SAbDab: The Structural Antibody Database

> Structural Summary

Therapeutic	Izalontamab
Target 1	EGFR/ERBB1/HER1
Heavy Chain 1	QVQLKQSGPGLVQPSQSLSITCTVSGFSLTNYGVHWVRQSPGKGLEWLGVIWSGGNTDYNTPFTSRLSINKDNSKSQVFFKMNSLQSNDTAIYYCARALTYYDYEFAYWGQGTLVTVSS
Light Chain 1	DILLTQSPVILSVSPGERVSFSCRASQSIGTNIHWYQQRTNGSPRLLIKYASESISGIPSRFSGSGSGTDFTLSINSVESEDIADYYCQQNNNWPTTFGAGTKLELK
100% seqID Fv 1 Structure	5esq [Fvs: BA, DC], 5etu [Fvs: BA, DC], 5euk [Fvs: BA, DC], 5f88 [Fvs: BA, DC], 5ff6 [Fvs: BA, DC], 5hpm [Fvs: BA, DC], 5hyq [Fvs: BA, DC], 5i2i [Fvs: BA, DC], 5i76 [Fvs: BA, DC], 5icx [Fvs: BA, DC], 5icz [Fvs: BA, DC], 5id1 [Fvs: BA, DC], 5iop [Fvs: BA, DC], 5ir1 [Fvs: BA, DC], 5itf [Fvs: BA, DC], 5iv2 [Fvs: BA, DC], 5ivz [Fvs: BA, DC], 5t1k [Fvs: BA, DC], 5t1l [Fvs: BA, DC], 5th2 [Fvs: BA, DC]
99% seqID Fv 1 Structure	1yy8 [Fvs: BA, DC], 1yy9 [Fvs: DC], 4gw1 [Fvs: BA, DC], 4gw5 [Fvs: BA, DC], 4kro [Fvs: DC], 4krp [Fvs: DC], 5icy [Fvs: BA, DC], 5id0 [Fvs: BA, DC], 5t1m [Fvs: BA, DC], 6au5 [Fvs: BA, DC], 6axp [Fvs: BA, DC], 6ayn [Fvs: BA, DC], 6azk [Fvs: BA, DC], 6azl [Fvs: BA, DC]
95-98% seqID Fv 1 Structure	5gz0 [Fvs: BA, DC], 6arp [Fvs: BA, DC], 6aru [Fvs: CB]
Target 2	ERBB3/HER3
Heavy Chain 2	QVQLQESGGGLVKPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVANINRDGSASYYVDSVKGRFTISRDDAKNSLYLQMNSLRAEDTAVYYCARDRGVGYFDLWGRGTLVTVSS
Light Chain 2	QSALTQPASVSGSPGQSITISCTGTSSDVGGYNFVSWYQQHPGKAPKLMIYDVSDRPSGVSDRFSGSKSGNTASLIISGLQADDEADYYCSSYGSSSTHVIFGGGTKVTVL
100% seqID Fv 2 Structure	None
99% seqID Fv 2 Structure	None
95-98% seqID Fv 2 Structure	None

> SAbDab Links

100% seqID Structure [Fv 1]	5etu [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5ff6 [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5f88 [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5itf [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5ivz [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5ir1 [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5esq [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5euk [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5hpm [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5id1 [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5icx [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5hyq [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5icz [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5th2 [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5iop [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5i2i [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5t1l [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5i76 [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5iv2 [Fvs: BA, DC]
100% seqID Structure [Fv 1]	5t1k [Fvs: BA, DC]
99% seqID Structure [Fv 1]	6azk [Fvs: BA, DC]
99% seqID Structure [Fv 1]	6azl [Fvs: BA, DC]
99% seqID Structure [Fv 1]	6au5 [Fvs: BA, DC]
99% seqID Structure [Fv 1]	6ayn [Fvs: BA, DC]
99% seqID Structure [Fv 1]	6axp [Fvs: BA, DC]
99% seqID Structure [Fv 1]	5id0 [Fvs: BA, DC]
99% seqID Structure [Fv 1]	5t1m [Fvs: BA, DC]
99% seqID Structure [Fv 1]	5icy [Fvs: BA, DC]
99% seqID Structure [Fv 1]	1yy8 [Fvs: BA, DC]
99% seqID Structure [Fv 1]	1yy9 [Fvs: DC]
99% seqID Structure [Fv 1]	4gw5 [Fvs: BA, DC]
99% seqID Structure [Fv 1]	4kro [Fvs: DC]
99% seqID Structure [Fv 1]	4gw1 [Fvs: BA, DC]
99% seqID Structure [Fv 1]	4krp [Fvs: DC]
95-98% seqID Structure [Fv 1]	5gz0 [Fvs: BA, DC]
95-98% seqID Structure [Fv 1]	6arp [Fvs: BA, DC]
95-98% seqID Structure [Fv 1]	6aru [Fvs: CB]
There are no identical PDB matches to at least one Fv in this therapeutic. Click the links to build models with ABodyBuilder2: [Fv 1] [Fv 2]

> Alignments

Non-identical sequences have been numbered using the Chothia scheme and aligned to Izalontamab.
CDR positions are indicated in blue. Mismatching positions are highlighted in orange.

> 6azk: B/A

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
6azk	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	A

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
6azk	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 6azl: B/A

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
6azl	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	A

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
6azl	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 6au5: B/A

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
6au5	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	A

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
6au5	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 6ayn: B/A

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
6ayn	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	A

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
6ayn	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 6axp: B/A

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
6axp	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	A

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
6axp	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 5id0: B/A

Sequence identity: 99.11%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
5id0	E	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
5id0	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 5t1m: B/A

Sequence identity: 99.11%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
5t1m	E	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
5t1m	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 5icy: B/A

Sequence identity: 99.11%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
5icy	E	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
5icy	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 1yy8: B/A

Sequence identity: 98.23%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	-	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
1yy8	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	-	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
1yy8	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 1yy9: D/C

Sequence identity: 98.23%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	-	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
1yy9	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	-	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
1yy9	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 4gw5: B/A

Sequence identity: 98.23%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	-	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
4gw5	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	-	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
4gw5	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 4kro: D/C

Sequence identity: 98.23%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	-	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
4kro	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	-	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
4kro	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 4gw1: B/A

Sequence identity: 98.23%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	-	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
4gw1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	-	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
4gw1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 4krp: D/C

Sequence identity: 98.23%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab Fv 1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	-	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
4krp	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	-	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab Fv 1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
4krp	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 6arp: B/A

Sequence identity: 97.79%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab_Fv1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
6arp	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	D	L	T	D	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	A

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab_Fv1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
6arp	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	D	E	S	I	D	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 6aru: C/B

Sequence identity: 97.79%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab_Fv1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
6aru	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	D	L	T	D	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	A

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab_Fv1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
6aru	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	D	E	S	I	D	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> 5gz0: B/A

Sequence identity: 97.78%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
izalontamab_Fv1	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	S	Q	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	S
5gz0	Q	V	Q	L	K	Q	S	G	P	G	L	V	Q	P	G	G	S	L	S	I	T	C	T	V	S	G	F	S	L	T	N	Y	G	V	H	W	V	R	Q	S	P	G	K	G	L	E	W	L	G	V	I	W	S	G	G	N	T	D	Y	N	T	P	F	T	S	R	L	S	I	N	K	D	N	S	K	S	Q	V	F	F	K	M	N	S	L	Q	S	N	D	T	A	I	Y	Y	C	A	R	A	L	T	Y	Y	D	Y	E	F	A	Y	W	G	Q	G	T	L	V	T	V	S	-

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
izalontamab_Fv1	D	I	L	L	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K
5gz0	D	I	Q	M	T	Q	S	P	V	I	L	S	V	S	P	G	E	R	V	S	F	S	C	R	A	S	Q	S	I	G	T	N	I	H	W	Y	Q	Q	R	T	N	G	S	P	R	L	L	I	K	Y	A	S	E	S	I	S	G	I	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	S	I	N	S	V	E	S	E	D	I	A	D	Y	Y	C	Q	Q	N	N	N	W	P	T	T	F	G	A	G	T	K	L	E	L	K

> SAbPred Links

Follow these links to our prediction tools:

Model the variable domain structure with ABodyBuilder2: [Fv 1] [Fv 2]
Test for possible therapeutic developability issues with TAP: [Fv 1] [Fv 2]
Assign canonical forms by sequence with SCALOP: [Fv 1] [Fv 2]
Number the sequences in any numbering scheme with ANARCI: [Fv 1 H] [Fv 1 L] [Fv 2 H] [Fv 2 L]

> Metadata

Format	Bispecific Mixed mAb and scFv
Isotype	G1;na
Highest Clinical Trial (Aug '24)	Phase-II/III
Estimated Status	Active
Recorded Developmental Technology
INN Year Proposed	2022
INN Year Recommended	None
Companies Involved	Systimmune
Conditions Approved	na
Conditions Active	Colorectal cancers, Non-small cell lung cancers, Head and neck cancers, Esophageal squamous cell carcinoma
Conditions Discontinued	na
Notes	Arm 1 is highly identical to cetuximab. Also used as ADC with brengitecan. Chimeric: Mouse/Human

Thera-SAbDab

IZALONTAMAB