Structure Viewer: 7kr5

>   Structure details

Cryo-Em Structure of the Crac Channel Orai in an Open Conformation; H206a Gain-of-Function Mutation in complex with an antibody

PDB7kr5
SpeciesMUS MUSCULUS
MethodELECTRON MICROSCOPY
Resolution3.3Å
Number of Fvs3
In complexTrue
Light chain typeKappa
Has constant regionTrue
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>   Fv information

This PDB has 3 Fv(s).

>   O/P
Fv Details
Heavy chainO
Light chainP
Heavy subgroupIGHV10
Light subgroupIGKV6
SpeciesMUS MUSCULUS
In complex?True
scFv?False
Has constant domain?True

Numbered Sequences (chothia)

Heavy chain

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 52A 52B 52C 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 82A 82B 82C 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 100A 101 102 103 104 105 106 107 108 109 110 111 112 113
E V Q L V E S G G G L V Q P K G S L K L S C A A S G F T F N T Y A M H W V R Q A P G K G L E W V A R I R T K S N N Y A T Y Y A D S V K D R F T I S R D D S Q S M L Y L Q M N N L K T E D T A M Y Y C V R Q K Y G N Y F D Y W G Q G T T L T V S S

Light chain

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107
D V V M T Q S Q K F M S T S V G D R V S V T C K A S Q N V G I N V A W Y Q Q K P G Q S P K A L I N S A S Y R N S G V P D R F T G G G S G T D F T L T I N N V Q S E D L A E Y F C Q Q C N S Y P L T F G A G T K L E L R

Antigen Details
Antigen chainsF,E
Antigen typeprotein
Antigen namecalcium release-activated calcium channel protein 1
Antigen speciesDROSOPHILA MELANOGASTER
Antigen sequenceMSQSGEDLHSPTYLSWRKLQLSRAKLKASSKTSALLSGFAMVAMVEVQLDHDTNVPPGMLIAFAICTTLLVAVAMLALMISTCILPNIETVSNLHSISLVHESPHERLHWYIETAWAFSTLLGLILFLLEIAILCWVKFYDLSPPAAWSATVVLIPVMIIFMAFAIHFYRSLVSHKYEVTVSGIRELEMLKEQMEQDHLEHHNNIRNNGEGEEF/MSQSGEDLHSPTYLSWRKLQLSRAKLKASSKTSALLSGFAMVAMVEVQLDHDTNVPPGMLIAFAICTTLLVAVAMLALMISTCILPNIETVSNLHSISLVHESPHERLHWYIETAWAFSTLLGLILFLLEIAILCWVKFYDLSPPAAWSATVVLIPVMIIFMAFAIHFYRSLVSHKYEVTVSGIRELEMLKEQMEQDHLEHHNNIRNNGEGEEF

CDR Sequences (chothia definition)
CDRH1GFTFNTY
CDRH2RTKSNNYA
CDRH3QKYGNYFDY
CDRL1KASQNVGINVA
CDRL2SASYRNS
CDRL3QQCNSYPLT

Orientation Angles (from ABangle)
HL-59.34°
HC173.59°
HC2117.45°
LC1118.89°
LC282.27°
dc16.07Å
>   H/L
Fv Details
Heavy chainH
Light chainL
Heavy subgroupIGHV10
Light subgroupIGKV6
SpeciesMUS MUSCULUS
In complex?True
scFv?False
Has constant domain?True

Numbered Sequences (chothia)

Heavy chain

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 52A 52B 52C 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 82A 82B 82C 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 100A 101 102 103 104 105 106 107 108 109 110 111 112 113
E V Q L V E S G G G L V Q P K G S L K L S C A A S G F T F N T Y A M H W V R Q A P G K G L E W V A R I R T K S N N Y A T Y Y A D S V K D R F T I S R D D S Q S M L Y L Q M N N L K T E D T A M Y Y C V R Q K Y G N Y F D Y W G Q G T T L T V S S

Light chain

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107
D V V M T Q S Q K F M S T S V G D R V S V T C K A S Q N V G I N V A W Y Q Q K P G Q S P K A L I N S A S Y R N S G V P D R F T G G G S G T D F T L T I N N V Q S E D L A E Y F C Q Q C N S Y P L T F G A G T K L E L R

Antigen Details
Antigen chainsB,A
Antigen typeprotein
Antigen namecalcium release-activated calcium channel protein 1
Antigen speciesDROSOPHILA MELANOGASTER
Antigen sequenceMSQSGEDLHSPTYLSWRKLQLSRAKLKASSKTSALLSGFAMVAMVEVQLDHDTNVPPGMLIAFAICTTLLVAVAMLALMISTCILPNIETVSNLHSISLVHESPHERLHWYIETAWAFSTLLGLILFLLEIAILCWVKFYDLSPPAAWSATVVLIPVMIIFMAFAIHFYRSLVSHKYEVTVSGIRELEMLKEQMEQDHLEHHNNIRNNGEGEEF/MSQSGEDLHSPTYLSWRKLQLSRAKLKASSKTSALLSGFAMVAMVEVQLDHDTNVPPGMLIAFAICTTLLVAVAMLALMISTCILPNIETVSNLHSISLVHESPHERLHWYIETAWAFSTLLGLILFLLEIAILCWVKFYDLSPPAAWSATVVLIPVMIIFMAFAIHFYRSLVSHKYEVTVSGIRELEMLKEQMEQDHLEHHNNIRNNGEGEEF

CDR Sequences (chothia definition)
CDRH1GFTFNTY
CDRH2RTKSNNYA
CDRH3QKYGNYFDY
CDRL1KASQNVGINVA
CDRL2SASYRNS
CDRL3QQCNSYPLT

Orientation Angles (from ABangle)
HL-58.99°
HC173.28°
HC2117.21°
LC1118.95°
LC282.4°
dc16.04Å
>   M/N
Fv Details
Heavy chainM
Light chainN
Heavy subgroupIGHV10
Light subgroupIGKV6
SpeciesMUS MUSCULUS
In complex?True
scFv?False
Has constant domain?True

Numbered Sequences (chothia)

Heavy chain

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 52A 52B 52C 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 82A 82B 82C 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 100A 101 102 103 104 105 106 107 108 109 110 111 112 113
E V Q L V E S G G G L V Q P K G S L K L S C A A S G F T F N T Y A M H W V R Q A P G K G L E W V A R I R T K S N N Y A T Y Y A D S V K D R F T I S R D D S Q S M L Y L Q M N N L K T E D T A M Y Y C V R Q K Y G N Y F D Y W G Q G T T L T V S S

Light chain

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107
D V V M T Q S Q K F M S T S V G D R V S V T C K A S Q N V G I N V A W Y Q Q K P G Q S P K A L I N S A S Y R N S G V P D R F T G G G S G T D F T L T I N N V Q S E D L A E Y F C Q Q C N S Y P L T F G A G T K L E L R

Antigen Details
Antigen chainsD,C
Antigen typeprotein
Antigen namecalcium release-activated calcium channel protein 1
Antigen speciesDROSOPHILA MELANOGASTER
Antigen sequenceMSQSGEDLHSPTYLSWRKLQLSRAKLKASSKTSALLSGFAMVAMVEVQLDHDTNVPPGMLIAFAICTTLLVAVAMLALMISTCILPNIETVSNLHSISLVHESPHERLHWYIETAWAFSTLLGLILFLLEIAILCWVKFYDLSPPAAWSATVVLIPVMIIFMAFAIHFYRSLVSHKYEVTVSGIRELEMLKEQMEQDHLEHHNNIRNNGEGEEF/MSQSGEDLHSPTYLSWRKLQLSRAKLKASSKTSALLSGFAMVAMVEVQLDHDTNVPPGMLIAFAICTTLLVAVAMLALMISTCILPNIETVSNLHSISLVHESPHERLHWYIETAWAFSTLLGLILFLLEIAILCWVKFYDLSPPAAWSATVVLIPVMIIFMAFAIHFYRSLVSHKYEVTVSGIRELEMLKEQMEQDHLEHHNNIRNNGEGEEF

CDR Sequences (chothia definition)
CDRH1GFTFNTY
CDRH2RTKSNNYA
CDRH3QKYGNYFDY
CDRL1KASQNVGINVA
CDRL2SASYRNS
CDRL3QQCNSYPLT

Orientation Angles (from ABangle)
HL-59.54°
HC173.47°
HC2117.88°
LC1119.3°
LC282.19°
dc16.16Å
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Additional links and files for download: see help for more details.

Chothia-numbered structure Download
IMGT-numbered structure Download
Non-annotated structure from the PDB Download
Summary file for this antibody Download

Schneider, C., Raybould, M.I.J., Deane, C.M. (2022) SAbDab in the Age of Biotherapeutics: Updates including SAbDab-Nano, the Nanobody Structure Tracker. Nucleic Acids Res. 50(D1):D1368-D1372 [link]

SAbDab paper: Dunbar, J., Krawczyk, K. et al (2014). Nucleic Acids Res. 42. D1140-D1146 [link]

Thera-SAbDab paper: Raybould, M.I.J., Marks, C. et al (2019). Nucleic Acids Res. gkz827 [link]

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