Novel anti-Mesothelin antibodies Enable Crystallography of the intact Mesothelin Ectodo- Main and Engineering of Potent, T Cell-Engaging Bispecific therapeutics
| PDB | 8cxc |
| Species | MUS MUSCULUS |
| Method | X-RAY DIFFRACTION |
| Resolution | 4.31Å |
| Number of Fvs | 2 |
| In complex | True |
| Light chain type | Kappa |
| Has constant region | True |
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Display options: |
This PDB has 2 Fv(s).
| Fv Details | |
|---|---|
| Heavy chain | H |
| Light chain | L |
| Heavy subgroup | IGHV3 |
| Light subgroup | IGKV1 |
| Species | MUS MUSCULUS |
| In complex? | True |
| scFv? | False |
| Has constant domain? | True |
| Numbered Sequences (chothia) |
|---|
Heavy chain
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Light chain
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| Antigen Details | |
|---|---|
| Antigen chains | M |
| Antigen type | protein |
| Antigen name | mesothelin, cleaved form |
| Antigen species | HOMO SAPIENS |
| Antigen sequence | EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALGSGLNDIFEAQKIEWHE |
| CDR Sequences (chothia definition) | |
|---|---|
| CDRH1 | GLTFRSY |
| CDRH2 | SVSGGI |
| CDRH3 | RGAAVGSFDY |
| CDRL1 | RSSQGIGSWLA |
| CDRL2 | AASSLQS |
| CDRL3 | QQYNSYPLT |
| Orientation Angles (from ABangle) | |
|---|---|
| HL | -60.66° |
| HC1 | 71.25° |
| HC2 | 119.31° |
| LC1 | 117.54° |
| LC2 | 84.59° |
| dc | 16.49Å |
| Fv Details | |
|---|---|
| Heavy chain | B |
| Light chain | A |
| Heavy subgroup | IGHV1 |
| Light subgroup | IGKV4 |
| Species | MUS MUSCULUS |
| In complex? | True |
| scFv? | False |
| Has constant domain? | False |
| Numbered Sequences (chothia) |
|---|
Heavy chain
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 | 32 | 33 | 34 | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 52A | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | 74 | 75 | 76 | 77 | 78 | 79 | 80 | 81 | 82 | 82A | 82B | 82C | 83 | 84 | 85 | 86 | 87 | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 100A | 100B | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | 109 | 110 | 111 | 112 |
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| S | V | Q | L | Q | Q | S | G | P | E | L | E | K | P | G | A | S | V | K | I | S | C | K | A | S | G | Y | S | F | T | G | Y | T | M | N | W | V | K | Q | S | H | G | K | S | L | E | W | I | G | L | I | T | P | Y | N | G | A | S | S | Y | N | Q | K | F | R | G | K | A | T | L | T | V | D | K | S | S | S | T | A | Y | M | D | L | L | S | L | T | S | E | D | S | A | V | Y | F | C | A | R | G | G | Y | D | G | R | G | F | D | Y | W | G | S | G | T | P | V | T | V | S |
Light chain
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| D | I | E | L | T | Q | S | P | A | I | M | S | A | S | P | G | E | K | V | T | M | T | C | S | A | S | S | S | V | S | Y | M | H | W | Y | Q | Q | K | S | G | T | S | P | K | R | W | I | Y | D | T | S | K | L | A | S | G | V | P | G | R | F | S | G | S | G | S | G | N | S | Y | S | L | T | I | S | S | V | E | A | E | D | D | A | T | Y | Y | C | Q | Q | W | S | K | H | P | L | T | F | G | S | G | T | K | V | E | I | K |
| Antigen Details | |
|---|---|
| Antigen chains | M |
| Antigen type | protein |
| Antigen name | mesothelin, cleaved form |
| Antigen species | HOMO SAPIENS |
| Antigen sequence | EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQAPRRPLPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQGGIPNGYLVLDLSMQEALGSGLNDIFEAQKIEWHE |
| CDR Sequences (chothia definition) | |
|---|---|
| CDRH1 | GYSFTGY |
| CDRH2 | TPYNGA |
| CDRH3 | GGYDGRGFDY |
| CDRL1 | SASSSVSYMH |
| CDRL2 | DTSKLAS |
| CDRL3 | QQWSKHPLT |
| Orientation Angles (from ABangle) | |
|---|---|
| HL | -59.06° |
| HC1 | 75.53° |
| HC2 | 115.67° |
| LC1 | 117.98° |
| LC2 | 81.03° |
| dc | 16.74Å |
Schneider, C., Raybould, M.I.J., Deane, C.M. (2022) SAbDab in the Age of Biotherapeutics: Updates including SAbDab-Nano, the Nanobody Structure Tracker. Nucleic Acids Res. 50(D1):D1368-D1372 [link]
SAbDab paper: Dunbar, J., Krawczyk, K. et al (2014). Nucleic Acids Res. 42. D1140-D1146 [link]
Thera-SAbDab paper: Raybould, M.I.J., Marks, C. et al (2019). Nucleic Acids Res. gkz827 [link]
