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The relative orientation between the variable domains (VH and VL) influences the topology of the antigen binding site. It is important to consider when building high-resolution models of antibodies.
The VH/VL orientation can be represented by six measures:
| HL | the torsion angle between H1 and L1 |
| HC1 | the bend angle between H1 and C |
| HC2 | the bend angle between H2 and C |
| LC1 | the bend angle between L1 and C |
| LC2 | the bend angle between L2 and C |
| dc | the length of C |
ABangle is a tool that calculates these angles from a PDB structure. Use the search forms below to use it to select antibodies based on their VH/VL orientation.
> Search the database by angle
Click on parts of each graph to select the range of angles to search for.
> Get the orientation of a specific PDB entry
Input a PDB code. To select a specific Fv, use its chain identifiers - for example, 12e8_HL.
