Cryo-Em Structure of the Pea-Bound Mtaar1-Gs complex
PDB | 8wc6 |
Species | SYNTHETIC CONSTRUCT |
Method | ELECTRON MICROSCOPY |
Resolution | 3.2Å |
Number of Fvs | 2 |
In complex | True |
Light chain type | NA,unknown |
Has constant region | False |
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Display options: |
This PDB has 2 Fv(s).
Fv Details | |
---|---|
Heavy chain | S |
Light chain | S |
Heavy subgroup | unknown |
Light subgroup | unknown |
Species | SYNTHETIC CONSTRUCT |
In complex? | True |
scFv? | True |
Has constant domain? | False |
Numbered Sequences (chothia) |
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Heavy chain
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 52A | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 82A | 82B | 82C | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 100A | 100B | 100C | 100D | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 |
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A | V | Q | L | V | E | S | G | G | G | L | V | Q | P | G | G | S | R | K | L | S | C | S | A | S | G | F | A | F | S | S | F | G | M | H | W | V | R | Q | A | P | E | K | G | L | E | W | V | A | Y | I | S | S | G | S | G | T | I | Y | Y | A | D | T | V | K | G | R | F | T | I | S | R | D | D | P | K | N | T | L | F | L | Q | M | T | S | L | R | S | E | D | T | A | M | Y | Y | C | V | R | S | I | Y | Y | Y | G | S | S | P | F | D | F | W | G | Q | G | T | T | L | T | V | S | A |
Light chain
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 30A | 30B | 30C | 30D | 30E | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 |
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D | I | V | M | T | Q | A | T | S | S | V | P | V | T | P | G | E | S | V | S | I | S | C | R | S | S | K | S | L | L | H | S | N | G | N | T | Y | L | Y | W | F | L | Q | R | P | G | Q | S | P | Q | L | L | I | Y | R | M | S | N | L | A | S | G | V | P | D | R | F | S | G | S | G | S | G | T | A | F | T | L | T | I | S | R | L | E | A | E | D | V | G | V | Y | Y | C | M | Q | H | L | E | Y | P | L | T | F | G | A | G | T | K | L | E |
Antigen Details | |
---|---|
Antigen chains | A |
Antigen type | protein |
Antigen name | guanine nucleotide-binding protein g(s) subunit alphaisoforms short |
Antigen species | HOMO SAPIENS |
Antigen sequence | MMGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYELL |
CDR Sequences (chothia definition) | |
---|---|
CDRH1 | GFAFSSF |
CDRH2 | SSGSGT |
CDRH3 | SIYYYGSSPFDF |
CDRL1 | RSSKSLLHSNGNTYLY |
CDRL2 | RMSNLAS |
CDRL3 | MQHLEYPLT |
Orientation Angles (from ABangle) | |
---|---|
HL | -64.38° |
HC1 | 73.41° |
HC2 | 118.92° |
LC1 | 120.36° |
LC2 | 83.67° |
dc | 16.83Å |
Fv Details | |
---|---|
Heavy chain | N |
Light chain | |
Heavy subgroup | IGHV3 |
Light subgroup | NA |
Species | SYNTHETIC CONSTRUCT |
In complex? | True |
scFv? | False |
Has constant domain? | False |
Numbered Sequences (chothia) |
---|
Heavy chain
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 52A | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 82A | 82B | 82C | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 100A | 100B | 100C | 100D | 100E | 100F | 100G | 100H | 100I | 100J | 100K | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | 109 | 110 | 111 | 112 | 113 |
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Q | V | Q | L | Q | E | S | G | G | G | L | V | Q | P | G | G | S | L | R | L | S | C | A | A | S | G | F | T | F | S | N | Y | K | M | N | W | V | R | Q | A | P | G | K | G | L | E | W | V | S | D | I | S | Q | S | G | A | S | I | S | Y | T | G | S | V | K | G | R | F | T | I | S | R | D | N | A | K | N | T | L | Y | L | Q | M | N | S | L | K | P | E | D | T | A | V | Y | Y | C | A | R | C | P | A | P | F | T | R | D | C | F | D | V | T | S | T | T | Y | A | Y | R | G | Q | G | T | Q | V | T | V | S | S |
Antigen Details | |
---|---|
Antigen chains | A,B |
Antigen type | protein |
Antigen name | guanine nucleotide-binding protein g(s) subunit alphaisoforms short; guanine nucleotide-binding protein g(i)/g(s)/g(t) subunitbeta-1 |
Antigen species | HOMO SAPIENS |
Antigen sequence | MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN/MMGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYELL |
CDR Sequences (chothia definition) | |
---|---|
CDRH1 | GFTFSNY |
CDRH2 | SQSGAS |
CDRH3 | CPAPFTRDCFDVTSTTYAY |
Schneider, C., Raybould, M.I.J., Deane, C.M. (2022) SAbDab in the Age of Biotherapeutics: Updates including SAbDab-Nano, the Nanobody Structure Tracker. Nucleic Acids Res. 50(D1):D1368-D1372 [link]
SAbDab paper: Dunbar, J., Krawczyk, K. et al (2014). Nucleic Acids Res. 42. D1140-D1146 [link]
Thera-SAbDab paper: Raybould, M.I.J., Marks, C. et al (2019). Nucleic Acids Res. gkz827 [link]