SAbDab: The Structural Antibody Database

> Structural Summary

Therapeutic	Zanidatamab
Target 1	ERBB2/CD340/HER2
Heavy Chain 1	EVQLVESGGGLVQPGGSLRLSCAASGFTFADYTMDWVRQAPGKGLEWVGDVNPNSGGSIYNQRFKGRFTFSVDRSKNTLYLQMNSLRAEDTAVYYCARNLGPSFYFDYWGQGTLVTVSS
Light Chain 1	DIQMTQSPSSLSASVGDRVTITCKASQDVSIGVAWYQQKPGKAPKLLIYSASYRYTGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQYYIYPATFGQGTKVEIK
100% seqID Fv 1 Structure	None
99% seqID Fv 1 Structure	None
95-98% seqID Fv 1 Structure	None
Target 2	ERBB2/CD340/HER2
Heavy Chain 2	EVQLVESGGGLVQPGGSLRLSCAASGFNIKDTYIHWVRQAPGKGLEWVARIYPTNGYTRYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRWGGDGFYAMDYWGQGTLVTVSS
Light Chain 2	DIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQHYTTPPTFGQGTKVEIK
100% seqID Fv 2 Structure	1fvc [Fvs: BA, DC], 1n8z [Fvs: BA], 4hkz [Fvs: BA], 4ub0 [Fvs: HL], 5xhf [Fvs: BA, DC], 5xhg [Fvs: BA, DC], 6bhz [Fvs: HL, IM], 6bi0 [Fvs: HL, IM], 6bi2 [Fvs: HL, IM], 6mh2 [Fvs: BA, DC], 6oge [Fvs: ED], 7mn8 [Fvs: DC], 7t97 [Fvs: AB, CD, EF, HL], 7t99 [Fvs: AB, CD, EF, HL], 8ti4 [Fvs: HL], 8tjf [Fvs: AB, HL]
99% seqID Fv 2 Structure	1fve [Fvs: BA, DC], 4x4x [Fvs: AB, CD], 5tdn [Fvs: BA, DC], 6bgt [Fvs: BA], 7kbp [Fvs: CC], 7t98 [Fvs: AB, HL]
95-98% seqID Fv 2 Structure	1fvd [Fvs: BA, DC], 3bdy [Fvs: HL], 3be1 [Fvs: HL], 4x4y [Fvs: AB, CD], 5tdn [Fvs: BA, DC], 5tdo [Fvs: BA, DC], 5tdp [Fvs: BA, DC], 6dn0 [Fvs: AB, CD], 6mxr [Fvs: AB, HL], 6mxs [Fvs: AB, HL], 6my4 [Fvs: AB, HL], 6my5 [Fvs: AB, HL]

> SAbDab Links

100% seqID Structure [Fv 2]	6bi0 [Fvs: HL, IM]
100% seqID Structure [Fv 2]	4hkz [Fvs: BA]
100% seqID Structure [Fv 2]	6mh2 [Fvs: BA, DC]
100% seqID Structure [Fv 2]	5xhg [Fvs: BA, DC]
100% seqID Structure [Fv 2]	1n8z [Fvs: BA]
100% seqID Structure [Fv 2]	6bi2 [Fvs: HL, IM]
100% seqID Structure [Fv 2]	1fvc [Fvs: BA, DC]
100% seqID Structure [Fv 2]	6bhz [Fvs: HL, IM]
100% seqID Structure [Fv 2]	6oge [Fvs: ED]
100% seqID Structure [Fv 2]	7mn8 [Fvs: DC]
100% seqID Structure [Fv 2]	7t97 [Fvs: AB, CD, EF, HL]
100% seqID Structure [Fv 2]	7t99 [Fvs: AB, CD, EF, HL]
100% seqID Structure [Fv 2]	4ub0 [Fvs: HL]
100% seqID Structure [Fv 2]	5xhf [Fvs: BA, DC]
100% seqID Structure [Fv 2]	8ti4 [Fvs: HL]
100% seqID Structure [Fv 2]	8tjf [Fvs: AB, HL]
99% seqID Structure [Fv 2]	1fve [Fvs: BA, DC]
99% seqID Structure [Fv 2]	4x4x [Fvs: AB, CD]
99% seqID Structure [Fv 2]	5tdn [Fvs: BA, DC]
99% seqID Structure [Fv 2]	6bgt [Fvs: BA]
99% seqID Structure [Fv 2]	7kbp [Fvs: CC]
99% seqID Structure [Fv 2]	7t98 [Fvs: AB, HL]
95-98% seqID Structure [Fv 2]	5tdn [Fvs: BA, DC]
95-98% seqID Structure [Fv 2]	1fvd [Fvs: BA, DC]
95-98% seqID Structure [Fv 2]	5tdp [Fvs: BA, DC]
95-98% seqID Structure [Fv 2]	4x4y [Fvs: AB, CD]
95-98% seqID Structure [Fv 2]	5tdo [Fvs: BA, DC]
95-98% seqID Structure [Fv 2]	6dn0 [Fvs: AB, CD]
95-98% seqID Structure [Fv 2]	3be1 [Fvs: HL]
95-98% seqID Structure [Fv 2]	3bdy [Fvs: HL]
95-98% seqID Structure [Fv 2]	6my4 [Fvs: AB, HL]
95-98% seqID Structure [Fv 2]	6mxs [Fvs: AB, HL]
95-98% seqID Structure [Fv 2]	6my5 [Fvs: AB, HL]
95-98% seqID Structure [Fv 2]	6mxr [Fvs: AB, HL]
There are no identical PDB matches to at least one Fv in this therapeutic. Click the links to build models with ABodyBuilder2: [Fv 1] [Fv 2]

> Alignments

Non-identical sequences have been numbered using the Chothia scheme and aligned to Zanidatamab.
CDR positions are indicated in blue. Mismatching positions are highlighted in orange.

> 1fve: B/A

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab Fv 2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
1fve	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab Fv 2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
1fve	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 7t98: A/B

Sequence identity: 99.56%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab Fv 2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
7t98	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab Fv 2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
7t98	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	C	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 4x4x: A/B

Sequence identity: 99.12%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab Fv 2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
4x4x	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	V	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab Fv 2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
4x4x	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	D	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6bgt: B/A

Sequence identity: 99.12%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab Fv 2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6bgt	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	D	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab Fv 2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6bgt	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	D	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 7kbp: C/C

Sequence identity: 99.12%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab Fv 2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
7kbp	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	T	A	C	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab Fv 2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
7kbp	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 5tdn: B/A

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
5tdn	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	E	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
5tdn	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	K	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 1fvd: B/A

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
1fvd	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	L	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	V	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
1fvd	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 5tdp: B/A

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
5tdp	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	E	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
5tdp	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	K	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 4x4y: A/B

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
4x4y	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	D	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	V	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
4x4y	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	D	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 5tdo: B/A

Sequence identity: 98.68%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
5tdo	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	K	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
5tdo	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	E	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	E	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6dn0: A/B

Sequence identity: 98.23%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6dn0	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	D	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	V	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6dn0	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	D	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	-

> 3be1: H/L

Sequence identity: 94.71%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
3be1	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
3be1	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	S	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 3bdy: H/L

Sequence identity: 94.71%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
3bdy	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
3bdy	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	S	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6my4: A/B

Sequence identity: 93.39%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6my4	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	W	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	M	M	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6my4	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	R	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6mxs: A/B

Sequence identity: 93.39%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6mxs	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	W	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	F	M	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6mxs	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	S	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6my5: A/B

Sequence identity: 93.39%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6my5	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	W	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	F	M	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6my5	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	R	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> 6mxr: A/B

Sequence identity: 93.39%

Heavy chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	52A	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	82A	82B	82C	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	100A	100B	100C	101	102	103	104	105	106	107	108	109	110	111	112	113
zanidatamab_Fv2	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	Y	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	D	G	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S
6mxr	E	V	Q	L	V	E	S	G	G	G	L	V	Q	P	G	G	S	L	R	L	S	C	A	A	S	G	F	N	I	K	D	T	W	I	H	W	V	R	Q	A	P	G	K	G	L	E	W	V	A	R	I	Y	P	T	N	G	Y	T	R	Y	A	D	S	V	K	G	R	F	T	I	S	A	D	T	S	K	N	T	A	Y	L	Q	M	N	S	L	R	A	E	D	T	A	V	Y	Y	C	S	R	W	G	G	M	M	F	Y	A	M	D	Y	W	G	Q	G	T	L	V	T	V	S	S

Light chain

Position	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24	25	26	27	28	29	30	30A	30B	30C	30D	31	32	33	34	35	36	37	38	39	40	41	42	43	44	45	46	47	48	49	50	51	52	53	54	55	56	57	58	59	60	61	62	63	64	65	66	67	68	69	70	71	72	73	74	75	76	77	78	79	80	81	82	83	84	85	86	87	88	89	90	91	92	93	94	95	96	97	98	99	100	101	102	103	104	105	106	107
zanidatamab_Fv2	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	V	N	-	-	-	-	T	A	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	S	A	S	F	L	Y	S	G	V	P	S	R	F	S	G	S	R	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K
6mxr	D	I	Q	M	T	Q	S	P	S	S	L	S	A	S	V	G	D	R	V	T	I	T	C	R	A	S	Q	D	I	P	R	S	I	S	G	Y	V	A	W	Y	Q	Q	K	P	G	K	A	P	K	L	L	I	Y	W	G	S	Y	L	Y	S	G	V	P	S	R	F	S	G	S	G	S	G	T	D	F	T	L	T	I	S	S	L	Q	P	E	D	F	A	T	Y	Y	C	Q	Q	H	Y	T	T	P	P	T	F	G	Q	G	T	K	V	E	I	K

> SAbPred Links

Follow these links to our prediction tools:

Model the variable domain structure with ABodyBuilder2: [Fv 1] [Fv 2]
Test for possible therapeutic developability issues with TAP: [Fv 1] [Fv 2]
Assign canonical forms by sequence with SCALOP: [Fv 1] [Fv 2]
Number the sequences in any numbering scheme with ANARCI: [Fv 1 H] [Fv 1 L] [Fv 2 H] [Fv 2 L]

> Metadata

Format	Bispecific mAb
Isotype	G1;G1
Highest Clinical Trial (Aug '24)	Preregistration
Estimated Status	Active
Recorded Developmental Technology
INN Year Proposed	2019
INN Year Recommended	2020
Companies Involved	Zymeworks
Conditions Approved	na
Conditions Active	Gastric cancer, Oesophageal cancer, Breast cancer, Solid tumours
Conditions Discontinued	Ovarian cancer
Notes	Biparatopic Bispecific. Humanised

Thera-SAbDab

ZANIDATAMAB