Hu-mAb - Results

Job ID:	Hu-mAb_example
Job name:	Hu-mAb_example
Job status:	finished!
Log file:

> Results - Heavy Chain

Job Details

V-gene selected:	HV4
Initial humanness score:	0.0
Humanness threshold:	0.835
No. mutations made:	16

Humanised Sequence

Residue No. (IMGT)

1

2

3

4

5

6

7

8

9

11

12

13

14

15

16

17

18

19

20

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35

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104

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107

108

109

110

112

113

114

115

116

117

118

119

120

121

122

123

124

125

126

127

128

Input Sequence

E

V

K

L

L

E

S

G

G

G

L

V

Q

P

G

G

S

M

R

L

S

C

A

G

S

G

F

T

F

T

D

F

Y

M

N

W

I

R

Q

P

A

G

K

A

P

E

W

L

G

F

I

R

D

K

A

K

G

Y

T

T

E

Y

N

P

S

V

K

G

R

F

T

I

S

R

D

N

T

Q

N

M

L

Y

L

Q

M

N

T

L

R

A

E

D

T

A

T

Y

Y

C

A

R

E

G

H

T

A

A

P

F

D

Y

W

G

Q

G

V

M

V

T

V

S

S

Humanised Sequence

Q

V

K

L

L

E

S

G

P

G

L

V

K

P

S

E

T

M

R

L

T

C

A

G

S

G

F

T

F

T

D

F

Y

W

N

W

I

R

Q

P

A

G

K

A

P

E

W

L

G

F

I

R

D

K

A

K

G

Y

T

T

E

Y

N

P

S

L

K

G

R

F

T

I

S

V

D

T

T

Q

N

M

F

S

L

Q

M

N

T

V

T

A

A

D

T

A

T

Y

Y

C

A

R

E

G

H

T

A

A

P

F

D

Y

W

G

Q

G

V

M

V

T

V

S

S

Mutations

	Residue Number	Original AA	Mutated AA	Humanness Score
0	-	-	-	0.000
1	39	M	W	0.045
2	1	E	Q	0.090
3	97	E	A	0.160
4	16	G	S	0.230
5	80	R	V	0.315
6	17	G	E	0.375
7	88	Y	S	0.425
8	87	L	F	0.490
9	18	S	T	0.535
10	95	R	T	0.595
11	94	L	V	0.660
12	22	S	T	0.715
13	14	Q	K	0.765
14	9	G	P	0.795
15	71	V	L	0.830
16	82	N	T	0.855

> Results - Light Chain

Job Details

V-gene selected:	KV1
Initial humanness score:	0.724
Humanness threshold:	0.842
No. mutations made:	3

Humanised Sequence

Residue No. (IMGT)

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

16

17

18

19

20

21

22

23

24

25

26

27

28

29

36

37

38

39

40

41

42

43

44

45

46

47

48

49

50

51

52

53

54

55

56

57

65

66

67

68

69

70

71

72

74

75

76

77

78

79

80

83

84

85

86

87

88

89

90

91

92

93

94

95

96

97

98

99

100

101

102

103

104

105

106

107

108

109

114

115

116

117

118

119

120

121

122

123

124

125

126

127

Input Sequence

D

I

K

M

T

Q

S

P

S

F

L

S

A

S

V

G

D

R

V

T

L

N

C

K

A

S

Q

N

I

D

K

Y

L

N

W

Y

Q

Q

K

L

G

E

S

P

K

L

L

I

Y

N

T

N

N

L

Q

T

G

I

P

S

R

F

S

G

S

G

S

G

T

D

F

T

L

T

I

S

S

L

Q

P

E

D

V

A

T

Y

F

C

L

Q

H

I

S

R

P

R

T

F

G

T

G

T

K

L

E

L

K

Humanised Sequence

D

I

K

M

T

Q

S

P

S

F

L

S

A

S

V

G

D

R

V

T

L

N

C

Q

A

S

Q

N

I

D

K

Y

L

N

W

Y

Q

Q

K

L

G

E

S

P

K

L

L

I

Y

N

T

N

N

L

Q

T

G

V

P

S

R

F

S

G

S

G

S

G

T

D

F

T

L

T

I

S

S

L

Q

P

E

D

V

A

T

Y

F

C

L

Q

H

I

S

R

P

R

T

F

G

T

G

T

K

L

E

I

K

Mutations

	Residue Number	Original AA	Mutated AA	Humanness Score
0	-	-	-	0.724
1	24	K	Q	0.788
2	126	L	I	0.818
3	71	I	V	0.846

Heavy chain results	File containing the humanised sequence and details of the mutations made for your heavy chain.
Light chain results	File containing the humanised sequence and details of the mutations made for your light chain.
Log file	The log file for this Hu-mAb job.

SAbPred paper: Dunbar, J. et al (2016). Nucleic Acids Res. 44. W474-W478 [link]