Structure of S67-27 in complex with Kdo(2.8)-7-O-Methyl-Kdo
PDB | 3ikc |
Species | MUS MUSCULUS |
Method | X-RAY DIFFRACTION |
Resolution | 2.6Å |
Number of Fvs | 2 |
In complex | True |
Light chain type | Kappa |
Has constant region | True |
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Display options: |
This PDB has 2 Fv(s).
Fv Details | |
---|---|
Heavy chain | B |
Light chain | A |
Heavy subgroup | IGHV7 |
Light subgroup | IGKV8 |
Species | MUS MUSCULUS |
In complex? | True |
scFv? | False |
Has constant domain? | True |
Numbered Sequences (chothia) |
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Heavy chain
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 52A | 52B | 52C | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 82A | 82B | 82C | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 100A | 100B | 100C | 100D | 100E | 100F | 100G | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | 109 | 110 | 111 | 112 |
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E | V | M | L | V | E | S | G | G | G | L | V | Q | P | G | N | S | L | R | L | S | C | A | T | S | G | F | T | F | T | D | Y | Y | M | S | W | V | R | Q | P | P | G | K | A | L | E | W | L | G | F | I | R | N | K | A | K | G | Y | T | T | E | Y | S | A | S | V | K | G | R | F | T | I | S | R | D | N | S | Q | S | I | L | Y | L | Q | M | N | T | L | R | A | E | D | S | A | T | Y | Y | C | A | R | D | I | S | P | S | Y | G | V | Y | Y | E | G | F | A | Y | W | G | Q | G | T | L | V | T | V | S |
Light chain
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 30A | 30B | 30C | 30D | 30E | 30F | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 | 106 | 107 |
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D | I | V | M | T | Q | S | P | S | S | L | A | V | S | A | G | E | K | V | T | M | S | C | K | S | S | Q | S | L | L | N | S | R | T | R | K | N | Y | L | A | W | Y | Q | Q | K | P | G | Q | S | P | K | L | L | I | Y | W | A | S | T | R | E | S | G | V | P | D | R | F | T | G | S | G | S | G | T | D | F | T | L | T | I | S | S | V | Q | A | E | D | L | A | V | Y | Y | C | K | Q | S | N | N | L | R | T | F | G | G | G | T | K | L | E | I | K |
Antigen Details | |
---|---|
Antigen chains | B |
Antigen type | Hapten |
Antigen name | (1E)-PROP-1-EN-1-YL 3-DEOXY-7-O-METHYL-ALPHA-D-MANNO-OCT-2-ULOPYRANOSIDONIC ACID |
Antigen species | MUS MUSCULUS |
Antigen sequence |
CDR Sequences (chothia definition) | |
---|---|
CDRH1 | GFTFTDY |
CDRH2 | RNKAKGYT |
CDRH3 | DISPSYGVYYEGFAY |
CDRL1 | KSSQSLLNSRTRKNYLA |
CDRL2 | WASTRES |
CDRL3 | KQSNNLRT |
Orientation Angles (from ABangle) | |
---|---|
HL | -57.66° |
HC1 | 71.16° |
HC2 | 120.73° |
LC1 | 122.09° |
LC2 | 80.37° |
dc | 16.0Å |
Fv Details | |
---|---|
Heavy chain | D |
Light chain | C |
Heavy subgroup | IGHV7 |
Light subgroup | IGKV8 |
Species | MUS MUSCULUS |
In complex? | True |
scFv? | False |
Has constant domain? | True |
Numbered Sequences (chothia) |
---|
Heavy chain
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 52A | 52B | 52C | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 82A | 82B | 82C | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 100A | 100B | 100C | 100D | 100E | 100F | 100G | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | 109 | 110 | 111 | 112 |
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E | V | M | L | V | E | S | G | G | G | L | V | Q | P | G | N | S | L | R | L | S | C | A | T | S | G | F | T | F | T | D | Y | Y | M | S | W | V | R | Q | P | P | G | K | A | L | E | W | L | G | F | I | R | N | K | A | K | G | Y | T | T | E | Y | S | A | S | V | K | G | R | F | T | I | S | R | D | N | S | Q | S | I | L | Y | L | Q | M | N | T | L | R | A | E | D | S | A | T | Y | Y | C | A | R | D | I | S | P | S | Y | G | V | Y | Y | E | G | F | A | Y | W | G | Q | G | T | L | V | T | V | S |
Light chain
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 30A | 30B | 30C | 30D | 30E | 30F | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 | 106 | 107 |
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D | I | V | M | T | Q | S | P | S | S | L | A | V | S | A | G | E | K | V | T | M | S | C | K | S | S | Q | S | L | L | N | S | R | T | R | K | N | Y | L | A | W | Y | Q | Q | K | P | G | Q | S | P | K | L | L | I | Y | W | A | S | T | R | E | S | G | V | P | D | R | F | T | G | S | G | S | G | T | D | F | T | L | T | I | S | S | V | Q | A | E | D | L | A | V | Y | Y | C | K | Q | S | N | N | L | R | T | F | G | G | G | T | K | L | E | I | K |
Antigen Details | |
---|---|
Antigen chains | C |
Antigen type | carbohydrate |
Antigen name | 3-DEOXY-D-MANNO-OCT-2-ULOSONIC ACID |
Antigen species | MUS MUSCULUS |
Antigen sequence |
CDR Sequences (chothia definition) | |
---|---|
CDRH1 | GFTFTDY |
CDRH2 | RNKAKGYT |
CDRH3 | DISPSYGVYYEGFAY |
CDRL1 | KSSQSLLNSRTRKNYLA |
CDRL2 | WASTRES |
CDRL3 | KQSNNLRT |
Orientation Angles (from ABangle) | |
---|---|
HL | -59.41° |
HC1 | 70.67° |
HC2 | 119.3° |
LC1 | 121.03° |
LC2 | 81.75° |
dc | 16.03Å |
Schneider, C., Raybould, M.I.J., Deane, C.M. (2022) SAbDab in the Age of Biotherapeutics: Updates including SAbDab-Nano, the Nanobody Structure Tracker. Nucleic Acids Res. 50(D1):D1368-D1372 [link]
SAbDab paper: Dunbar, J., Krawczyk, K. et al (2014). Nucleic Acids Res. 42. D1140-D1146 [link]
Thera-SAbDab paper: Raybould, M.I.J., Marks, C. et al (2019). Nucleic Acids Res. gkz827 [link]