Crystal Structure of Human Two Pore Domain Potassium Ion Channel Trek-2 (K2P10.1) in complex with an activatory Nanobody (Nb67)
| PDB | 8qz3 |
| Species | LAMA GLAMA |
| Method | X-RAY DIFFRACTION |
| Resolution | 2.4Å |
| Number of Fvs | 3 |
| In complex | True |
| Light chain type | NA |
| Has constant region | False |
|
|
Display options: |
This PDB has 3 Fv(s).
| Fv Details | |
|---|---|
| Heavy chain | C |
| Light chain | |
| Heavy subgroup | IGHV1 |
| Light subgroup | NA |
| Species | LAMA GLAMA |
| In complex? | True |
| scFv? | False |
| Has constant domain? | False |
| Numbered Sequences (chothia) |
|---|
Heavy chain
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 52A | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 82A | 82B | 82C | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 100A | 100B | 100C | 100D | 100E | 100F | 100G | 100H | 100I | 100J | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q | V | Q | L | V | E | S | G | G | G | L | V | Q | A | G | G | S | L | R | L | S | C | A | A | S | G | R | A | F | S | T | Y | V | M | G | W | F | R | E | A | P | G | K | E | R | D | F | V | A | T | L | S | R | G | G | A | V | T | Y | Y | A | D | S | V | K | G | R | F | T | I | S | R | D | N | A | K | N | T | V | Y | L | Q | M | D | S | L | E | P | E | D | T | A | V | Y | Y | C | A | A | R | D | R | L | G | G | A | G | T | A | T | F | W | G | D | Y | D | Y | W | G | Q | G | T | Q | V | T | V | S | S |
| Antigen Details | |
|---|---|
| Antigen chains | B |
| Antigen type | protein |
| Antigen name | potassium channel subfamily k member 10 |
| Antigen species | HOMO SAPIENS |
| Antigen sequence | MKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVSPIGQSSQQSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEAENLYFQ |
| CDR Sequences (chothia definition) | |
|---|---|
| CDRH1 | GRAFSTY |
| CDRH2 | SRGGAV |
| CDRH3 | RDRLGGAGTATFWGDYDY |
| Fv Details | |
|---|---|
| Heavy chain | D |
| Light chain | |
| Heavy subgroup | IGHV1 |
| Light subgroup | NA |
| Species | LAMA GLAMA |
| In complex? | True |
| scFv? | False |
| Has constant domain? | False |
| Numbered Sequences (chothia) |
|---|
Heavy chain
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 52A | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 82A | 82B | 82C | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 100A | 100B | 100C | 100D | 100E | 100F | 100G | 100H | 100I | 100J | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q | V | Q | L | V | E | S | G | G | G | L | V | Q | A | G | G | S | L | R | L | S | C | A | A | S | G | R | A | F | S | T | Y | V | M | G | W | F | R | E | A | P | G | K | E | R | D | F | V | A | T | L | S | R | G | G | A | V | T | Y | Y | A | D | S | V | K | G | R | F | T | I | S | R | D | N | A | K | N | T | V | Y | L | Q | M | D | S | L | E | P | E | D | T | A | V | Y | Y | C | A | A | R | D | R | L | G | G | A | G | T | A | T | F | W | G | D | Y | D | Y | W | G | Q | G | T | Q | V | T | V | S | S |
| Antigen Details | |
|---|---|
| Antigen chains | B |
| Antigen type | protein |
| Antigen name | potassium channel subfamily k member 10 |
| Antigen species | HOMO SAPIENS |
| Antigen sequence | MKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVSPIGQSSQQSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEAENLYFQ |
| CDR Sequences (chothia definition) | |
|---|---|
| CDRH1 | GRAFSTY |
| CDRH2 | SRGGAV |
| CDRH3 | RDRLGGAGTATFWGDYDY |
| Fv Details | |
|---|---|
| Heavy chain | E |
| Light chain | |
| Heavy subgroup | IGHV1 |
| Light subgroup | NA |
| Species | LAMA GLAMA |
| In complex? | True |
| scFv? | False |
| Has constant domain? | False |
| Numbered Sequences (chothia) |
|---|
Heavy chain
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 52A | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 82A | 82B | 82C | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 100A | 100B | 100C | 100D | 100E | 100F | 100G | 100H | 100I | 100J | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q | V | Q | L | V | E | S | G | G | G | L | V | Q | A | G | G | S | L | R | L | S | C | A | A | S | G | R | A | F | S | T | Y | V | M | G | W | F | R | E | A | P | G | K | E | R | D | F | V | A | T | L | S | R | G | G | A | V | T | Y | Y | A | D | S | V | K | G | R | F | T | I | S | R | D | N | A | K | N | T | V | Y | L | Q | M | D | S | L | E | P | E | D | T | A | V | Y | Y | C | A | A | R | D | R | L | G | G | A | G | T | A | T | F | W | G | D | Y | D | Y | W | G | Q | G | T | Q | V | T | V | S | S |
| Antigen Details | |
|---|---|
| Antigen chains | A |
| Antigen type | protein |
| Antigen name | potassium channel subfamily k member 10 |
| Antigen species | HOMO SAPIENS |
| Antigen sequence | MKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVSPIGQSSQQSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEAENLYFQ |
| CDR Sequences (chothia definition) | |
|---|---|
| CDRH1 | GRAFSTY |
| CDRH2 | SRGGAV |
| CDRH3 | RDRLGGAGTATFWGDYDY |
Schneider, C., Raybould, M.I.J., Deane, C.M. (2022) SAbDab in the Age of Biotherapeutics: Updates including SAbDab-Nano, the Nanobody Structure Tracker. Nucleic Acids Res. 50(D1):D1368-D1372 [link]
SAbDab paper: Dunbar, J., Krawczyk, K. et al (2014). Nucleic Acids Res. 42. D1140-D1146 [link]
Thera-SAbDab paper: Raybould, M.I.J., Marks, C. et al (2019). Nucleic Acids Res. gkz827 [link]
