Structure Viewer: 4rdq

> Structure details

Calcium-activated Chloride Channel Bestrophin-1, From Chicken, in complex with Fab antibody Fragments, Chloride and Calcium

PDB	4rdq
Species	MUS MUSCULUS
Method	X-RAY DIFFRACTION
Resolution	2.85Å
Number of Fvs	5
In complex	True
Light chain type	Kappa
Has constant region	True

> Fv information

This PDB has 5 Fv(s).

Fv Details
Heavy chain	G
Light chain	F
Heavy subgroup	IGHV1
Light subgroup	IGKV12
Species	MUS MUSCULUS
In complex?	True
scFv?	False
Has constant domain?	True

Numbered Sequences (chothia)

Heavy chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	101	102	103	104	105	106	107	108	109	110	111	112	113
Q	V	Q	L	Q	Q	S	G	P	E	L	V	R	P	G	A	S	V	K	M	S	C	K	A	S	G	Y	T	F	T	N	Y	W	M	H	W	V	K	Q	R	P	G	Q	A	L	E	W	I	G	M	I	D	P	S	K	S	E	T	T	L	N	Q	K	F	R	G	K	A	T	L	N	V	D	K	S	S	N	T	A	Y	M	Q	L	S	S	L	T	S	E	D	S	A	V	Y	Y	C	A	R	E	V	Y	Y	F	D	Y	W	G	Q	G	T	T	L	T	V	S	S

Light chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
D	I	Q	M	T	Q	S	P	A	S	L	S	A	S	V	G	E	T	V	T	I	T	C	R	A	S	E	N	I	Y	S	Y	L	T	W	Y	Q	Q	K	Q	G	K	S	P	Q	L	L	V	Y	N	A	K	T	L	T	E	G	V	P	S	R	F	S	G	S	G	S	G	T	Q	F	S	L	K	I	N	S	L	Q	P	E	D	F	G	G	Y	F	C	Q	H	H	Y	G	T	P	P	T	F	G	G	G	T	K	L	E	V	K

Antigen Details
Antigen chains	E
Antigen type	protein
Antigen name	bestrophin-1
Antigen species	GALLUS GALLUS
Antigen sequence	TVTYTNRVADARLGTFSQLLLQWKGSIYKLLYSEFLIFISLYFAISLVYRLILSESQRLMFEKLALYCNSYAELIPVSFVLGFYVSLVVSRWWAQYESIPWPDRIMNLVSCNVDGEDEYGRLLRRTLMRYSNLCSVLILRSVSTAVYKRFPSMEHVVRAGLMTPEEHKKFESLNSPHNKFWIPCVWFSNLAVKARNEGRIRDSVLLQGILNELNTLRSQCGRLYGYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLDPEKAYPGHELDLFVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNLQVSLMAVDEMHQDLPILEKDLYWNEPDPQPPYTAATAEYKRPSFLGSTFDISMQKEEMEFQPLEQIKENEEANHSTPLLGHLGRLLGVQSEGEEF

CDR Sequences (chothia definition)
CDRH1	GYTFTNY
CDRH2	DPSKSE
CDRH3	EVYYFDY
CDRL1	RASENIYSYLT
CDRL2	NAKTLTE
CDRL3	QHHYGTPPT

Orientation Angles (from ABangle)
HL	-59.26°
HC1	71.46°
HC2	112.2°
LC1	119.5°
LC2	79.94°
dc	16.43Å

Fv Details
Heavy chain	M
Light chain	L
Heavy subgroup	IGHV1
Light subgroup	IGKV12
Species	MUS MUSCULUS
In complex?	True
scFv?	False
Has constant domain?	True

Numbered Sequences (chothia)

Heavy chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	101	102	103	104	105	106	107	108	109	110	111	112	113
Q	V	Q	L	Q	Q	S	G	P	E	L	V	R	P	G	A	S	V	K	M	S	C	K	A	S	G	Y	T	F	T	N	Y	W	M	H	W	V	K	Q	R	P	G	Q	A	L	E	W	I	G	M	I	D	P	S	K	S	E	T	T	L	N	Q	K	F	R	G	K	A	T	L	N	V	D	K	S	S	N	T	A	Y	M	Q	L	S	S	L	T	S	E	D	S	A	V	Y	Y	C	A	R	E	V	Y	Y	F	D	Y	W	G	Q	G	T	T	L	T	V	S	S

Light chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
D	I	Q	M	T	Q	S	P	A	S	L	S	A	S	V	G	E	T	V	T	I	T	C	R	A	S	E	N	I	Y	S	Y	L	T	W	Y	Q	Q	K	Q	G	K	S	P	Q	L	L	V	Y	N	A	K	T	L	T	E	G	V	P	S	R	F	S	G	S	G	S	G	T	Q	F	S	L	K	I	N	S	L	Q	P	E	D	F	G	G	Y	F	C	Q	H	H	Y	G	T	P	P	T	F	G	G	G	T	K	L	E	V	K

Antigen Details
Antigen chains	C
Antigen type	protein
Antigen name	bestrophin-1
Antigen species	GALLUS GALLUS
Antigen sequence	TVTYTNRVADARLGTFSQLLLQWKGSIYKLLYSEFLIFISLYFAISLVYRLILSESQRLMFEKLALYCNSYAELIPVSFVLGFYVSLVVSRWWAQYESIPWPDRIMNLVSCNVDGEDEYGRLLRRTLMRYSNLCSVLILRSVSTAVYKRFPSMEHVVRAGLMTPEEHKKFESLNSPHNKFWIPCVWFSNLAVKARNEGRIRDSVLLQGILNELNTLRSQCGRLYGYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLDPEKAYPGHELDLFVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNLQVSLMAVDEMHQDLPILEKDLYWNEPDPQPPYTAATAEYKRPSFLGSTFDISMQKEEMEFQPLEQIKENEEANHSTPLLGHLGRLLGVQSEGEEF

CDR Sequences (chothia definition)
CDRH1	GYTFTNY
CDRH2	DPSKSE
CDRH3	EVYYFDY
CDRL1	RASENIYSYLT
CDRL2	NAKTLTE
CDRL3	QHHYGTPPT

Orientation Angles (from ABangle)
HL	-59.08°
HC1	71.38°
HC2	112.2°
LC1	119.54°
LC2	80.08°
dc	16.43Å

Fv Details
Heavy chain	O
Light chain	N
Heavy subgroup	IGHV1
Light subgroup	IGKV12
Species	MUS MUSCULUS
In complex?	True
scFv?	False
Has constant domain?	True

Numbered Sequences (chothia)

Heavy chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	101	102	103	104	105	106	107	108	109	110	111	112	113
Q	V	Q	L	Q	Q	S	G	P	E	L	V	R	P	G	A	S	V	K	M	S	C	K	A	S	G	Y	T	F	T	N	Y	W	M	H	W	V	K	Q	R	P	G	Q	A	L	E	W	I	G	M	I	D	P	S	K	S	E	T	T	L	N	Q	K	F	R	G	K	A	T	L	N	V	D	K	S	S	N	T	A	Y	M	Q	L	S	S	L	T	S	E	D	S	A	V	Y	Y	C	A	R	E	V	Y	Y	F	D	Y	W	G	Q	G	T	T	L	T	V	S	S

Light chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
D	I	Q	M	T	Q	S	P	A	S	L	S	A	S	V	G	E	T	V	T	I	T	C	R	A	S	E	N	I	Y	S	Y	L	T	W	Y	Q	Q	K	Q	G	K	S	P	Q	L	L	V	Y	N	A	K	T	L	T	E	G	V	P	S	R	F	S	G	S	G	S	G	T	Q	F	S	L	K	I	N	S	L	Q	P	E	D	F	G	G	Y	F	C	Q	H	H	Y	G	T	P	P	T	F	G	G	G	T	K	L	E	V	K

Antigen Details
Antigen chains	D
Antigen type	protein
Antigen name	bestrophin-1
Antigen species	GALLUS GALLUS
Antigen sequence	TVTYTNRVADARLGTFSQLLLQWKGSIYKLLYSEFLIFISLYFAISLVYRLILSESQRLMFEKLALYCNSYAELIPVSFVLGFYVSLVVSRWWAQYESIPWPDRIMNLVSCNVDGEDEYGRLLRRTLMRYSNLCSVLILRSVSTAVYKRFPSMEHVVRAGLMTPEEHKKFESLNSPHNKFWIPCVWFSNLAVKARNEGRIRDSVLLQGILNELNTLRSQCGRLYGYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLDPEKAYPGHELDLFVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNLQVSLMAVDEMHQDLPILEKDLYWNEPDPQPPYTAATAEYKRPSFLGSTFDISMQKEEMEFQPLEQIKENEEANHSTPLLGHLGRLLGVQSEGEEF

CDR Sequences (chothia definition)
CDRH1	GYTFTNY
CDRH2	DPSKSE
CDRH3	EVYYFDY
CDRL1	RASENIYSYLT
CDRL2	NAKTLTE
CDRL3	QHHYGTPPT

Orientation Angles (from ABangle)
HL	-59.18°
HC1	71.53°
HC2	112.07°
LC1	119.48°
LC2	79.91°
dc	16.4Å

Fv Details
Heavy chain	K
Light chain	J
Heavy subgroup	IGHV1
Light subgroup	IGKV12
Species	MUS MUSCULUS
In complex?	True
scFv?	False
Has constant domain?	True

Numbered Sequences (chothia)

Heavy chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	101	102	103	104	105	106	107	108	109	110	111	112	113
Q	V	Q	L	Q	Q	S	G	P	E	L	V	R	P	G	A	S	V	K	M	S	C	K	A	S	G	Y	T	F	T	N	Y	W	M	H	W	V	K	Q	R	P	G	Q	A	L	E	W	I	G	M	I	D	P	S	K	S	E	T	T	L	N	Q	K	F	R	G	K	A	T	L	N	V	D	K	S	S	N	T	A	Y	M	Q	L	S	S	L	T	S	E	D	S	A	V	Y	Y	C	A	R	E	V	Y	Y	F	D	Y	W	G	Q	G	T	T	L	T	V	S	S

Light chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
D	I	Q	M	T	Q	S	P	A	S	L	S	A	S	V	G	E	T	V	T	I	T	C	R	A	S	E	N	I	Y	S	Y	L	T	W	Y	Q	Q	K	Q	G	K	S	P	Q	L	L	V	Y	N	A	K	T	L	T	E	G	V	P	S	R	F	S	G	S	G	S	G	T	Q	F	S	L	K	I	N	S	L	Q	P	E	D	F	G	G	Y	F	C	Q	H	H	Y	G	T	P	P	T	F	G	G	G	T	K	L	E	V	K

Antigen Details
Antigen chains	B
Antigen type	protein
Antigen name	bestrophin-1
Antigen species	GALLUS GALLUS
Antigen sequence	TVTYTNRVADARLGTFSQLLLQWKGSIYKLLYSEFLIFISLYFAISLVYRLILSESQRLMFEKLALYCNSYAELIPVSFVLGFYVSLVVSRWWAQYESIPWPDRIMNLVSCNVDGEDEYGRLLRRTLMRYSNLCSVLILRSVSTAVYKRFPSMEHVVRAGLMTPEEHKKFESLNSPHNKFWIPCVWFSNLAVKARNEGRIRDSVLLQGILNELNTLRSQCGRLYGYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLDPEKAYPGHELDLFVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNLQVSLMAVDEMHQDLPILEKDLYWNEPDPQPPYTAATAEYKRPSFLGSTFDISMQKEEMEFQPLEQIKENEEANHSTPLLGHLGRLLGVQSEGEEF

CDR Sequences (chothia definition)
CDRH1	GYTFTNY
CDRH2	DPSKSE
CDRH3	EVYYFDY
CDRL1	RASENIYSYLT
CDRL2	NAKTLTE
CDRL3	QHHYGTPPT

Orientation Angles (from ABangle)
HL	-59.16°
HC1	71.46°
HC2	112.21°
LC1	119.59°
LC2	80.05°
dc	16.42Å

Fv Details
Heavy chain	I
Light chain	H
Heavy subgroup	IGHV1
Light subgroup	IGKV12
Species	MUS MUSCULUS
In complex?	True
scFv?	False
Has constant domain?	True

Numbered Sequences (chothia)

Heavy chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	101	102	103	104	105	106	107	108	109	110	111	112	113
Q	V	Q	L	Q	Q	S	G	P	E	L	V	R	P	G	A	S	V	K	M	S	C	K	A	S	G	Y	T	F	T	N	Y	W	M	H	W	V	K	Q	R	P	G	Q	A	L	E	W	I	G	M	I	D	P	S	K	S	E	T	T	L	N	Q	K	F	R	G	K	A	T	L	N	V	D	K	S	S	N	T	A	Y	M	Q	L	S	S	L	T	S	E	D	S	A	V	Y	Y	C	A	R	E	V	Y	Y	F	D	Y	W	G	Q	G	T	T	L	T	V	S	S

Light chain

1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
D	I	Q	M	T	Q	S	P	A	S	L	S	A	S	V	G	E	T	V	T	I	T	C	R	A	S	E	N	I	Y	S	Y	L	T	W	Y	Q	Q	K	Q	G	K	S	P	Q	L	L	V	Y	N	A	K	T	L	T	E	G	V	P	S	R	F	S	G	S	G	S	G	T	Q	F	S	L	K	I	N	S	L	Q	P	E	D	F	G	G	Y	F	C	Q	H	H	Y	G	T	P	P	T	F	G	G	G	T	K	L	E	V	K

Antigen Details
Antigen chains	A
Antigen type	protein
Antigen name	bestrophin-1
Antigen species	GALLUS GALLUS
Antigen sequence	TVTYTNRVADARLGTFSQLLLQWKGSIYKLLYSEFLIFISLYFAISLVYRLILSESQRLMFEKLALYCNSYAELIPVSFVLGFYVSLVVSRWWAQYESIPWPDRIMNLVSCNVDGEDEYGRLLRRTLMRYSNLCSVLILRSVSTAVYKRFPSMEHVVRAGLMTPEEHKKFESLNSPHNKFWIPCVWFSNLAVKARNEGRIRDSVLLQGILNELNTLRSQCGRLYGYDWISIPLVYTQVVTVAVYSFFLACLIGRQFLDPEKAYPGHELDLFVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWLIDRNLQVSLMAVDEMHQDLPILEKDLYWNEPDPQPPYTAATAEYKRPSFLGSTFDISMQKEEMEFQPLEQIKENEEANHSTPLLGHLGRLLGVQSEGEEF

CDR Sequences (chothia definition)
CDRH1	GYTFTNY
CDRH2	DPSKSE
CDRH3	EVYYFDY
CDRL1	RASENIYSYLT
CDRL2	NAKTLTE
CDRL3	QHHYGTPPT

Orientation Angles (from ABangle)
HL	-59.2°
HC1	71.48°
HC2	112.17°
LC1	119.54°
LC2	80.01°
dc	16.42Å

Additional links and files for download: see help for more details.

Chothia-numbered structure	Download
IMGT-numbered structure	Download
Non-annotated structure from the PDB	Download
Summary file for this antibody	Download

Schneider, C., Raybould, M.I.J., Deane, C.M. (2022) SAbDab in the Age of Biotherapeutics: Updates including SAbDab-Nano, the Nanobody Structure Tracker. Nucleic Acids Res. 50(D1):D1368-D1372 [link]

SAbDab paper: Dunbar, J., Krawczyk, K. et al (2014). Nucleic Acids Res. 42. D1140-D1146 [link]

Thera-SAbDab paper: Raybould, M.I.J., Marks, C. et al (2019). Nucleic Acids Res. gkz827 [link]

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