SAbDab: The Structural Antibody Database

> Structural Summary

Therapeutic	Trastuzumab
Target	ERBB2/CD340/HER2
Heavy Chain	EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSS
Light Chain	DIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIK
100% seqID Fv Structure	1fvc [Fvs: BA, DC], 1n8z [Fvs: BA], 4hkz [Fvs: BA], 4ub0 [Fvs: HL], 5xhf [Fvs: BA, DC], 5xhg [Fvs: BA, DC], 6bhz [Fvs: HL, IM], 6bi0 [Fvs: HL, IM], 6bi2 [Fvs: HL, IM], 6mh2 [Fvs: BA, DC], 6oge [Fvs: ED], 7mn8 [Fvs: DC], 7t97 [Fvs: AB, CD, EF, HL], 7t99 [Fvs: AB, CD, EF, HL], 8ti4 [Fvs: HL], 8tjf [Fvs: AB, HL]
99% seqID Fv Structure	1fve [Fvs: BA, DC], 4x4x [Fvs: AB, CD], 5tdn [Fvs: BA, DC], 6bgt [Fvs: BA], 7kbp [Fvs: CC], 7t98 [Fvs: AB, HL]
95-98% seqID Fv Structure	1fvd [Fvs: BA, DC], 3bdy [Fvs: HL], 3be1 [Fvs: HL], 4x4y [Fvs: AB, CD], 5tdn [Fvs: BA, DC], 5tdo [Fvs: BA, DC], 5tdp [Fvs: BA, DC], 6dn0 [Fvs: AB, CD], 6mxr [Fvs: AB, HL], 6mxs [Fvs: AB, HL], 6my4 [Fvs: AB, HL], 6my5 [Fvs: AB, HL]

> SAbDab Links

100% seqID Structure	6bi0 [Fvs: HL, IM]
100% seqID Structure	4hkz [Fvs: BA]
100% seqID Structure	6mh2 [Fvs: BA, DC]
100% seqID Structure	5xhg [Fvs: BA, DC]
100% seqID Structure	1n8z [Fvs: BA]
100% seqID Structure	6bi2 [Fvs: HL, IM]
100% seqID Structure	1fvc [Fvs: BA, DC]
100% seqID Structure	6bhz [Fvs: HL, IM]
100% seqID Structure	6oge [Fvs: ED]
100% seqID Structure	7mn8 [Fvs: DC]
100% seqID Structure	7t97 [Fvs: AB, CD, EF, HL]
100% seqID Structure	7t99 [Fvs: AB, CD, EF, HL]
100% seqID Structure	4ub0 [Fvs: HL]
100% seqID Structure	5xhf [Fvs: BA, DC]
100% seqID Structure	8ti4 [Fvs: HL]
100% seqID Structure	8tjf [Fvs: AB, HL]
99% seqID Structure	1fve [Fvs: BA, DC]
99% seqID Structure	4x4x [Fvs: AB, CD]
99% seqID Structure	5tdn [Fvs: BA, DC]
99% seqID Structure	6bgt [Fvs: BA]
99% seqID Structure	7kbp [Fvs: CC]
99% seqID Structure	7t98 [Fvs: AB, HL]
95-98% seqID Structure	5tdn [Fvs: BA, DC]
95-98% seqID Structure	1fvd [Fvs: BA, DC]
95-98% seqID Structure	5tdp [Fvs: BA, DC]
95-98% seqID Structure	4x4y [Fvs: AB, CD]
95-98% seqID Structure	5tdo [Fvs: BA, DC]
95-98% seqID Structure	6dn0 [Fvs: AB, CD]
95-98% seqID Structure	3be1 [Fvs: HL]
95-98% seqID Structure	3bdy [Fvs: HL]
95-98% seqID Structure	6my4 [Fvs: AB, HL]
95-98% seqID Structure	6mxs [Fvs: AB, HL]
95-98% seqID Structure	6my5 [Fvs: AB, HL]
95-98% seqID Structure	6mxr [Fvs: AB, HL]

> Alignments

Non-identical sequences have been numbered using the Chothia scheme and aligned to Trastuzumab.
CDR positions are indicated in blue. Mismatching positions are highlighted in orange.

> 1fve: B/A

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
1fve	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
1fve	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 7t98: A/B

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
7t98	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
7t98	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	C	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 4x4x: A/B

Sequence identity: 99.12%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
4x4x	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	V	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
4x4x	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	D	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6bgt: B/A

Sequence identity: 99.12%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6bgt	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	D	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6bgt	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	D	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 7kbp: C/C

Sequence identity: 99.12%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
7kbp	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	T	A	C	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
7kbp	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 5tdn: B/A

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
5tdn	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	E	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
5tdn	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	K	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 1fvd: B/A

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
1fvd	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	L	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	V	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
1fvd	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 5tdp: B/A

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
5tdp	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	E	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
5tdp	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	K	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 4x4y: A/B

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
4x4y	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	D	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	V	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
4x4y	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	D	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 5tdo: B/A

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
5tdo	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	K	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
5tdo	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	E	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6dn0: A/B

Sequence identity: 98.23%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6dn0	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	D	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	V	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6dn0	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	D	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	-

> 3be1: H/L

Sequence identity: 94.71%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
3be1	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
3be1	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	S	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 3bdy: H/L

Sequence identity: 94.71%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
3bdy	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
3bdy	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	S	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6my4: A/B

Sequence identity: 93.39%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6my4	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	W	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	M	M	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6my4	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	R	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6mxs: A/B

Sequence identity: 93.39%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6mxs	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	W	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	F	M	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6mxs	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	S	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6my5: A/B

Sequence identity: 93.39%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6my5	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	W	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	F	M	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6my5	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	R	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6mxr: A/B

Sequence identity: 93.39%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
trastuzumab	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6mxr	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	W	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	M	M	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
trastuzumab	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6mxr	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	S	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> SAbPred Links

Follow these links to our prediction tools:

Model the variable domain structure with ABodyBuilder2
Test for possible therapeutic developability issues with TAP
Assign canonical forms by sequence with SCALOP
Number the sequences in any numbering scheme with ANARCI: [heavy] [light]

> Metadata

Format	Whole mAb
Isotype	G1
Highest Clinical Trial (Aug '24)	Approved
Estimated Status (Aug '24)	NFD
Recorded Developmental Technology
INN Year Proposed	1997
INN Year Recommended	1998
Companies Involved	Roche
Conditions Approved	Breast cancer
Conditions Active	na
Conditions Discontinued	na
Notes	Timigutuzumab is a biosimilar of Trastuzumab and Coprelotamab with an identical Variable region. Cinrebafusp and Anvatabart have identical V domains. Also found as an ADC with brengitacan.

Thera-SAbDab

TRASTUZUMAB