Extending the Half-Life of a Fab Fragment Through Generation of a Humanised anti-Human Serum albumin (Hsa) Fv Domain: an investigation into the Correlation Between affinity and Serum Half-Life
| PDB | 5fuo |
| Species | HOMO SAPIENS |
| Method | X-RAY DIFFRACTION |
| Resolution | 3.6Å |
| Number of Fvs | 1 |
| In complex | True |
| Light chain type | Kappa |
| Has constant region | True |
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Display options: |
This PDB has 1 Fv(s).
| Fv Details | |
|---|---|
| Heavy chain | H |
| Light chain | L |
| Heavy subgroup | IGHV3 |
| Light subgroup | IGKV1 |
| Species | HOMO SAPIENS |
| In complex? | True |
| scFv? | False |
| Has constant domain? | True |
| Numbered Sequences (chothia) |
|---|
Heavy chain
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Light chain
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| Antigen Details | |
|---|---|
| Antigen chains | |
| Antigen type | |
| Antigen name | |
| Antigen species | SYNTHETIC CONSTRUCT |
| Antigen sequence | |
| CDR Sequences (chothia definition) | |
|---|---|
| CDRH1 | GIDLSNY |
| CDRH2 | WASGT |
| CDRH3 | TVPGYSTAPYFDL |
| CDRL1 | QSSPSVWSNFLS |
| CDRL2 | EASKLTS |
| CDRL3 | GGGYSSISDTT |
| Orientation Angles (from ABangle) | |
|---|---|
| HL | -63.25° |
| HC1 | 72.44° |
| HC2 | 120.61° |
| LC1 | 119.69° |
| LC2 | 81.62° |
| dc | 15.92Å |
Schneider, C., Raybould, M.I.J., Deane, C.M. (2022) SAbDab in the Age of Biotherapeutics: Updates including SAbDab-Nano, the Nanobody Structure Tracker. Nucleic Acids Res. 50(D1):D1368-D1372 [link]
SAbDab paper: Dunbar, J., Krawczyk, K. et al (2014). Nucleic Acids Res. 42. D1140-D1146 [link]
Thera-SAbDab paper: Raybould, M.I.J., Marks, C. et al (2019). Nucleic Acids Res. gkz827 [link]
