN VAL GLN SEQRES 62 B 1128 VAL VAL LEU ARG PRO ALA VAL GLN MET GLY ALA LEU LEU SEQRES 63 B 1128 LEU CYS VAL LEU GLY ILE LEU ALA ALA PHE HIS LEU PRO SEQRES 64 B 1128 ARG CYS TYR LEU LEU MET ARG GLN PRO GLY LEU ASN THR SEQRES 65 B 1128 PRO GLU PHE PHE LEU GLY GLY GLY PRO GLY ASP ALA GLN SEQRES 66 B 1128 GLY GLN ASN ASP GLY ASN THR GLY ASN GLN GLY LYS HIS SEQRES 67 B 1128 GLU GLY GLY GLY SER GLY GLY GLY SER GLY GLY GLY SER SEQRES 68 B 1128 GLY GLY GLY SER GLY GLY GLY SER GLY ASN SER LYS THR SEQRES 69 B 1128 GLU ASP GLN ARG ASN GLU GLU LYS ALA GLN ARG GLU ALA SEQRES 70 B 1128 ASN LYS LYS ILE GLU LYS GLN LEU GLN LYS ASP LYS GLN SEQRES 71 B 1128 VAL TYR ARG ALA THR HIS ARG LEU LEU LEU LEU GLY ALA SEQRES 72 B 1128 ASP ASN SER GLY LYS SER THR ILE VAL LYS GLN MET ARG SEQRES 73 B 1128 ILE LEU HIS GLY GLY SER GLY GLY SER GLY GLY THR SER SEQRES 74 B 1128 GLY ILE PHE GLU THR LYS PHE GLN VAL ASP LYS VAL ASN SEQRES 75 B 1128 PHE HIS MET PHE ASP VAL GLY GLY GLN ARG ASP GLU ARG SEQRES 76 B 1128 ARG LYS TRP ILE GLN CYS PHE ASN ASP VAL THR ALA ILE SEQRES 77 B 1128 ILE PHE VAL VAL ASP SER SER ASP TYR ASN ARG LEU GLN SEQRES 78 B 1128 GLU ALA LEU ASN ASP PHE LYS SER ILE TRP ASN ASN ARG SEQRES 79 B 1128 TRP LEU ARG THR ILE SER VAL ILE LEU PHE LEU ASN LYS SEQRES 80 B 1128 GLN ASP LEU LEU ALA GLU LYS VAL LEU ALA GLY LYS SER SEQRES 81 B 1128 LYS ILE GLU ASP TYR PHE PRO GLU PHE ALA ARG TYR THR SEQRES 82 B 1128 THR PRO GLU ASP ALA THR PRO GLU PRO GLY GLU ASP PRO SEQRES 83 B 1128 ARG VAL THR ARG ALA LYS TYR PHE ILE ARG ASP GLU PHE SEQRES 84 B 1128 LEU ARG ILE SER THR ALA SER GLY ASP GLY ARG HIS TYR SEQRES 85 B 1128 CYS TYR PRO HIS PHE THR CYS ALA VAL ASP THR GLN ASN SEQRES 86 B 1128 VAL LYS PHE VAL PHE ASP ALA VAL THR ASP ILE ILE ILE SEQRES 87 B 1128 LYS GLU ASN LEU LYS ASP CYS GLY LEU PHE SEQRES 1 C 340 MET SER GLU LEU ASP GLN LEU ARG GLN GLU ALA GLU GLN SEQRES 2 C 340 LEU LYS ASN GLN ILE ARG ASP ALA ARG LYS ALA CYS ALA SEQRES 3 C 340 ASP ALA THR LEU SER GLN ILE THR ASN ASN ILE ASP PRO SEQRES 4 C 340 VAL GLY ARG ILE GLN MET ARG THR ARG ARG THR LEU ARG SEQRES 5 C 340 GLY HIS LEU ALA LYS ILE TYR ALA MET HIS TRP GLY THR SEQRES 6 C 340 ASP SER ARG LEU LEU VAL SER ALA SER GLN ASP GLY LYS SEQRES 7 C 340 LEU ILE ILE TRP ASP SER TYR THR THR ASN LYS VAL HIS SEQRES 8 C 340 ALA ILE PRO LEU ARG SER SER TRP VAL MET THR CYS ALA SEQRES 9 C 340 TYR ALA PRO SER GLY ASN TYR VAL ALA CYS GLY GLY LEU SEQRES 10 C 340 ASP ASN ILE CYS SER ILE TYR ASN LEU LYS THR ARG GLU SEQRES 11 C 340 GLY ASN VAL ARG VAL SER ARG GLU LEU ALA GLY HIS THR SEQRES 12 C 340 GLY TYR LEU SER CYS CYS ARG PHE LEU ASP ASP ASN GLN SEQRES 13 C 340 ILE VAL THR SER SER GLY ASP THR THR CYS ALA LEU TRP SEQRES 14 C 340 ASP ILE GLU THR GLY GLN GLN THR THR THR PHE THR GLY SEQRES 15 C 340 HIS THR GLY ASP VAL MET SER LEU SER LEU ALA PRO ASP SEQRES 16 C 340 THR ARG LEU PHE VAL SER GLY ALA CYS ASP ALA SER ALA SEQRES 17 C 340 LYS LEU TRP ASP VAL ARG GLU GLY MET CYS ARG GLN THR SEQRES 18 C 340 PHE THR GLY HIS GLU SER ASP ILE ASN ALA ILE CYS PHE SEQRES 19 C 340 PHE PRO ASN GLY ASN ALA PHE ALA THR GLY SER ASP ASP SEQRES 20 C 340 ALA THR CYS ARG LEU PHE ASP LEU ARG ALA ASP GLN GLU SEQRES 21 C 340 LEU MET THR TYR SER HIS ASP ASN ILE ILE CYS GLY ILE SEQRES 22 C 340 THR SER VAL SER PHE SER LYS SER GLY ARG LEU LEU LEU SEQRES 23 C 340 ALA GLY TYR ASP ASP PHE ASN CYS ASN VAL TRP ASP ALA SEQRES 24 C 340 LEU LYS ALA ASP ARG ALA GLY VAL LEU ALA GLY HIS ASP SEQRES 25 C 340 ASN ARG VAL SER CYS LEU GLY VAL THR ASP ASP GLY MET SEQRES 26 C 340 ALA VAL ALA THR GLY SER TRP ASP SER PHE LEU LYS ILE SEQRES 27 C 340 TRP ASN SEQRES 1 D 71 MET ALA SER ASN ASN THR ALA SER ILE ALA GLN ALA ARG SEQRES 2 D 71 LYS LEU VAL GLU GLN LEU LYS MET GLU ALA ASN ILE ASP SEQRES 3 D 71 ARG ILE LYS VAL SER LYS ALA ALA ALA ASP LEU MET ALA SEQRES 4 D 71 TYR CYS GLU ALA HIS ALA LYS GLU ASP PRO LEU LEU THR SEQRES 5 D 71 PRO VAL PRO ALA SER GLU ASN PRO PHE ARG GLU LYS LYS SEQRES 6 D 71 PHE PHE CYS ALA ILE LEU SEQRES 1 E 160 MET LYS TYR LEU LEU PRO THR ALA ALA ALA GLY LEU LEU SEQRES 2 E 160 LEU LEU ALA ALA GLN PRO ALA MET ALA GLN VAL GLN LEU SEQRES 3 E 160 GLN GLU SER GLY GLY GLY LEU VAL GLN PRO GLY GLY SER SEQRES 4 E 160 LEU ARG LEU SER CYS ALA ALA SER GLY PHE THR PHE SER SEQRES 5 E 160 ASN TYR LYS MET ASN TRP VAL ARG GLN ALA PRO GLY LYS SEQRES 6 E 160 GLY LEU GLU TRP VAL SER ASP ILE SER GLN SER GLY ALA SEQRES 7 E 160 SER ILE SER TYR THR GLY SER VAL LYS GLY ARG PHE THR SEQRES 8 E 160 ILE SER ARG ASP ASN ALA LYS ASN THR LEU TYR LEU GLN SEQRES 9 E 160 MET ASN SER LEU LYS PRO GLU ASP THR ALA VAL TYR TYR SEQRES 10 E 160 CYS ALA ARG CYS PRO ALA PRO PHE THR ARG ASP CYS PHE SEQRES 11 E 160 ASP VAL THR SER THR THR TYR ALA TYR ARG GLY GLN GLY SEQRES 12 E 160 THR GLN VAL THR VAL SER SER HIS HIS HIS HIS HIS HIS SEQRES 13 E 160 GLU PRO GLU ALA SEQRES 1 A 848 MET LYS THR ILE ILE ALA LEU SER TYR ILE PHE CYS LEU SEQRES 2 A 848 VAL PHE ALA TYR PRO TYR ASP VAL PRO ASP TYR ALA ALA SEQRES 3 A 848 ALA ALA GLU PRO ALA GLU ASN SER ASP PHE TYR LEU PRO SEQRES 4 A 848 GLY ASP TYR LEU LEU GLY GLY LEU PHE SER LEU HIS ALA SEQRES 5 A 848 ASN MET LYS GLY ILE VAL HIS LEU ASN PHE LEU GLN VAL SEQRES 6 A 848 PRO MET CYS LYS GLU TYR GLU VAL LYS VAL ILE GLY TYR SEQRES 7 A 848 ASN LEU MET GLN ALA MET ARG PHE ALA VAL GLU GLU ILE SEQRES 8 A 848 ASN ASN ASP SER SER LEU LEU PRO GLY VAL LEU LEU GLY SEQRES 9 A 848 TYR GLU ILE VAL ASP VAL CYS TYR ILE SER ASN ASN VAL SEQRES 10 A 848 GLN PRO VAL LEU TYR PHE LEU ALA HIS GLU ASP ASN LEU SEQRES 11 A 848 LEU PRO ILE GLN GLU ASP TYR SER ASN TYR ILE SER ARG SEQRES 12 A 848 VAL VAL ALA VAL ILE GLY PRO ASP ASN SER GLU SER VAL SEQRES 13 A 848 MET THR VAL ALA ASN PHE LEU SER LEU PHE LEU LEU PRO SEQRES 14 A 848 GLN ILE THR TYR SER ALA ILE SER ASP GLU LEU ARG ASP SEQRES 15 A 848 LYS VAL ARG PHE PRO ALA LEU LEU ARG THR THR PRO SER SEQRES 16 A 848 ALA ASP HIS HIS ILE GLU ALA MET VAL GLN LEU MET LEU SEQRES 17 A 848 HIS PHE ARG TRP ASN TRP ILE ILE VAL LEU VAL SER SER SEQRES 18 A 848 ASP THR TYR GLY ARG ASP ASN GLY GLN LEU LEU GLY GLU SEQRES 19 A 848 ARG VAL ALA ARG ARG ASP ILE CYS ILE ALA PHE GLN GLU SEQRES 20 A 848 THR LEU PRO THR LEU GLN PRO ASN GLN ASN MET THR SER SEQRES 21 A 848 GLU GLU ARG GLN ARG LEU VAL THR ILE VAL ASP LYS LEU SEQRES 22 A 848 GLN GLN SER THR ALA ARG VAL VAL VAL VAL PHE SER PRO SEQRES 23 A 848 ASP LEU THR LEU TYR HIS PHE PHE ASN GLU VAL LEU ARG SEQRES 24 A 848 GLN ASN PHE THR GLY ALA VAL TRP ILE ALA SER GLU SER SEQRES 25 A 848 TRP ALA ILE ASP PRO VAL LEU HIS ASN LEU THR GLU LEU SEQRES 26 A 848 ARG HIS LEU GLY THR PHE LEU GLY ILE THR ILE GLN SER SEQRES 27 A 848 VAL PRO ILE PRO GLY PHE SER GLU PHE ARG GLU TRP GLY SEQRES 28 A 848 PRO GLN ALA GLY PRO PRO PRO LEU SER ARG THR SER GLN SEQRES 29 A 848 SER TYR THR CYS ASN GLN GLU CYS ASP ASN CYS LEU ASN SEQRES 30 A 848 ALA THR LEU SER PHE ASN THR ILE LEU ARG LEU SER GLY SEQRES 31 A 848 GLU ARG VAL VAL TYR SER VAL TYR SER ALA VAL TYR ALA SEQRES 32 A 848 VAL ALA HIS ALA LEU HIS SER LEU LEU GLY CYS ASP LYS SEQRES 33 A 848 SER THR CYS THR LYS ARG VAL VAL TYR PRO TRP GLN LEU SEQRES 34 A 848 LEU GLU GLU ILE TRP LYS VAL ASN PHE THR LEU LEU ASP SEQRES 35 A 848 HIS GLN ILE PHE PHE ASP PRO GLN GLY ASP VAL ALA LEU SEQRES 36 A 848 HIS LEU GLU ILE VAL GLN TRP GLN TRP ASP ARG SER GLN SEQRES 37 A 848 ASN PRO PHE GLN SER VAL ALA SER TYR TYR PRO LEU GLN SEQRES 38 A 848 ARG GLN LEU LYS ASN ILE GLN ASP ILE SER TRP HIS THR SEQRES 39 A 848 ILE ASN ASN THR ILE PRO MET SER MET CYS SER LYS ARG SEQRES 40 A 848 CYS GLN SER GLY GLN LYS LYS LYS PRO VAL GLY ILE HIS SEQRES 41 A 848 VAL CYS CYS PHE GLU CYS ILE ASP CYS LEU PRO GLY THR SEQRES 42 A 848 PHE LEU ASN HIS THR GLU ASP GLU TYR GLU CYS GLN ALA SEQRES 43 A 848 CYS PRO ASN ASN GLU TRP SER TYR GLN SER GLU THR SER SEQRES 44 A 848 CYS PHE LYS ARG GLN LEU VAL PHE LEU GLU TRP HIS GLU SEQRES 45 A 848 ALA PRO THR ILE ALA VAL ALA LEU LEU ALA ALA LEU GLY SEQRES 46 A 848 PHE LEU SER THR LEU ALA ILE LEU VAL ILE PHE TRP ARG SEQRES 47 A 848 HIS PHE GLN THR PRO ILE VAL ARG SER ALA GLY GLY PRO SEQRES 48 A 848 MET CYS PHE LEU MET LEU THR LEU LEU LEU VAL ALA TYR SEQRES 49 A 848 MET VAL VAL PRO VAL TYR VAL GLY PRO PRO LYS VAL SER SEQRES 50 A 848 THR CYS LEU CYS ARG GLN ALA LEU PHE PRO LEU CYS PHE SEQRES 51 A 848 THR ILE CYS ILE SER CYS ILE ALA VAL ARG SER PHE GLN SEQRES 52 A 848 ILE VAL CYS ALA PHE LYS MET ALA SER ARG PHE PRO ARG SEQRES 53 A 848 ALA TYR SER TYR TRP VAL ARG TYR GLN GLY PRO TYR VAL SEQRES 54 A 848 SER MET ALA PHE ILE THR VAL LEU LYS MET VAL ILE VAL SEQRES 55 A 848 VAL ILE GLY MET LEU ALA THR GLY LEU SER PRO THR THR SEQRES 56 A 848 ARG THR ASP PRO ASP ASP PRO LYS ILE THR ILE VAL SER SEQRES 57 A 848 CYS ASN PRO ASN TYR ARG ASN SER LEU LEU PHE ASN THR SEQRES 58 A 848 SER LEU ASP LEU LEU LEU SER VAL VAL GLY PHE SER PHE SEQRES 59 A 848 ALA TYR MET GLY LYS GLU LEU PRO THR ASN TYR ASN GLU SEQRES 60 A 848 ALA LYS PHE ILE THR LEU SER MET THR PHE TYR PHE THR SEQRES 61 A 848 SER SER VAL SER LEU CYS THR PHE MET SER ALA TYR SER SEQRES 62 A 848 GLY VAL LEU VAL THR ILE VAL ASP LEU LEU VAL THR VAL SEQRES 63 A 848 LEU ASN LEU LEU ALA ILE SER LEU GLY TYR PHE GLY PRO SEQRES 64 A 848 LYS CYS TYR MET ILE LEU PHE TYR PRO GLU ARG ASN THR SEQRES 65 A 848 PRO ALA TYR PHE ASN SER MET ILE GLN GLY TYR THR MET SEQRES 66 A 848 ARG ARG ASP HELIX 1 AA1 GLU B 7 ARG B 35 1 29 HELIX 2 AA2 GLY B 49 HIS B 61 1 13 HELIX 3 AA3 LYS B 99 ASP B 106 5 8 HELIX 4 AA4 ARG B 121 ASN B 134 1 14 HELIX 5 AA5 LYS B 149 GLY B 160 1 12 HELIX 6 AA6 LYS B 163 PHE B 168 1 6 HELIX 7 AA7 PRO B 169 ALA B 172 5 4 HELIX 8 AA8 ASP B 187 GLY B 209 1 23 HELIX 9 AA9 THR B 225 CYS B 247 1 23 HELIX 10 AB1 GLU C 3 ALA C 26 1 24 HELIX 11 AB2 THR C 29 THR C 34 1 6 HELIX 12 AB3 ASN C 35 ILE C 37 5 3 HELIX 13 AB4 SER D 8 ALA D 23 1 16 HELIX 14 AB5 LYS D 29 HIS D 44 1 16 HELIX 15 AB6 ALA D 45 ASP D 48 5 4 HELIX 16 AB7 PRO D 55 ASN D 59 5 5 HELIX 17 AB8 THR E 28 TYR E 32 5 5 HELIX 18 AB9 LYS E 87 THR E 91 5 5 HELIX 19 AC1 GLU A 563 HIS A 590 1 28 HELIX 20 AC2 THR A 593 GLY A 600 1 8 HELIX 21 AC3 GLY A 601 VAL A 617 1 17 HELIX 22 AC4 VAL A 617 VAL A 622 1 6 HELIX 23 AC5 LYS A 626 LEU A 636 1 11 HELIX 24 AC6 LEU A 636 LYS A 660 1 25 HELIX 25 AC7 PHE A 665 TYR A 675 1 11 HELIX 26 AC8 GLN A 676 ALA A 699 1 24 HELIX 27 AC9 ARG A 725 TYR A 747 1 23 HELIX 28 AD1 MET A 748 LYS A 750 5 3 HELIX 29 AD2 GLU A 758 ALA A 782 1 25 HELIX 30 AD3 LEU A 787 TYR A 818 1 32 HELIX 31 AD4 PRO A 819 ASN A 822 5 4 HELIX 32 AD5 THR A 823 MET A 836 1 14 SHEET 1 AA1 6 THR B 76 GLN B 79 0 SHEET 2 AA1 6 ASN B 84 ASP B 89 -1 O MET B 87 N THR B 76 SHEET 3 AA1 6 THR B 37 LEU B 43 1 N LEU B 40 O HIS B 86 SHEET 4 AA1 6 ALA B 109 ASP B 115 1 O ILE B 111 N LEU B 41 SHEET 5 AA1 6 SER B 142 ASN B 148 1 O PHE B 146 N PHE B 112 SHEET 6 AA1 6 CYS B 215 PHE B 219 1 O TYR B 216 N VAL B 143 SHEET 1 AA2 4 ARG C 46 ARG C 52 0 SHEET 2 AA2 4 PHE C 335 ASN C 340 -1 O LEU C 336 N LEU C 51 SHEET 3 AA2 4 VAL C 327 SER C 331 -1 N VAL C 327 O TRP C 339 SHEET 4 AA2 4 VAL C 315 VAL C 320 -1 N GLY C 319 O ALA C 328 SHEET 1 AA3 4 ILE C 58 TRP C 63 0 SHEET 2 AA3 4 LEU C 69 SER C 74 -1 O ALA C 73 N TYR C 59 SHEET 3 AA3 4 LYS C 78 ASP C 83 -1 O TRP C 82 N LEU C 70 SHEET 4 AA3 4 ASN C 88 PRO C 94 -1 O VAL C 90 N ILE C 81 SHEET 1 AA4 4 VAL C 100 TYR C 105 0 SHEET 2 AA4 4 TYR C 111 GLY C 116 -1 O ALA C 113 N ALA C 104 SHEET 3 AA4 4 ILE C 120 ASN C 125 -1 O TYR C 124 N VAL C 112 SHEET 4 AA4 4 ARG C 134 ALA C 140 -1 O SER C 136 N ILE C 123 SHEET 1 AA5 4 LEU C 146 ASP C 153 0 SHEET 2 AA5 4 GLN C 156 SER C 161 -1 O VAL C 158 N ARG C 150 SHEET 3 AA5 4 THR C 165 ASP C 170 -1 O TRP C 169 N ILE C 157 SHEET 4 AA5 4 GLN C 175 THR C 181 -1 O GLN C 175 N ASP C 170 SHEET 1 AA6 4 SER C 191 LEU C 192 0 SHEET 2 AA6 4 LEU C 198 GLY C 202 -1 O VAL C 200 N SER C 191 SHEET 3 AA6 4 ALA C 208 ASP C 212 -1 O TRP C 211 N PHE C 199 SHEET 4 AA6 4 CYS C 218 PHE C 222 -1 O PHE C 222 N ALA C 208 SHEET 1 AA7 4 ILE C 229 PHE C 234 0 SHEET 2 AA7 4 ALA C 240 SER C 245 -1 O ALA C 242 N CYS C 233 SHEET 3 AA7 4 CYS C 250 ASP C 254 -1 O PHE C 253 N PHE C 241 SHEET 4 AA7 4 GLN C 259 TYR C 264 -1 O LEU C 261 N LEU C 252 SHEET 1 AA8 4 ILE C 273 PHE C 278 0 SHEET 2 AA8 4 LEU C 284 TYR C 289 -1 O GLY C 288 N SER C 275 SHEET 3 AA8 4 CYS C 294 ASP C 298 -1 O ASN C 295 N ALA C 287 SHEET 4 AA8 4 ARG C 304 LEU C 308 -1 O LEU C 308 N CYS C 294 SHEET 1 AA9 4 GLN E 3 SER E 7 0 SHEET 2 AA9 4 SER E 17 SER E 25 -1 O SER E 25 N GLN E 3 SHEET 3 AA9 4 THR E 78 ASN E 84 -1 O MET E 83 N LEU E 18 SHEET 4 AA9 4 PHE E 68 ASP E 73 -1 N SER E 71 O TYR E 80 SHEET 1 AB1 6 LEU E 11 VAL E 12 0 SHEET 2 AB1 6 THR E 122 VAL E 126 1 O THR E 125 N VAL E 12 SHEET 3 AB1 6 ALA E 92 ARG E 98 -1 N TYR E 94 O THR E 122 SHEET 4 AB1 6 MET E 34 GLN E 39 -1 N VAL E 37 O TYR E 95 SHEET 5 AB1 6 GLU E 46 ILE E 51 -1 O GLU E 46 N ARG E 38 SHEET 6 AB1 6 ILE E 58 TYR E 60 -1 O SER E 59 N ASP E 50 SSBOND 1 CYS E 22 CYS E 96 1555 1555 2.03 SSBOND 2 CYS E 99 CYS E 107 1555 1555 2.03 CRYST1 1.000 1.000 1.000 90.00 90.00 90.00 P 1 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 1.000000 0.000000 0.000000 0.00000 SCALE2 0.000000 1.000000 0.000000 0.00000 SCALE3 0.000000 0.000000 1.000000 0.00000