SCALOP

Sequence-based antibody CAnonical LOoP structure annotation.

• SCALOP is a sequence-based canonical form predictor for five of the six complementarity-determining regions (H1, H2, L1, L2 and L3) on an antibody.
• Submit a single or multiple sequence(s) through the form below.
• The sequences will be numbered by ANARCI and assigned to canonical forms by SCALOP.
• Results are displayed by the name of the assigned clusters and the PDB-chain identifier from which the median structure of the assigned cluster is extracted. (Load an example result)
• Where no result is shown for a given sequence, it is not considered an antibody by ANARCI, thus no assignment is made.
• If the user supplies a framework structure (in the same numbering scheme), CDR loop structures are selected from the members of the assigned cluster and grafted onto the supplied framework.
• SCALOP does not detect clashes nor predict side chain conformations in the grafted loops. Please use ABodyBuilder to generate a complete model.

The SCALOP Python package is freely available on Github.

Submit a single antibody chain sequence (or two chains separated by / ):

load example

QVQLQQPGAELVKPGASVKMSCKASGYTFTSYNMHWVKQTPGRGLEWIGAIYPGNGDTSYNQKFKGKATLTADKSSSTAYMQLSSLTSEDSAVYYCARSTYYGGDWYFNVWGAGTTVTVSA/QIVLSQSPAILSASPGEKVTMTCRASSSVSYIHWFQQKPGSSPKPWIYATSNLASGVPVRFSGSGSGTSYSLTISRVEAEDAATYYCQQWTSNPPTFGGGTKHELK

or upload multiple sequences as a fasta file:

Choose a numbering scheme:

IMGT Chothia

Choose a CDR definition:

North IMGT Chothia

Upload a framework structure (optional):

download example

Heavy chain ID:
Light chain ID:

Assign